Abstract：
:A recent global gene expression profiling study unexpectedly showed that activated oncogenic NRAS may recruit neural precursor cell expressed, developmentally downregulated 4L (Nedd4L; a human homologue of Nedd4-2) in cultured melanoma cells. However, whether Nedd4L was expressed in melanoma tissues or participated in melanoma carcinogenesis remains to be clarified. Here, we investigated the expression status of Nedd4L in human melanocytes, benign nevi and melanoma tissue specimens and subsequently attempted to determine the role of Nedd4L in melanoma cell growth. Immunohistochemical staining revealed that Nedd4L was not present in any non-tumorous melanocytes or in 18 benign nevi tissues, but it was detected in 34 of 79 cutaneous melanomas and 9 of 32 nodal metastatic melanomas. Downregulation of Nedd4L significantly reduced the growth of cultured G361 melanoma cells in vitro. Moreover, exogenous Nedd4L expression significantly promoted the growth of A2058 melanoma cells in vivo in a xenograft assay. The present findings indicate that Nedd4L expression may be increased to facilitate tumour growth in many melanomas.
journal_name
Int J Exp Patholjournal_title
International journal of experimental pathologyauthors
Kito Y,Bai J,Goto N,Okubo H,Adachi Y,Nagayama T,Takeuchi Tdoi
10.1111/iep.12051subject
Has Abstractpub_date
2014-02-01 00:00:00pages
24-8issue
1eissn
0959-9673issn
1365-2613journal_volume
95pub_type
杂志文章abstract：:The degenerative and regenerative changes in murine skeletal muscle after injection of Bothrops asper venom were studied by histological, lectin histochemical and immunocytochemical techniques. According to our observations, the process was divided into four main stages: (a) During the first 3 days prominent degenerat...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:An important means of determining how amyloid-beta peptide (Abeta) affects cells is to identify specific macromolecular targets and assess how Abeta interaction with such targets impacts on cellular functions. On the one hand, cell surface receptors interacting with extracellular Abeta have been identified, and their ...
journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
doi：10.1111/j.0959-9673.2005.00427.x
更新日期：2005-06-01 00:00:00
abstract：:The constitutive cell types of the two lung cancer cell lines CaLu1 and CaLu3 have been investigated by immunocytochemical staining with markers for cytokeratins, vimentin, carcinoembryonic antigen and the epithelial cell epitope recognized by the monoclonal antibody Ber-EP4. The cells of both lines reacted with vimen...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
abstract：:Systemic hypertension, characterized by elevated blood pressure ≥140/90 mm Hg, is a major modifiable risk factor for cardiovascular disease. Hypertension also associates with non-alcoholic fatty liver disease (NAFLD), which is becoming common due to a modern diet and lifestyle. The aim of the present study was to exam...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12369
更新日期：2020-10-01 00:00:00
abstract：:Tumour growth is angiogenesis dependent. Thrombosis and thromboembolism are very common in cancer patients. These patients are often treated with heparin as an anti-coagulant. Many tumour angiogens, including VEGF165, and endogenous anti-angiogenesis factors bind heparin tightly. Using the non-surgical mesenteric-wind...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.2000.00150.x
更新日期：2000-06-01 00:00:00
abstract：:Progress in understanding the pathophysiology of abdominal aortic aneurysms (AAA) is dependent in part on the development and application of effective animal models that recapitulate key aspects of the disease. The objective was to produce an experimental model of AAA in rats by combining two potential causes of metal...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2010.00746.x
更新日期：2011-02-01 00:00:00
abstract：:In this study, viruria following Japanese encephalitis virus (JEV) infection in mice has been shown to appear earlier in pregnant than in normal mice with proteinuria and haematuria. This was related to the production of splenic macrophage derived neutrophil chemotactic factor (MDF) following JEV infection. Intravenou...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:There is evidence to show that downregulation of miR-1 expression is closely related to cancer progression, including in nasopharyngeal carcinoma (NPC). However, the molecular mechanisms underlying miR-1 downregulation in NPC remain largely unknown, especially its association with Epstein-Barr virus (EBV). In this stu...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12162
更新日期：2015-12-01 00:00:00
abstract：:Initially found expressed in neuronal and then later in endothelial cells, it is well established that the transmembrane glycoproteins neuropilin-1 (NRP1) and neuropilin-2 (NRP2) play essential roles in axonal growth and guidance and in physiological and pathological angiogenesis. Neuropilin expression and function in...
journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2613.2012.00810.x
更新日期：2012-04-01 00:00:00
abstract：:Skin window procedures in humans have shown rapid accumulation of neutrophils into the exuded fluids above abraded skin. The present study was undertaken to determine if similar epicutaneous neutrophil accumulation might explain the extreme resistance of HRS/J mice, both hairless (hr/hr) and haired (hr/+), to experime...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2008.00584.x
更新日期：2008-06-01 00:00:00
abstract：:The field of study concerning promotion and/or inhibition of angiogenesis has gathered much attention in the scientific community. A great deal of work has been invested towards defining reproducible assays to gauge for promotion or inhibition of angiogenesis in response to drug treatments or growth conditions. Two co...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12043
更新日期：2013-12-01 00:00:00
abstract：:Several methods have been described for investigating chondrocyte metabolism in vitro. In this study, in-situ hybridization (ISH) using an oligonucleotide probe (i.e. a poly-d(T) probe) to detect total messenger RNA (mRNA) in cartilage explants has been compared with radiosulphate and radioleucine uptake studies in an...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:The purpose of this study was to examine the prognostic significance of insulin-like growth factor-1 receptor (IGF-1R) expression alone and in relation to the expression of the MET- receptor and the MET-homologous receptor RON, in classical Hodgkin's lymphoma (cHL). Tumour samples from patients with cHL (n = 202; medi...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12128
更新日期：2015-08-01 00:00:00
abstract：:Blister formation and skin damage can be induced by BaP1, a haemorrhagic metalloproteinase from the venom of the snake Bothrops asper. Pathological changes in the skin were investigated after intramuscular injections of Bothrops asper haemorrhagic metalloproteinase BaP1. Blisters developed within the first hour, with ...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1998-08-01 00:00:00
abstract：:Intratracheal instillation (IT) of bleomycin is a widely used experimental model for lung fibrosis. In this study we describe the time-course of bleomycin-induced lung fibrosis in mice using computer-assisted morphometry. C57Bl/6J mice were treated with a single IT dose of bleomycin or control saline. Animals were kil...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.2002.00220.x
更新日期：2002-06-01 00:00:00
abstract：:The relationship between donor age and the growth characteristics of normal human smooth muscle cell cultures derived from aorta and stomach was examined in a series of growth studies. A negative correlation was demonstrated between donor age and growth potential of both cell types in primary culture. This decreased g...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:The Fell-Muir Award requires the recipient to deliver a lecture and a review manuscript which provides a personal overview of significant scientific developments in the field of matrix biology over the period of the recipient's career. In this context, this review considers the collagen family of structural proteins a...
journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2613.2007.00537.x
更新日期：2007-08-01 00:00:00
abstract：:Formation of the coronary arteries consists of a precisely orchestrated series of morphogenetic and molecular events which can be divided into three distinct processes: vasculogenesis, angiogenesis and arteriogenesis (Risau 1997; Carmeliet 2000). Even subtle perturbations in this process may lead to congenital coronar...
journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2613.2009.00646.x
更新日期：2009-06-01 00:00:00
abstract：:Haptoglobin is an acute phase protein known to be highly expressed in the liver. Recently, we showed increased local arterial haptoglobin expression after flow-induced arterial remodelling and found that haptoglobin is involved in cell migration and arterial restructuring probably through accumulation of a temporary g...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.2003.00336.x
更新日期：2003-04-01 00:00:00
abstract：:The present study was carried out to investigate the role of hypertension in the genesis and localization of intimal lesions and medial remodelling found in the prestenotic segment in relation to a severe stenosis of the abdominal aorta just below the diaphragm. Male young rats were divided randomly into operated grou...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2006.00506.x
更新日期：2006-12-01 00:00:00
abstract：:Previously we have found deregulation of collagen metabolism in human pancreatitis and pancreatic cancer tissues. Insulin-like growth factor-I (IGF-I) is known to stimulate collagen biosynthesis through interaction with IGF-I receptor. IGF-I binding proteins (BPs) regulate the activity of IGF-I. We investigated whethe...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.2002.00237.x
更新日期：2002-10-01 00:00:00
abstract：:Diets enriched with fat, especially unsaturated fat, promote experimental pancreatic carcinogenesis, but little is known of the effects of individual fatty acids. The effect of stearic and oleic acid on pancreatic fatty acids and atypical acinar cell nodules (preneoplastic lesions) was studied in 14-day-old weanling m...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:To confirm the existence of the cervical thymus and the development of cervical thymoma in thymoma-prone BUF/Mna (BUF) rats, we examined cervical organs and adjacent tissues, and thoracic thymic tissues of the three inbred strains, BUF, ACI/NMna (ACI), and WKY/NCrj (WKY), and 11 congenic strains, in which genetic regi...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2010.00755.x
更新日期：2011-04-01 00:00:00
abstract：:The Meriones unguiculatus (Mongolian) gerbil has demonstrated significant prostatic responses to hormonal treatments, and to drugs against human prostatic hyperplasia. Spontaneous neoplasia develops in the older animals. Thirty gerbils (age 18 months) were divided into non-affected and prostatic lesion bearers and the...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2007.00550.x
更新日期：2008-02-01 00:00:00
abstract：:The aim of the present study was to evaluate the coexpression of very early (CD69), early (CD25) and late (HLADR) antigens and to analyse the mean fluorescence intensity (MFI) of such activation markers on synovial fluid (SF) and peripheral blood (PB) lymphocytes of patients affected by rheumatoid arthritis (RA) and o...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.1997.290360.x
更新日期：1997-10-01 00:00:00
abstract：:Permethrin (PM), a synthetic pyrethroid insecticide, has broad toxicity spectra. We aimed to investigate the effects of PM on the testes of adult albino rats, examine the recovery response and evaluate the efficacy of naringenin (NG) supplementation. Adult male albino rats were randomly assigned to five groups of six ...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12168
更新日期：2016-02-01 00:00:00
abstract：:Ureteropelvic junction (UPJ) obstruction is the most frequently observed cause of obstructive nephropathy in children. Neonatal and foetal animal models have been developed that mimic closely what is observed in human disease. The purpose of this review is to discuss how obstructive nephropathy alters kidney histology...
journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2613.2010.00727.x
更新日期：2011-06-01 00:00:00
abstract：:The interrelationship between acinar cell apoptosis and tubular complex formation was examined in caerulein-induced pancreatitis using histology, immunohistochemistry, electron microscopy and DNA gel electrophoresis. Rats were given 8 hourly subcutaneous injections of caerulein, 24 micrograms/kg, for up to 2 days. Mor...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.1999.00116.x
更新日期：1999-08-01 00:00:00
abstract：:An autoimmune kidney disease morphologically and functionally similar to Heymann nephritis (HN) was induced in mature male Sprague Dawley rats by repeated weekly IP injections of a chemically modified azo sonicated ultracentrifuged (u/c) rat kidney fraction 3 (rKF3) antigen in an aqueous medium. The experiment was ter...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.0959-9673.2004.00389.x
更新日期：2004-10-01 00:00:00
abstract：:Interstitial fibrosis, associated with extensive accumulation of extracellular matrix constituents in the cortical interstitium, is directly correlated to progression of renal disease. The earliest histological marker of this progression is the accumulation in the interstitium of fibroblasts with the phenotypic appear...
journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2613.2011.00764.x
更新日期：2011-06-01 00:00:00
