Abstract:
:Optically detected magnetic resonance of chlorosome-containing membranes from the green filamentous bacterium Chloroflexus aurantiacus has been performed both by fluorescence and absorption detection. Triplet states localized in the chlorosomes and in the B808-866 complex have been characterized. After chemical reduction with ascorbate followed by illumination at 200 K, recombination triplet state localized in the primary donor becomes largely populated under illumination at low temperature while all the antenna triplet states, which are localized in carotenoids and BChl a molecules, are strongly quenched. We were able to obtain the T-S spectrum of the primary donor P870 surrounded by all the antenna complexes connected to the RC via energy transfer and then in its intact environment. We found clear spectroscopic evidence for exciton interaction between the RC and the B808-866 antenna complex. This evidence was provided by the comparison of the T-S spectrum of P870 in the membranes with that of isolated RC. The analogy of some features of the difference spectra with those previously found in the same kind of experiments for Rb. sphaeroides, allows to predict a similar coupling among the primary donor and the nearby antenna BChl a molecules, assembled as circular aggregate.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Bordignon E,Scarzello M,Agostini G,Giacometti G,Vianelli A,Vannini C,Carbonera Ddoi
10.1023/A:1014947412940subject
Has Abstractpub_date
2002-01-01 00:00:00pages
45-57issue
1-2eissn
0166-8595issn
1573-5079journal_volume
71pub_type
杂志文章abstract::The electron transport rates of photosystems II and I, amounts of electron carriers, coupling factor activity and photosynthetic rates were investigated in thylakoids isolated from pea plants grown under a wide range of light intensities (16 h light-8 h dark). The electron transport rates of PS II and PS I, as partial...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00028525
更新日期:1984-06-01 00:00:00
abstract::We present here our tribute to an outstanding unique scientist, a pioneer of plant biochemistry, best known for his seminal ground-breaking discoveries related to electron and proton transport, and their intimate relation to ATP synthesis in chloroplasts. His dedication and in-depth understanding of science was matche...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-017-0380-0
更新日期:2017-06-01 00:00:00
abstract::This contribution gives a brief survey of the short history since the discovery of the aerobic phototrophic bacteria to focus on a general evolutionary scenario. Most of the citations are of reviews that have covered the earlier literature and to which the reader is directed at appropriate places in the following text...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1020493518379
更新日期:2002-01-01 00:00:00
abstract::We present here the life and work of Shmuel Malkin (1934-2017), an accomplished scientist and a gifted musician who touched the lives of many around the world. His early scientific work addressed the dynamics of light harvesting and electron transport in photosynthesis. Later, he used photoacoustic and photothermal me...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-018-0478-z
更新日期:2018-07-01 00:00:00
abstract::The oxygen production of dark-adapted Photosystem II upon illumination by a series of single-turnover flashes shows a damped period four oscillation with flash number. The damping is attributed to 'misses' resulting from a statistical probability that a reaction center fails to produce a stable charge separation after...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1016128632704
更新日期:2002-01-01 00:00:00
abstract::A hypothesis is proposed to explain the function of Cl(-) in activating the oxygenevolving complex (OEC) of photosystem II (PS II), based on the results of recent (35)Cl-NMR studies. The putative mechanism involves Cl(-) binding to two types of sites. An intrinsic site is suggested to be composed of three histidyl res...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029400
更新日期:1987-09-01 00:00:00
abstract::Heliobacteria contain Type I reaction centers (RCs) and a homodimeric core, but unlike green sulfur bacteria, they do not contain an extended antenna system. Given their simplicity, the heliobacterial RC (HbRC) should be ideal for the study of a prototypical homodimeric RC. However, there exist enormous gaps in our kn...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-007-9162-4
更新日期:2007-04-01 00:00:00
abstract::Historically, two main concepts guided research into possible mechanisms of light-induced atomic rearrangements in oxygenic photosynthesis: Photodecomposition of CO2 and photodecomposition of water. Both concepts envisioned photoinduced transfers of cumbersome whole atoms and not, as is currently held, photoinduced el...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00036216
更新日期:1991-09-01 00:00:00
abstract::Bill Arnold's concept of photosynthesis as a solid state, as opposed to solution, phenomenon led him to an amazing series of fundamental discoveries. ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00040990
更新日期:1996-05-01 00:00:00
abstract::The interaction of PsaC with the core heterodimer of Photosystem 1 was studied in wild type Synechocystis sp. PCC 6803 and the site-directed mutant R561E of PsaB. The mutant reaction center was much less stable in urea and the functional reconstitution of the mutant core using PsaC was impaired. However, the extent of...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00018261
更新日期:1994-12-01 00:00:00
abstract::Glycolate oxidase (GO; EC 1.1.3.1) was purified from the leaves of three plant species:Amaranthus hypochondriacus L.(NAD-ME type C4 dicot),Pisum sativum L. (C3 species) andParthenium hysterophorus L. (C3-C4. intermediate). A flavin moiety was present in the enzyme from all the three species. The enzyme from the C4 pla...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF02184284
更新日期:1996-03-01 00:00:00
abstract::The activity of ribulose-1,5-bisphosphate carboxylase/oxygenase fromEuglena gracilis decays steadily when exposed to agents that induce oxidative modification of cysteine residues (Cu(2+), benzofuroxan, disulfides, arsenite, oxidized ascorbate). Inactivation takes place with a concomitant loss of cysteine sulfhydryl g...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF02185411
更新日期:1993-01-01 00:00:00
abstract::Photosynthesis was well-established on the earth at least 3.5 thousand million years ago, and it is widely believed that these ancient organisms had similar metabolic capabilities to modern cyanobacteria. This requires that development of two photosystems and the oxygen evolution capability occurred very early in the...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:
更新日期:1992-01-01 00:00:00
abstract::Here, we describe a new imaging setup able to assess in vivo photosynthetic activity. The system specifically measures time-resolved chlorophyll fluorescence in response to light. It is composed of a fast digital camera equipped with a wide-angle lens for the analysis of samples up to 10 x 10 cm, i.e. entire plants or...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-009-9487-2
更新日期:2009-10-01 00:00:00
abstract::Species in the Laxa and Grandia groups of the genus Panicum are adapted to low, wet areas of tropical and subtropical America. Panicum milioides is a species with C3 photosynthesis and low apparent photorespiration and has been classified as a C3/C4 intermediate. Other species in the Laxa group are C3 with normal phot...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00024189
更新日期:1986-01-01 00:00:00
abstract::This review presents a summary of recent investigations examining the structure and function of the chlorophyll-proteins CPa-1 (CP47) and CPa-2 (CP43). Comparisons of the derived amino acid sequences of these proteins suggest sites for chlorophyll binding and for interactions between these chlorophyll-proteins and oth...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032639
更新日期:1990-04-01 00:00:00
abstract::Flash-induced absorbance measurements at 830 nm on both nanosecond and microsecond timescales have been used to characterise the effect of ultraviolet light on Photosystem II core particles. A combination of UV-A and UV-B, closely simulating the spectrum of sunlight below 350 nm, was found to have a primary effect on ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029424
更新日期:1996-07-01 00:00:00
abstract::A rapid procedure has been developed for the isolation of the photosystem two reaction centre complex (PS II RC) from a double mutant of Chlamydomonas reinhardtii, F54-14, which lacks the Photosystem one complex and the chloroplast ATPase. Thylakoid membranes are solubilised with 1.5% (w/v) Triton X-100 and the PS II ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00042974
更新日期:1995-02-01 00:00:00
abstract::Maximum photosynthetic CO2 exchange rates (Pn) of single attached leaves were determined for several cassava cultivars selected from different habitats and grown in pots outdoors at CIAT, Colombia, S.A. Pn rates were in a narrow range of 22 to 26 μmol CO2 m(-2)s(-1) for all cultivars tested when measured at high photo...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00030025
更新日期:1984-09-01 00:00:00
abstract::Pigment-protein-complexes of two chlorophyll b deficient mutants of Arabidopsis and from the wild type were separated electrophoretically. Light-harvesting proteins were absent in the chlorophyll b free mutant ch(1) and their amount was reduced in the mutant ch(2) which has a reduced content of chlorophyll b. The rati...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041827
更新日期:1983-01-01 00:00:00
abstract::Effect of water-soluble and stable sucrose-bound iron oxyhydroxide nanoparticles [Fe[III] sucrose complex (FSC)] on the efficiency of electron transport in the photosystem II membranes was studied. FSC significantly increases (by a factor 1.5) the rate of light-induced oxygen evolution in the presence of alternative e...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-019-00647-4
更新日期:2019-10-01 00:00:00
abstract::Chlorobaculum [Cba.] tepidum is known to grow optimally at 48-52 °C and can also be cultured at ambient temperatures. In this paper, we prepared constant temperature, temperature shift, and temperature shift followed by backshift cultures and investigated the intrinsic properties and spectral features of chlorosomes f...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-013-9800-y
更新日期:2013-05-01 00:00:00
abstract::Using a novel, pulsed micro-second time-resolved photoacoustic (PA) instrument, we measured thermal dissipation and energy storage (ES) in the intact cells of wild type (WT) Chlamydomonas reinhardtii, and mutants lacking either PSI or PSII reaction centers (RCs). On this time scale, the kinetic contributions of the th...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9682-9
更新日期:2011-09-01 00:00:00
abstract::This article gives an historical overview of our group's research on various metabolite translocators of chloroplasts, such as the translocators for phosphorylated intermediates of the Calvin-Benson cycle and of glycolysis, of ADP and ATP, of dicarboxylates, of pyruvate and of hexoses; how it began and where it led to...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1020475803784
更新日期:2002-01-01 00:00:00
abstract::This work addresses the question of occurrence and function of photosystem II (PSII) in bundle sheath (BS) cells of leaves possessing NADP-malic enzyme-type C4 photosynthesis (Zea mays). Although no requirement for PSII activity in the BS has been established, several component proteins of PSII have been detected in B...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-014-0009-5
更新日期:2014-10-01 00:00:00
abstract::The role of photosynthetic pigments in the development of separation methods in biochemistry during the period 1900-1980 is described beginning with M. Tswett who introduced separation of chlorophylls and carotenoids on columns and coined the term chromatography in 1906. In Uppsala, T. Svedberg developed the ultracent...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1024944606930
更新日期:2003-01-01 00:00:00
abstract::Henrik Lundegårdh made major contributions in the field of ecology and plant physiology from 1912 to 1969. His early work at Hallands Väderö in the Kattegat pioneered quantitative approaches to plant ecology and laid the understanding of carbon dioxide exchange in natural communities which is still useful today in glo...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1024980325118
更新日期:2003-01-01 00:00:00
abstract::A combination of inhibitor and (15)N studies were used to investigate the photorespiratory nitrogen cycle in maize, a C4 plant. Inhibitors used included isonicotinyl hydrazide which blocks the conversion of glycine to serine, methionine sulfoximine an inhibitor of GS and azaserine an inhibitor of GOGAT. Results from l...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041796
更新日期:1983-01-01 00:00:00
abstract::The cyanobacterial small CAB-like proteins (SCPs) are one-helix proteins with compelling similarity to the first and third transmembrane helix of proteins belonging to the CAB family of light-harvesting complex proteins in plants. The SCP proteins are transiently expressed at high light intensity and other stress cond...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9244-3
更新日期:2008-02-01 00:00:00
abstract::Lipids are important components of transmembrane protein complexes. In order to study the roles of lipids in photosystem II (PSII), we treated the PSII core dimer complex from a thermophilic cyanobacterium Thermosynechococcus vulcanus with phospholipase A(2) (PLA(2)) and lipase, and examined their effects on PSII stru...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-008-9335-9
更新日期:2008-10-01 00:00:00