Abstract：
:Fluoxetine shows controversial lung effects as it prevents pulmonary hypertension in adult rats but exposure during gestation causes pulmonary hypertension in neonatal rats. In the present study, we tested the null hypothesis that the antidepressant drug fluoxetine does not modify the development of bronchopulmonary dysplasia (BPD) in neonatal rats. Experimental categories included I: room air (controls) with daily injection of saline; II: room air with daily injection of 10 mg/kg fluoxetine, i.p., during two weeks; III: 60% oxygen with daily injection of saline; and IV: 60% oxygen with daily injection of 10 mg/kg fluoxetine, i.p., during two weeks. Hyperoxia resulted in significant reduction in alveolar density and an increase in pulmonary endocrine cells, as well as increases in muscle layer areas of bronchi and arteries. Fluoxetine treatment generated a further increase in muscularisation and did not significantly modify the hyperoxia-induced reductions in alveolar density and increases in the endocrine cells. In hyperoxia, Real-Time PCR showed a lower pulmonary expression of vascular endothelial growth factor (VEGF) with no significant changes in the expression of matrix metalloproteinases (MMP) 2 and 12. Fluoxetine did not affect VEGF or MMP-2 expression but it significantly increased MMP-12 mRNA in both normoxic and hyperoxic groups. Zymographic analysis of MMP-2 activity in bronchoalveolar fluid showed a significantly reduced MMP-2 activity in hyperoxia, while fluoxetine treatment restored MMP-2 activity to levels comparable with the normoxic group. In conclusion, our data show that fluoxetine may worsen bronchial and arterial muscularisation during development of BPD and may up-regulate MMP expression or activity.
journal_name
Histol Histopatholjournal_title
Histology and histopathologyauthors
Porzionato A,Zaramella P,Macchi V,Grisafi D,Salmaso R,Baraldi M,Fornaro E,Tassone E,Masola V,Onisto M,Chiandetti L,De Caro Rdoi
10.14670/HH-27.1599subject
Has Abstractpub_date
2012-12-01 00:00:00pages
1599-610issue
12eissn
0213-3911issn
1699-5848journal_volume
27pub_type
杂志文章abstract：:Osteoclasts are physiological polykaryons specialized in the resorption of calcified tissue. In the context of the clinical use of calcium-phosphate (CaP) ceramics as bone substitutes, this study used transmission electron microscopy to investigate the in vitro mechanisms of CaP ceramic degradation by osteoclastic cel...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-16.37
更新日期：2001-01-01 00:00:00
abstract：:The present study describes the use of a microwave processing protocol for the rapid histopathological and immunohistochemical diagnosis of bovine rabies. Immunohistochemistry has been used for rabies diagnosis in formalin-fixed tissue with satisfactory results, although the time to diagnosis is considerably longer th...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-27.1227
更新日期：2012-09-01 00:00:00
abstract：:Actin microfilaments and microtubules are important cytoskeletal proteins that regulate endothelial repair through alterations in cell shape and through regulation of cell migration following wounding of the endothelium. Upstream pathways have been identified in the regulation of actin and microtubule organization, es...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-18.879
更新日期：2003-07-01 00:00:00
abstract：OBJECTIVE:Describe the morphological changes that take place in the lung parenchyma and in the airways during the respiratory cycle with a view to establishing a relationship between them. SUBJECTS:Adult Wistar rats. INTERVENTIONS:The lungs were fixed at seven different points in the respiratory cycle: Inflation, 10 ...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-22.43
更新日期：2007-01-01 00:00:00
abstract：:The renin-angiotensin system (RAS) and tubuloglomerular feedback (TGF) are central to the maintenance of blood pressure and body fluid composition. Renin, NO synthase-1 (NOS-1), and cyclooxygenase-2 (COX-2) are key regulators of the RAS and TGF. In the present study, to investigate species-specific differences in the ...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-23.143
更新日期：2008-02-01 00:00:00
abstract：:Angiogenesis and bone formation are coupled during skeletal development and fracture healing. This relationship, although known for some time, has not been properly explored. Advances in the discovery of how angiogenesis is regulated in physiological processes like embryogenesis, endometrial regeneration and wound hea...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-27.559
更新日期：2012-05-01 00:00:00
abstract：:Pulmonary fibrosis is a common response to various injuries to the lung. The resolution of a fibroproliferative response after lung injury is key to survival. Although there are various initiating factors or causes, the terminal stages are characterized by proliferation and progressive accumulation of connective tissu...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-19.867
更新日期：2004-07-01 00:00:00
abstract：:Spermatogenesis is under the control of a complex endocrine and paracrine system, including estrogen receptor (ER) signaling. In many target cells, ER promotes the transcription of c-fos and other proto-oncogenes to regulate cell growth and differentiation. Thus, in this study we evaluated the expression of the proto-...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-24.1515
更新日期：2009-12-01 00:00:00
abstract：:The pineal organs of 14-week-old domestic geese were investigated with light and electron microscopy. The pineals consisted of a wide distal part and a narrow middle-proximal one. The glands were attached to the intercommissural region via the choroid plexus. The pineal parenchyma was formed by round or elongated foll...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-21.1075
更新日期：2006-10-01 00:00:00
abstract：:The resistance to airflow that develops in most obstructive processes takes place in the small airways. The aim of the present paper is to describe bronchial hysteresis morphometrically in a respiratory cycle model. As a working hypothesis, it is proposed that the changes that take place in the respiratory tract durin...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-18.19
更新日期：2003-01-01 00:00:00
abstract：BACKGROUND:Protein tyrosine kinase 7 (PTK7) is a member of receptor protein tyrosine kinase-like molecules, which is involved in tumorigenesis. However, the association between PTK7 expression and its pathological significance in survival prognosis remains under investigation. The purpose of this meta-analysis is to cl...
journal_title：Histology and histopathology
pub_type： 杂志文章,meta分析
doi：10.14670/HH-11-933
更新日期：2018-04-01 00:00:00
abstract：:Gp96 is the endoplasmic reticulum (ER)-resident molecular chaperone, which is involved in the correction of unfolded proteins, in the activation of proteasome-dependent ER-associated degradation of the misfolded proteins, and in activation of the protein translation that modulates polypeptide traffic into the ER. Furt...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-28.61
更新日期：2013-01-01 00:00:00
abstract：:Bacillary angiomatosis (BA) is a reactive vasoproliferative lesion occurring almost exclusively in immunocompromised individuals in response to infection by a bacillus closely related to Rochalimaea quintana. The commonest site of involvement is the skin, in the form of multiple erythematous nodules, but bacillary ang...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND:Expression of hypoxia-related tissue factors in 1p-aberrant oligodendroglial neoplasms diminishes patient outcome. Differentiated embryo-chondrocyte expressed gene 1 (DEC1) has been described as novel hypoxia-related tissue factor. In our study, we assessed the expression of DEC1 in 1p aberrant oligodendrogl...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-20.1173
更新日期：2005-10-01 00:00:00
abstract：:Compliance with current regulations for the development of innovative medicines require the testing of candidate therapies in relevant translational animal models prior to human use. This poses a great challenge when the drug is composed of cells, not only because of the living nature of the active ingredient but also...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-18-249
更新日期：2020-09-11 00:00:00
abstract：:Sex steroid hormones are involved in regulation of skin development and functions as well as in some skin pathological events. To determine the sites of action of estrogens, androgens and progestins, studies have been performed during the recent years to accurately localize receptors for each steroid hormone in human ...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-19.629
更新日期：2004-04-01 00:00:00
abstract：:Griffonia simplicifolia agglutinin I-B4 (GSAI-B4) has broader specificity for B antigen variants and can recognize the antigens in a wide variety of human tissues. Thus, the concentration range of GSAI-B4 required for staining and the susceptibility of staining to alpha-galactosidase digestion is presumed to correlate...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：:This study evidences the presence of primordial germ cells, in tissue squash preparations and sections from hematological malignancies. Primordial germ cells were identified by their morphology, the intense PAS, PAS-D reaction and presence of calcium-activated neutral proteinase. Primordial germ cells gave rise to nuc...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
abstract：:The terms construct or tissue equivalent refer to neotissue produced by tissue engineering techniques. The elements forming the construct are scaffolds on which cells are "recreated" to form an engineered-tissue sensitive to certain cell signals. The ability of the cells to expand and differentiate on the scaffold is ...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-20.891
更新日期：2005-07-01 00:00:00
abstract：:Chaperone-mediated autophagy (CMA), a selective form of autophagy, where cellular proteins with KFERQ-like motif are targeted to the lysosome for degradation, is necessary to maintain cellular homeostasis. The role of CMA in neurodegenerative diseases has been extensively studied in the past decades, with defects in t...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-18-202
更新日期：2020-07-01 00:00:00
abstract：:In the lungs, neuronal M2 muscarinic receptors limit the release of acetylcholine from postganglionic cholinergic nerves. However, these receptors are not functional under certain circumstances in animal models of hyperreactivity such as occurs after exposure of sensitised animals to an allergen or during a respirator...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-15.861
更新日期：2000-07-01 00:00:00
abstract：:Tumorigenesis correlates with hypermethylation of tumor suppressors and hypomethylation of oncogenes. DNA methyltransferases (DNMTs) catalyze DNA methylation, and mutations and aberrant expression in DNMT genes are found in multiple human tumors. The discovery of the DNA demethylation function of TET proteins has open...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-29.991
更新日期：2014-08-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-11-718
更新日期：2016-06-01 00:00:00
abstract：BACKGROUND:Little is known regarding the role of redox balance regulators in metastatic melanomas, but there is some evidence for a link between epithelial-to-mesenchymal transition (EMT) and cellular redox status. METHODS:We compared the immunohistochemical expression of nuclear factor erythroid-2-related factor 2 (N...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-11-778
更新日期：2017-02-01 00:00:00
abstract：:In recent years, the different mechanisms of epithelial cell loss which occur in mammalian and avian small intestine have been re-investigated. Information is now available for a variety of mammalian types and mechanisms can be divided into two major classes: [i] those preserving epithelial integrity by maintaining in...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-14.257
更新日期：1999-01-01 00:00:00
abstract：:Ischemic heart disease is a widespread cause of death. During infarction, myocardial injury is mediated by release of several pro-inflammatory cytokines including multifunctional interleukin-1 (IL-1). In various tissues, IL-1-mediated deleterious effects are known to be attenuated via the over-expression of natural an...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-23.1093
更新日期：2008-09-01 00:00:00
abstract：:Primary perivascular epithelioid cell tumor (PEComa) of the liver is a very rare example of an emerging family of hepatic PEC tumors. Only few cases have been described so far. We report the case of a large but benign hepatic PEComa in a 53-year-old man without signs of tuberous sclerosis. In contrast to recently desc...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-23.1185
更新日期：2008-10-01 00:00:00
abstract：:Various microscopic methods have been used to analyze the morphology and molecular distribution of cells and tissues. Using conventional procedures, however, ischemic or anoxic artifacts are inevitably caused by tissue-resection or perfusion-fixation. The in vivo cryotechnique (IVCT) was developed to overcome these pr...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-22.1281
更新日期：2007-11-01 00:00:00
abstract：BACKGROUND:Gastric Cardiac Cancer (GCC) has high incidence and poor prognosis requiring early screening of high-risk populations. Minichromosome maintenance (MCM) proteins are used as diagnostic-biomarkers in many cancers but not validated for GCC. We evaluate MCM protein 2 (MCM2), comparing it with the validated marke...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-28.481
更新日期：2013-04-01 00:00:00
abstract：:We examined the relationship between the incidence of ultrastructural changes in the alveolar septum and the extravascular lung water content. Pulmonary edema was induced in 18 mongrel dogs by either dextran (n = 12) or alloxan (n = 6) administration. Six other dogs served as controls. Extravascular lung water content...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
