Abstract：
:cDNA encoding VEGF and Ig-like extracellular domains 2-4 of VEGFR-1 (sFlt-1(2-4)) were cloned into prokaryotic expression vectors pET32a and pQE60. Recombinant proteins were purified (metal affinity chromatography) and renatured. Chemiluminescent study for the interaction of recombinant VEGF and sFlt-1(2-4) showed that biotinylated VEGF specifically binds to the polystyrene-immobilized receptor extracellular fragment. Biotinylated recombinant sFlt-1 interacts with immobilized VEGF. Analysis of the interaction of immobilized recombinant VEGFR-1 and VEGF with C6 glioma cells labeled with CFDA-SE (vital fluorescent dye) showed that recombinant VEGFR-1 also binds to native membrane-associated VEGF. Recombinant VEGF was shown to bind to specific receptors expressed on the surface of C6 glioma cells. Functional activity of these proteins was confirmed by ligand-receptor assay for VEGF and VEGFR-1 (sFlt-1) and quantitative chemiluminescent detection.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Leopol'd AV,Baklaushev VP,Korchagina AA,Shein SA,Grinenko NF,Pavlov KA,Ryabukhin IA,Chekhonin VPdoi
10.1007/s10517-012-1612-0subject
Has Abstractpub_date
2012-04-01 00:00:00pages
707-11issue
6eissn
0007-4888issn
1573-8221journal_volume
152pub_type
杂志文章abstract：:During left-ventricular myocardial ischemia, the decrease in the venous return is determined by reduction of cardiac output, which results from heart rate deceleration and decrease in left-ventricular stroke volume. The latter is related to a decrease in myocardial contractility and blood storage in the pulmonary circ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-012-1542-x
更新日期：2012-02-01 00:00:00
abstract：:Effects of local therapy with miacalcic on the metabolism in articular connective tissue structures under conditions of experimental arthritis are shown. An effect modifying articular tissue metabolism and an antiinflammatory effect preventing the development of inflammatory destructive processes in rabbit articular t...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-007-0108-9
更新日期：2007-03-01 00:00:00
abstract：:The content of hemopoietic precursors in the bone marrow, its morphological composition, production of hemopoietic growth factors by cells of the hemopoietic microenvironment, erythropoietic activity, and plasma content of endogenous erythropoietin were studied in CBA/CaLac mice with hypoplasia of hemopoiesis induced ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/a:1025469000276
更新日期：2003-06-01 00:00:00
abstract：:Lyzed epimastigotes of Trypanosoma cruzi clones P209-1, Gamba1, Sp104-1, MASu, Y7/1, MN12, Cl-Brener, 86/2036, Y7/2-1 inhibit the growth of Ehrlich adenocarcinoma in mice. the tumor decreased 1.5-3 times after 12 daily injections of lysates from 15 million epimastigotes. The protective effect progressed after the inje...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-006-0395-6
更新日期：2006-10-01 00:00:00
abstract：:The effects of ladasten and reference product imipramine (10 mg/kg) on the content of cytokines TNF-α, IL-6, IL-10, IL-1α, IL-2, IL-4, IL-5, IL-17, IFN-γ, and GM-CSF and behavior of male C57Bl/6 mice were studied on the model of a depression-like state induced by a single intraperitoneal injection of bacterial LPS in ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-011-1453-2
更新日期：2011-11-01 00:00:00
abstract：:The effects of two derivatives of 2-aminoadamantane, enantiomers J447H and J579, on the behavior of male and female C57Bl/6J mice were studied using a modified light/dark test. The substances differed by their effects on the behavior of male mice. J579 reduced the number of rearings. J447H in a dose of 1 mg/kg affecte...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-014-2725-4
更新日期：2014-12-01 00:00:00
abstract：:The content of phospholipids in various tissues was studied during synergistic interaction between two branches of the autonomic nervous system. We found higher myocardial content of choline-containing phospholipids and lower rigidity of erythrocyte membranes in rabbits with pronounced sympathetic potentiation of the ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/a:1016203713719
更新日期：2002-04-01 00:00:00
abstract：:Acute diphtheritic intoxication was modeled in rabbits by intravenous administration of native diphtherin (0.3 minimal lethal dose per 1 kg body weight). The contractile function of the left and right ventricles (peak systolic pressure under conditions of basal hemodynamics and during 5-sec occlusion of the aorta and ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-010-0714-9
更新日期：2009-09-01 00:00:00
abstract：:The effects of Ro 25-6981 (selective NMDA receptor blocker) in a dose stimulating neurogenesis on repeated learning, reversal learning, and memory reconsolidation were studied in adult rats in Morris water maze. Ro 25-6981 facilitated repeated learning 13 days after injection, but did not influence reversal learning. ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-012-1821-6
更新日期：2012-09-01 00:00:00
abstract：:Preparation containing ultralow doses of antibodies to stem cell factor considerably activates bone marrow myelopoiesis suppressed by cyclophosphamide. This effect of the preparation is based on stimulation of proliferation of committed hemopoietic precursors and increase in functional activity of adherent elements of...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-010-0764-z
更新日期：2009-09-01 00:00:00
abstract：:The effects of cerivastatin on antiatherogenic properties of high-density lipoproteins were studied in patients with coronary heart disease and hyperlipidemia. Apart from hypolipidemic effects, cerivastatin changed the phospholipid composition of high-density lipoproteins and improved their cholesterol-acceptor proper...
journal_title：Bulletin of experimental biology and medicine
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：2000-10-01 00:00:00
abstract：:Experiments on rats showed that blockade of hyperpolarization-activated currents moderates tachycardia induced by beta-adrenoceptor agonist isoproterenol and potentiates the increase in stroke volume produced by this agonist. Electrical stimulation of the vagus nerve against the background of isoproterenol treatment a...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/a:1019884931598
更新日期：2002-05-01 00:00:00
abstract：:Study of the expression of type IV collagen by Lewis pulmonary carcinoma cells (PLMG-T, PLMG-M1, PLMG-M2) showed that these cells produce type IV collagen. The expression of type IV collagen in PLMG-T and PLMG-M2 cells approximately 2-fold surpassed that in PLMG-M1 cells. The number of active cells (synthesizing type ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/b:bebm.0000024390.08829.ee
更新日期：2004-01-01 00:00:00
abstract：:Differential expression of type 1 cannabinoid receptors (CR1) was evaluated at different stages of human skin fibroblast transformation into terminally differentiated neurons. Immunocytochemical staining detected no CR1 on fibroblasts, but their transformation into induced pluripotent stem cells was accompanied by mar...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-017-3782-2
更新日期：2017-06-01 00:00:00
abstract：:We studied the effect of constant illumination for 1 month on female GC (genetic catalepsy) and Wistar rats (control). Light exposure decreased the degree of cataleptic freezing in female GC rats (compared to the control). Norepinephrine concentration in cataleptic rats returned to normal under these conditions: this ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-008-0200-9
更新日期：2008-07-01 00:00:00
abstract：:The age-related features of ultrastructural reorganization of cardiomyocytes were studied in rats with anthracycline-induced injury. The development of regenerative and plastic insufficiency of cardiomyocytes in animals of various age groups was accompanied by stereotypic ultrastructural reorganization. The major chan...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-011-1266-3
更新日期：2011-05-01 00:00:00
abstract：:A liposomal preparation with maximally possible content of incorporated geliomycin was obtained. Its cytotoxicity was studied in a culture of human embryonal diploid fibroblasts. Antiviral activity was studied on a model of cytomegaloviral infection in vitro by the capacity to plaque formation. Liposomal geliomycin wa...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-005-0291-5
更新日期：2005-03-01 00:00:00
abstract：:Precise location of IL-18 in cell and tissue elements of the atherosclerotic lesions in humans and its role in destabilization of the atherosclerotic plaque were detected. The data suggested a hypothesis on indirect involvement of IL-18 in destruction of the elastic and collagen fibers in an unstable plaque due to thi...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-014-2675-x
更新日期：2014-10-01 00:00:00
abstract：:We studied the effect of audiovisual stimulation training course on physical development, functional state of the cardiovascular system, blood biochemical parameters, and hormonal status of athletes. The training course led to improvement of physical performance and adaptive capacities of the circulatory system, incre...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-016-3474-3
更新日期：2016-09-01 00:00:00
abstract：:We studied the role of DNA methylation in the mechanisms of amnesia in edible snails, which was induced by impairment of conditioned food aversion memory reconsolidation with NMDA glutamate receptor antagonist. The effects of DNA methyltransferase inhibitors were shown to depend on the stage of amnesia. At the early s...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-014-2367-6
更新日期：2014-02-01 00:00:00
abstract：:Here we present the results of histochemical detection of 3β-hydroxysteroid dehydrogenase in neurons of the neocortex, cerebellum, and brainstem. The positive reaction was observed in a low number of neocortical, midbrain, and pontine neurons. The maximum number of positive neurons was detected in the cerebellar corte...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-012-1532-z
更新日期：2012-01-01 00:00:00
abstract：:Clinical and laboratory studies were carried out in 38 pregnant women with antiphospholipid syndrome. Increased functional activity of platelets and decreased protein-producing function of the placenta were observed starting from the early terms of gestation. These disorders were followed by the development of hyperco...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/a:1021928903012
更新日期：2002-10-01 00:00:00
abstract：:Single intramuscular injection of selective of NMDA receptor blocker memantine in the maximum dose of 20 mg/kg prevented the development of acute generalized tonic-clonic kainate seizures in 60% rats, but did not alleviate clonic kainate seizures and prevented chronic kainate lethality in only 30% rats. Intramuscular ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-014-2480-6
更新日期：2014-05-01 00:00:00
abstract：:Enzyme immunoassays showed significantly elevated content of matrix metalloproteinase 7 and type 1 tissue inhibitor of metalloproteinases in tumors compared to adjacent histologically unchanged mucosa of patients with colorectal cancer; the levels of metalloproteinase 9 and type 4 tissue inhibitor of metalloproteinase...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-007-0156-1
更新日期：2007-04-01 00:00:00
abstract：:We developed a multiplexed DNA microarray-based assay allowing identification of 12 causative agents of reproductive tract infections with the simultaneous detection of 47 genetic determinants of resistance to antimicrobial substances. The microarray was tested on 93 isolates of Neisseria gonorrhoeae, 32 isolates of T...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-017-3925-5
更新日期：2017-11-01 00:00:00
abstract：:More than 70% malignant mammary tumors contain steroid hormone receptors; this suggests the possibility of hormone therapy in the majority of patients with breast cancer (BC). The main cause of inefficiency of hormone therapy in BC is hormone resistance (tumor resistance to hormonal cytostatics). Here we discuss the m...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-013-2160-y
更新日期：2013-07-01 00:00:00
abstract：:The content of low-molecular-weight components in blood serum was studied by tandem mass-spectrometry in pregnant women. Serum metabolic profiles of patients with a grave obstetrical history were detected. The most significant changes were observed for the concentrations of low-molecular-weight substances involved in ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-013-2287-x
更新日期：2013-11-01 00:00:00
abstract：:The relationship between the process of ethanol preference formation in rat pups periodically separated from mothers during the first half of the nest period and the intensity of maternal behavior of females was studied. The progeny of females with poor maternal behavior and/or separated from mothers is characterized ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-009-0533-z
更新日期：2009-04-01 00:00:00
abstract：:We compared the level of transforming growth factor-β1 (TGF-β1) in the tear fluid of 20 patients with high myopia who underwent transepithelial photorefractive keratectomy (T-PRK) in one eye and laser subepithelial keratomileusis (LASEK) in the contralateral eye. Tear fluid samples were collected with scaled microcapi...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-020-05008-z
更新日期：2020-11-01 00:00:00
abstract：:We studied the interaction of neural stem cells and dental pulp-derived mesenchymal stem cells with lymphocytes from autologous and heterologous donors. Flow cytometry analysis with the use of CFSE-labeled lymphocytes demonstrated an increase in the content of proliferating CD8, CD16 and CD56 cells, but not CD4 cells ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-016-3463-6
更新日期：2016-08-01 00:00:00
