Abstract：
:The field of study concerning promotion and/or inhibition of angiogenesis has gathered much attention in the scientific community. A great deal of work has been invested towards defining reproducible assays to gauge for promotion or inhibition of angiogenesis in response to drug treatments or growth conditions. Two common components of these assays were noted by our group to have an unexpected and previously unreported interaction. Suramin is a commercially available compound, commonly used as a positive control for in vitro angiogenic inhibition assays. Matrigel is a popular extracellular substrate that supports angiogenic network formation when endothelial cells are cultured on its surface. However, our group demonstrated that suramin alone (without the presence of cells) will actively dissolve Matrigel, causing the extracellular matrix to transition from the gel-like physical state to a more liquid state. This causes cells on the Matrigel to congregate and sink to the bottom of the well. Therefore, previous observations of inhibition of endothelial cell angiogenesis through the incubation with suramin (including previous observations made by our group) are, largely, an artefact caused by suramin and matrix interaction rather than suramin and cells interaction, as previously reported. Our results suggest that the presence of sulphate groups and amphiphilic properties of suramin are likely responsible for the disruption of the matrix layer. We believe that this information is of prime importance to anyone using similar in vitro models, or employing suramin in any therapy or drug development assays.
journal_name
Int J Exp Patholjournal_title
International journal of experimental pathologyauthors
Prigozhina NL,Heisel AJ,Seldeen JR,Cosford ND,Price JHdoi
10.1111/iep.12043subject
Has Abstractpub_date
2013-12-01 00:00:00pages
412-7issue
6eissn
0959-9673issn
1365-2613journal_volume
94pub_type
杂志文章abstract：:The aim of this study was to evaluate the use of flaxseed in animals subjected to ethanol-induced hepatotoxicity. Twenty-four male rats were divided into four groups (n = 6): control group (CG) which received a control diet and water ad libitum; flaxseed group (FG) which received control diet with an addition of 25% f...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12162
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doi：10.1111/j.1365-2613.2007.00559.x
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2011.00762.x
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.2003.00336.x
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journal_title：International journal of experimental pathology
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更新日期：1994-08-01 00:00:00
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.1998.00055.x
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
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journal_title：International journal of experimental pathology
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doi：
更新日期：1990-10-01 00:00:00
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journal_title：International journal of experimental pathology
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更新日期：1994-12-01 00:00:00
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
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