Abstract:
:The concentration of oxygen within cells is important in many physiological and pathological processes, but such oxygen-dependent phenomena are generally studied as a function of the concentration of extracellular oxygen, due to a lack of suitable methods. Electron parmamagnetic resonance (EPR) oximetric techniques offer an attractive alternative to make such measurements. Previous EPR oximetric studies of extracellular-intracellular oxygen gradients have been hindered, however, by the fact that separate samples, prepared in slightly different ways, were required for individual measurements of extracellular and intracellular oxygen concentrations. In this study we demonstrate a technique that allows simultaneous measurement of intracellular and extracellular oxygen concentrations in a single sample: extracellular measurement is achieved using positively charged nitroxides encapsulated in liposomes, while intracellular oxygen is determined using a membrane-permeable nitroxide along with an extracellular broadening agent. Application of this system to the measurement of oxygen concentrations in suspensions of rat myoblast cells gave results which are consistent with non-simultaneous measurements and which show substantial extracellular-intracellular oxygen gradients in these rapidly respiring cells.
journal_name
Magn Reson Medjournal_title
Magnetic resonance in medicineauthors
Glockner JF,Norby SW,Swartz HMdoi
10.1002/mrm.1910290105subject
Has Abstractpub_date
1993-01-01 00:00:00pages
12-8issue
1eissn
0740-3194issn
1522-2594journal_volume
29pub_type
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