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Isolation of rat adrenocortical mitochondria.

Abstract：

:This report describes a relatively simple and reliable method for isolating adrenocortical mitochondria from rats in good, reasonably pure yield. These organelles, which heretofore have been unobtainable in isolated form from small laboratory animals, are now readily accessible. A high degree of mitochondrial purity is shown by the electron micrographs, as well as the structural integrity of each mitochondrion. That these organelles have retained their functional integrity is shown by their high respiratory control ratios. In general, the biochemical performance of these adrenal cortical mitochondria closely mirrors that of typical hepatic or cardiac mitochondria.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Solinas P,Fujioka H,Tandler B,Hoppel CL

doi

10.1016/j.bbrc.2012.09.014

subject

Has Abstract

pub_date

2012-10-12 00:00:00

pages

96-9

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(12)01741-X

journal_volume

427

pub_type

杂志文章

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