Abstract：
:The availability of a complete genome database for the cyanobacterium Synechocystis sp. PCC6803 (glucose-tolerant strain) has raised expectations that this organism would become a reference strain for work aimed at understanding the CO(2)-concentrating mechanism (CCM) in cyanobacteria. However, the amount of physiological data available has been relatively limited. In this report we provide data on the relative contributions of net HCO(3) (-) uptake and CO(2) uptake under steady state photosynthetic conditions. Cells were compared after growth at high CO(2) (2% v/v in air) or limiting CO(2) conditions (20 ppm CO(2)). Synechocystis has a very high dependence on net HCO(3) (-) uptake at low to medium concentrations of inorganic carbon (Ci). At high Ci concentrations net CO(2) uptake became more important but did not contribute more than 40% to the rate of photosynthetic O(2) evolution. The data also confirm that high Ci cells of Synechocystis sp. PCC6803 possess a strong capacity for net HCO(3) (-) uptake under steady state photosynthetic conditions. Time course experiments show that induction of maximal Ci uptake capacity on a shift from high CO(2) to low CO(2) conditions was near completion by four hours. By contrast, relaxation of the induced state on return of cells to high CO(2), takes in excess of 230 h. Experiments were conducted to determine if Synechocystis sp. PCC6803 is able to exhibit a 'fast induction' response under severe Ci limitation and whether glucose was capable of causing a rapid inactivation in Ci uptake capacity. Clear evidence for either response was not found.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Benschop JJ,Badger MR,Dean Price Gdoi
10.1023/A:1025850230977subject
Has Abstractpub_date
2003-01-01 00:00:00pages
117-26issue
2-3eissn
0166-8595issn
1573-5079journal_volume
77pub_type
杂志文章abstract：:The PAM-2000 portable chlorophyll fluorometer represents one of the first commercially available instruments utilizing the Pulse Amplitude Modulation (PAM) measurement principle, and has become a widely used platform for measuring chlorophyll fluorescence in a wide range of study systems. In this paper, we describe a ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-9010-3
更新日期：2006-03-01 00:00:00
abstract：:Pigment-protein-complexes of two chlorophyll b deficient mutants of Arabidopsis and from the wild type were separated electrophoretically. Light-harvesting proteins were absent in the chlorophyll b free mutant ch(1) and their amount was reduced in the mutant ch(2) which has a reduced content of chlorophyll b. The rati...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041827
更新日期：1983-01-01 00:00:00
abstract：:We generated transgenic tobacco plants with high levels of fructose-1,6-bisphosphatase expressing cyanobacterialfructose-1,6-/sedoheptulose-1,7-bisphosphatase in the cytosol. At ambient CO(2) levels (360 ppm), growth, photosynthetic activity, and fresh weight were unchanged but the sucrose/hexose/starch ratio was slig...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-011-9645-1
更新日期：2011-05-01 00:00:00
abstract：:The chlorophyll content is an important experimental parameter in agronomy and plant biology research. In this report, we explore the feasibility of determining total concentration of extracts containing chlorophyll a and chlorophyll b by chlorophyll fluorescence. We found that an excitation at 457 nm results in the s...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-012-9777-y
更新日期：2012-10-01 00:00:00
abstract：:Cross-reconstitution of the extrinsic proteins and Photosystem II (PS II) from a green alga, Chlamydomonas reinhardtii, and a higher plant,Spinacia oleracea, was performed to clarify the differences of binding properties of the extrinsic proteins between these two species of organisms. (1) Chlamydomonas PsbP and PsbQ ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-7760-y
更新日期：2005-06-01 00:00:00
abstract：:The nucleotide sequences of the genes coding for the subunits of the Photosystem I (PS I) core, PsaA and PsaB were determined for the marine prokaryotic oxyphototrophs Prochlorococcus sp. MED4 (CCMP1378), P. marinus SS120 (CCMP1375) and Synechococcus sp. WH7803. Divergence of these sequences from those of both freshwa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006445810996
更新日期：2000-01-01 00:00:00
abstract：:Peridinin-chlorophyll-protein (PCP), containing differently absorbing chlorophyll derivatives, are good models with which to study energy transfer among monomeric chlorophylls (Chls) by both bulk and single-molecule spectroscopy. They can be obtained by reconstituting the N-terminal domain of the protein (N-PCP) with ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9277-7
更新日期：2008-02-01 00:00:00
abstract：:For maximal rates of CO2 assimilation in isolated intact spinach chloroplasts the generation of the adequate NADPH/ATP ratio is achieved either by cyclic electron flow around photosystem I or by linear electron transport to oxaloacetate, nitrite or oxygen (Mehler-reaction). The interrelationships between these poising...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00019060
更新日期：1994-10-01 00:00:00
abstract：:With this Tribute, we remember and honor Thomas John (Tom) Wydrzynski. Tom was a highly innovative, independent and committed researcher, who had, early in his career, defined his life-long research goal. He was committed to understand how Photosystem II produces molecular oxygen from water, using the energy of sunlig...
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-018-0606-9
更新日期：2019-06-01 00:00:00
abstract：:We evaluate low-temperature (low-T) emission spectra of photosystem II core complexes (PSII-cc) previously reported in the literature, which are compared with emission spectra of PSII-cc obtained in this work from spinach and for dissolved PSII crystals from Thermosynechococcus (T.) elongatus. This new spectral datase...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-015-0128-7
更新日期：2015-06-01 00:00:00
abstract：:Several models have been proposed to interpret the mechanism of proton-pumping associated with the electron transfer reactions in the cytochrome b/f complex. Energetics considerations suggest that the proton pump is coupled to the oxidation of cytochrome b by plastoquinone. Experiments performed in living cells under ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029737
更新日期：1986-01-01 00:00:00
abstract：:Cyanobacteria perform photosynthesis with the use of large light-harvesting antennae called phycobilisomes (PBSs). These hemispherical PBSs contain hundreds of open-chain tetrapyrrole chromophores bound to different peptides, providing an arrangement in which excitation energy is funnelled towards the PBS core from wh...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-017-0417-4
更新日期：2018-03-01 00:00:00
abstract：:The chloroplast photo-oxidation and the expression of the Cab gene Lhcb1, encoding the Lhcb1 light-harvesting chlorophyll a/b-protein of PS II, have been studied in leaf cells of maize treated with the two bleaching herbicides norflurazon and amitrole and of the two carotenoid-free mutants vp9 and vp2 grown under high...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006477215572
更新日期：2000-01-01 00:00:00
abstract：:Flash-induced kinetics of the membrane potential increase related to electron transfer within the cytochrome (cyt) b/c1 complex (Phase III) and that of cyt c1+c2 reduction have been measured as a function of myxothiazol concentration in isolated chromatophores and whole cells of Rhodobacter sphaeroides. Upon addition ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041020
更新日期：1996-05-01 00:00:00
abstract：:Two green algal species, Chlamydomonas reinhardtii and Scenedesmus obliquus, exhibited a relative maximum during the decay of luminescence, when adapted to low CO2 conditions that was not observed in high CO2 adapted cells.From the kinetics of transient changes in the level of dark fluorescence, after illumination and...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00034857
更新日期：1990-03-01 00:00:00
abstract：:Regulation of photosynthetic electron transport at different levels of structural and functional organization of photosynthetic apparatus provides efficient performance of oxygenic photosynthesis in plants. This review begins with a brief overview of the chloroplast electron transport chain. Then two noninvasive bioph...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-015-0094-0
更新日期：2015-08-01 00:00:00
abstract：:Solubilisation of thylakoid membranes from young leaves of Pisum sativum in the presence of Triton X-100 resulted in an almost complete loss of quenching of light-harvesting chlorophyll-protein (LHCP) fluorescence, as measured at 77°K. There were concomitant changes in the kinetics of light-saturation curves of electr...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00037130
更新日期：1986-01-01 00:00:00
abstract：:The previous work in our lab showed that the spinach chloroplast ATP synthase epsilon mutant with 3 amino acid residues deleted from the N-terminus had much lower ability to inhibit ATP hydrolysis and block proton leakage in comparison to a mutant with 1 or 2 residues deleted from the N-terminus. The present study aim...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-6554-6
更新日期：2005-01-01 00:00:00
abstract：:The picture presently emerging from studies on the mechanism of photosystem II electron transport is discussed. The reactions involved in excitation trapping, charge separation and stabilization of the charge pair in the reaction center, followed by the reactions with the substrates, plastoquinone reduction and water ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032785
更新日期：1985-01-01 00:00:00
abstract：:Thylakoids are complex sub-organellar membrane systems whose role in photosynthesis makes them critical to life. Thylakoids require the coordinated expression of both nuclear- and plastid-encoded proteins to allow rapid response to changing environmental conditions. Transport of cytoplasmically synthesized proteins to...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-018-0567-z
更新日期：2018-12-01 00:00:00
abstract：:Chloroplasts of a chlorophyll (Chl) b-less barley mutant were solubilized with digitonin and fractionated by polyacrylamide gel electrophoresis with sodium deoxycholate in the running buffer. By this procedure, in contrast to using sodium dodecylsulfate (SDS) for solubilization, a Chl a-protein analogous to the major ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018272
更新日期：1987-01-01 00:00:00
abstract：:Although it has been believed that wild-type plants are capable of protecting photosystem I (PSI) under high light, our previous study indicates that PSI is sensitive to high light in the shade-established tree species Psychotria rubra. However, the underlying physiological mechanisms are unclear. In this study, we ex...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-016-0275-5
更新日期：2016-07-01 00:00:00
abstract：:The effect of leaf water potential (Ψ) on net CO2 assimilation rate (A), stomatal conductance (g), transpiration (E) and water-use efficiency (WUE) was measured for three cultivars of cacao (Theobroma cacao L.) seedlings during three recurrent drought cycles. Net assimilation varied greatly at high water potentials, b...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00037179
更新日期：1989-09-01 00:00:00
abstract：:The psaA and psaB genes of the chloroplast genome in oxygenic photosynthetic organisms code for the major peptides of the Photosystem 1 reaction center. A heterodimer of the two polypeptides PsaA and PsaB is thought to bind the reaction center chlorophyll, P700, and the early electron acceptors A0, A1 and Fe-SX. Fe-SX...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020438
更新日期：1995-11-01 00:00:00
abstract：:Since 1986, non-uniform photosynthesis over the leaf area that may be attributed to patchy stomatal closure, has been an important issue in stress physiology of photosynthesis. In this review, I first outline the gaseous environment within the intercellular spaces, because this is the most fundamental background of th...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035537
更新日期：1992-03-01 00:00:00
abstract：:To understand design principles for assembling photosynthetic biohybrids that incorporate precisely-controlled sites for electron injection into redox enzyme cofactor arrays, we investigated the influence of chirality in assembly of the photosensitizer ruthenium(II)bis(2,2'-bipyridine)(4-bromomethyl-4'-methyl-2,2'-bip...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-019-00697-8
更新日期：2020-02-01 00:00:00
abstract：:Photoacclimation by strains of Haslea "blue" diatom species H. ostrearia and H. silbo sp. nov. ined. was investigated with rapid light curves and induction-recovery curves using fast repetition rate fluorescence. Cultures were grown to exponential phase under 50 µmol m-2 s-1 photosynthetic available radiation (PAR) an...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0508-x
更新日期：2018-09-01 00:00:00
abstract：:I provide here a personal perspective on (i) the awards that were given to young investigators at the 2008 and 2009 Gordon Research Conferences on Biochemistry/Biophysics of Photosynthesis; and (ii) the ambiance at these conferences through some photographs, particularly of the 2009 conference. ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9479-2
更新日期：2009-10-01 00:00:00
abstract：:The electron transport rates of photosystems II and I, amounts of electron carriers, coupling factor activity and photosynthetic rates were investigated in thylakoids isolated from pea plants grown under a wide range of light intensities (16 h light-8 h dark). The electron transport rates of PS II and PS I, as partial...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00028525
更新日期：1984-06-01 00:00:00
abstract：:Photoinhibition of Photosystem II (PSII) in lincomycin-treated leaves begins as a first-order reaction, but fluorescence measurements have suggested that after prolonged illumination, the number of active PSII centres stabilizes to 15-20% of control. The stabilization has been interpreted to indicate that photoinhibit...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9496-1
更新日期：2010-01-01 00:00:00
