Abstract:
:The complement system plays a major role in hyperacute rejection of discordant xenografts. In an immediately vascularized xenograft of a porcine organ to a primate, natural antibodies bind to the vascular endothelium of the graft, triggering activation of complement via the classical pathway. One consequence of antibody binding and complement activation is the activation of endothelial cells leading to the loss from the cells of heparan sulfate. We explored to what extent the classical pathway regulatory protein C1 inhibitor (C1 inh) would inhibit complement-mediated cytotoxicity and activation of endothelial cells. Cultured porcine aortic endothelial cells were used as a model for a xenogeneic organ and human serum as a source of xenoreactive natural antibody and complement. Addition of purified human C1 inh to human serum inhibited deposition of C4b and iC3b and cytotoxicity after the serum was reacted with the cultured cells. C1 inh prevented, in a dose-dependent manner, activation of the endothelial cells, as manifested by release of heparan sulfate. These observations demonstrate that C1 inh added in sufficient amounts to human serum can effectively inhibit C1 activation in an antigen-antibody system. These studies extend our previous findings consistent with the concept that complement activation occurs via the classical pathway in models of hyperacute rejection in which porcine vascular endothelial cells are in contact with human serum containing xenogeneic natural antibodies against the endothelial cells. Thus, our results suggest a potential clinical use of C1 inh in conjunction with other therapies to prevent hyperacute rejection in xenogeneic combinations mediated by complement activation via the classical pathway.
journal_name
Transplantationjournal_title
Transplantationauthors
Dalmasso AP,Platt JLdoi
10.1097/00007890-199311000-00024subject
Has Abstractpub_date
1993-11-01 00:00:00pages
1171-6issue
5eissn
0041-1337issn
1534-6080journal_volume
56pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:Transplantation enhances the risk of malignancies, due to the chronic use of antirejection medication. In the case of Kaposi's sarcoma (KS) the permissive effect of immunosuppression has been extensively studied, and cyclosporine (CsA) appears to play a key role. Here we have compared the incidence of KS in ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000173803.97398.31
更新日期:2005-09-27 00:00:00
abstract::The number of patients awaiting hepatic transplantation continues to exceed organ donation. As a result, many liver transplant candidates will develop life-threatening complications of their liver disease and not survive the pretransplant waiting period. Recent studies have demonstrated that hepatic lidocaine metaboli...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199304000-00028
更新日期:1993-04-01 00:00:00
abstract::Murine lymphoid cells sensitized against allografts express two types of cytotoxicity measured in vitro in short-term assays of 2 to 3 hr by 51Cr release. One type of cytotoxicity is specific against target cells carrying the sensitizing alloantigens, and the other type is nonspecific against both syngeneic and unrela...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197602000-00002
更新日期:1976-02-01 00:00:00
abstract::This study evaluated the utility of duplex Doppler sonograms (DS) and the resistive index (RI) in the identification and differential diagnosis of various causes of renal allograft dysfunction. The efficacy of DS and RI was studied either during acute episodes of allograft dysfunction or during periodic posttransplant...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199012000-00015
更新日期:1990-12-01 00:00:00
abstract::KM2210, a conjugate of estradiol and chlorambucil (CBL), which was originally developed as an anti-breast cancer agent, inhibits proliferative response of human mononuclear cells to alloantigens in mixed lymphocyte culture in a dose-dependent manner, but has no effect on their response to phytohemagglutinin. Neither e...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199210000-00022
更新日期:1992-10-01 00:00:00
abstract:BACKGROUND:Neonatal primates have been reported to receive pig hearts without hyperacute rejection (HAR). We examined the ontogeny of the anti-pig xenoantibody (XenoAb) and HAR in the neonatal and infant monkeys. METHODS:Twenty-six serum samples from 15 monkeys ages 14-192 days were subjected to hemagglutination titra...
journal_title:Transplantation
pub_type: 评论,杂志文章
doi:10.1097/00007890-200008270-00025
更新日期:2000-08-27 00:00:00
abstract:BACKGROUND:Hypoalbuminemia is associated with poorer outcomes in renal transplantation. Diabetes can compound hypoalbuminemia's detrimental effects. Kidney-pancreas transplantation alters the diabetic milieu; yet, some patients continue to be hypoalbuminemic. METHODS:We retrospectively analyzed 232 patients who underw...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199907150-00014
更新日期:1999-07-15 00:00:00
abstract::Allogeneic lymphocyte cytotoxicity (ALC) describes the elimination of allogeneic lymphocytes in vivo by an NK-related activity. There is evidence that ALC is demonstrable between donor and recipient when these are incompatible at MHC gene loci alone. Since ALC is a property of T cell-deficient nude rats, the role of t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198811000-00025
更新日期:1988-11-01 00:00:00
abstract::Potential female donors frequently ask whether unilateral nephrectomy will impair future childbearing capabilities. To address this question, we surveyed 220 women who underwent donor nephrectomy between 1985 and 1992. Of the 144 women who responded, 33 became pregnant after donation for a total of 45 pregnancies. Sev...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199612270-00044
更新日期:1996-12-27 00:00:00
abstract:BACKGROUND:Diarrhea is common in solid organ transplant recipients. Colonoscopy with random biopsies is performed frequently in the diagnostic evaluation of the posttransplant population with diarrhea. The purpose of this study was to determine the sensitivity of colonoscopy with random biopsy in determining a specific...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181ae98ab
更新日期:2009-08-15 00:00:00
abstract:BACKGROUND:Hemostatic complications are not uncommon after bone marrow transplantation (BMT). However, little is known about the frequency, localization, determinants, and outcome of hemostatic events in autologous and allogeneic BMT. METHODS:Four hundred forty-seven patients (364 allogeneic, 83 autologous transplants...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200211150-00018
更新日期:2002-11-15 00:00:00
abstract:BACKGROUND:New insights into mechanisms of injury in orthotopic liver transplantation (OLT) often come from experiments in small animal models. Mice are particularly suitable because of the growing availability of gene-altered animals and specific antibodies. A validated model of OLT in mice is not available and, in pa...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200211150-00008
更新日期:2002-11-15 00:00:00
abstract::T cell receptor (TCR) beta-chain usage by HLA-DR1 alloreactive T cell lines was examined to determine whether common TCR gene segments were used preferentially. We have demonstrated previously that a DR1-specific, human renal allograft-derived T cell line and replicate, anti-DR1 mixed lymphocyte reactions (MLR), estab...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199604150-00017
更新日期:1996-04-15 00:00:00
abstract:BACKGROUND:Acute kidney injury (AKI) after living donor liver transplantation (LDLT) is associated with increased mortality. We sought to identify associations between intraoperative hemodynamic variables and postoperative AKI. METHODS:We retrospectively reviewed 734 cases of LDLT. Intraoperative hemodynamic variables...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002584
更新日期:2019-09-01 00:00:00
abstract::Orthotopic liver transplantation is frequently associated with a complex coagulation disorder, influencing the outcome of the procedure. In this respect, disseminated intravascular coagulation (DIC) had been suggested to be of causative importance for bleeding complications after reperfusion of the liver graft. In 10 ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199109000-00018
更新日期:1991-09-01 00:00:00
abstract:BACKGROUND:Membrane CD30 is an important costimulatory molecule for activated T lymphocytes, and serum level of soluble CD30 (sCD30) is considered a marker for predicting outcome in kidney transplantation. METHODS:We investigated the kinetics of CD30 expression on CD4 and CD8 T-cell populations and the source of sCD30...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e318296fd69
更新日期:2013-07-27 00:00:00
abstract::Spleen cells obtained from C57BL/Ks (Ks, H-2d) mice carrying passively enhanced Sarcoma I (Sa I, H-2a) tumors were tested for alloantibody formation, lymphocyte blastogenesis, antibody-dependent cellular cytotoxicity, and cell to cell cytotoxicity. Assays were usually performed approximately 6 weeks after tumor inocul...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197803000-00011
更新日期:1978-03-01 00:00:00
abstract::The aim of this study was to assess directly the function of isolated hepatocytes 1 year after transplantation into the spleen, using an original model of isolated rat-spleen perfusion. Three specific liver functions, albumin synthesis, indocyanine-green clearance, and antipyrine oxidation, were studied. Five x 10(6) ...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract:BACKGROUND:The spleen is considered to be an important secondary lymphoid organ where acute graft-versus-host disease (GVHD) is initiated by donor T cells that recognize host alloantigens after allogeneic bone-marrow transplantation (BMT). The influence of splenectomy on the development of GVHD prior to BMT has yet to ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200205270-00027
更新日期:2002-05-27 00:00:00
abstract:BACKGROUND:We assess whether remote ischemic preconditioning (rIPC) of the recipient can modify ischemia-reperfusion (IR) injury in the donor heart following orthotopic heart transplantation from brain dead donors and to examine potential mechanisms of protection. METHODS:Sixteen pigs weighing from 26 to 34.2 (mean 29...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000159137.76400.5d
更新日期:2005-06-27 00:00:00
abstract:BACKGROUND:Ischemia-reperfusion syndrome has been recognized as an important pathogenic factor in renal transplantation, not only in the development of delayed graft function but also in the development of acute and chronic rejection. Hypoxia-inducible factor (HIF)-1 activates transcription of several genes implicated ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000063128.86981.B2
更新日期:2003-05-27 00:00:00
abstract:BACKGROUND:The National Marrow Donor Program (NMDP) has approximately 5 million registered potential donors and continues to add more than 30,000 new recruits to the registry monthly. However, more than 30% of potential donors are not available or decline to donate at key decision points in the donation process. Althou...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000116394.88804.a7
更新日期:2004-03-27 00:00:00
abstract::We studied the reactivity of cardiac graft-infiltrating cells, cultured from endomyocardial biopsy specimens, toward heart endothelial cells (Hec). In two cases, Hec derived from the specific donor heart or Hec compatible with the donor were lysed, but not the syngeneic B cell line. A vessel-derived endothelial cell l...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199608150-00017
更新日期:1996-08-15 00:00:00
abstract::Hepatocyte transplantation (HT) has been performed in patients with liver-based metabolic disease and acute liver failure as a potential alternative to liver transplantation. The results are encouraging in genetic liver conditions where HT can replace the missing enzyme or protein. However, there are limitations to th...
journal_title:Transplantation
pub_type: 社论,评审
doi:10.1097/TP.0b013e31823b72d6
更新日期:2012-02-27 00:00:00
abstract::Despite growing evidence that reactive oxygen species are responsible for deleterious effects of ischemia-reperfusion (I/R) injury of the liver, there exists, until now, no reliable antioxidant therapeutics applicable in the clinical setting. We investigated the effects of free radical scavenger, 3-methyl-1-phenyl-2-p...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200211270-00021
更新日期:2002-11-27 00:00:00
abstract::In a DBA/2 (H-2d) pancreatic islet-to-B6AF1 (H-2b/k.d) recipient combination, the graft survival of hand-picked islets was compared with that of "crude digested" islets that were prepared simply by collagenase digestion and Ficoll gradient separation and were contaminated with lymph nodes and vascular and ductal tissu...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198610000-00011
更新日期:1986-10-01 00:00:00
abstract:BACKGROUND:Since the Edmonton trial in 2000, increasing numbers of transplant centers have been implementing islet transplantation programs. Some institutions have elected to associate in multicenter networks, such as the Swiss-French GRAGIL (Groupe Rhin-Rhône-Alpes-Genève pour la Transplantation d'Ilots de Langerhans)...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000161224.67535.41
更新日期:2005-05-15 00:00:00
abstract::Tacrolimus (FK) and cyclosporine (Cs) are potent immunosuppressants that effectively prevent the rejection of transplanted organs including liver and small intestine. Our study examined the effects of these immunosuppressants on Peyer's patches, which play an important role in mucosal immune response through uptake an...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200204150-00006
更新日期:2002-04-15 00:00:00
abstract::Antibody-mediated rejection (AMR) is an important cause of graft loss and continues to present a formidable obstacle to successful transplantation. Unresolved problems continue to be the absence of effective strategies to ablate the donor-specific antibody (DSA) response as well as to attenuate the antibody-mediated g...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000003298
更新日期:2021-02-01 00:00:00
abstract::OKT3, an anti-CD3 MAB, depletes T cells in vivo and is among the most potent inhibitors of acute allograft rejection. The mechanism of this inhibition is unknown. The present studies investigate whether anti-CD3 antibodies have the ability to crosslink CD3 on two different cells and induce TCR-dependent antibody-bridg...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199010000-00030
更新日期:1990-10-01 00:00:00