Abstract：
:Two lessons I have learned during my research career are the importance of following up unexpected observations and realizing that the most obvious interpretation of such observations can be rational but wrong. When you carry out an experiment there is usually an expectation that the result will fall within a range of predictable outcomes, and it is natural to feel pleased when this turns out to be the case. In my view this response is a mistake. What you should be hoping for is a puzzling result that was not anticipated since with persistence and luck further experiments may uncover something new. In this article I give a personal account of how studies of the synthesis of proteins by isolated intact chloroplasts from pea leaves eventually led to the discovery of the chaperonins and the formulation of the general concept of the molecular chaperone function that is now seen to be a fundamental aspect of how all cells work.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
John Ellis Rdoi
10.1023/B:PRES.0000030439.62331.d0subject
Has Abstractpub_date
2004-01-01 00:00:00pages
333-43issue
1-3eissn
0166-8595issn
1573-5079journal_volume
80pub_type
杂志文章abstract：:A non-competitive enzyme-linked immunosorbent assay (ELISA) which enables detection of as little as 0.1 ng cytochrome f in leaf extracts has been developed. No evidence for specific or non-specific interference by proteins other than cytochrome f was found. The assay was applied to a comparative study of age-related c...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032649
更新日期：1990-04-01 00:00:00
abstract：:The effects of protein phosphorylation and cation depletion on the electron transport rate and fluorescence emission characteristics of photosystem I at two stages of chloroplast development in light-grown wheat leaves are examined. The light-harvesting chlorophyll a/b protein complex associated with photosystem I (LH...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00030071
更新日期：1990-01-01 00:00:00
abstract：:The early suggestion by Lozier and Butler (Photochem. Photobiol. 17, 133-137 (1973)) that EPR Signal II arises from radicals associated with the water-splitting process in PSII has been confirmed and extended over the intervening years. Recent work has identified the Signal II radicals, [Formula: see text] and [Formul...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00118308
更新日期：1986-01-01 00:00:00
abstract：:Precise control of the proton concentration gradient across thylakoid membranes (ΔpH) is essential for photosynthesis and its regulation because the gradient contributes to the generation of the proton motive force used for ATP synthesis and also for the fast and reversible induction of non-photochemical quenching (NP...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0575-z
更新日期：2019-03-01 00:00:00
abstract：:A time line of important research relating to anoxygenic photosynthetic organisms is presented. The time line includes discoveries of organisms, metabolic capabilities, molecular complexes and genetic systems. It also pinpoints important milestones in our understanding of the structure, function, organization, assembl...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000030448.24695.ec
更新日期：2004-01-01 00:00:00
abstract：:Photoinhibition of the light-induced Photosystem I (PS I) electron transfer activity from the reduced dichlorophenol indophenol to methyl viologen was studied. PS I preparations with Chl/P700 ratios of about 180 (PS I-180), 100 (PS I-100) and 40 (PS I(HA)-40) were isolated from spinach thylakoid membranes by the treat...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00016175
更新日期：1996-02-01 00:00:00
abstract：:Photosynthesis powers our planet and is a source of inspiration for developing artificial constructs mimicking many aspects of the natural energy transducing process. In the complex machinery of photosystem II (PSII), the redox activity of the tyrosine Z (Tyrz) hydrogen-bonded to histidine 190 (His190) is essential fo...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-020-00815-x
更新日期：2021-01-11 00:00:00
abstract：:The Soret absorption spectra of six synthetic rigid porphyrin dimers whose crystal structures have been determined are simulated using simple exciton theory. The objective is to test the validity of the point dipole and associated approximations; the electronic interaction parameters are thus calculated using data obt...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00048299
更新日期：1989-12-01 00:00:00
abstract：:The chlorina-f2 mutant of barley (Hordeum vulgare L.) contains no chlorophyll b in its light-harvesting antenna, whereas the chlorina-103 mutant contains approximately 10% of the chlorophyll b found in wild-type. The absolute chlorophyll antenna size for Photosystem-II in wild-type, chlorina-103 and chlorina-f2 mutant...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00146413
更新日期：1993-11-01 00:00:00
abstract：:A study of the absorption and fluorescence characteristics of the D1/D2/cytb-559 reaction centre complex of Photosystem II has been carried out by gaussian decomposition of absorption spectra both at room temperature and 72 K and of the room temperature fluorescence spectrum. A five component fit was found in which th...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02183048
更新日期：1994-09-01 00:00:00
abstract：:We have used antibodies generated against synthetic peptides to determine the topology of the 43 kD chlorophyll a binding protein (CP 43) of Photosystem II. Based on the pattern of proteolytic fragments detected (on western blots) by peptide specific antibodies, a six transmembrane span topological model, with the ami...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00019041
更新日期：1994-04-01 00:00:00
abstract：:Plants are exposed to a temporally and spatially heterogeneous environment, and photosynthesis is well adapted to these fluctuations. Understanding of the complex, non-linear dynamics of photosynthesis in fluctuating light requires novel-modeling approaches that involve not only the primary light and dark biochemical ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-007-9178-9
更新日期：2007-07-01 00:00:00
abstract：:Most chloroplasts undergo changes in composition, function and structure in response to growth irradiance. However, Tradescantia albiflora, a facultative shade plant, is unable to modulate its light-harvesting components and has the same Chl a/Chl b ratios and number of functional PS II and PS I reaction centres on a ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1010681721929
更新日期：2001-01-01 00:00:00
abstract：:Light-induced reaction dynamics of isolated photosynthetic membranes obtained from wild-type (WT) and reaction center (RC)-subunit deletion strains SPUHK1 (an H-subunit deletion mutant) and SK Delta LM (an (L+M) deletion mutant) of the purple non-sulphur bacterium Rhodospirillum rubrum have been investigated by femtos...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9520-5
更新日期：2010-03-01 00:00:00
abstract：:Rhodobacter sphaeroides 2.4.1 is an alpha-3 purple nonsulfur eubacterium with an extensive metabolic repertoire. Under anaerobic conditions, it is able to grow by photosynthesis, respiration and fermentation. Photosynthesis may be photoheterotrophic using organic compounds as both a carbon and a reducing source, or ph...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1013831823701
更新日期：2001-01-01 00:00:00
abstract：:Since 1986, non-uniform photosynthesis over the leaf area that may be attributed to patchy stomatal closure, has been an important issue in stress physiology of photosynthesis. In this review, I first outline the gaseous environment within the intercellular spaces, because this is the most fundamental background of th...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035537
更新日期：1992-03-01 00:00:00
abstract：:To investigate the impact of iron deficiency on bioenergetic pathways in Chlamydomonas, we compared growth rates, iron content, and photosynthetic parameters systematically in acetate versus CO(2)-grown cells. Acetate-grown cells have, predictably (2-fold) greater abundance of respiration components but also, counter-...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-010-9562-8
更新日期：2010-07-01 00:00:00
abstract：:Green sulfur bacteria and heliobacteria are strictly anaerobic phototrophs that have homodimeric type 1 reaction center complexes. Within these complexes, highly reducing substances are produced through an initial charge separation followed by electron transfer reactions driven by light energy absorption. In order to ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-009-9521-4
更新日期：2010-06-01 00:00:00
abstract：:The nature of interaction of cytochrome b-559 high potential (HP) with electron transport on the reducing side of photosystem II was investigated by measuring the susceptibility of cytochrome b-559HP to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) under different conditions. Submicromolar DCMU concentrations decreas...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035839
更新日期：1991-03-01 00:00:00
abstract：:The relationship between the redox state of P700, the primary donor of PS I, monitored using absorbance changes at 830 nm and photochemical energy storage in PS I reaction centers assayed with the photoacoustic method (PA) was studied in isolated PS I submembrane particles aspirated onto nitrocellulose filters. Severa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1021237026500
更新日期：2002-01-01 00:00:00
abstract：:The dependence on temperature of tryptophan fluorescence lifetime in trimeric photosystem I (PSI) complexes from cyanobacteria Synechocystis sp. PCC 6803 during the heating of pre-frozen to - 180 °C in the dark or in the light-activated preparations has been studied. Fluorescence lifetime in samples frozen in the ligh...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0595-8
更新日期：2019-03-01 00:00:00
abstract：:Here, we describe a new imaging setup able to assess in vivo photosynthetic activity. The system specifically measures time-resolved chlorophyll fluorescence in response to light. It is composed of a fast digital camera equipped with a wide-angle lens for the analysis of samples up to 10 x 10 cm, i.e. entire plants or...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9487-2
更新日期：2009-10-01 00:00:00
abstract：:We present a theoretical study of excitation dynamics in the chlorosome antenna complex of green photosynthetic bacteria based on a recently proposed model for the molecular assembly. Our model for the excitation energy transfer (EET) throughout the antenna combines a stochastic time propagation of the excitonic wave ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-9978-7
更新日期：2014-06-01 00:00:00
abstract：:The localization of isoprenoid lipids in chloroplasts, the accumulation of particular isoprenoids under high irradiance conditions, and channelling of photosynthetically fixed carbon into plastidic thylakoid isoprenoids, volatile isoprenoids, and cytosolic sterols are reviewed. During leaf and chloroplast development ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-007-9204-y
更新日期：2007-05-01 00:00:00
abstract：:Triplet state electron paramagnetic resonance (EPR) experiments have been carried out at X-band on Rb. sphaeroides R-26 reaction centers that have been reconstituted with the carotenoid, spheroidene, and exchanged with 13(2)-OH-Zn-bacteriochlorophyll a and [3-vinyl]-13(2)-OH-bacteriochlorophyll a at the monomeric, 'ac...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032823
更新日期：1993-09-01 00:00:00
abstract：:Glycolate oxidase (GO; EC 1.1.3.1) was purified from the leaves of three plant species:Amaranthus hypochondriacus L.(NAD-ME type C4 dicot),Pisum sativum L. (C3 species) andParthenium hysterophorus L. (C3-C4. intermediate). A flavin moiety was present in the enzyme from all the three species. The enzyme from the C4 pla...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02184284
更新日期：1996-03-01 00:00:00
abstract：:Envelope membranes were isolated by sucrose density gradient floatation centrifugation from the homogenate of cyanelles prepared from Cyanophora paradoxa. Two yellow bands were separated after 40 h of centrifugation. The buoyant density of one of the two fractions (fraction Y2) coincided with that of inner envelope me...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9156-2
更新日期：2007-07-01 00:00:00
abstract：:In this article I review the history of research on the Fenna-Matthews-Olson (FMO) protein with emphasis on my contributions. The FMO protein, which transfers energy from the chlorosome to the reaction center in green sulfur bacteria, was discovered in 1962 and shown to contain bacteriochlorophyll a. From the absorpti...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000030428.36950.43
更新日期：2004-01-01 00:00:00
abstract：:The 'Salada de Chiprana' (Chiprana Lake) is a hypersaline (30-73 per thousand), permanent and shallow lake of endorheic origin in a semi-arid region of the Ebro depression (Aragon, Spain). Magnesium sulfate and sodium chloride represent the main salts of this athalassohaline environment. Anoxic conditions occurred per...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1014915900644
更新日期：2002-01-01 00:00:00
abstract：:The effect of leaf water potential (Ψ) on net CO2 assimilation rate (A), stomatal conductance (g), transpiration (E) and water-use efficiency (WUE) was measured for three cultivars of cacao (Theobroma cacao L.) seedlings during three recurrent drought cycles. Net assimilation varied greatly at high water potentials, b...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00037179
更新日期：1989-09-01 00:00:00
