Abstract:
BACKGROUND:During allograft rejection, up-regulation of cytokine-inducible nitric oxide synthase (iNOS) leads to the production of large amounts of nitric oxide (NO). The net effect of NO on the alloimmune response is, however, difficult to predict because of its diverse biological effects, which include potentially opposing roles as an effector and immunoregulatory molecule. METHODS:In this study, the role of iNOS on the in vitro and in vivo alloimmune response was defined using mutant mice that lack a functional iNOS gene. The ability of spleen cells obtained from iNOS-deficient mutants to proliferate and to produce cytokines in response to irradiated BALB/c stimulator cells was determined, and the rate at which iNOS-deficient mice were able to reject BALB/c skin allografts was observed. RESULTS:Spleen cells from homozygous iNOS-deficient (129xMF1)F1 mice, when compared with cells from heterozygous control mice, showed an increased in vitro proliferative response and produced substantially higher levels of interferon-gamma, and also more interleukin-2 and interleukin-12, in response to allogeneic stimulation. The kinetics of BALB/c skin graft rejection were comparable in heterozygous control animals and iNOS-deficient mice. Moreover, no net effect of iNOS on skin allograft rejection was apparent in mice treated with depleting monoclonal antibodies (mAb) to CD4 or CD8 T cells, either alone or in combination, or in mice treated with both anti-CD8 mAb and a neutralizing anti-tumor necrosis factor mAb. CONCLUSIONS:These results show that iNOS has an immunomodulatory effect on the in vitro alloimmune response but lack of iNOS has no net influence on the kinetics of murine skin allograft rejection in either unmodified recipients or recipients in which the early contribution of T-cell subsets and tumor necrosis factor-alpha to graft rejection has been abrogated.
journal_name
Transplantationjournal_title
Transplantationauthors
Casey JJ,Wei XQ,Orr DJ,Gracie JA,Huang FP,Bolton EM,Liew FY,Bradley JAdoi
10.1097/00007890-199708270-00007subject
Has Abstractpub_date
1997-08-27 00:00:00pages
589-93issue
4eissn
0041-1337issn
1534-6080journal_volume
64pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:The UK Kidney Fast-Track Scheme (KFTS) was introduced in 2012 to identify kidneys at high risk of discard and to rapidly facilitate transplantation. A retrospective analysis of kidneys transplanted through the KFTS was undertaken. METHODS:UK Transplant Registry data were collected on deceased donor kidneys ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001860
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:Conversion from tacrolimus to cyclosporin has not previously been reported as routine clinical practice, but only as indicated by rejection or adverse effects. METHODS:The safety and metabolic outcome of elective conversion from tacrolimus to cyclosporin was examined in 19 recipients of cadaver renal transp...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:2000-07-15 00:00:00
abstract::The effect of cyclosporine and metabolite 17 (M17) as well as other CsA-related compounds (CsG, dihydro-CsC, dihydro-CsD, CsH, B5.49, and H7.94) was tested on T lymphocyte clone proliferation. In these experiments, antigen and interleukin 2 (IL-2) dependent long-term T lymphocyte clones derived from a rejected human k...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198712000-00019
更新日期:1987-12-01 00:00:00
abstract:BACKGROUND:Donation after cardiac death (DCD) increases the number of donor kidneys but is associated with more primary nonfunction (PNF) and delayed graft function (DGF). It has been suggested that biomarkers in the preservation solution of machine perfused kidneys may predict PNF, although evidence is lacking. METHO...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31827908e6
更新日期:2013-02-27 00:00:00
abstract:BACKGROUND:Delayed xenograft rejection (DXR) remains a roadblock to successful xenotransplantation. A feature of DXR is early recruitment of monocytes to the xenograft. Naïve human monocytes can recognize and adhere to unstimulated porcine aortic endothelial cells (PAEC) more than human aortic endothelial cells, partly...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e318191e6b4
更新日期:2009-01-15 00:00:00
abstract:BACKGROUND:Extracorporeal membrane oxygenation (ECMO) and mechanical ventilation (MV) can be used as a bridge to heart-lung transplantation (HLT). The goal of this study was to determine if pretransplantation ECMO or MV affects survival in HLT. METHODS:The United Network for Organ Sharing database was reviewed for all...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3182a860b8
更新日期:2014-01-15 00:00:00
abstract::The recipients of combined kidney-pancreas transplants (SPK) are unique because they routinely receive two allografts from the same donor. In a previous study, we found that the long-term graft survival of the two allografts was different, with better graft survival seen in the pancreas allograft. In an attempt to und...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199408270-00006
更新日期:1994-08-27 00:00:00
abstract:BACKGROUND:Intracellular concentration of reactive oxygen species is held within tight physiological limits by enzymes with scavenging and repair functions. Under extreme conditions such as prolonged cold ischemia, these enzymes may be unable to adequately protect the organ, resulting in reperfusion injury that renders...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200209270-00013
更新日期:2002-09-27 00:00:00
abstract:BACKGROUND:The induction of tolerance to pig antigens in primates may facilitate the development of successful clinical xenotransplantation protocols. The infusion of mobilized porcine peripheral blood leukocytes (PBPCs, comprised of approximately 2% peripheral blood progenitor cells) into splenectomized preconditioned...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200110150-00021
更新日期:2001-10-15 00:00:00
abstract::A rapid microcytotoxicity assay for the detection of HL-A antigens on tissue culture cells derived from human solid tumors is described. Tumor cells were prelabeled with 125Iododeoxyuridine. Isotopically labeled tumor cells were reacted with up to 37 highly selected HL-A antisera and diluted rabbit complement. Results...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197509000-00005
更新日期:1975-09-01 00:00:00
abstract::Erythropoietin (EPO) is widely used among patients with end-stage renal disease awaiting transplantation. Data suggest that EPO therapy may be immunomodulatory. The purpose of this study was to assess the effects of pretransplant EPO therapy on renal allograft outcome. We evaluated 120 consecutive renal transplant rec...
journal_title:Transplantation
pub_type: 临床试验,杂志文章
doi:10.1097/00007890-199610150-00024
更新日期:1996-10-15 00:00:00
abstract::In a canine model of dog leukocyte antigen (DLA)-identical nonmyeloablative marrow transplantation including postgrafting immunosuppression with cyclosporine (CSP) and mycophenolate mofetil (MMF), engraftment was only transient with 100 cGy total body irradiation (TBI) conditioning, indicating suboptimal pretransplant...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000181168.42461.0b
更新日期:2005-11-27 00:00:00
abstract::Cell-mediated cytotoxicity directed toward pancreatic islets plays a significant role in the pathogenesis of type 1 diabetes mellitus. Histologically, autoimmune insulitis appears as mononuclear infiltrates that precede actual beta cell loss. Experimental studies by Bartlett et al. on the BB rat model suggest that who...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199612270-00014
更新日期:1996-12-27 00:00:00
abstract::One of the most powerful influences on cadaveric renal graft survival is the enhancing effect of blood transfusions from unrelated individuals (1, 2). However, the optimum number of transfusions required to achieve this effect remains controversial. Enhanced graft survival following only one or two transfusions has be...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198503000-00008
更新日期:1985-03-01 00:00:00
abstract:BACKGROUND:The impact of major histocompatibility class I chain-related A (MICA) antibodies on renal graft outcomes is unclear. The goal of this work was to assess the impact of posttransplant MICA antibodies, assayed at 1 year, with two commercially available kits, on long-term renal graft outcomes. METHODS:We retros...
journal_title:Transplantation
pub_type: 杂志文章,多中心研究
doi:10.1097/TP.0b013e31824fd8f1
更新日期:2012-06-27 00:00:00
abstract:BACKGROUND:In a randomized, controlled study we investigated the clinical efficacy of the microemulsion formulation of cyclosporine (Neoral) in comparison with Sandimmune (SIM) in the treatment of patients who underwent primary orthotopic liver transplantation (OLT). METHODS:In total, 33 patients were randomized in a ...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00007890-199709150-00011
更新日期:1997-09-15 00:00:00
abstract::Patients receiving allogeneic marrow transplantation for hematologic malignancies commonly are conditioned with total body irradiation (TBI) and given methotrexate (MTX) in an attempt to prevent graft-versus-host disease. To study the effects of TBI with or without MTX on bronchoalveolar cells and proteins, we perform...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198404000-00003
更新日期:1984-04-01 00:00:00
abstract:BACKGROUND:Imported pancreata accumulate cold ischemia time (CIT), limiting utilization and worsening outcomes. Flow cytometric crossmatching (FXM) is a standard method to assess recipient and donor compatibility, but can prolong CIT. Single-antigen bead assays allow for detection of recipient donor-specific HLA antibo...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001125
更新日期:2016-05-01 00:00:00
abstract::Since chemotaxis is an important mechanism by which leukocytes are recruited to allografts during rejection, the effect of immunosuppressant agents on in vitro chemotactic responses of neutrophils, monocytes, and lymphocytes was studied. Cyclosporine caused profound inhibition of lymphocyte chemotaxis to three differe...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199011000-00020
更新日期:1990-11-01 00:00:00
abstract::We report on the to date largest recovery of 11 organs from a single deceased donor with the transplantation of face, bilateral upper extremities, heart, 1 lung, liver (split for 2 recipients), kidneys, pancreas, and intestine. Although logistically challenging, this case demonstrates the feasibility and safety of the...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001200
更新日期:2016-10-01 00:00:00
abstract:BACKGROUND:The organ shortage has led many transplant centers to accept kidneys from old, suboptimal deceased donors, and make increasing use of old-for-old allocation systems. We report the experience of an Italian transplant center in the utilization of "ultra-old" (>75 years old) donors. METHODS:Sixty grafts from d...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181a6b4ff
更新日期:2009-06-27 00:00:00
abstract:BACKGROUND:Pediatric liver transplant recipients have traditionally been grouped according to age. Age-based classification schemes are useful in identifying clinical problems in selected age groups and also for developing solutions to these problems. Although infants in the first 3 months of life have not traditionall...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199809150-00010
更新日期:1998-09-15 00:00:00
abstract:BACKGROUND:The role of anti-human leukocyte antigen (HLA) donor-specific antibodies (DSA) detected by Luminex in the development of rejection is not fully understood. METHODS:A study including 369 patients who received transplants from deceased donors with a negative complement-dependent cytotoxicity crossmatch (XM) w...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31825ace2c
更新日期:2012-08-27 00:00:00
abstract:BACKGROUND:This 36-month, randomized, parallel-group study compared safety and efficacy of two doses of everolimus with mycophenolate mofetil (MMF) in de novo renal-transplant recipients. METHODS:Renal-allograft recipients received 1.5 mg/day or 3 mg/day of everolimus or 2 g/day of MMF, plus full-dose cyclosporine (Cs...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1097/01.tp.0000164352.65613.24
更新日期:2005-07-27 00:00:00
abstract:BACKGROUND:This study addresses the question of the mechanism for maintaining tolerance to donor alloantigen in the absence of antigen and the role of secondary lymphoid tissues. METHODS:Depleting anti-CD4 antibody administration in conjunction with allogeneic heart transplantation generates donor-specific operational...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000259928.16003.aa
更新日期:2007-05-15 00:00:00
abstract:BACKGROUND:Goal tacrolimus concentrations for the prevention of rejection in sensitized renal transplant recipients are not well established. METHODS:We evaluated the association between discharge tacrolimus trough concentration and the incidence of biopsy-proven acute rejection (BPAR) in 216 moderately sensitized ren...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000000149
更新日期:2014-05-27 00:00:00
abstract:BACKGROUND:Xenogeneic corneal fragments (guinea pig) are highly resistant to immune rejection in the anterior chamber of mouse eyes. Because guinea pigs and mice are discordant (i.e., mouse serum normally contains guinea pig reactive xenoantibodies), we wished to determine the extent to which xenogeneic corneal fragmen...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200002270-00025
更新日期:2000-02-27 00:00:00
abstract:BACKGROUND:Administration of donor bone marrow (BM)-derived dendritic cell (DC) progenitors (DCp) that are major histocompatibility complex (MHC) class II+ but costimulatory molecule (CD40, CD80, CD86)-deficient can prolong mouse heart allograft survival This is associated with microchimerism and inhibition of antidono...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199812270-00040
更新日期:1998-12-27 00:00:00
abstract:BACKGROUND:In Th (T helper) 1/Th2 balance in response to signals given during donor antigen presentation, induction of allograft prolongation is more often related to Th2-type than with Th1-type immunity. Here, we examined the effect of interleukin (IL)-33, a novel member of the IL-1 family, on cardiac allograft surviv...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181d720af
更新日期:2010-05-27 00:00:00
abstract::The complement system plays a major role in hyperacute rejection of discordant xenografts. In an immediately vascularized xenograft of a porcine organ to a primate, natural antibodies bind to the vascular endothelium of the graft, triggering activation of complement via the classical pathway. One consequence of antibo...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199311000-00024
更新日期:1993-11-01 00:00:00