Abstract:
:The transcription of human papillomavirus type 16 (HPV-16) is mediated by the viral enhancer. Epithelial cell-specific activation is achieved by the cooperative interaction of apparently ubiquitous transcriptional factors. One of them, nuclear factor I (NFI), binds seven sites within the HPV-16 enhancer. Point mutations on enhancer fragments, which retain epithelial cell specificity, verify the functional contribution of NFI. In band shift experiments, the epithelial cell-derived NFI proteins CTF-1, CTF-2, and CTF-3 form a characteristic pattern of heterodimeric complexes which are observed in all epithelial cells tested. Divergence from this pattern in fibroblasts, liver cells, and lymphoid cells correlates with the lack of HPV-16 enhancer activation. The HPV-16 enhancer can be activated by CTF-1 in SL-2 cells, which lack NFI-like proteins. However, exogenous CTF-1 fails to overcome the inactivity of the viral enhancer in fibroblasts. Western immunoblot and supershift analysis shows that exogenously introduced CTF-1 proteins form different heterodimer complexes with the given subset of endogenous NFI proteins in epithelial or fibroblast cells. Polymerase chain reaction analysis and cDNA library screens identified the endogenous fibroblast type NFI as NFI-X, an NFI family member originally cloned from hamster liver cells. The strict correlation between the activation or lack of activation of the HPV-16 enhancer and cell-specific subsets of NFI proteins argues for the pivotal role of NFI binding sites in the epithelial cell-specific function of the viral enhancer.
journal_name
J Viroljournal_title
Journal of virologyauthors
Apt D,Chong T,Liu Y,Bernard HUdoi
10.1128/JVI.67.8.4455-4463.1993subject
Has Abstractpub_date
1993-08-01 00:00:00pages
4455-63issue
8eissn
0022-538Xissn
1098-5514journal_volume
67pub_type
杂志文章abstract::The Vpr accessory gene product of human immunodeficiency virus types 1 and 2 and simian immunodeficiency virus is believed to play a role in permitting entry of the viral core into the nucleus of nondividing cells. A second role for Vpr was recently suggested by Rogel et al. (M. E. Rogel, L. I. Wu, and M. Emerman, J. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.6705-6711.1995
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doi:10.1128/JVI.13.5.1046-1054.1974
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.3726-3733.1990
更新日期:1990-08-01 00:00:00
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doi:10.1128/JVI.73.5.3595-3602.1999
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更新日期:2016-04-29 00:00:00
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pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2006-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.7745-7753.1998
更新日期:1998-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01189-18
更新日期:2019-01-04 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.18.8402-8412.2000
更新日期:2000-09-01 00:00:00
abstract::Two defective RNAs (designated D RNA 3 alpha and D RNA 3 beta) were found to be associated with the Fny strain of cucumber mosaic cucumovirus but not with the Sny strain after serial passages in a tobacco host. The D RNAs were derived from RNA 3 by single, in-frame deletions within the 3a open reading frame. A full-le...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.8.4746-4751.1995
更新日期:1995-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02444-09
更新日期:2010-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.1.221-224.1987
更新日期:1987-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.18.9069-9078.2002
更新日期:2002-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00106-07
更新日期:2007-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.00505-06
更新日期:2006-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.32.2.692-696.1979
更新日期:1979-11-01 00:00:00
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00773-17
更新日期:2017-10-27 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.11.5728-5736.2004
更新日期:2004-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02246-15
更新日期:2015-12-04 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4454-4457.1998
更新日期:1998-05-01 00:00:00
abstract::DNA vaccines have recently emerged at the forefront of approaches to harness the immune system in the prevention and treatment of viral infections, as well as the prevention and treatment of cancers. However, these vaccines suffer from limited efficacy since they often fail to produce significant antigen-specific CD8(...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01954-09
更新日期:2010-03-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) infection causes adult T-cell leukemia (ATL), which is frequently resistant to currently available therapies and has a very poor prognosis. To prevent the development of ATL among carriers, it is important to control HTLV-1-infected cells in infected individuals. Therefore, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01885-17
更新日期:2018-01-30 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1818-1821.1989
更新日期:1989-04-01 00:00:00
abstract::HIV circumvents HLA class I-restricted CD8+ T-cell responses through selection of escape mutations that leave characteristic mutational "footprints," also known as HLA-associated polymorphisms (HAPs), on HIV sequences at the population level. While many HLA footprints are universal across HIV subtypes and human popula...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01128-17
更新日期:2018-01-02 00:00:00
abstract::Gammaherpesvirus 68 (gammaHV68, or MHV68) is a naturally occurring rodent pathogen that replicates to high titer in cell culture and is amenable to in vivo experimental evaluation of viral and host determinants of gammaherpesvirus disease. However, the inability of MHV68 to transform primary murine B cells in culture,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02689-07
更新日期:2008-08-01 00:00:00
abstract:UNLABELLED:Despite encoding multiple viral proteins that modulate the retinoblastoma (Rb) protein in a manner classically defined as inactivation, human cytomegalovirus (HCMV) requires the presence of the Rb protein to replicate efficiently. In uninfected cells, Rb controls numerous pathways that the virus also command...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01718-15
更新日期:2015-12-01 00:00:00