Abstract:
:The basic helix-loop-helix (bHLH) transcription factor Ascl1 plays crucial roles in both oligodendrocyte development and neuronal development; however, the molecular target of Ascl1 in oligodendrocyte progenitor cells (OPCs) remains elusive. To identify the downstream targets of Ascl1 in OPCs, we performed gene expression microarray analysis and identified Hes5 as a putative downstream target of Ascl1. In vivo analysis revealed that Ascl1 and Hes5 were coexpressed in early developmental oligodendrocytes in both the telencephalon and the ventral spinal cord. We also found that Hes5 expression was reduced in the OPCs of Ascl1 mutant mice. Furthermore, we demonstrated that Ascl1 directly binds to an E-box region within the Hes5 promoter and regulates Hes5 expression at the transcriptional level. Taken together, these in vivo and in vitro data suggest that Ascl1 induces Hes5 expression in a cell-autonomous manner. Considering the previously known function of Hes5 as a repressor of Ascl1, our data indicate that Hes5 is involved in the negative feedback regulation of Ascl1.
journal_name
Gliajournal_title
Gliaauthors
Ueno T,Ito J,Hoshikawa S,Ohori Y,Fujiwara S,Yamamoto S,Ohtsuka T,Kageyama R,Akai M,Nakamura K,Ogata Tdoi
10.1002/glia.22369subject
Has Abstractpub_date
2012-10-01 00:00:00pages
1495-505issue
10eissn
0894-1491issn
1098-1136journal_volume
60pub_type
杂志文章相关文献
GLIA文献大全abstract::The technology to generate autologous pluripotent stem cells (iPS cells) from almost any somatic cell type has brought various cell replacement therapies within clinical research. Besides the challenge to optimize iPS protocols to appropriate safety and GMP levels, procedures need to be developed to differentiate iPS ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.21159
更新日期:2011-06-01 00:00:00
abstract::We have attempted to reconstruct in vitro the events that may occur in vivo during reperfusion injury after ischemia in the central nervous system. The phenomenon is induced by previous exposure to low calcium solutions ("calcium paradox") before the reperfusion episode. Intracellular calcium alterations during reperf...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440050109
更新日期:1992-01-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder affecting predominantly motor neurons. Recent studies suggest that the disease progression of ALS is non-cell-autonomous, although the interaction between neurons and glial cells in different disease stages is not entirely clear. Here, we ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.21167
更新日期:2011-06-01 00:00:00
abstract::A major role for Müller cells in the retina is to buffer changes in the extracellular K+ concentration ([K+]o) resulting from light-evoked neuronal activity. The primary K+ conductance in Müller cells is the inwardly rectifying K+ channel Kir4.1. Since this channel is constitutively active, K+ can enter or exit Müller...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20271
更新日期:2006-01-15 00:00:00
abstract::Retinoic acid (RA), a metabolite of vitamin A, is required for the regulation of growth and development. Aberrant expression of molecules involved in RA signaling has been reported in various cancer types including glioblastoma multiforme (GBM). Cellular retinoic acid-binding protein 2 (CRABP2) has previously been sho...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22976
更新日期:2016-06-01 00:00:00
abstract::Mouse sciatic nerves from the degeneration-resistant strain C57BL/6/Wld (Ola) were surgically injected with lysolecithin to induce focal demyelination. Three days later they were transected adjacent to the spinal cord to eliminate contact of the axons with their cell bodies. The Na+ channel distribution was assessed b...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440150211
更新日期:1995-10-01 00:00:00
abstract::Oligodendroglia and Schwann cells synthesize myelin-specific proteins and lipids for the assembly of the highly organized myelin membrane of the motor-sensory axons in the central (CNS) and peripheral nervous system (PNS), respectively, allowing rapid saltatory conduction. The isoforms of the main myelin proteins, the...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.10014
更新日期:2002-01-01 00:00:00
abstract::A role for free radicals has been proposed in infectious brain disease, where resident microglia cells upregulate the inducible nitric oxide synthase isoform (iNOS), and thus are capable of producing nitric oxide at enhanced rates. Using the constitutively expressed NADPH oxidase, microglial cells can generate superox...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/1098-1136(200010)32:1<51::aid-glia50>3.0.c
更新日期:2000-10-01 00:00:00
abstract::We have previously shown that cultured astrocytes from neonatal rat cerebral cortex are depolarized by GABA. The underlying ionic mechanism, activation of a Cl- conductance and responses to an agonist and antagonists were found to be similar to those of the neuronal GABAA receptor (Kettenmann et al.: Brain Research 40...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440010205
更新日期:1988-01-01 00:00:00
abstract::The X-linked mutation rumpshaker (rsh), which is probably an allele of jimpy (jp), causes hypomyelination in the CNS of mice. This study examines the developmental expression of the morphology, glial cells, and immunostaining of myelin proteins in the optic nerve and spinal cord. The optic nerve contains varying numbe...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440050302
更新日期:1992-01-01 00:00:00
abstract::Differentiation of oligodendrocyte precursor cells (OPCs) is the most important event for the myelination of central nervous system (CNS) axons during development and remyelination in demyelinating diseases, while the underlying molecular mechanisms remain largely unknown. Here we show that NMDA receptor (NMDAR) is a ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22469
更新日期:2013-05-01 00:00:00
abstract::Zinc finger transcription factors of the Krüppel-class figure prominently in cell fate specification and differentiation in the nervous system. One of the Krüppel-type genes that was originally cloned from an oligodendrocyte library by virtue of its homology with the prototypic Krüppel motif is the rat rKr2 gene (Pott...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.1046
更新日期:2001-04-15 00:00:00
abstract::Tumor necrosis factor (TNF) and its receptors TNFR1 and TNFR2 have pleiotropic effects in neurodegenerative disorders. For example, while TNFR1 mediates neurodegenerative effects in multiple sclerosis, TNFR2 is protective and contributes to remyelination. The exact mode of TNFR2 action, however, is poorly understood. ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22605
更新日期:2014-02-01 00:00:00
abstract::Cell death in the oligodendrocyte lineage occurs during development and in pathological conditions as the result of a balance between opposing molecular signals. This review focuses on the molecular mechanisms of activation of signal transduction pathways affecting life/death decisions in progenitor cells and in matur...
journal_title:Glia
pub_type: 杂志文章,评审
doi:10.1002/(sici)1098-1136(20000115)29:2<124::aid-gli
更新日期:2000-01-15 00:00:00
abstract::To investigate the possible role of neurons and astrocytes for oligodendrocyte development we prepared a pure population of precursor cells positive for the precursor marker GD3 with the help of fluorescence-activated cell sorting (FACS). Large numbers of highly purified cells were obtained from postnatal day 1 rat br...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440040603
更新日期:1991-01-01 00:00:00
abstract::Allograft inflammatory factor-1 (AIF-1) is a Ca2+ binding peptide expressed predominantly by activated monocytes. In order to investigate the role of AIF-1 in autoimmune lesions of the rat nervous system, we have used a synthetic gene to express AIF-1 in E. coli and have produced monoclonal antibodies against AIF-1. A...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/(sici)1098-1136(199810)24:2<244::aid-glia9
更新日期:1998-10-01 00:00:00
abstract::In order to improve understanding of remodeling events in the glaucomatous optic nerve head, the migration of optic nerve head astrocytes was studied in vitro. Since elevated intraocular pressure is an important stress factor identified in glaucomatous eyes, optic nerve head astrocytes were incubated under physical st...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.1052
更新日期:2001-05-01 00:00:00
abstract::Our previous studies demonstrated the involvement of quinone formation in dopaminergic neuron dysfunction in the L-DOPA-treated parkinsonian model and in methamphetamine (METH) neurotoxicity. We further reported that the cysteine-rich metal-binding metallothionein (MT) family of proteins protects dopaminergic neurons ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.21112
更新日期:2011-03-01 00:00:00
abstract::Regeneration in the adult central nervous system (CNS) is thought to be hampered by the lesion-induced activation of astrocytes and meningeal cells and the consecutive formation of a glial scar. The substrate properties of reactive astrocytes differ significantly from their neonatal counterparts, which promote axon gr...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/(sici)1098-1136(199903)26:1<36::aid-glia4>
更新日期:1999-03-01 00:00:00
abstract::We have previously reported that neonatal rat oligodendrocytes (OLGs) express and secrete neuregulins (NRGs) (Raabe et al., 1997). This laboratory has also shown that NRGs stimulate the differentiation of neonatal rat OLGs and that these cells express the erbB receptors for NRGs (Raabe et al., 1997). In this study, we...
journal_title:Glia
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::An organism's response to stress requires activation of multiple brain regions. This can have long-lasting effects on synaptic transmission and plasticity that likely provide adaptive benefits. Recent evidence implicates not only neurones, but also glial cells in the regulation of the central response to stress. Inten...
journal_title:Glia
pub_type: 杂志文章,评审
doi:10.1002/glia.23610
更新日期:2019-10-01 00:00:00
abstract::In the course of adult hippocampal neurogenesis, new cells go through a series of stages associated with proliferative activity. The most highly proliferative cell type is an intermediate precursor cell, called type-2 cell. We here report that on the level of type-2 cells a transition takes place between features asso...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20407
更新日期:2006-12-01 00:00:00
abstract::The role of microglia in retinal inflammation is still ambiguous. Branch retinal vein occlusion initiates an inflammatory response whereby resident microglia cells are activated. They trigger infiltration of neutrophils that exacerbate blood-retina barrier damage, regulate postischemic inflammation and irreversible lo...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23739
更新日期:2020-03-01 00:00:00
abstract::Serum is used widely for culturing neurons and glial cells, and is thought to provide essential, albeit undefined, factors such as hormones, growth factors, and trace elements that promote the growth of cells in vitro. Moreover, serum can have profound effects on cell proliferation, differentiation, and cell morpholog...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/(sici)1098-1136(199803)22:3<237::aid-glia3
更新日期:1998-03-01 00:00:00
abstract::Extracellular purines are signaling molecules in the neurogenic niches of the brain and spinal cord, where they activate cell surface purinoceptors at embryonic neural stem cells (NSCs) and adult neural progenitor cells (NPCs). Although mRNA and protein are expressed at NSCs/NPCs for almost all subtypes of the nucleot...
journal_title:Glia
pub_type: 杂志文章,评审
doi:10.1002/glia.23056
更新日期:2017-02-01 00:00:00
abstract::Theiler's murine encephalomyelitis virus (TMEV) is known to interact with cells of the central nervous system (CNS). Here we report that, interestingly, it is a potent inductor of interleukin-6 (IL-6) in the CNS of infected animals and in pure cultures of astrocytes. Maximal IL-6 gene transcription in glial cells, as ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440090106
更新日期:1993-09-01 00:00:00
abstract::Oligodendroglial cells undergo rapid transcriptional and dynamic morphological transformation in order to effectively myelinate neuronal axons. Olig1, a basic helix-loop-helix transcription factor, functions to promote the transcription of myelin-specific genes and promotes differentiation and (re)myelination. While t...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22364
更新日期:2012-09-01 00:00:00
abstract::Gliomas are a heterogenous group of malignant primary brain tumors that arise from glia cells or their progenitors and rely on accurate diagnosis for prognosis and treatment strategies. Although recent developments in the molecular biology of glioma have improved diagnosis, classical histological methods and biomarker...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23594
更新日期:2019-08-01 00:00:00
abstract::Microglia respond rapidly to injury, increasing their synthesis and release of inflammatory mediators, many of which contribute to the maintenance of persistent pain following CNS or PNS injury. We have recently shown that the lysosomal cysteine protease Cathepsin S (CatS) expressed by spinal microglia is vital for th...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.21042
更新日期:2010-11-01 00:00:00
abstract::The production of insulin-like growth factor (IGF)-I and -II and their binding proteins (BPs) has been studied in new-born rat astroblasts at confluency in primary culture. Under the influence of fibroblast growth factors (FGFs) (acidic and basic), the morphology of the astroblasts was altered, 125I-deoxyuridine incor...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440040405
更新日期:1991-01-01 00:00:00