Abstract：
:For the production of a chemically inactivated Parapoxvirus ovis (PPVO), an adherent bovine kidney cell line was cultivated on Cytodex-3 microcarriers in suspension culture. The inactivated and purified virus particles have shown immune modulatory activity in several animal models. PPVO was produced by a biphasic batch process at the 3.5 and 10 L scale. Aeration was realised by bubble-free membrane oxygenation via a tube stator with a central two-blade anchor impeller. In order to increase efficiency, process robustness and safety, the established process was optimised. The cell line was adapted to a protein-free medium (except recombinant insulin) in order to increase biosafety. A scale up to a 50 L pilot plant with direct cell expansion was performed successfully. In parallel, the biphasic batch process was optimised with special emphasis on different operating conditions (cell number, Multiplicity of Infection (MOI), etc.) and process management (fed-batch, dialysis, etc.). The quality and concentration of the purified virus particles was assessed by quantitative electron microscopy, residual host cell protein and DNA-content and, finally, biologic activity in a transgenic mouse model. This integrated approach led to a new, safe, robust and highly productive large-scale production process, called "Volume-Expanded-Fed" Batch with cell densities up to 6-7e06 cells/mL. By subsequent dilution of infected cells into the next process scale, an increase in total productivity by a factor of 40 (related to an established biphasic batch process) was achieved.
journal_name
Vaccinejournal_title
Vaccineauthors
Pohlscheidt M,Langer U,Minuth T,Bödeker B,Apeler H,Hörlein HD,Paulsen D,Rübsamen-Waigmann H,Henzler HJ,Reichl Udoi
10.1016/j.vaccine.2008.01.032subject
Has Abstractpub_date
2008-03-17 00:00:00pages
1552-65issue
12eissn
0264-410Xissn
1873-2518pii
S0264-410X(08)00040-6journal_volume
26pub_type
杂志文章相关文献
VACCINE文献大全abstract：:Little is known of men's knowledge of cervical cancer and its links with human papillomavirus (HPV), or of their attitudes and beliefs about HPV vaccination. This is despite men's sexual behaviour contributing to HPV transmission and their potential role in deciding whether their children are vaccinated against HPV. T...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2009.02.101
更新日期：2009-05-14 00:00:00
abstract：:Using a fixed dose of antigen, the immune response to detoxified mutants of LT-WT following intranasal (i.n.), subcutaneous (s.c.) and oral (i.g.) immunisation has been studied. When given i.n., both LT-WT and mutant toxin, K63, generated significant levels of toxin-specific IgG in the serum, and the levels of IgA in ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(98)80100-x
更新日期：1998-07-01 00:00:00
abstract：:Human respiratory syncytial virus (RSV) is a leading cause of severe respiratory disease and hospitalizations in infants and young children. It also causes significant morbidity and mortality in elderly and immune compromised individuals. No licensed vaccine currently exists. Parainfluenza virus 5 (PIV5) is a paramyxo...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2014.03.049
更新日期：2014-05-23 00:00:00
abstract：:The bioavailability of oseltamivir phosphate and oseltamivir carboxylate were assessed in healthy volunteers when delivered as a solution of the active pharmaceutical ingredient (API) compared with the commercial capsule formulation. The 90% confidence intervals (CIs) for the ratios of the two treatments (capsule vers...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2006.05.080
更新日期：2006-11-10 00:00:00
abstract：:Ten prospective clinical trials conducted in elderly and high-risk adults have failed to show that pneumococcal vaccine prevents pneumococcal bacteraemia and all pneumonia. Several of these trials focused on unrepresentative populations and most had serious methodological problems. Few adequately considered sample siz...
journal_title：Vaccine
pub_type： 杂志文章,meta分析
doi：10.1016/j.vaccine.2003.09.027
更新日期：2004-02-25 00:00:00
abstract：:Promotional campaigns recommend immunisation against influenza in healthcare workers (HCWs) but the uptake in this group remains low. We conducted a survey study during the 2008-2009 influenza vaccination period amongst future HCWs to quantify uptake and identify barriers to immunisation. Overall uptake was 8.0% (95% ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2010.04.066
更新日期：2010-06-23 00:00:00
abstract：BACKGROUND:FP-01.1 is a novel synthetic influenza A vaccine consisting of six fluorocarbon-modified 35-mer peptides that encapsulate multiple CD4+ and CD8+ T-cell epitopes and is designed to induce an immune response across a broad population. METHODS:FP-01.1 was evaluated for safety and immunogenicity in a randomised...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2014.06.006
更新日期：2015-01-03 00:00:00
abstract：BACKGROUND:The 2015/2016 influenza season was characterized in Europe by the circulation of A(H1N1)pdm09 clade 6B.1 and B/Victoria-lineage influenza viruses. The components of the vaccines used in the current and past two seasons in the Valencia region were similar but not well matched to the 2015/2016 dominant influen...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2017.10.100
更新日期：2017-12-19 00:00:00
abstract：:In the United States, co-administration of tetanus toxoid, reduced diphtheria toxoid and acellular pertussis (Tdap) vaccine and tetravalent meningococcal conjugate vaccine (MCV4) is recommended in adolescents. In this clinical study, 1341 adolescents received Tdap (Boostrix® GlaxoSmithKline) and MCV4 (Menactra®, Sanof...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2010.11.057
更新日期：2011-01-29 00:00:00
abstract：:Among strategies aimed at developing new nanoparticle-based vaccines, exosomes hold much promise. They are nanovesicles released by basically all eukaryotic cell types originating from intraluminal vesicles which accumulate in multivesicular bodies. Exosomes have immunogenic properties whose strength correlates with t...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2012.10.010
更新日期：2012-11-26 00:00:00
abstract：:Pneumococcal conjugate vaccines (PCV) may reduce nasopharyngeal carriage (NPC) of Streptococcus pneumoniae vaccine strains (VT), but serotype replacement with non-vaccine strains (NVT) has been reported. Bacterial NPC after PHiD-CV vaccination was assessed in the second year of life. Open descriptive study of NPC repo...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2010.12.086
更新日期：2011-02-24 00:00:00
abstract：:We investigated several methods to immunize the abomasum (fourth and gastric stomach) of kid goats by direct (abomasal) or distal (rectal or nasal) routes utilizing mucosal antigens isolated from the abomasal parasite, Haemonchus contortus. Direct (ultrasound guided), immunization of the abomasal mucosa together with ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2011.02.003
更新日期：2011-04-05 00:00:00
abstract：:Hepatitis B surface antigen (HBsAg) was detected in 3.3% of 7162 pregnant Tunisian women tested and HBeAg in 9.6% of the HBsAg-positive mothers. Family members of 46 of these HBsAg-positive mothers (33 husbands and 61 children aged 1-6 years) were investigated for the presence of HBV markers. HBsAg was detected in 21%...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(94)90205-4
更新日期：1994-01-01 00:00:00
abstract：:Hepatitis B infections are responsible for more than 300 thousand deaths per year in the Western Pacific Region. Because of this high burden, the countries and areas of the Region established a goal of reducing hepatitis B chronic infection prevalence among children to less than 1% by 2017. This study was conducted to...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2016.03.060
更新日期：2016-05-27 00:00:00
abstract：:In order to evaluate the immunogenicity, safety, and tolerability of the MF-59 adjuvanted seasonal influenza vaccine in children and adolescents with juvenile idiopathic arthritis (JIA) treated with different anti-rheumatic drugs, 60 pediatric patients with JIA (30 treated with disease-modifying anti-rheumatic drugs [...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2011.11.083
更新日期：2012-01-20 00:00:00
abstract：:DNA vaccines have considerable potential for the prophylaxis and therapy of a range of diseases, but their potential has not been realised largely due to poor immunogenicity. Fas ligand is a pro-apoptotic molecule, able to induce death of Fas expressing cells. We describe the construction of a DNA vaccine encoding a c...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2006.11.059
更新日期：2007-03-08 00:00:00
abstract：:Yellow fever is an acute infectious disease caused by prototype virus of the genus Flavivirus. It is endemic in Africa and South America where it represents a serious public health problem causing epidemics of hemorrhagic fever with mortality rates ranging from 20% to 50%. There is no available antiviral therapy and v...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2015.03.077
更新日期：2015-08-20 00:00:00
abstract：:The Global Meningococcal Initiative (GMI) is composed of an international group of scientists, clinicians and public health officials with expertise in meningococcal immunology, epidemiology and prevention. The primary goal of the GMI is the promotion of the global prevention of invasive meningococcal disease through ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2011.02.058
更新日期：2011-04-18 00:00:00
abstract：:In this study, the protection afforded against aerosolized Yersinia pestis by injection of an alhydrogel-adsorbed sub-unit vaccine has been compared with that given by an existing killed whole cell vaccine licensed for human use. The sub-unit vaccine protected mice against exposure to > 10(4) colony-forming units (c.f...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(96)00303-9
更新日期：1997-07-01 00:00:00
abstract：:New and improved vaccines and delivery systems are increasingly being developed for prevention, treatment and diagnosis of human diseases. Prior to their use in humans, all new biological products must undergo pre-clinical evaluation. These pre-clinical studies are important not only to establish the biological proper...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2005.01.135
更新日期：2006-04-12 00:00:00
abstract：:We investigated the therapeutic effects of romurtide, a synthetic muramyl dipeptide derivative, on experimental thrombocytopenia induced by carboplatin in cynomolgus monkeys. A prolonged thrombocytopenia due to a severe myelosuppression was induced by carboplatin. Romurtide given subcutaneously elevated significantly ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(96)00064-3
更新日期：1996-10-01 00:00:00
abstract：:Among the numerous targets which can be used for the development of vaccines against Pseudomonas aeruginosa we focused on the outer membrane proteins OprF and OprI. The C-terminal part of OprF from aa 190 to aa 350 was investigated for its conservation and its localization of B-cell epitopes. A hybrid protein which co...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(98)00420-4
更新日期：1999-03-26 00:00:00
abstract：:Most traditional vaccines are administered via the intramuscular route. Other routes of administration however, can induce equal or improved protective memory responses and might provide practical advantages such as needle-free immunization, dose sparing and induction of tissue-specific (mucosal) immunity. Here we exp...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2018.01.077
更新日期：2018-03-07 00:00:00
abstract：:Four different preparations of hepatitis B antigen (HBsAg) were tested in parallel with respect to their ability to elicit anti-HBs in guinea-pigs. We compared the effect of low doses (5 micrograms single dose and 30 micrograms total amount) of human plasma derived and recombinant HBsAg, in purified forms or prepared ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(90)90014-d
更新日期：1990-12-01 00:00:00
abstract：:In a previous work we selected montanide ISA720 (M-ISA720) among different adjuvants for the vaccination with a V3 loop based multi-epitope polypeptide TAB9. In this paper we present the evaluation of the toxicity and immunogenicity of this formulation in non-human primates. TAB9 in M-ISA720 was highly immunogenic in ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(98)00358-2
更新日期：1999-05-04 00:00:00
abstract：:Influenza A virus causes respiratory tract infections, which are occasionally complicated by secondary bacterial infections. Influenza A virus replicates in epithelial cells and leukocytes resulting in the production of chemokines and cytokines, which favor the extravasation of blood mononuclear cells and the developm...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/s0264-410x(00)00275-9
更新日期：2000-12-08 00:00:00
abstract：BACKGROUND:Seasonal influenza infections among young children in China lead to substantial numbers of hospitalizations and financial burden. This study assessed the seasonal influenza vaccine effectiveness (VE) against laboratory confirmed medically attended influenza illness among children in Suzhou, China, from Octob...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2016.03.056
更新日期：2016-05-05 00:00:00
abstract：:Japanese encephalitis (JE) vaccine has been used in childhood immunization programmes in Asia for many years. Also travellers from other parts of the world have been vaccinated before travelling in Asian countries. Recommendations for the use of JE vaccine are still debated because severe cases of adverse reactions ha...
journal_title：Vaccine
pub_type： 临床试验,杂志文章
doi：10.1016/s0264-410x(97)00020-0
更新日期：1997-08-01 00:00:00
abstract：BACKGROUND:World Health Organization has recommended the introduction of pneumococcal conjugate vaccine (PCV) in the childhood immunisation programme of all the countries in the world. In lieu of its introduction in India, there is a need to generate evidence on cost-effectiveness of this vaccine. The current study loo...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2018.12.004
更新日期：2019-01-21 00:00:00
abstract：:Genetic diversity and hypermutation contribute to difficulties in developing a vaccine against viruses like HIV and influenza. There are currently no known immune correlates of protection against HIV. This has made the development of a vaccine against HIV that would provide sterilizing immunity in the near future an i...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2007.12.008
更新日期：2008-06-06 00:00:00
