Abstract:
:The psaA and psaB genes of the chloroplast genome in oxygenic photosynthetic organisms code for the major peptides of the Photosystem 1 reaction center. A heterodimer of the two polypeptides PsaA and PsaB is thought to bind the reaction center chlorophyll, P700, and the early electron acceptors A0, A1 and Fe-SX. Fe-SX is a 4Fe4S center requiring 4 cysteine residues as ligands from the protein. As PsaA and PsaB have only three and two conserved cysteine residues respectively, it has been proposed by several groups that Fe-SX is an unusual inter-peptide center liganded by two cysteines from each peptide. This hypothesis has been tested by site directed mutagenesis of PsaA residue C575 and the adjacent D576. The C575D mutant does not assemble Photosystem 1. The C575H mutant contains a photoxidisable chlorophyll with EPR properties of P700, but no other Photosystem 1 function has been detected. The D576L mutant assembles a modified Photosystem 1 in which the EPR properties of the Fe-SA/B centers are altered. The results confirm the importance of the conserved cysteine motif region in Photosystem 1 structure.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Hallahan BJ,Purton S,Ivison A,Wright D,Evans MCdoi
10.1007/BF00020438subject
Has Abstractpub_date
1995-11-01 00:00:00pages
257-64issue
1-2eissn
0166-8595issn
1573-5079journal_volume
46pub_type
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journal_title:Photosynthesis research
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journal_title:Photosynthesis research
pub_type: 杂志文章
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更新日期:1989-08-01 00:00:00
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journal_title:Photosynthesis research
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更新日期:1996-05-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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更新日期:2005-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034834
更新日期:1988-11-01 00:00:00
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更新日期:2002-01-01 00:00:00
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更新日期:2005-06-01 00:00:00
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更新日期:2014-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/BF00024189
更新日期:1986-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s11120-007-9244-3
更新日期:2008-02-01 00:00:00
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更新日期:2008-10-01 00:00:00
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更新日期:1987-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029737
更新日期:1986-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-06-01 00:00:00
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更新日期:2005-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
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更新日期:1985-12-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF02187472
更新日期:1993-08-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章,评审
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更新日期:2015-12-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041010
更新日期:1996-05-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章,评审
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更新日期:2010-06-01 00:00:00