Abstract:
:The protonophoric uncouplers carbonyl cyanide m-chlorophenylhydrazone (CCCP), 2,3,4,5,6-pentachlorophenol (PCP) and 4,5,6,7-tetrachloro-2-trifluoromethylbenzimidazole (TTFB) inhibited the Hill reaction with K3[Fe(CN)6] (but not with SiMo) in chloroplast and cyanobacterial membranes (the I50 values were approx. 1-2, 4-6 and 0.04-0.10 μM, respectively). The inhibition is due to oxidation of the uncouplers on the Photosystem II donor side (ADRY effect) and their subsequent reduction on the acceptor side, ie. to the formation of a cyclic electron transfer chain around Photosystem II involving the uncouplers as redox carriers. The relative amplitude of nanosecond chlorophyll fluorescence in chloroplasts was increased by DCMU or HQNO and did not change upon addition of uncouplers, DBMIB or DNP-INT; the HQNO effect was not removed by the uncouplers. The uncouplers did not inhibit the electron transfer from reduced TMPD or duroquinol to methylviologen which is driven by Photosystem I. These data show that CCCP, PCP and TTFB oxidized on the Photosystem II donor side are reduced by the membrane pool of plastoquinone (Qp) which is also the electron donor for K3 [Fe(CN)6] in the Hill reaction as deduced from the data obtained in the presence of inhibitors. Inhibition of the Hill reaction by the uncouplers was maximum at the pH values corresponding to the pK of these compounds. It is suggested that the tested uncouplers serve as proton donors, and not merely as electron donors on the oxidizing side of Photosystem II.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Samuilov VD,Renger G,Paschenko VZ,Oleskin AV,Gusev MV,Gubanova ON,Vasil'ev SS,Barsky ELdoi
10.1007/BF00032300subject
Has Abstractpub_date
1995-01-01 00:00:00pages
455-65issue
3eissn
0166-8595issn
1573-5079journal_volume
46pub_type
杂志文章abstract::The oxygen production of dark-adapted Photosystem II upon illumination by a series of single-turnover flashes shows a damped period four oscillation with flash number. The damping is attributed to 'misses' resulting from a statistical probability that a reaction center fails to produce a stable charge separation after...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1016128632704
更新日期:2002-01-01 00:00:00
abstract::Light-harvesting Chl a/b protein complexes were isolated from the higher plant Sinapis alba, the green alga Chlorella fusca, and the prasinophycean alga Mantoniella squamata by mild gel electrophoresis. The energy transfer from chlorophyll b and the accessory xanthophyll was measured by means of fluoresence spectrosco...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00035234
更新日期:1987-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-008-9342-x
更新日期:2008-10-01 00:00:00
abstract::Chlorophyll fluorescence imaging has been used to analyse the response elicited in Phaseolus vulgaris after inoculation with Pseudomonas syringae pv. phaseolicola 1448A (compatible interaction) and P. syringae pv. tomato DC3000 (incompatible interaction). With the aim of modulating timing of symptom development, diffe...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9278-6
更新日期:2008-04-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1020475502933
更新日期:2002-01-01 00:00:00
abstract::In thylakoid membranes, several peptides of high MW† are present which may interfere with the study of CP1's components. Modifying Cleveland's technique [7] for limited proteolysis, we have characterized the polypeptides found in the 60 kD region. Some may result from incomplete washing of the CF1 while others come fr...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00020071
更新日期:1980-03-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0213-y
更新日期:2016-05-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9671-z
更新日期:2012-03-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-014-0002-z
更新日期:2014-07-01 00:00:00
abstract::Vyacheslav Vasilevich (V.V.) Klimov (or Slava, as most of us called him) was born on January 12, 1945 and passed away on May 9, 2017. He began his scientific career at the Bach Institute of Biochemistry of the USSR Academy of Sciences (Akademy Nauk (AN) SSSR), Moscow, Russia, and then, he was associated with the Insti...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-017-0440-5
更新日期:2018-04-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000011919.35309.5e
更新日期:2004-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032321
更新日期:1987-01-01 00:00:00
abstract::Sulphoquinovosyldiacylglycerol is a negatively charged lipid which exists in the thylakoid membrane. It is proposed that a large proportion of this acidic lipid does not form a part of the bulk lipid matrix but is closely associated with protein complexes where it is tightly bound and participates in either optimising...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029747
更新日期:1986-01-01 00:00:00
abstract::There is currently a renewed interest in developing microalgae as a source of renewable energy and fuel. Microalgae hold great potential as a source of biomass for the production of energy and fungible liquid transportation fuels. However, the technologies required for large-scale cultivation, processing, and conversi...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-011-9638-0
更新日期:2011-09-01 00:00:00
abstract::The biogenesis and oxygen-evolving activity of cyanobacterial Photosystem II (PSII) is dependent on a number of accessory proteins not found in the crystallised dimeric complex. These include Psb27, a small lipoprotein attached to the lumenal side of PSII, which has been assigned a role in regulating the assembly of t...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9712-7
更新日期:2012-02-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9192-y
更新日期:2007-11-01 00:00:00
abstract::Earlier mass spectrometric measurements, in which oxygen evolution was measured following short saturating light flashes, indicated that with a time resolution of about 30 s no form of bound water and/or an oxidation product exists up to the redox state S3 of the oxygen evolving center (R. Radmer and O. Ollinger, 1986...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00046761
更新日期:1993-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00042009
更新日期:1991-12-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-004-6554-6
更新日期:2005-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1006486307473
更新日期:2000-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF02183048
更新日期:1994-09-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-013-9936-9
更新日期:2013-10-12 00:00:00
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journal_title:Photosynthesis research
pub_type: 传,历史文章
doi:10.1007/s11120-014-9999-2
更新日期:2014-06-01 00:00:00
abstract::Using 77 K chlorophyll a (Chl a) fluorescence spectra in vivo, the development was studied of Photosystems II (PS II) and I (PS I) during greening of barley under intermittent light followed by continuous light at low (LI, 50 mumol m(-2) s(-1)) and high (HI, 1000 mumol m(-2) s(-1)) irradiances. The greening at HI inte...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1020943107318
更新日期:2002-01-01 00:00:00
abstract::Bill Arnold's concept of photosynthesis as a solid state, as opposed to solution, phenomenon led him to an amazing series of fundamental discoveries. ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00040990
更新日期:1996-05-01 00:00:00
abstract::In this communication, evidence is presented from the kinetics of QA (-) decay (where QA is the first plastoquinone electron acceptor of photosystem II) and oxygen evolution for the requirement of bicarbonate in the electron transport in a cyanobacteriumSynechocystis (Pasteur Culture Collection 6803). A large slowing ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00046879
更新日期:1988-10-01 00:00:00
abstract::As part of an ongoing investigation into the effects of long-term UV-B radiation exposure on the growth and morphology of woody perennials, the gas exchange and photosynthesis of five common deciduous tree species were measured. All five tree species had been exposed to UV-B radiation for 5 years, in the field, at an ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1010620228989
更新日期:2001-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1011818021875
更新日期:2001-01-01 00:00:00
abstract::Pigment-protein-complexes of two chlorophyll b deficient mutants of Arabidopsis and from the wild type were separated electrophoretically. Light-harvesting proteins were absent in the chlorophyll b free mutant ch(1) and their amount was reduced in the mutant ch(2) which has a reduced content of chlorophyll b. The rati...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041827
更新日期:1983-01-01 00:00:00
abstract::Recommendations are given for naming of genes coding for reaction center, antenna and electron transport proteins in green photosynthetic bacteria and heliobacteria. ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF02184142
更新日期:1994-07-01 00:00:00