An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in leaf crude extracts.

Abstract:

:An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in crude extracts of leaves is proposed. It is based on the ELISA technique, and offers two modifications. A non-competitive technique has a higher sensitivity and is the right option for samples of low fructose-1,6-bisphosphatase content. However, this method is not sufficiently specific when the total protein is higher than 5 μg/cm(3); so, despite its lower sensitivity, in these circumstances a competitive technique is more suitable. Thus photosynthetic fructose-1,6-bisphosphatase can be measured without interferences from the gluconeogenic cytosolic enzyme of the photosynthetic cell or from a non-specific phosphatase present in the chloroplast.

journal_name

Photosynth Res

journal_title

Photosynthesis research

authors

Hermoso R,Chueca A,Lazaro JJ,Lopez-Gorge J

doi

10.1007/BF00032710

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

269-78

issue

3

eissn

0166-8595

issn

1573-5079

journal_volume

14

pub_type

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