Abstract：
:Genetic immunization holds promise as a vaccination method, but has so far proven ineffective in large primate and human trials. Herein, we examined the relative merits of genetic immunization and peptide immunization using bacteriophage λ. Bacteriophage λ has proven effective in immune challenge models using both immunization methods, but there has never been a direct comparison of efficacy and of the quality of immune response. In the current study, this vector was produced using a combination of cis and trans phage display. When antibody titers were measured from immunized animals together with IL-2, IL-4 and IFNγ production from splenocytes in vitro, we found that proteins displayed on λ were superior at eliciting an immune response in comparison to genetic immunization with λ. We also found that the antibodies produced in response to immunization with λ displayed proteins bound more epitopes than those produced in response to genetic immunization. Finally, the general immune response to λ inoculation, whether peptide or genetic, was dominated by a Th1 response, as determined by IFNγ and IL-4 concentration, or by a higher concentration of IgG2a antibodies.
journal_name
Vaccinejournal_title
Vaccineauthors
Thomas BS,Nishikawa S,Ito K,Chopra P,Sharma N,Evans DH,Tyrrell DL,Bathe OF,Rancourt DEdoi
10.1016/j.vaccine.2011.12.070subject
Has Abstractpub_date
2012-02-01 00:00:00pages
998-1008issue
6eissn
0264-410Xissn
1873-2518pii
S0264-410X(11)02010-Xjournal_volume
30pub_type
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