Recent progress in the eye irritation test.

Abstract:

:The rabbit eye irritation test based on the Draize method is required for the hazard assessment of chemicals and products that may come into contact with the eye. Due to the potential for the suffering of animals and subjectivity of the test, many modifications of the method have been made that involved a reduction in the number of animals and a refinement of techniques. Additionally, there has been significant development of in vitro alternatives. This paper reviews recent advances in the in vivo test and in vitro alternatives, as well as regulatory requirements. While the refinement of in vivo protocols has resulted in a reduction in the number and discomfort on animals, the development of in vitro alternatives could lead to an eventual replacement of animal studies. In view of the inherent simplicity of many in vitro methods, some of which comprise cell cultures, further research into the relevance/mechanism of effects is required. Batteries of in vitro tests, when properly validated, may be considered as replacements for animal testing.

journal_name

Toxicol Ind Health

authors

Chu IH,Toft P

doi

10.1177/074823379300900602

subject

Has Abstract

pub_date

1993-11-01 00:00:00

pages

1017-25

issue

6

eissn

0748-2337

issn

1477-0393

journal_volume

9

pub_type

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