Abstract：
:Two lessons I have learned during my research career are the importance of following up unexpected observations and realizing that the most obvious interpretation of such observations can be rational but wrong. When you carry out an experiment there is usually an expectation that the result will fall within a range of predictable outcomes, and it is natural to feel pleased when this turns out to be the case. In my view this response is a mistake. What you should be hoping for is a puzzling result that was not anticipated since with persistence and luck further experiments may uncover something new. In this article I give a personal account of how studies of the synthesis of proteins by isolated intact chloroplasts from pea leaves eventually led to the discovery of the chaperonins and the formulation of the general concept of the molecular chaperone function that is now seen to be a fundamental aspect of how all cells work.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
John Ellis Rdoi
10.1023/B:PRES.0000030439.62331.d0subject
Has Abstractpub_date
2004-01-01 00:00:00pages
333-43issue
1-3eissn
0166-8595issn
1573-5079journal_volume
80pub_type
杂志文章abstract：:The chloroplast genome of the highly neutral-lipid-producing marine pennate diatom Fistulifera sp. strain JPCC DA0580 was fully sequenced using high-throughput pyrosequencing. The general features and gene content were compared with three other complete diatom chloroplast genomes. The chloroplast genome is 134,918 bp ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-011-9622-8
更新日期：2011-09-01 00:00:00
abstract：:Effect of chemical oxidation by ferricyanide on bacteriochlorophyll a (BChl a) in the Fenna-Matthews-Olson protein (FMO) was studied using absorbance and fluorescence spectroscopy at ambient and cryogenic temperatures. Partially selective oxidation of pigments bound to the antenna complex was achieved and the probable...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9878-2
更新日期：2013-09-01 00:00:00
abstract：:Biohybrid light-harvesting architectures can be constructed that employ native-like bacterial photosynthetic antenna peptides as a scaffold to which synthetic chromophores are attached to augment overall spectral coverage. Synthetic bacteriochlorins are attractive to enhance capture of solar radiation in the photon-ri...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0021-9
更新日期：2014-11-01 00:00:00
abstract：:Acaryochloris marina is a unique cyanobacterium that contains chlorophyll (Chl) d as a major pigment. Because Chl d has smaller excitation energy than Chl a used in ordinary photosynthetic organisms, the energetics of the photosystems of A. marina have been the subject of interest. It was previously shown that the red...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0073-x
更新日期：2015-08-01 00:00:00
abstract：:The effects of Fe resupply to Fe-deficient plants have been investigated in hydroponically-grown sugar beet. In the short-term (24 h) after Fe resupply, major changes were observed, although de novo chlorophyll (Chl) synthesis had not begun yet. Approximately 50% of the zeaxanthin was converted into violaxanthin, wher...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000011919.35309.5e
更新日期：2004-01-01 00:00:00
abstract：:In the leaf succulent Asclepiad Hoya carnosa (L.) R. Br., CAM photosynthesis occurred under well-watered conditions, as characterized by diurnal gas exchange and changes in titratable acidity. Following 10-12 days of severe water stress, the plants shifted from CAM to a modified CAM-idling mode of metabolism. CAM-idli...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00052124
更新日期：1983-09-01 00:00:00
abstract：:We present a theoretical study of excitation dynamics in the chlorosome antenna complex of green photosynthetic bacteria based on a recently proposed model for the molecular assembly. Our model for the excitation energy transfer (EET) throughout the antenna combines a stochastic time propagation of the excitonic wave ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-9978-7
更新日期：2014-06-01 00:00:00
abstract：:Regulation of photosynthetic electron transport at different levels of structural and functional organization of photosynthetic apparatus provides efficient performance of oxygenic photosynthesis in plants. This review begins with a brief overview of the chloroplast electron transport chain. Then two noninvasive bioph...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-015-0094-0
更新日期：2015-08-01 00:00:00
abstract：:In order to evaluate genotypic variation, we measured leaf photosynthetic rate (Pn) and its associated factors for the rice diversity research set of germplasm (RDRS) selected from the Genebank in National Institute of Agrobiological Sciences (NIAS). Pn showed large genotypic variation from 11.9 to 32.1 micromol m(-2 ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9208-7
更新日期：2007-10-01 00:00:00
abstract：:A protein was isolated from membranes of the green sulfur bacterium Chlorobium tepidum. This protein was characterized by gel electrophoresis, gel filtration, analytical ultracentrifugation and amino acid sequencing. The molecular weight of the purified protein was shown to be 26 kDa by SDS-PAGE. HPLC gelfiltration, S...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000015383.58680.56
更新日期：2004-02-01 00:00:00
abstract：:The seasonal maximum in photosynthetic CO2 exchange rate (CER) and the cessation of leaf expansion in soybeans (Glycine max (L.) Merr) accompany fruiting under normal agricultural conditions. To investigate whether these phenomena were obligatively tied together, we caused early flowering of long-season varieties by i...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00034108
更新日期：1982-12-01 00:00:00
abstract：:In this communication, evidence is presented from the kinetics of QA (-) decay (where QA is the first plastoquinone electron acceptor of photosystem II) and oxygen evolution for the requirement of bicarbonate in the electron transport in a cyanobacteriumSynechocystis (Pasteur Culture Collection 6803). A large slowing ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00046879
更新日期：1988-10-01 00:00:00
abstract：:The cyanobacterial small CAB-like proteins (SCPs) are one-helix proteins with compelling similarity to the first and third transmembrane helix of proteins belonging to the CAB family of light-harvesting complex proteins in plants. The SCP proteins are transiently expressed at high light intensity and other stress cond...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9244-3
更新日期：2008-02-01 00:00:00
abstract：:Using a novel, pulsed micro-second time-resolved photoacoustic (PA) instrument, we measured thermal dissipation and energy storage (ES) in the intact cells of wild type (WT) Chlamydomonas reinhardtii, and mutants lacking either PSI or PSII reaction centers (RCs). On this time scale, the kinetic contributions of the th...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-011-9682-9
更新日期：2011-09-01 00:00:00
abstract：:Far-red light photoacclimation (FaRLiP) is a mechanism that allows some cyanobacteria to utilize far-red light (FRL) for oxygenic photosynthesis. During FaRLiP, cyanobacteria remodel photosystem (PS) I, PS II, and phycobilisomes while synthesizing Chl d, Chl f, and far-red-absorbing phycobiliproteins, and these change...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-016-0309-z
更新日期：2017-02-01 00:00:00
abstract：:The origin of the extraordinary high redox potential of P680, the primary electron donor of Photosystem II, is still unknown. Photochemically induced dynamic nuclear polarisation (photo-CIDNP) 13C magic-angle spinning (MAS) NMR is a powerful method to study primary electron donors. In order to reveal the electronic st...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-0411-0
更新日期：2005-06-01 00:00:00
abstract：:Envelope membranes were isolated by sucrose density gradient floatation centrifugation from the homogenate of cyanelles prepared from Cyanophora paradoxa. Two yellow bands were separated after 40 h of centrifugation. The buoyant density of one of the two fractions (fraction Y2) coincided with that of inner envelope me...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9156-2
更新日期：2007-07-01 00:00:00
abstract：:An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in crude extracts of leaves is proposed. It is based on the ELISA technique, and offers two modifications. A non-competitive technique has a higher sensitivity and is the right option for samples of low fructose-1,6-bi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032710
更新日期：1987-01-01 00:00:00
abstract：:The light-harvesting complex (LHC) of higher plants isolated using Triton X-100 has been studied during its transformation into a monomeric form known as CPII. The change was accomplished by gradually increasing the concentration of the detergent, sodium dodecyl sulfate (SDS). Changes in the red spectral region of the...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029727
更新日期：1986-01-01 00:00:00
abstract：:Water vapor was added differentially to the gas streams entering the cells of three makes of differential infrared carbon dioxide analysers. Analyser deflections were compared with those expected from dilution of the carbon dioxide by the additional gas. Tests were made at 0, 365, and 730 cm(3) m(-3) concentrations of...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00054104
更新日期：1985-12-01 00:00:00
abstract：:Most chloroplasts undergo changes in composition, function and structure in response to growth irradiance. However, Tradescantia albiflora, a facultative shade plant, is unable to modulate its light-harvesting components and has the same Chl a/Chl b ratios and number of functional PS II and PS I reaction centres on a ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1010681721929
更新日期：2001-01-01 00:00:00
abstract：:The pigment composition of the light-harvesting complexes of Photosystem II (LHC II) has been determined for lettuce (Lactuca sativa). In common with other members of the composite, the photosynthetic tissues of this species may contain large amounts of the carotenoid lactucaxanthin (ε, ε-carotene-3,3'-diol) in additi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029940
更新日期：1995-03-01 00:00:00
abstract：:The polypeptide composition of thylakoid membranes of the red alga Cyanidium caldarium was studied by PAGE in the presence of lithium dodecyl sulfate. The thylakoid membranes were shown to contain 65 polypeptides with mol wt from 110 to 10 kDa. PS I isolated from C. caldarium cells is composed of at least 5 components...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035678
更新日期：1991-10-01 00:00:00
abstract：:Photosystem II (PSII) catalyzes the oxidation of water to O2 at the manganese-containing, oxygen-evolving complex (OEC). Photoexcitation of PSII results in the oxidation of the OEC; four sequential oxidation reactions are required for the generation and release of molecular oxygen. Therefore, with flash illumination, ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9147-3
更新日期：2007-06-01 00:00:00
abstract：:Brownian Dynamics (BD) computer simulations were used to study electrostatic interactions between turnip cytochrome f (cyt f) and spinach plastocyanin (PC). Three different spinach PC structures were studied: The X-ray crystal structure of Xue and coworkers [(1998) Protein Sci 7:2099-2105] and the NMR structure of Mus...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9192-y
更新日期：2007-11-01 00:00:00
abstract：:We investigated the dark-to-light transition in Synechococcus sp. PCC 7942 cells by a detailed analysis of fluorescence transients induced by strong red light. The transients, recorded with high data-acquisition, revealed all the steps of the fast (OJIP; 10(-5)-1 s) and slow phase (PSM(T); 1-10(3) s), kinetically dist...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9405-7
更新日期：2009-03-01 00:00:00
abstract：:A non-competitive enzyme-linked immunosorbent assay (ELISA) which enables detection of as little as 0.1 ng cytochrome f in leaf extracts has been developed. No evidence for specific or non-specific interference by proteins other than cytochrome f was found. The assay was applied to a comparative study of age-related c...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032649
更新日期：1990-04-01 00:00:00
abstract：:The filamentous nitrogen-fixing cyanobacterium, Anabaena sp. strain PCC 7120 was found to tolerate very high doses of 60Co-gamma radiation or prolonged desiccation. Post-stress, cells remained intact and revived all the vital functions. A remarkable capacity to repair highly disintegrated genome and recycle the damage...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9936-9
更新日期：2013-10-12 00:00:00
abstract：:While interest in photosynthetic thermal acclimation has been stimulated by climate warming, comparing results across studies requires consistent terminology. We identify five types of photosynthetic adjustments in warming experiments: photosynthesis as measured at the high growth temperature, the growth temperature, ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9873-7
更新日期：2014-02-01 00:00:00
abstract：:A high CO2-requiring mutant of Synechocystis PCC6803 (G3) capable of Ci transport but unable to utilize the intracellular Ci pool for photosynthesis was constructed. A DNA clone of 6.1 kbp that transforms the G3 mutant to the wild-type phenotype was isolated from a Synechocystis PCC6803 genomic library. Complementatio...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029385
更新日期：1994-02-01 00:00:00
