Abstract:
:In Western countries, chronic hepatitis C virus (HCV)-infection mostly affects former and active substance users. The effect of active substance use on interferon (IFN)-responsiveness and therapy efficacy is not well understood. In this study, we compared natural killer (NK) cell activity and function in healthy controls and chronic HCV-infected patients with and without active substance use, as well as the early effects of antiviral therapy with peg-IFN and ribavirin. No differences were observed between chronic HCV patients and healthy individuals in the number and frequencies of CD56(dim) and CD56(bright) NK cells. Also, IL-12/18-induced IFN-gamma production by NK cells was comparable between all groups, whereas the cytotoxic ability of NK cells (granzyme and CD107a levels) was more potent in HCV-infected patients as compared to healthy controls, and highest in non-substance users. Moreover, at baseline, the activation of NK cells was significantly lower in HCV-infected patients who used substances, when compared to healthy individuals. Therapy-induced viral load reduction assessed early at day 7 showed a similar decline in substance users and non-substance use HCV patients, with 25% substance users and 17% non-substance users testing HCV-RNA negative at day 7. Furthermore, early during IFN-based therapy, NK cells from HCV patients remained responsive to IFN, and only a minor decline in the degree of STAT-1 phosphorylation was observed irrespective of substance use. These findings were further supported by comparable in vitro p-STAT-1 induction in all three experimental groups. Despite subtle differences at baseline between healthy individuals and chronic HCV patients, we observed that active substance use in chronic HCV-infected patients did not affect the immune responsiveness to IFN early after start of treatment, and thus, we found no evidence - from an immunological point of view - that antiviral therapy of our cohort of HCV-infected patients with active substance use is less efficient.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Hotho DM,Kreefft K,Groothuismink ZM,Janssen HL,de Knegt RJ,Boonstra Adoi
10.1016/j.antiviral.2012.12.025subject
Has Abstractpub_date
2013-03-01 00:00:00pages
347-55issue
3eissn
0166-3542issn
1872-9096pii
S0166-3542(12)00316-6journal_volume
97pub_type
杂志文章abstract::Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hoofed animals. We have reported (Cubillos et al., 2008) that a synthetic dendrimeric peptide consisting of four copies of a B-cell epitope [VP1(136-154)] linked through thioether bonds to a T-cell epitope [3A(21-35)] of FMDV [B4T(thi)] e...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.03.005
更新日期:2016-05-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2005.05.001
更新日期:2005-08-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2009.09.010
更新日期:2009-12-01 00:00:00
abstract::The Cytomegalovirus Retinitis Retreatment Trial was a multicenter clinical trial designed to evaluate three treatments for the treatment of relapsed CMV retinitis; (1) foscarnet; (2) 'high-dose' ganciclovir, and (3) combination foscarnet and ganciclovir. Two hundred seventy-nine patients were enrolled and randomly ass...
journal_title:Antiviral research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0166-3542(95)00921-3
更新日期:1996-01-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2008.07.002
更新日期:2008-12-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(99)00067-4
更新日期:1999-12-31 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/s0166-3542(01)00175-9
更新日期:2001-11-01 00:00:00
abstract::Interferon alpha-2b (IFN) alone or in combination with Ribavirin is approved in the United States for the treatment of chronic hepatitis C virus (HCV) infection. We have previously reported that the glucosidase inhibitor, n-butyl deoxynojirimycin (nB-DNJ) inhibits the production of infectious bovine diarrhea virus (BV...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(02)00075-x
更新日期:2002-09-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2017.03.024
更新日期:2017-06-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2010.04.015
更新日期:2010-08-01 00:00:00
abstract::Chikungunya virus (CHIKV) is an arthropod-borne alphavirus. Alphaviruses are positive strand RNA viruses that require a 5' cap structure to direct translation of the viral polyprotein and prevent degradation of the viral RNA genome by host cell nucleases. Formation of the 5' RNA cap is orchestrated by the viral protei...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.03.013
更新日期:2018-06-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2017.05.009
更新日期:2017-08-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(01)00205-4
更新日期:2002-02-01 00:00:00
abstract::Preclinical evaluation of the effectiveness of interferon (IFN) therapy on human papillomaviruses (HPV) has been hampered by the inability to propagate these viruses in cell culture. Nonetheless, interferon is used extensively in the treatment of HPV infections. Alpha interferons in particular have found a place in th...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/0166-3542(94)90066-3
更新日期:1994-07-01 00:00:00
abstract::Lentiviral vectors have been shown to be good candidates for gene transfer protocols; however, prevention of insertional mutagenesis remains problematic. Here we report on the design of a conditionally replicating integrase (IN)-defective lentiviral-hybrid episomal vector in which the insertion of the SV40 promoter/or...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2008.06.015
更新日期:2008-12-01 00:00:00
abstract::Three water-soluble derivatives of amphotericin B were tested for inhibition of HIV infection in vitro. The compounds amphotericin B methyl ester (AME) and N-(N'-(2-(4'-methylmorpholinio)ethyl)N"-cyclohexyl guanyl) amphotericin B methyl ester (MCG) inhibited HIV infection by 50% at 1 microgram/ml; N-(N'-(3-dimethylami...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(90)90031-2
更新日期:1990-09-01 00:00:00
abstract::High-throughput genomic methods are now being used to study a wide variety of viral diseases, in an effort to understand how host responses to infection can lead either to efficient elimination of the pathogen or the development of severe disease. This article reviews how gene expression studies are addressing importa...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2008.12.005
更新日期:2009-03-01 00:00:00
abstract::Respiratory syncytial virus (RSV) infection causes significant disease in the lower respiratory tract of young children, and there is currently no licensed vaccine to prevent RSV infection. The F glycoprotein is considered a major antigenic target for RSV vaccine development. Recent evidence indicates that the pre-fus...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2019.02.017
更新日期:2019-05-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2012.02.012
更新日期:2012-05-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2015.08.016
更新日期:2015-11-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2015.07.003
更新日期:2015-09-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2019.104650
更新日期:2020-01-01 00:00:00
abstract::Site-directed in vitro mutagenesis was used to create analogs of human interferons (IFNs)-alpha 1 and -alpha 4. Analogs were expressed in vitro using SP6 RNA polymerase and a rabbit reticulocyte lysate cell-free protein synthesis system. Amino acid substitutions for the highly conserved residues at positions 33, 121, ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(89)90066-1
更新日期:1989-08-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(02)00051-7
更新日期:2002-08-01 00:00:00
abstract::Rotavirus infection has emerged as an important cause of complications in organ transplantation recipients and might play a role in the pathogenesis of inflammatory bowel disease (IBD). 6-Thioguanine (6-TG) has been widely used as an immunosuppressive drug for organ recipients and treatment of IBD in the clinic. This ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.06.011
更新日期:2018-08-01 00:00:00
abstract::Cell-to-cell contact is usually essential for syncytium formation by HTLV-I-infected cell lines. The present study was undertaken to determine the inhibitory effect of polyanionic compounds, dextran sulfate and heparin, on HTLV-I-induced syncytium formation, as demonstrated by the fusion of HTLV-I-infected cells with ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(94)90041-8
更新日期:1994-02-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2021.105014
更新日期:2021-01-07 00:00:00
abstract::The role of herpes simplex virus type 1 (HSV-1)-encoded ribonucleotide reductase (RR) has been investigated in mice and guinea pigs using a mutant from which 90% of the large subunit of the enzyme was deleted. The RR mutant was extremely impaired in its ability to induce external vaginal lesions or to cause death in m...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(92)90048-a
更新日期:1992-02-01 00:00:00
abstract::Although canine rabies has been eliminated from industrialized countries, infected dogs remain the primary source of human and livestock exposures in Asia, Africa and much of South America. Human deaths are the most important direct economic impact of canine rabies, followed by livestock losses and the cost of PEP, wh...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2013.03.004
更新日期:2013-05-01 00:00:00
abstract::The first 230 residues of the 298-amino acid glycoprotein G of respiratory syncytial virus (RSV) are sufficient to confer complete resistance to challenge with live RSV, whereas the first 180 residues completely failed (Olmsted et al. (1989) J. Virol. 63, 411-420). The characterization of a protective epitope correspo...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(95)00053-4
更新日期:1995-12-01 00:00:00