Abstract:
:To investigate the possible role of neurons and astrocytes for oligodendrocyte development we prepared a pure population of precursor cells positive for the precursor marker GD3 with the help of fluorescence-activated cell sorting (FACS). Large numbers of highly purified cells were obtained from postnatal day 1 rat brainstems and cultured in different media and sera, and in conditioned media. As described in the literature for optic nerve O-2A progenitors, GD3-sorted brainstem cells cultured in medium containing 10% fetal calf serum (FCS) acquired a star-shaped morphology and differentiated into GD3- and GFAP-positive type-2 astrocytes. On the other hand, in serum-free medium, most of the cells differentiated into oligodendrocytes (O1-/galactocerebroside-positive). Sensory neuron conditioned media promoted survival and proliferation of the precursor cells. The spontaneous differentiation of progenitor cells into oligodendrocytes was retarded by the mitogen. Antibodies against platelet-derived growth factor (PDGF) completely blocked the mitotic effect and allowed spontaneous oligodendrocyte differentiation to occur. Cultured astrocytes also secreted PDGF as a mitogen. However, postnatal astrocytes also released a potent signal promoting oligodendrocyte differentiation. The type of factor(s) released depended on the age of the astrocytes, since only conditioned medium of postnatal but not of embryonic astrocytes promoted oligodendrocyte differentiation, suggesting that astrocyte maturation directly influences oligodendrocyte differentiation. Different concentrations of PDGF could not reproduce this differentiation-inducing effect. This study suggests that interactions between O-2A progenitor cells, neurons, and astrocytes could be required to regulate and complete the oligodendrocyte developmental pathway. Astrocytes, themselves possibly under neuronal influences, might regulate first the proliferation of the precursor cells, and, later in development, the differentiation into mature oligodendrocytes or type-2 astrocytes.
journal_name
Gliajournal_title
Gliaauthors
Dutly F,Schwab MEdoi
10.1002/glia.440040603subject
Has Abstractpub_date
1991-01-01 00:00:00pages
559-71issue
6eissn
0894-1491issn
1098-1136journal_volume
4pub_type
杂志文章相关文献
GLIA文献大全abstract::Sulfated glycosaminoglycans (S-GAGs) were isolated from the pericellular (P), intracellular (I), and extracellular (E) compartments of astrocytes cultures from lateral (L) and medial (M) sectors of embryonic mouse midbrain; these sectors differ in their ability to support neurite growth (L, permissive, M, non-permissi...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/(SICI)1098-1136(199608)17:4<339::AID-GLIA8
更新日期:1996-08-01 00:00:00
abstract::Microglia are the predominant resident immune cells of the brain and can assume a range of phenotypes. They are critical for normal brain development and function but can also contribute to many disease processes. Although they are widely studied, the transcriptional control of microglial phenotype and activation requ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22486
更新日期:2013-06-01 00:00:00
abstract::The removal by phagocytosis of degenerated myelin is central for repair in Wallerian degeneration that follows traumatic injury to axons and in autoimmune demyelinating diseases (e.g., multiple sclerosis). We tested for roles played by the cAMP cascade in the regulation of myelin phagocytosis mediated by complement re...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20303
更新日期:2006-03-01 00:00:00
abstract::To develop effective procedures for improving the regeneration of peripheral nerves and for preventing the formation of neurofibromas, it is necessary to identify the different mitogens that stimulate the proliferation of Schwann cells. Insulin-like growth factor I (IGF-I), which is a potent autocrine growth factor in...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440080403
更新日期:1993-08-01 00:00:00
abstract::Microglial cells are the resident macrophages that are involved in brain injuries and infections. Recent studies using transcranial two-photon microscopy have shown that ATP and P2Y receptors mediated rapid chemotactic responses of miroglia to local injury. However, the molecular mechanism for microglial chemotaxis to...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20500
更新日期:2007-06-01 00:00:00
abstract::Neuroprotective M2-skewed microglia appear as promising to alter the course of neurodegenerative diseases and G protein-coupled receptors (GPCRs) are potential targets to achieve such microglial polarization. A common feature of adenosine A2A (A2A R) and cannabinoid CB2 (CB2 R) GPCRs in microglia is that their express...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23694
更新日期:2019-12-01 00:00:00
abstract::Mechanisms of major histocompatibility complex (MHC) class I gene regulation in cells of the CNS have been studied in vitro. Astrocytes in primary cultures, but neither oligodendrocytes nor neurons, constitutively expressed cell surface MHC class I molecules. Interferon-gamma (IFN-gamma) treatment led to induction of ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440080307
更新日期:1993-07-01 00:00:00
abstract::The extrasynaptic region of the squid giant nerve fiber exhibits neuron-Schwann cell interactions that appear to involve glutamate as a mediator. In an earlier work, it was demonstrated that the periaxonal sheaths of such nerves (where the Schwann cells are located) possess the capacity to transport glutamate. However...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/(sici)1098-1136(199808)23:4<298::aid-glia2
更新日期:1998-08-01 00:00:00
abstract::These studies have enabled the first characterization of the properties of ion channels in adult oligodendrocytes. Cell-attached recordings from cultured adult bovine cells showed channel activity with 140 mM KCl in the patch pipette; the amplitude of the currents was increased with increasing membrane hyperpolarizati...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440020503
更新日期:1989-01-01 00:00:00
abstract::Niemann-Pick disease type C (NPC) is a deadly neurodegenerative disease often caused by mutation in a gene called NPC1, which results in the accumulation of unesterified cholesterol and glycosphingolipids in the endosomal-lysosomal system. Most studies on the mechanisms of neurodegeneration in NPC have focused on neur...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20563
更新日期:2007-11-15 00:00:00
abstract::Oligodendrocyte precursor cells (OPCs) originate from restricted regions of the brain and migrate into the developing white matter, where they differentiate into oligodendrocytes and myelinate axons in the central nervous system (CNS). The molecular mechanisms that orchestrate these long distance trips of OPCs to popu...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22578
更新日期:2013-12-01 00:00:00
abstract::GABA receptors are distributed widely throughout the central nervous system on a variety of cell types. It has become increasingly clear that astrocytes, both in cell culture and tissue slices, express abundant GABAA receptors. In astrocytes, GABA activates Cl(-)-specific channels that are modulated by barbiturates an...
journal_title:Glia
pub_type: 杂志文章,评审
doi:10.1002/glia.440110203
更新日期:1994-06-01 00:00:00
abstract::Pax-3 is a paired domain transcription factor that plays many roles during vertebrate development. In the Schwann cell lineage, Pax-3 is expressed at an early stage in Schwann cells precursors of the embryonic nerve, is maintained in the nonmyelinating cells of the adult nerve, and is upregulated in Schwann cells afte...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22346
更新日期:2012-09-01 00:00:00
abstract::Apart from dedicated oligodendroglial progenitor cells, adult neural stem cells (aNSCs) can also give rise to new oligodendrocytes in the adult central nervous system (CNS). This process mainly confers myelinating glial cell replacement in pathological situations and can hence contribute to glial heterogeneity. Our pr...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23724
更新日期:2020-02-01 00:00:00
abstract::Axonal signals activate myelinogenesis via regulation of the extent to which oligodendrocyte (OLG) processes wrap around the axon. The cytoskeleton in OLG processes is actively involved in myelination and is a putative target for axonal regulation of myelination. The axon-associated neuregulins may regulate the cytosk...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.1079
更新日期:2001-08-01 00:00:00
abstract::We have shown previously that switching of the alpha v-associated beta1 and beta5 integrin subunits during differentiation of myelin-forming oligodendrocytes may regulate important aspects of cell behaviour such as migration (Milner et al., 1996: J Neurosci 16:7240-7252). In this study we have examined the development...
journal_title:Glia
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract::Superoxide dismutase (SOD) is considered to be a major factor in protection of nervous tissue against excitotoxic and ischemic/hypoxic lesion. Controversial reports about the localization of SOD after such an insult prompted us to re-investigate immunocytochemically the localization of the enzyme in the brain and spin...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/(sici)1098-1136(20000101)29:1<25::aid-glia
更新日期:2000-01-01 00:00:00
abstract::Herpes simplex virus Type I (HSV-1) is a neurotropic virus that is capable of infecting not only neurons, but also microglia and astrocytes and can establish latent infection in the central nervous system (CNS). We investigated whether IFN lambda (IFN-λ), a newly identified member of IFN family, has the ability to inh...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.21076
更新日期:2011-01-01 00:00:00
abstract::Individuals with neurofibromatosis 1 (NF1) develop low-grade astrocytomas at an increased frequency. To gain insight into the function of the Nf1 gene product as a growth regulator for astrocytes, we examined mice heterozygous for a targeted Nf1 mutation. In our previous studies, we demonstrated increased numbers of p...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/1098-1136(20010315)33:4<314::aid-glia1030>
更新日期:2001-03-15 00:00:00
abstract::Extracellular levels of the brain's endogenous anticonvulsant and neuroprotectant adenosine largely depend on an astrocyte-based adenosine cycle, comprised of ATP release, rapid degradation of ATP into adenosine, and metabolic reuptake of adenosine through equilibrative nucleoside transporters and phosphorylation by a...
journal_title:Glia
pub_type: 杂志文章,评审
doi:10.1002/glia.22285
更新日期:2012-08-01 00:00:00
abstract::Oligodendrocytes, the myelinating cells of the CNS, are derived postnatally from oligodendrocyte precursors (OPs) of the subventricular zone (SVZ). However, the mechanisms that regulate their generation from SVZ neural stem cells (NSC) are poorly understood. Here, we have examined the role of glycogen synthase kinase ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22641
更新日期:2014-05-01 00:00:00
abstract::The creatine kinase isoenzymes play an important role in maintaining ATP levels in some cell types during times of high energy demand. We have previously shown in primary cell cultures from rat brain that glial cells express much higher levels of brain creatine kinase (CKB) mRNA than neurons. In a separate earlier stu...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440150410
更新日期:1995-12-01 00:00:00
abstract::We have previously shown that cultured astrocytes from neonatal rat cerebral cortex are depolarized by GABA. The underlying ionic mechanism, activation of a Cl- conductance and responses to an agonist and antagonists were found to be similar to those of the neuronal GABAA receptor (Kettenmann et al.: Brain Research 40...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440010205
更新日期:1988-01-01 00:00:00
abstract::Minocycline, a derivative of the antibiotic tetracycline, displays neuroprotective properties in various models of neurodegenerative diseases and is now used in clinical trials, because of its relative safety and tolerability. Minocycline passes the blood-brain barrier and is presumed to inhibit microglial activation....
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20268
更新日期:2006-02-01 00:00:00
abstract::The possibility of a modular organization of non-neuronal elements was analyzed in the opercular region of the striate neocortex in adult New World monkeys. For this purpose, and in order to follow possible correlations in the general organization of neuronal and astroglial elements, immunocytochemical procedures for ...
journal_title:Glia
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Enriched preparations of oligodendrocytes, isolated either from adult bovine brain or from 30-day-old rat brain, eventually yield cultures in MEM-15% calf serum that contain, in addition to oligodendrocytes, proliferating astrocytes and variable numbers of fibroblast-like cells. If these cultures are switched to a ser...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440010608
更新日期:1988-01-01 00:00:00
abstract::Glial cells in vitro express at least two types (H1 and H2) of histamine receptors and three types (EP, FP, and TP) of prostanoid receptors. The receptors expressed by glial cells differ according to the cell type and source in the brain. Furthermore primary astrocytes of same type derived from the same brain region a...
journal_title:Glia
pub_type: 杂志文章,评审
doi:10.1002/glia.440110205
更新日期:1994-06-01 00:00:00
abstract::Cancer stem cells (CSC) are essential for tumorigenesis. The transcription factor Sox2 is overexpressed in brain gliomas, and is essential to maintain CSC. In mouse high-grade glioma pHGG cells in culture, Sox2 deletion causes cell proliferation arrest and inability to reform tumors after transplantation in vivo; in S...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23914
更新日期:2021-03-01 00:00:00
abstract::Microglia have diverse actions, ranging from synapse pruning in development to cytotoxic effects in disease. Brain energy metabolism and substrate availability vary under normal and disease states, but how these variations influence microglial function is relatively unknown. Microglia, like most other cell types, expr...
journal_title:Glia
pub_type: 杂志文章,评审
doi:10.1002/glia.23271
更新日期:2018-06-01 00:00:00
abstract::There is a critical relationship between oligodendrocyte development, myelin production, and iron bioavailability. Iron deficiency leads to hypomyelination both in humans and animal models, and the neurological sequelae of hypomyelination are significant. Therefore, understanding molecular mechanisms of iron import in...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.21164
更新日期:2011-06-01 00:00:00