Comparative antigen-induced gene expression profiles unveil novel aspects of susceptibility/resistance to adjuvant arthritis in rats.

abstract：:Hybridomas producing anti-histone monoclonal antibodies were generated from a 2-month old MRL lpr/lpr mouse. Five IgM antibodies showed binding to histone H3 and three of these IgM antibodies also bound histone H1. Two IgG2a antibodies (MRA3 and MRA12) were specific for histone H1 and their binding was further charact...

journal_title：Molecular immunology

authors： Monestier M,Fasy TM,Böhm L

更新日期：1989-08-01 00:00:00

abstract：:We investigated the antibody (Ab) and cytotoxic T lymphocyte (CTL) responses to gene gun (GG)-mediated DNA immunization via the intramuscular (i.m.) and intradermal (i.d.) routes. BALB/c mice were immunized five times at weekly intervals with plasmid DNA encoding enhanced green fluorescent protein (EGFP). EGFP product...

journal_title：Molecular immunology

authors： Ito K,Ito K,Shinohara N,Kato S

更新日期：2003-05-01 00:00:00

abstract：:alpha 2-Macroglobulin (alpha 2M) complexed with proteinases or modified by the action of amines has been shown to affect immune responses in vitro, though as yet the mechanisms are poorly understood. Supernates from rabbit lymphoid cells cultured in medium with normal rabbit serum and 35S-methionine (or 14C-leucine) w...

journal_title：Molecular immunology

authors： Schlesinger C,McEntire J,Wallman J,Skosey JL,Hanly WC,Teodorescu M

更新日期：1989-03-01 00:00:00

abstract：:T-lymphocyte proliferative responses were determined with peripheral blood lymphocytes (PBL), peritoneal exudate lymphocytes (PEL) and lymph node lymphocytes (LNL) of guinea pigs immunized with horse cytochrome c (HCytc). The proliferative response of PBL activated by peptide 81-104 from animals immunized with HCytc w...

journal_title：Molecular immunology

authors： Wang KM,Reichlin M

更新日期：1988-08-01 00:00:00

abstract：:The objective of this study was to characterize the in vitro and in vivo activity of a novel afucosylated rituximab (BLX-300) expressed in a Lemna aquatic plant-based system free of zoonotic pathogens. The glycosylation of BLX-300 was shown to be homogeneous, composed of a single major N-glycan species without detecta...

journal_title：Molecular immunology

authors： Gasdaska JR,Sherwood S,Regan JT,Dickey LF

更新日期：2012-03-01 00:00:00

abstract：:the SC5b-8 complex of human complement is a hydrophilic molecule of mol. wt 800,000-850,000 that is seen in the electron microscope as an elongated, straight or curved structure of 50-55 nm total length and 8-9 nm width. Tryptic attack on the fluid-phase complex exposes lipid-binding surfaces on the molecule. The tryp...

journal_title：Molecular immunology

authors： Bhakdi S,Tranum-Jensen J

更新日期：1982-09-01 00:00:00

abstract：:1,25-Dihydroxyvitamin D3 (1,25-D3) is known to have potent inhibitory effects on human peripheral blood mononuclear cell (PBMC) functions. Previous experiments suggest that addition of interleukin-2 (IL-2) to cell cultures can reverse the antiproliferative action of 1,25-D3. Previous studies have also shown that the C...

journal_title：Molecular immunology

authors： Jordan SC,Lemire JM,Sakai RS,Toyoda M,Adams JS

更新日期：1990-01-01 00:00:00

abstract：:The sphingosine-1-phosphate analogue FTY720 is known to alter migration and homing of lymphocytes via sphingosine-1-phosphate receptors. However, several studies indicate that its mode of action is more complex and that FTY720 may also directly influence cytokine effector functions. Therefore, we studied the effect of...

journal_title：Molecular immunology

authors： Daniel C,Sartory NA,Zahn N,Schmidt R,Geisslinger G,Radeke HH,Stein JM

更新日期：2007-07-01 00:00:00

abstract：BACKGROUND:Allergen epitope characterization provides valuable information useful for the understanding of proteins as food allergens. It is believed that IgE epitopes in general are conformational, nevertheless, for food allergens known to sensitize through the gastrointestinal tract linear epitopes have been suggeste...

journal_title：Molecular immunology

authors： Bøgh KL,Nielsen H,Madsen CB,Mills EN,Rigby N,Eiwegger T,Szépfalusi Z,Roggen EL

更新日期：2012-07-01 00:00:00

abstract：:The mammalian interleukin (IL)-6 family of cytokines consist of eight class-I helical cytokines and are major players in hematopoiesis, as well as in neuroendocrine and immune systems, and have pro- and anti-inflammatory properties. We have identified a novel fish CNTF-like molecule, for the first time, as well as the...

journal_title：Molecular immunology

authors： Wang T,Secombes CJ

更新日期：2009-07-01 00:00:00

abstract：:When human serum is diluted and pre-incubated at 37 degrees C in low ionic strength buffer (LIS, u = 0.07; made iso-osmotic with dextrose), a spontaneous activation of complement (C) is observed as determined by C4 and C3 electrophoretic conversion. In this paper it is postulated that most species of glycosaminoglycan...

journal_title：Molecular immunology

authors： Chang NS,Boackle RJ

更新日期：1986-08-01 00:00:00

abstract：:We discovered that some bovine antibodies are amongst the largest known to exist due to the presence of an exceptionally long CDR3H (≥49 amino acids) with multiple cysteines that provide a unique knob and stalk structure to the antigen binding site. The large CDR3H size, unlike mouse and human, provides a suitable pla...

journal_title：Molecular immunology

authors： Pasman Y,Soliman C,Ramsland PA,Kaushik AK

更新日期：2016-09-01 00:00:00

abstract：:The molecular interactions between the components of the C3 convertase of the alternative pathway (AP) of complement and its regulators, in both surface-bound and fluid-phase form, are still incompletely understood. The fact that the AP convertase is labile makes studies difficult to perform. According to the so calle...

journal_title：Molecular immunology

authors： Bexborn F,Andersson PO,Chen H,Nilsson B,Ekdahl KN

更新日期：2008-04-01 00:00:00

abstract：:Peptide fragments of pertussis toxin subunit 1 (PT-S1) have been synthesized in order to investigate their antigenic and immunogenic activity, and to evaluate their possible use as components of a new vaccine. Two peptides (sequence 73-82, EAERAGRGTG and sequence 107-116, YVDTYGDNAG) were selected for their predictabl...

journal_title：Molecular immunology

authors： Presentini R,Perin F,Ancilli G,Nucci D,Bartoloni A,Rappuoli R,Antoni G

更新日期：1989-01-01 00:00:00

abstract：:A relatively large number of variable region genes (V) contribute, via gene rearrangements with smaller numbers of additional gene elements (D and J), to generate diversity in the immune response. While some VH gene families are thought to contain 100- 1000 members, the VH10 family has only two known functioning membe...

journal_title：Molecular immunology

authors： Chao M,Voss EW Jr

更新日期：1992-03-01 00:00:00

abstract：:MiRNAs are a class of endogenous tiny RNAs that act as inhibitors of translation or promote RNA degradation by duplex-formation within the 3'-UTR of target mRNAs. They play an important role during a wide range of cellular processes by fine-tuning of gene expression. The differentiation of monocytes to macrophages pla...

journal_title：Molecular immunology

authors： Poitz DM,Augstein A,Gradehand C,Ende G,Schmeisser A,Strasser RH

更新日期：2013-12-01 00:00:00

abstract：:Light chains of 426 randomly selected myelomas from BALB/c and NZB mice were characterized by isoelectric focusing (IF) and electrophoresis at pH 3. Approximately 50% of the light chains in each strain were found to have unique electrophoretic properties. The remaining 50% of light chains fell into groups of two or mo...

journal_title：Molecular immunology

authors： Gibson DM

更新日期：1984-05-01 00:00:00

abstract：:We have recently developed an in vitro culture model enabling the large-scale expansion of switched-memory B lymphocytes, producing a polyclonal human IgG repertoire. Given the importance of glycosylation for the functions of immunoglobulins, we analyzed the N-glycosylation profiles of the immunoglobulin G (IgG) in th...

journal_title：Molecular immunology

authors： Ritamo I,Cloutier M,Valmu L,Néron S,Räbinä J

更新日期：2014-02-01 00:00:00

abstract：:Mouse monoclonal antibodies (MAbs) have been prepared against rat T cell blasts. One MAb called MRC OX-40 recognized an antigen that differed from any previously described in that its expression was detected only on T blasts that also expressed the CD4 antigen. The OX-40 MAb did not detect an activation determinant of...

journal_title：Molecular immunology

authors： Paterson DJ,Jefferies WA,Green JR,Brandon MR,Corthesy P,Puklavec M,Williams AF

更新日期：1987-12-01 00:00:00

abstract：:A microchemical assay for phosphorus was applied to the measurement of DNA in immune complexes formed with monoclonal or serum anti-DNA autoantibodies and DNA of varying size and conformation. Two monoclonal antibodies were produced by hybridomas derived from spleen cells of autoimmune MRL-lpr/lpr mice and were purifi...

journal_title：Molecular immunology

authors： Ali R,Dersimonian H,Stollar BD

更新日期：1985-12-01 00:00:00

abstract：:T-cell-replacing factor (TRF)/IL-5 is a T-cell-derived glycoprotein which has pleiotropic activity on lymphoid and myeloid cells. IL-5 polypeptide translated into Xenopus oocytes are heterogeneous in molecular size (40,000 to 60,000 under nonreducing conditions) and yields a monomeric form (Mr of 25,000 to 30,000) und...

journal_title：Molecular immunology

authors： Takahashi T,Yamaguchi N,Mita S,Yamaguchi Y,Suda T,Tominaga A,Kikuchi Y,Miura Y,Takatsu K

更新日期：1990-09-01 00:00:00

abstract：:Endogenous mouse mammary tumor proviruses (MMTV; Mtv loci) deletes Vbeta6 expressing T cells in the thymus of Mtv-7(+) DBA/2 (H2(d)) mice through negative selection. We found that in Mtv-7(-) BALB/c (H2(d)) mice, Vbeta6 is a dominant V gene used in T cell responses to an 18 amino acid long peptide antigen: EYKEYAEYAEY...

journal_title：Molecular immunology

authors： Kilgannon P,Novak Z,Fotedar A,Singh B

更新日期：2010-02-01 00:00:00

abstract：:Polyclonal or monoclonal human IgM rheumatoid factors (RF) react with eight antigenic sites on the CH3 IgG domain, four sites on CH2 and two on human beta 2-microglobulin. All 14 of these RF-reactive epitopes are linear 7-11 amino acid peptides with different primary sequence. We questioned whether RF reactivity with ...

journal_title：Molecular immunology

authors： Williams RC Jr,Malone CC,Kolaskar AS,Kulkarni-Kale U

更新日期：1997-05-01 00:00:00

abstract：:Human beta defensin-1 (hBD1) is a component of the immune system which links the innate and adaptive immune responses. We have demonstrated that hBD1 induces rapid cytolysis of prostate cancer cells and that it may also possess tumor suppressive abilities. In addition, there is a high frequency of cancer-specific loss...

journal_title：Molecular immunology

authors： Bose SK,Gibson W,Bullard RS,Donald CD

更新日期：2009-03-01 00:00:00

abstract：:The binding of a T cell-presented peptide to MHC class II alpha,beta chains occurs as a concurrent process with the release of the associated invariant chain (Ii) by cathepsin B. Ii was digested by cathepsin B from solubilized, MHC class II alpha,beta,Ii complexes in the presence of N-hydroxysuccinimidyl-4-azidobenzoa...

journal_title：Molecular immunology

authors： Daibata M,Xu M,Humphreys RE,Reyes VE

更新日期：1994-03-01 00:00:00

abstract：BACKGROUND:Complement component C5-derived C5a locally generated in the brain has been shown to protect against glutamate-induced neuronal apoptosis and beta-amyloid (Aβ) toxicity, but the mechanism is not clear. In this study, we tested the hypothesis that C5a influences upstream signal transduction pathways associate...

journal_title：Molecular immunology

authors： Gong B,Pan Y,Zhao W,Knable L,Vempati P,Begum S,Ho L,Wang J,Yemul S,Barnum S,Bilski A,Gong BY,Pasinetti GM

更新日期：2013-12-01 00:00:00

abstract：:This study was undertaken to investigate the nature and microheterogeneity of the carbohydrate moiety of the Fc epsilon receptors of RBL-CA10 and RBL-CA10.7 cells. Treatment using the glycosylation processing inhibitors, castanospermine (CN), 1-deoxymannojirimycin (DMJ), and swainsonine (SW) resulted in a decrease of ...

journal_title：Molecular immunology

authors： LaCroix EL,Froese A

更新日期：1993-02-01 00:00:00

abstract：:Phosphorothioate oligonucleotides (S-oligos) are nucleic acid derivatives that are commonly used as antisense agents. These compounds, similar to bacterial DNA and CpG oligonucleotides, display a variety of immunological activities in vitro and in vivo. To assess further these activities, the antigenicity of a series ...

journal_title：Molecular immunology

authors： Pisetsky DS,Reich CF 3rd

更新日期：1998-12-01 00:00:00

abstract：:Proteus sp. and Klebsiella sp. mainly cause infections of the urinary and respiratory tracts or wounds in humans. The representatives of both genera produce virulence factors like lipopolysaccharide (LPS) or outer membrane proteins (OMPs) having much in common in the structures and/or functions. To check how far this ...

journal_title：Molecular immunology

authors： Palusiak A

更新日期：2015-03-01 00:00:00

abstract：:Ligation of the B cell Ag receptor (BCR) activates a protein-tyrosine kinase (PTK) and CD45 protein-tyrosine phosphatase (PTPase)-dependent signaling cascade that results in the activation of Ras. This pathway of Ras activation can operate independently of protein kinase C (PKC) activity. Activation of Ras may lead to...

journal_title：Molecular immunology

authors： Kawauchi K,Lazarus AH,Sanghera JS,Man GL,Pelech SL,Delovitch TL

更新日期：1996-02-01 00:00:00