Abstract：
:The hypernociceptive role played by the chemokine CCL2, and its main receptor, CCR2, in pathological settings is being increasingly recognized. We aimed to characterize the involvement of spinal CCL2 in the hyperalgesia due to the intratibial inoculation of fibrosarcoma NCTC 2472 cells in mice. The intrathecal (i.t.) administration of the CCR2 antagonist RS 504393 (1–3 μg) or an anti-CCL2 antibody inhibited tumoral hyperalgesia. No change in the expression of spinal CCR2 was detected by western blot, whereas immunohistochemical experiments demonstrated increased CCL2 staining at the superficial laminae of the spinal cord ipsilateral to the tumor. This spinal CCL2 does not seem to be released from nociceptors since CCL2 mRNA and CCL2 levels in DRGs, as measured by RT-PCR and ELISA, remain unmodified in tumor-bearing mice. In contrast, immunohistochemical assays demonstrated the spinal up-regulations of GFAP and Iba-1, respective markers of astroglia and microglia, and the expression of CCL2 in both types of glial cells at the superficial laminae of the spinal cord of tumor-bearing mice. Finally, since CCL2 could induce astroglial or microglial activation, we studied whether the blockade of CCR2 could inhibit the increased spinal glial expression. GFAP, but not Iba-1, up-regulation was reduced in tumor-bearing mice treated for 3 days with i.t. RS 504393, indicating that spinal CCL2 acts as an astroglial activator in this setting. The participation at spinal level of CCL2/CCR2 in tumoral hypernociception, together with its previously described involvement at periphery, makes attractive the modulation of this system for the alleviation of neoplastic pain.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Pevida M,González-Rodríguez S,Lastra A,García-Suárez O,Hidalgo A,Menéndez L,Baamonde Adoi
10.1007/s10571-013-9995-7subject
Has Abstractpub_date
2014-01-01 00:00:00pages
143-56issue
1eissn
0272-4340issn
1573-6830journal_volume
34pub_type
杂志文章abstract：:1. Angiotensin II receptors have been studied by quantitative autoradiography in selected brain areas of young (2-week-old) and adult (8-week-old) rats. 2. In young rats, angiotensin II receptors were present in brain areas which did not express receptors in the adult brain, such as thalamic nuclei, cortical areas, an...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/BF00769042
更新日期：1991-04-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1023/a:1022575126738
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-015-0326-z
更新日期：2016-11-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1023/a:1022997805525
更新日期：2003-04-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-008-9308-8
更新日期：2009-03-01 00:00:00
abstract：:: 1. Glutaric acidemia type I (GA I) is a neurometabolic disorder caused by deficiency of glutaryl-CoA dehydrogenase, which leads to tissue accumulation of predominantly glutaric acid (GA) and also 3-hydroxyglutaric acid to a lesser amount. Affected patients usually present progressive cortical atrophy and acute stria...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-006-9134-9
更新日期：2007-06-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1023/b:cemn.0000018621.58328.ea
更新日期：2004-04-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-019-00683-7
更新日期：2019-08-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-007-9199-0
更新日期：2008-02-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-005-3151-y
更新日期：2005-03-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-004-1378-7
更新日期：2005-02-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/BF00710943
更新日期：1984-03-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-010-9635-4
更新日期：2011-04-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-009-9354-x
更新日期：2009-07-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-009-9485-0
更新日期：2010-05-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-012-9848-9
更新日期：2012-11-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-005-3970-x
更新日期：2005-06-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-015-0172-z
更新日期：2015-08-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-010-9618-5
更新日期：2011-03-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/BF00711249
更新日期：1988-06-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-009-9388-0
更新日期：2009-09-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-019-00654-y
更新日期：2019-03-01 00:00:00
abstract：:Available treatment for Parkinson's disease (PD) is mainly symptomatic instead of halting or reversing degenerative processes affecting the disease. Research on the molecular pathogenesis of PD has suggested reduced trophic support as a possible cause or mediator of neurodegeneration. In animal models of the disease, ...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-009-9458-3
更新日期：2010-04-01 00:00:00
abstract：:Amyotrophic lateral sclerosis is a devastating motoneuron disorder for which no effective treatment exists. There is some evidence for neuroprotective effects of valproic acid (VPA). The beneficial effects, however, are limited due to the adverse effects of VPA. To overcome this problem, a number of VPA derivates with...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-010-9518-8
更新日期：2010-08-01 00:00:00
abstract：:1. A lambda phage genomic DNA library for Limulus (L.) polyphemus brain was constructed using the AGEM-12 vector and the host strain KW251. 2. The primary library contained approximately 1.275 x 10(6) independent clones, increasing upon amplfication to 6.66 x 10(9) pfu/ml in a total volume of 58 ml. 3. A total of 28 c...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1023/a:1007027232412
更新日期：2000-08-01 00:00:00
abstract：:Diabetes damages the central nervous system, inducing cognitive dysfunction and structural changes, known as diabetic encephalopathy (DE). Some research suggests that the pathogenesis of DE may involve an inflammatory imbalance in the nervous system, along with β-amyloid deposition, similar to Alzheimer's disease. Les...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-015-0209-3
更新日期：2015-11-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1007/s10571-017-0547-4
更新日期：2018-01-01 00:00:00
abstract：:1. It has been discussed for over 100 years whether short-term memory (STM) is separate from, or just an early phase of, long-term memory (LTM). The only way to solve this dilemma is to find out at least one treatment that blocks STM while keeping LTM intact for the same task in the same animal. 2. The effect of a lar...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1023/a:1020715800956
更新日期：2002-06-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1023/a:1019865822069
更新日期：2002-04-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-006-9067-3
更新日期：2006-10-01 00:00:00
