Abstract:
:To facilitate the study of the CaMKIIα function in vivo, a CaMKIIα-GFP transgenic mouse line was generated. Here, our goal is to provide the first neuroanatomical characterization of GFP expression in the CNS of this line of mouse. Overall, CaMKIIα-GFP expression is strong and highly heterogeneous, with the dentate gyrus of the hippocampus as the most abundantly expressed region. In the hippocampus, around 70% of granule and pyramidal neurons expressed strong GFP. In the neocortex, presumed pyramidal neurons were GFP positive: around 32% of layer II/III and 35% of layer VI neurons expressed GFP, and a lower expression rate was found in other layers. In the thalamus and hypothalamus, strong GFP signals were detected in the neuropil. GFP-positive cells were also found in many other regions such as the spinal trigeminal nucleus, cerebellum and basal ganglia. We further compared the GFP expression with specific antibody staining for CaMKIIα and GABA. We found that GFP+ neurons were mostly positive for CaMKIIα-IR throughout the brain, with some exceptions throughout the brain, especially in the deeper layers of neocortex. GFP and GABA-IR marked distinct neuronal populations in most brain regions with the exception of granule cells in the olfactory bulb, purkinje cells in the cerebellar, and some layer I cells in neocortex. In conclusion, GFP expression in the CaMKIIα-GFP mice is similar to the endogenous expression of CaMKIIα protein, thus these mice can be used in in vivo and in vitro physiological studies in which visualization of CaMKIIα- neuronal populations is required.
journal_name
Brain Resjournal_title
Brain researchauthors
Wang X,Zhang C,Szábo G,Sun QQdoi
10.1016/j.brainres.2013.04.042subject
Has Abstractpub_date
2013-06-26 00:00:00pages
9-25eissn
0006-8993issn
1872-6240pii
S0006-8993(13)00584-2journal_volume
1518pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Eleven beta-carbolinium compounds (beta C+s) and MPP+ were stereotaxically injected (40-200 nmol in 5 microliter of vehicle) unilaterally into the substantia nigra of anesthetized adult male Sprague-Dawley rats. The rats were sacrificed after three weeks. The ipsilateral striatum was analyzed for dopamine and DOPAC le...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00082-2
更新日期:1995-03-27 00:00:00
abstract::Using a perifusion culture technique, the pattern of vasopressin release from the suprachiasmatic nucleus was studied in vitro under basal and stimulated conditions. Individual rat suprachiasmatic explants released vasopressin in a rhythmic fashion and these rhythms persisted with a circadian period for up to 4 cycles...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90952-8
更新日期:1987-10-06 00:00:00
abstract::Previous studies have shown behavioral changes in pre- and postnatal rats exposed to morphine before and during gestation. The present study has attempted to discover morphological correlates of these changes in the fetal rat spinal cord. Pregnant Wistar rats were injected subcutaneously on days 12-18 of gestation wit...
journal_title:Brain research
pub_type: 杂志文章
doi:
更新日期:1980-11-24 00:00:00
abstract::Double impregnation staining of tissue was used to study on thick sections the three-dimensional configuration of the peripheral endoplasmic reticulum in neurosecretory neurons of the supraoptic nucleus in control and water-deprived rats. According to the spatial organization of the endoplasmic reticulum, two types of...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(79)90105-7
更新日期:1979-07-13 00:00:00
abstract::Glucocorticoid receptor (Gr) concentrations in the rat brain are low at the time of birth and increase towards adult levels during the first two weeks of life. Brief, daily handling of pups for the first 21 days postnatally has been found to permanently increase Gr concentrations within the hippocampus. Thus, the deve...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(85)90183-x
更新日期:1985-10-01 00:00:00
abstract::Effects of iontophoretically applied dopamine were investigated in prefrontal neurons of the monkey which showed activity changes during a visual reaction time task. The task consisted of an initial waiting phase (3.0 s), a warning phase (green lamp, 1.5-3.5 s), a lever-release GO phase (red lamp) and a reward phase. ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)90385-9
更新日期:1986-04-23 00:00:00
abstract::The response of the endoneurial vasculature in rat sciatic nerve following crush injury was investigated by morphometric analysis of serial nerve transverse sections at the site of injury and in distal segments at 1, 2, 3, 6, and 9 weeks after injury. Quantitative analysis included determination of the number of vesse...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90018-i
更新日期:1993-09-24 00:00:00
abstract::Previous studies have shown that access to conceptual/semantic information precedes phonological access in alphabetic language production such as English or Dutch. The present study investigated the time course of semantic and orthographic encoding in Chinese (a non-alphabetic language) spoken word production. Partici...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.03.049
更新日期:2009-06-01 00:00:00
abstract::The effects of intracerebroventricular (i.c.v.) infusion of arginine vasopressin (AVP) on intracranial pressure (ICP), blood pressure (BP) and plasma AVP were investigated in conscious goats. The animals were implanted with ventricular (V) and cisternal (C) cannulae under halothane anaesthesia and allowed to recover p...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90850-x
更新日期:1987-10-13 00:00:00
abstract::Alpha-synuclein is a natively unfolded protein playing a key role in the regulation of several neuronal synaptic functions in physiological and pathological conditions. Many studies, over the past years, have shown that it is actively involved in PD pathophysiology. Alpha-synuclein is integrated in a complex network o...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2012.04.014
更新日期:2012-10-02 00:00:00
abstract::Electrical stimulation of the sural nerve (SN) revealed input from sural nerve afferents to L6 and L7 dorsal horn neurons that were not apparent using natural mechanical stimuli, especially in cells with variable latency responses to SN stimulation. Nearly all (31/32) cells that had reliable, fixed latency responses t...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)90816-4
更新日期:1986-04-16 00:00:00
abstract::Spontaneous hippocampal EEG activity and evoked field potentials were investigated in intact rats and in animals with fetal hippocampal grafts. Pieces of hippocampal grafts, derived from 15- to 16-day-old fetuses, were used to prepare cell suspensions and grafted directly into the intact hippocampus. Control animals r...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90168-u
更新日期:1991-07-19 00:00:00
abstract::Unilateral 6-hydroxydopamine (6-OHDA) lesions of substantia nigra pars compacta (SNPC) neurons in rats induce behavioural hypersensitivity to dopaminergic agonists. However, the role of specific dopamine receptors is unclear, and potential alterations in their transduction mechanisms remain to be evaluated. The presen...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)02927-4
更新日期:2001-11-30 00:00:00
abstract::Intravenous administration of the cholecystokinin (CCK) antagonist lorglumide (LORG) reversed chronic haloperidol (CHAL)-induced depolarization inactivation (DI) of dopamine (DA) cells in both the A9 and A10 areas. Moreover, microinjection of LORG, but not naloxone, directly into the medial nucleus accumbens (mNAc) do...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)90330-7
更新日期:1988-11-08 00:00:00
abstract::Rat striata were exposed to 15 mM quinolinic acid (QUIN), or QUIN plus the nitric oxide synthase inhibitors S-methyl-L-thiocitrulline dihydrochloride (L-MIN) or 7-nitroindazole monosodium salt (7-NINA) for 21 days. Co-administration of 100 microM or 1 mM L-MIN with QUIN significantly reduced lesion volume compared to ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00962-1
更新日期:1997-11-14 00:00:00
abstract::The purpose of this work was to characterize the gap junctions between cultured glomus cells of the rat carotid body and to assess the effects of acidity and accompanying changes in [Ca(2+)](i) on electric coupling. Dual voltage clamping of coupled glomus cells showed a mean macrojunctional conductance (G(j)) of 1.16 ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)02862-1
更新日期:2001-10-19 00:00:00
abstract::We have previously reported progressive reactive astrocytes in the cerebellum of taiep rats, one of the most regions affected by demyelination, and activation of cerebellar glial cells in vitro. Based on the hypothesis that activated glial cells produce high levels of reactive nitrogen intermediates, we assessed the p...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.08.097
更新日期:2006-11-22 00:00:00
abstract::Thalamocortical connections were studied after injections of retrograde tracers were made into rostral or caudal parts of cortical area Fr2 in adult rats. The data reveal that both rostral Fr2 (rFr2) and caudal Fr2 (cFr2) receive input from the centrolateral, central medial, interanteromedial, mediodorsal, paracentral...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)91420-6
更新日期:1991-12-24 00:00:00
abstract::The effects of an intensive short-term glucocorticoid (e.g. triamcinolone) regimen in cats have been studied on the actions of the dopamine (DA) receptor agonist apomorphine (APO) on spinal lumbar primary afferent excitability (dorsal root reflex or DRR) and monosynaptic reflex (MSR) transmission. Glucocorticoid dosin...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90894-6
更新日期:1983-05-16 00:00:00
abstract::Tuberohypophysial dopamine (DA) neurons terminate in the intermediate and neural lobes of the posterior pituitary. The objective of this study was to determine if concentrations of 3,4-dihydroxyphenylacetic acid (DOPAC), a major metabolite of DA in these regions, reflect the activity of tuberohypophysial DA neurons. T...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)91209-y
更新日期:1990-01-01 00:00:00
abstract::Calretinin and calbindin-D28k are two calcium-binding proteins which are present in separate populations of interneurons in cerebral cortex and hippocampus. To identify these cells with the populations expressing different transmitters, two-colour immunofluorescence was done with antibodies against the calcium-binding...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)91439-l
更新日期:1992-07-31 00:00:00
abstract::Acute administration of diazepam (2 mg/kg i.p.) to rats decreased the number of head-dips, locomotor activity and the number of rears made in the holeboard apparatus, indicating sedative effects. After daily treatment for 7 days with diazepam (2 mg/kg) tolerance developed to all these behavioural effects, despite seru...
journal_title:Brain research
pub_type: 杂志文章
doi:
更新日期:1996-09-23 00:00:00
abstract::Novel environments induce a conflicting emotional approach-withdrawal state that triggers stress-related reactions. Social support through the presence of a highly familiar conspecific buffers the individual against such challenges. Although aversive events seem to be predominantly processed by the right hemisphere, t...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2020.147198
更新日期:2021-01-15 00:00:00
abstract::The content and turnover of dopamine, norepinephrine and 5-hydroxytryptamine (serotonin), and the content of their respective major metabolites were evaluated in 19 discrete brain areas of rats exposed to conditioned emotional response (CER), and in control groups which received either equivalent yoked shock (shock on...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90647-3
更新日期:1982-05-20 00:00:00
abstract::The effects of natural (mechanical and gustatory) stimulation of the nasopharynx or electrical stimulation of the pharyngeal branch of the glossopharyngeal (PH-IXth) nerve on the changes in heart rate (HR) and arterial blood pressure (BP) were investigated in paralyzed and anesthetized rats. Afferent responses in the ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91614-x
更新日期:1993-08-13 00:00:00
abstract::The involvement of the nucleus intercollicularis (ICo) region of the midbrain, an estrogen-sensitive area, in the expression of estrogen-dependent female courtship behavior was examined with radiofrequency lesion and intracranial hormone implant techniques. Bilateral lesions in the ICo region caused a reduction in the...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)90365-6
更新日期:1981-03-02 00:00:00
abstract::Reactive oxygen species (ROS) are supposed to be involved in neurodegenerative processes like Parkinson's or Alzheimer's disease. Beside this there are an increasing number of studies indicating an involvement of ROS in traumatic brain injury. We therefore studied the potential role of astrocytes against neurotoxic ef...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(96)01029-3
更新日期:1996-12-16 00:00:00
abstract::The effects of two drugs with anxiolytic actions, diazepam (0.1, 0.3 and 1 mg/kg) and nicotine (0.1 and 0.5 mg/kg) were examined on the time spent in social interaction by pairs of male gerbils. In a test arena lit by high light, diazepam (0.1 mg/kg) increased social interaction, without changing locomotor activity. D...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)03102-4
更新日期:2001-01-12 00:00:00
abstract::Centrifugal fibers and their synaptic connections were studied in retinas of the lamprey Lampetra fluviatilis. The morphological analysis of retinofugal and retinopetal elements was performed after their horseradish peroxidase (HRP) filling through either the cut optic nerve in isolated retina preparations or after in...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90999-2
更新日期:1989-07-24 00:00:00
abstract::The effects of recombinant interleukin-2 (rIL-2) on the potentiation of the synaptic transmission were studied in rat hippocampal slices by using extracellular field potential recordings. The application of rIL-2 inhibited the induction of both short-term (STP) and long-term potentiation (LTP) in a dose-dependent mann...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)91331-a
更新日期:1990-08-13 00:00:00