Abstract:
:Superoxide dismutase (SOD) is considered to be a major factor in protection of nervous tissue against excitotoxic and ischemic/hypoxic lesion. Controversial reports about the localization of SOD after such an insult prompted us to re-investigate immunocytochemically the localization of the enzyme in the brain and spinal cord using specific antibodies against the manganese (Mn-SOD) and copper/zinc (Cu/Zn-SOD) containing isoenzyme in combination with cell type specific markers. CNS tissue sections were analyzed by confocal laser scanning microscopy and digital photo imaging. Cu/Zn-SOD immunoreactivity was found to be located predominantly in astrocytes throughout the CNS. The staining was found in the cytoplasm, in cellular processes and, less intensive, in the nucleus sparing the nucleolus. At a lower level the enzyme was also detectable in neuronal perikarya and in structures of the neuropil. Motoneurons of the spinal cord displayed an enhanced Cu/Zn-SOD staining intensity, when compared to brain neurons. In contrast the Mn-containing isoenzyme was predominantly localized to neurons and their processes throughout the brain and the spinal cord. Confirming the mitochondrial localization of the enzyme, a granular staining pattern sparing the nucleus was observed. Mn-SOD stained mitochondria were also seen in astroglial cells but the staining intensity was, on the whole, much lower compared to neurons, and often hardly detectable. It seems reasonable to conclude that differences in the basal content of SOD-isoenzymes may contribute to different cellular susceptibilities in neurodegenerative processes that are accompanied by oxidative stress.
journal_name
Gliajournal_title
Gliaauthors
Lindenau J,Noack H,Possel H,Asayama K,Wolf Gdoi
10.1002/(sici)1098-1136(20000101)29:1<25::aid-gliasubject
Has Abstractpub_date
2000-01-01 00:00:00pages
25-34issue
1eissn
0894-1491issn
1098-1136pii
10.1002/(SICI)1098-1136(20000101)29:1<25::AID-GLIAjournal_volume
29pub_type
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