Cognitive deficits in mice lacking Nsun5, a cytosine-5 RNA methyltransferase, with impairment of oligodendrocyte precursor cells.

Abstract:

:Williams-Beuren syndrome (WBS) is a microdeletion disorder with cognitive phenotype. NSUN5 gene, which encodes a cytosine-5 RNA methyltransferase, is located in WBS deletion locus. To investigate the influence of NSUN5 deletion on cognitive behaviors, we produced single-gene Nsun5 knockout (Nsun5-KO) mice. Here, we report that adult Nsun5-KO mice showed spatial cognitive deficits. Size of the brain and hippocampal structures and the number of CA1 or CA3 pyramidal cells in Nsun5-KO mice did not differ from WT mice. Basal properties of Schaffer collateral-CA1 synaptic transmission in Nsun5-KO mice were unchanged, but NMDA receptor (NMDAr)-dependent long-term potentiation (LTP) was not induced. The NMDA-evoked current in CA1 pyramidal cells was reduced in Nsun5-KO mice without the changes in expression and phosphorylation of NMDAr subunits NR2A and NR2B. Although the protein level of AMPA receptor subunit GluR2 was attenuated in Nsun5-KO mice, the AMPA-evoked current was not altered. Hippocampal immuno-staining showed the selective expression of Nsun5 in NG2 or PDGFRα labeled oligodendrocyte precursor cells (OPCs), but not in pyramidal cells or astrocytes. Analysis of RT-PCR determined the Nsun5 expression in purified populations of OPCs rather than neurons or astrocytes. The Nsun5 deficiency led to decreases in the number and neurite outgrowth of OPCs in the hippocampal CA1 and DG, with the decline in NG2 expression and OPCs proliferation. These findings indicate that the Nsun5 deletion suppresses NMDAr activity in neuronal cells probably through the disrupted development and function of OPCs, leading to deficits in NMDAr-dependent LTP and spatial cognitive abilities.

journal_name

Glia

journal_title

Glia

authors

Zhang T,Chen P,Li W,Sha S,Wang Y,Yuan Z,Shen B,Chen L

doi

10.1002/glia.23565

subject

Has Abstract

pub_date

2019-04-01 00:00:00

pages

688-702

issue

4

eissn

0894-1491

issn

1098-1136

journal_volume

67

pub_type

杂志文章

相关文献

GLIA文献大全
  • Na+ channels of Müller (glial) cells isolated from retinae of various mammalian species including man.

    abstract::Within the last few years, the expression of voltage-dependent, TTX-sensitive Na+ channels has been demonstrated in several types of neuroglial cells such as astrocytes and Schwann cells. Recently, we reported the occurrence of such Na+ currents in retinal Müller (glial) cells from dog and cat. This paper deals with t...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440100304

    authors: Chao TI,Skachkov SN,Eberhardt W,Reichenbach A

    更新日期:1994-03-01 00:00:00

  • Chronically denervated rat Schwann cells respond to GGF in vitro.

    abstract::C-erbB receptor/neuregulin signalling plays a significant role in Schwann cell function. In vivo, Schwann cells up-regulate expression of c-erbB receptors in the first month after injury, but receptor expression is down-regulated with time to levels that are not detectable immunohistochemically. The inability of chron...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Li H,Wigley C,Hall SM

    更新日期:1998-11-01 00:00:00

  • AT1 receptor inhibition prevents astrocyte degeneration and restores vascular growth in oxygen-induced retinopathy.

    abstract::We investigated the effect of receptor blockade induced by an angiotensin II type-1 receptor antagonist (AT(1)-RB) on glial and vascular changes in oxygen-induced retinopathy (OIR), a model of retinopathy of prematurity (ROP). OIR was induced in Sprague-Dawley rats by exposure to 80% oxygen from postnatal (P) days 0-1...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20680

    authors: Downie LE,Pianta MJ,Vingrys AJ,Wilkinson-Berka JL,Fletcher EL

    更新日期:2008-08-01 00:00:00

  • Microglia are less pro-inflammatory than myeloid infiltrates in the hippocampus of mice exposed to status epilepticus.

    abstract::Activated microglia, astrogliosis, expression of pro-inflammatory cytokines, blood brain barrier (BBB) leakage and peripheral immune cell infiltration are features of mesial temporal lobe epilepsy. Numerous studies correlated the expression of pro-inflammatory cytokines with the activated morphology of microglia, attr...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.23008

    authors: Vinet J,Vainchtein ID,Spano C,Giordano C,Bordini D,Curia G,Dominici M,Boddeke HW,Eggen BJ,Biagini G

    更新日期:2016-08-01 00:00:00

  • Ultrastructural study of phagocytic activities of young astrocytes in injured neonatal rat brain following intracerebral injection of colloidal carbon.

    abstract::The cellular reaction to injury in the mature central nervous system (CNS) has been extensively studied in both man and animals, while a detailed study of the reaction of the immature CNS to injury is lacking in the literature. This study was undertaken to elucidate the response of young astrocytes following injection...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440010306

    authors: Al-Ali SY,Al-Zuhair AG,Dawod B

    更新日期:1988-01-01 00:00:00

  • Meteorin regulates angiogenesis at the gliovascular interface.

    abstract::Brain microvasculature requires a coordinated interaction between endothelial cells and astrocytes at the gliovascular interface. However, the role of the factors involved in that interaction and expressed by these cells is poorly understood. In this study, we demonstrate that Meteorin is highly expressed in astrocyte...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20600

    authors: Park JA,Lee HS,Ko KJ,Park SY,Kim JH,Choe G,Kweon HS,Song HS,Ahn JC,Yu YS,Kim KW

    更新日期:2008-02-01 00:00:00

  • Downregulation of miR-7116-5p in microglia by MPP+ sensitizes TNF-α production to induce dopaminergic neuron damage.

    abstract::Activation of microglia resulting in exacerbated inflammation expression plays an important role in degeneration of dopaminergic (DA) neurons in the pathogenesis of Parkinson's disease (PD). However, how this enhanced inflammation is induced in microglia remains largely unclear. Here, in the mouse PD model induced by ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.23153

    authors: He Q,Wang Q,Yuan C,Wang Y

    更新日期:2017-08-01 00:00:00

  • Alterations in metabolism and gap junction expression may determine the role of astrocytes as "good samaritans" or executioners.

    abstract::Our knowledge of astroglia and their physiological and pathophysiological role(s) in the central nervous system (CNS) has grown during the past decade, revealing a complex picture. It is becoming increasingly clear that glia play a significant role in the homeostasis and function of the CNS and that neurons should no ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20213

    authors: Farahani R,Pina-Benabou MH,Kyrozis A,Siddiq A,Barradas PC,Chiu FC,Cavalcante LA,Lai JC,Stanton PK,Rozental R

    更新日期:2005-06-01 00:00:00

  • Physiology of transformed glial cells.

    abstract::Much of our present knowledge of glial cell function stems from studies of glioma cell lines, both rodent (C6, C6 polyploid, and TR33B) and human (1321N1, 138MG, D384, R-111, T67, Tp-276MG, Tp-301MG, Tp-483MG, Tp-387MG, U-118MG, U-251MG, U-373MG, U-787MG, U-1242MG, and UC-11MG). New methods such as patch clamp and Ca2...

    journal_title:Glia

    pub_type: 杂志文章,评审

    doi:10.1002/glia.440150305

    authors: Brismar T

    更新日期:1995-11-01 00:00:00

  • Long-chain ceramide is elevated in presenilin 1 (PS1M146V) mouse brain and induces apoptosis in PS1 astrocytes.

    abstract::The pro-apoptotic sphingolipid ceramide plays an emergent role in the etiology of Alzheimer's disease (AD), although its function for neurodegeneration is not known. We determined the concentration and composition of ceramide in hippocampal tissue from newborn presenilin 1 (PS1) knock-in (PS1M146V) mice, a mouse model...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20626

    authors: Wang G,Silva J,Dasgupta S,Bieberich E

    更新日期:2008-03-01 00:00:00

  • Capacitative Ca2+ influx in glial cells is inhibited by glycolytic inhibitors.

    abstract::In non-excitable cells, stimulation of phosphoinositide (PI) turnover and inhibition of the endoplasmic reticulum (ER) Ca2+-ATPase are methods commonly used to deplete calcium stores, resulting in a capacitative Ca2+ influx (i.e., Ca2+ release-activated Ca2+ influx). Since this Ca2+ influx in glial cells has not been ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-1136(199711)21:3<315::aid-glia6

    authors: Wu ML,Kao EF,Liu IH,Wang BS,Lin-Shiau SY

    更新日期:1997-11-01 00:00:00

  • Effect of elevated K(+), hypotonic stress, and cortical spreading depression on astrocyte swelling in GFAP-deficient mice.

    abstract::Glial fibrillary acidic protein (GFAP) is the main component of intermediate filaments in astrocytes. To assess its function in astrocyte swelling, we compared astrocyte membrane properties and swelling in spinal cord slices of 8- to 10-day-old wild-type control (GFAP(+/+)) and GFAP-knockout (GFAP(-/-)) mice. Membrane...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.1084

    authors: Anderová M,Kubinová S,Mazel T,Chvátal A,Eliasson C,Pekny M,Syková E

    更新日期:2001-09-01 00:00:00

  • Non-myelin-forming perisynaptic schwann cells express protein zero and myelin-associated glycoprotein.

    abstract::Perisynaptic Schwann cells (PSCs) envelop axonal terminals and are physiologically distinct from the nearby myelinating Schwann cells (MSCs), which surround the same innervating motor axons. PSCs have special functions at the neuromuscular synapse, where they detect and can modulate neurotransmitter release. Although ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-1136(199908)27:2<101::aid-glia1

    authors: Georgiou J,Charlton MP

    更新日期:1999-08-01 00:00:00

  • Astrocyte-derived metallothionein protects dopaminergic neurons from dopamine quinone toxicity.

    abstract::Our previous studies demonstrated the involvement of quinone formation in dopaminergic neuron dysfunction in the L-DOPA-treated parkinsonian model and in methamphetamine (METH) neurotoxicity. We further reported that the cysteine-rich metal-binding metallothionein (MT) family of proteins protects dopaminergic neurons ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.21112

    authors: Miyazaki I,Asanuma M,Kikkawa Y,Takeshima M,Murakami S,Miyoshi K,Sogawa N,Kita T

    更新日期:2011-03-01 00:00:00

  • Recovery from lactacidosis-induced glial cell swelling with the aid of exogenous anion channels.

    abstract::Hypotonic challenge induces transient swelling in glial cells, which is typically followed by a regulatory volume decrease (RVD). In contrast, lactic acidosis (lactacidosis) induces persistent cell swelling in astrocytes without an accompanying RVD. In the present study, we studied the mechanisms by which lactacidosis...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.10190

    authors: Nabekura T,Morishima S,Cover TL,Mori S,Kannan H,Komune S,Okada Y

    更新日期:2003-02-01 00:00:00

  • Cell therapy for spinal cord injury with olfactory ensheathing glia cells (OECs).

    abstract::The prospects of achieving regeneration in the central nervous system (CNS) have changed, as most recent findings indicate that several species, including humans, can produce neurons in adulthood. Studies targeting this property may be considered as potential therapeutic strategies to respond to injury or the effects ...

    journal_title:Glia

    pub_type: 杂志文章,评审

    doi:10.1002/glia.23282

    authors: Gómez RM,Sánchez MY,Portela-Lomba M,Ghotme K,Barreto GE,Sierra J,Moreno-Flores MT

    更新日期:2018-07-01 00:00:00

  • T- and B-cell responses to myelin oligodendrocyte glycoprotein in experimental autoimmune encephalomyelitis and multiple sclerosis.

    abstract::The identification of myelin oligodendrocyte glycoprotein (MOG) as a target for autoantibody-mediated demyelination in experimental autoimmune encephalomyelitis (EAE) resulted in the re-evaluation of the role of B cell responses to myelin autoantigens in the immunopathogenesis of multiple sclerosis. MOG is a central n...

    journal_title:Glia

    pub_type: 杂志文章,评审

    doi:10.1002/glia.1111

    authors: Iglesias A,Bauer J,Litzenburger T,Schubart A,Linington C

    更新日期:2001-11-01 00:00:00

  • Neuronal hyperactivity induces astrocytic expression of neurocan in the adult rat hippocampus.

    abstract::Extracellular matrix molecules are involved in the cellular functions of proliferation, migration, morphological differentiation, and synaptic plasticity. One candidate molecule of the extracellular matrix is the chondroitin sulfate proteoglycan neurocan. To determine whether neurocan expression is regulated by neuron...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20329

    authors: Schwarzacher SW,Vuksic M,Haas CA,Burbach GJ,Sloviter RS,Deller T

    更新日期:2006-05-01 00:00:00

  • The identification of transcriptional targets of Ascl1 in oligodendrocyte development.

    abstract::The basic helix-loop-helix (bHLH) transcription factor Ascl1 plays crucial roles in both oligodendrocyte development and neuronal development; however, the molecular target of Ascl1 in oligodendrocyte progenitor cells (OPCs) remains elusive. To identify the downstream targets of Ascl1 in OPCs, we performed gene expres...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.22369

    authors: Ueno T,Ito J,Hoshikawa S,Ohori Y,Fujiwara S,Yamamoto S,Ohtsuka T,Kageyama R,Akai M,Nakamura K,Ogata T

    更新日期:2012-10-01 00:00:00

  • Interactions between TLR7 and TLR9 agonists and receptors regulate innate immune responses by astrocytes and microglia.

    abstract::Toll-like receptors 7 (TLR7) and 9 (TLR9) are important mediators of innate immune responses. Both receptors are located in endosomal compartments, recognize nucleic acids, and signal via Myeloid differentiation factor 88 (MyD88). In the current study, we analyzed TLR7 and TLR9 induced activation of astrocytes and mic...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20952

    authors: Butchi NB,Du M,Peterson KE

    更新日期:2010-04-15 00:00:00

  • Pure astrocyte cultures derived from cells isolated from mature brain.

    abstract::Enriched preparations of oligodendrocytes, isolated either from adult bovine brain or from 30-day-old rat brain, eventually yield cultures in MEM-15% calf serum that contain, in addition to oligodendrocytes, proliferating astrocytes and variable numbers of fibroblast-like cells. If these cultures are switched to a ser...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440010608

    authors: Norton WT,Farooq M,Chiu FC,Bottenstein JE

    更新日期:1988-01-01 00:00:00

  • Potential role of radial glia in adult neurogenesis of teleost fish.

    abstract::Persistence of radial glia within the adult central nervous system is a widespread phenomenon among fish. Based on a series of studies in the teleost species Apteronotus leptorhynchus, we propose that one function of this persistence is the involvement of radial glia in adult neurogenesis, i.e., the generation and fur...

    journal_title:Glia

    pub_type: 杂志文章,评审

    doi:10.1002/glia.10236

    authors: Zupanc GK,Clint SC

    更新日期:2003-07-01 00:00:00

  • Astrocytes protect neurons from neurotoxic injury by serum glutamate.

    abstract::Serum is used widely for culturing neurons and glial cells, and is thought to provide essential, albeit undefined, factors such as hormones, growth factors, and trace elements that promote the growth of cells in vitro. Moreover, serum can have profound effects on cell proliferation, differentiation, and cell morpholog...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-1136(199803)22:3<237::aid-glia3

    authors: Ye ZC,Sontheimer H

    更新日期:1998-03-01 00:00:00

  • Altered immune response to CNS viral infection in mice with a conditional knock-down of macrophage-lineage cells.

    abstract::Neuroadapted Sindbis Virus (NSV) is a neuronotropic virus that causes hindlimb paralysis in susceptible mice and rats. The authors and others have demonstrated that though death of infected motor neurons occurs, bystander death of uninfected neurons also occurs and both contribute to the paralysis that ensues followin...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20359

    authors: Carmen J,Gowing G,Julien JP,Kerr D

    更新日期:2006-08-01 00:00:00

  • Expression of distinct splice variants of the stem cell marker prominin-1 (CD133) in glial cells.

    abstract::Prominin-1 (CD133) is a cholesterol-interacting pentaspan membrane glycoprotein specifically associated with plasma membrane protrusions. Prominin-1 is expressed by various stem and progenitor cells, notably neuroepithelial progenitors found in the developing embryonic brain. Here, we further investigated its expressi...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20812

    authors: Corbeil D,Joester A,Fargeas CA,Jászai J,Garwood J,Hellwig A,Werner HB,Huttner WB

    更新日期:2009-06-01 00:00:00

  • Activation of interferon signaling pathways in spinal cord astrocytes from an ALS mouse model.

    abstract::Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder affecting predominantly motor neurons. Recent studies suggest that the disease progression of ALS is non-cell-autonomous, although the interaction between neurons and glial cells in different disease stages is not entirely clear. Here, we ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.21167

    authors: Wang R,Yang B,Zhang D

    更新日期:2011-06-01 00:00:00

  • Response of rat cerebral cortical astrocytes to freeze- or cobalt-induced injury: an immunocytochemical and gap-FRAP study.

    abstract::Astrocytic response in the immediate vicinity of freeze- and cobalt-induced lesions has been examined at the light and ultrastructural level. However, the temporal and spatial distribution of astrocytic reactivity throughout the rat cerebral cortex, using glial fibrillary acidic protein (GFAP) immunolabeling, has not ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440030606

    authors: Anders JJ,Niedermair S,Ellis E,Salopek M

    更新日期:1990-01-01 00:00:00

  • Proinflammatory cytokine regulation of cyclic AMP-phosphodiesterase 4 signaling in microglia in vitro and following CNS injury.

    abstract::Cyclic AMP suppresses immune cell activation and inflammation. The positive feedback loop of proinflammatory cytokine production and immune activation implies that cytokines may not only be regulated by cyclic AMP but also conversely regulate cyclic AMP. This study examined the effects of tumor necrosis factor (TNF)-α...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.22401

    authors: Ghosh M,Garcia-Castillo D,Aguirre V,Golshani R,Atkins CM,Bramlett HM,Dietrich WD,Pearse DD

    更新日期:2012-12-01 00:00:00

  • H-ferritin is the major source of iron for oligodendrocytes.

    abstract::There is a critical relationship between oligodendrocyte development, myelin production, and iron bioavailability. Iron deficiency leads to hypomyelination both in humans and animal models, and the neurological sequelae of hypomyelination are significant. Therefore, understanding molecular mechanisms of iron import in...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.21164

    authors: Todorich B,Zhang X,Connor JR

    更新日期:2011-06-01 00:00:00

  • Media that support the growth and differentiation of oligodendrocytes do not induce olfactory ensheathing cells to express a myelinating phenotype.

    abstract::The glial cells that ensheath olfactory axons are referred to as ensheathing cells. In vivo, these non-myelinating glial cells express a mixture of astrocyte-specific and Schwann cell-specific phenotypic features with the former cellular phenotype predominating, but in vitro can assemble a myelin sheath when co-cultur...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440100408

    authors: Doucette R,Devon R

    更新日期:1994-04-01 00:00:00