Abstract:
:Centromere protein F (CENPF) is an essential nuclear protein associated with the centromere-kinetochore complex and plays a critical role in chromosome segregation during mitosis. Up-regulation of CENPF expression has previously been detected in several solid tumors. In this study, we aim to study the expression and functional role of CENPF in hepatocellular carcinoma (HCC). We found CENPF was frequently overexpressed in HCC as compared with non-tumor tissue. Up-regulated CENPF expression in HCC was positively correlated with serum AFP, venous invasion, advanced differentiation stage and a shorter overall survival. Cox regression analysis found that overexpression of CENPF was an independent prognosis factor in HCC. Functional studies found that silencing CENPF could decrease the ability of the cells to proliferate, form colonies and induce tumor formation in nude mice. Silencing CENPF also resulted in the cell cycle arrest at G2/M checkpoint by down-regulating cell cycle proteins cdc2 and cyclin B1. Our data suggest that CENPF is frequently overexpressed in HCC and plays a critical role in driving HCC tumorigenesis.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Dai Y,Liu L,Zeng T,Zhu YH,Li J,Chen L,Li Y,Yuan YF,Ma S,Guan XYdoi
10.1016/j.bbrc.2013.06.021keywords:
["BUB1","CENPF","CHD1L","DAB","Diaminobenzidine","G2\/M transition","HCC","HE","IHC","JTB","MAD1","MAD2","Prognosis factor","SHC1","Src homology 2 domain containing transforming protein 1","TMA","centromere protein F","chromodomain helicase DNA binding protein 1-like","hematoxylin and eosin","hepatocellular carcinoma","immunohistochemistry","jumping translocation breakpoint","mitotic arrest deficient-like 1","mitotic arrest deficient-like 2","mitotic checkpoint serine\/threonine kinase","qRT-PCR","quantitative real time polymerase chain reaction","siRNAs","small interfering RNAs","tissue microarray"]subject
Has Abstractpub_date
2013-07-12 00:00:00pages
711-8issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(13)00995-9journal_volume
436pub_type
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