Abstract：
:Recently, Bacillus subtilis spores were shown to be endowed with strong adjuvant capacity when co-administered with purified antigenic proteins. In the present study we assessed whether spores possess adjuvant properties when combined with DNA vaccines. We showed that B. subtilis spores promoted the activation of dendritic cells in vitro and induced migration of pro-inflammatory cells after parenteral administration to mice. Likewise, co-administration of spores with a DNA vaccine encoding the human papillomavirus type 16 (HPV-16) E7 protein enhanced the activation of antigen-specific CD8(+) T cell responses in vivo. Mice immunized with the DNA vaccine admixed with spores presented a protective immunity increase to previously implanted tumor cells, capable of expressing HPV-16 oncoproteins. Finally, we observed that the adjuvant effect can vary accordingly to the number of co-administered spores which may be ascribed with the ability to induce. Collectively, the present results demonstrate for the first time that B. subtilis spores can also confer adjuvant effects to DNA vaccines.
journal_name
Vaccinejournal_title
Vaccineauthors
Aps LR,Diniz MO,Porchia BF,Sales NS,Moreno AC,Ferreira LCdoi
10.1016/j.vaccine.2015.03.043subject
Has Abstractpub_date
2015-05-11 00:00:00pages
2328-34issue
20eissn
0264-410Xissn
1873-2518pii
S0264-410X(15)00351-5journal_volume
33pub_type
杂志文章相关文献
VACCINE文献大全abstract：:There is accumulating evidence that many invertebrates including insects can acquire enhanced immune protection against subsequently pathogens infection through immune priming. However, whether the toxin protein from pathogenic bacteria can induce such priming response remains unclear. Here we cloned, expressed and pu...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2015.09.046
更新日期：2015-11-17 00:00:00
abstract：:Long-term effects of therapeutic vaccination of human immunodeficiency virus (HIV)-1-infected subjects with HIV-1 p17/p24:Ty virus-like particles (p24-VLP) on progression to AIDS, death, a CD4 cell count journal_title：Vaccine pub_type： 临床试验,杂志文章,多中心研究 doi：10.1016/s0264-410x(02)00102-0 更新日期：2002-05-22 00:00:00
abstract：:Vaccination during pregnancy is increasingly being used as an effective approach for protecting both young infants and their mothers from serious infections. Drawing conclusions from published studies in this area can be difficult because of the inability to compare vaccine trial results across different studies and s...
journal_title：Vaccine
pub_type： 指南,杂志文章
doi：10.1016/j.vaccine.2016.07.032
更新日期：2016-12-01 00:00:00
abstract：:Hepatitis B virus (HBV) infection and HBV-associated diseases are prevalent in many parts of the Middle East and North Africa. Postnatal horizontal childhood transmission appears to be the predominant method by which high hepatitis B carrier rates are maintained. To control hepatitis B in this region, mass immunizatio...
journal_title：Vaccine
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
abstract：:We have previously shown that vaccination of BALB/c mice with a combination of BCG plus killed Leishmania promastigotes, applied by the i.p. route 10 and 3 days before Trypanosoma cruzi inoculation, prolonged their survival and decreased their parasitaemia. In the present study we show that the BCG-Leishmania vaccine ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(99)00426-0
更新日期：2000-03-06 00:00:00
abstract：BACKGROUND:Rabies is a neglected zoonotic disease with a global burden of approximately 59,000 human deaths a year. Once clinical symptoms appear, rabies is almost invariably fatal; however, with timely and appropriate post-exposure prophylaxis (PEP) consisting of wound washing, vaccine, and in some cases rabies immuno...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2019.04.024
更新日期：2019-10-03 00:00:00
abstract：:Dengue virus (DENV) is the causal agent of severe disease and, in some cases, mortality in humans, but no licensed vaccines against dengue are available. An effective vaccine against dengue requires long-term humoral and cellular immune responses. Several viral proteins have been the subjects of intense research, espe...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2013.03.009
更新日期：2013-05-01 00:00:00
abstract：:We described here the characterization of Schistosoma mansoni egg antigens recognized by human monoclonal antibodies B10 (HmAb-B10) and D5 (HmAb-D5). SDS-PAGE and Western blot analysis revealed that these monoclonals recognized two antigens of M W 44.7/56.8 kDa, with pI of 7.0 and 7.8, respectively. The passive transf...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(96)00305-2
更新日期：1997-06-01 00:00:00
abstract：:A Phase I interventional Clinical Trial was performed with a potential tuberculosis vaccine, based on detoxified cellular fragments of M. tuberculosis, named RUTI. The objective was to evaluate the safety profile and T-cell immune responses over a 6-month period following subcutaneous inoculation. The double-blind, ra...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2009.09.134
更新日期：2010-01-22 00:00:00
abstract：:In the face of an almost unprecedented threat of a global pandemic of influenza it is imperative that stockpiling of appropriate drugs and devices begin now. One vital device is an appropriate syringe for delivering vaccine. With the potential for millions to be infected and the vaccine supply severely stretched it is...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2006.02.056
更新日期：2006-05-29 00:00:00
abstract：:Malignant catarrhal fever (MCF) is a fatal lymphoproliferative disease of cattle that, in East Africa, results from transmission of the causative virus, alcelaphine herpesvirus 1 (AlHV-1), from wildebeest. A vaccine field trial involving an attenuated AlHV-1 virus vaccine was performed over two wildebeest calving seas...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2015.12.009
更新日期：2016-02-03 00:00:00
abstract：:In the present study, we investigated the duration of protection afforded to pigs immunized in two different prime-boost regimens: one is homologus priming and boosting with a protein vaccine, and the other is priming with a DNA vaccine and boosting with the protein vaccine. Groups of pigs that received the same vacci...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2004.07.012
更新日期：2004-09-28 00:00:00
abstract：:Current available influenza vaccines are safe and effective in preventing influenza. Nevertheless, there is a need for influenza vaccines with improved efficacy in the elderly. This need is underscored by both the observation that influenza has a major clinical and economic impact in the elderly and the fact that curr...
journal_title：Vaccine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.vaccine.2005.04.020
更新日期：2005-07-08 00:00:00
abstract：:Since the production of influenza vaccines is complicated by the continuous variation of these viruses, it would be desirable to develop vaccines that induce cross-protective immunity against influenza virus strains that circulate in subsequent winter epidemics. We have recently demonstrated that antibodies induced af...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(01)00262-6
更新日期：2001-10-12 00:00:00
abstract：:Six peptides, A, B1, B, C, D and E derived from the primary sequence of Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase (SG3PDH) were selected based on lowest homology to human G3PDH and used for immunization of BALB/c mice. Peptides B1 and D induced immunoglobulin (Ig) G1, IgG2a and IgG2b antibodies that...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(03)00180-4
更新日期：2003-07-04 00:00:00
abstract：:To evaluate the utility of plasmid DNA vaccination against disease caused by herpes simplex virus (HSV), we compared the strength of protection against lethal challenge following natural virus infection with that following vaccination with a plasmid encoding HSV glycoprotein gD (gD-DNA). We further determined the cell...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(00)00380-7
更新日期：2001-01-08 00:00:00
abstract：:In clinical trials recombinant-modified vaccinia virus Ankara expressing the Mycobacterium tuberculosis antigen 85A (MVA85A) induces approximately 10 times more effector T cells than any other recombinant MVA vaccine. We have found that in BCG primed subjects MVA85A vaccination reduces transforming growth factor beta ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2008.07.040
更新日期：2008-09-26 00:00:00
abstract：:To evaluate long-term immunogenicity of a virosomal subunit inactivated influenza vaccine in children with asthma, a prospective study was conducted during 2005-2006 influenza season in six public pediatric clinics in Milan and surroundings, Northern Italy. A single dose (0.5 ml) of a virosomal subunit inactivated inf...
journal_title：Vaccine
pub_type： 杂志文章,多中心研究
doi：10.1016/j.vaccine.2007.07.013
更新日期：2007-09-17 00:00:00
abstract：:The purpose of the present research work is to explore the potential of dendrosomes in genetic immunization against hepatitis B. Plasmid DNA encoding pRc/CMV-HBs[S] (5.6 kb), encoding the small region of the hepatitis B surface antigen, was complexed with 5th generation poly(propyleneimine) dendrimer (PPI) in differen...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2008.04.058
更新日期：2008-06-25 00:00:00
abstract：:We demonstrated earlier that immunization with recombinant Leptospira immunoglobulin like protein A (LigA) induced significant protection against virulent Leptospira interrogans serovar Pomona challenge in hamsters. However, the protective immune mechanism remains unclear. In the present study we demonstrated the prot...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.10.029
更新日期：2008-01-10 00:00:00
abstract：:Haemophilus parasuis (H. parasuis) is a swine pathogen responsible for the Glässer's disease, which has received more attention in the past decade due to the increasing economic losses in the pig industry worldwide. As traditional inactive vaccine of H. parasuis has obvious disadvantage, to identify efficient immunopr...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2015.02.023
更新日期：2015-03-30 00:00:00
abstract：BACKGROUND:Children are scheduled to receive 18-20 immunizations before their 18th birthday in England and Wales; this approximates to 13 million vaccines administered per annum. Each immunization represents a potential opportunity for immunization-related error and effective immunization is imperative to maintain the ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2015.06.068
更新日期：2015-07-31 00:00:00
abstract：:The ability of ALVAC- or fowlpox-based simian immunodeficiency virus (SIV) vaccines to boost SIV-specific CD4+ and CD8+ T-cell responses was tested in 10 vaccinia-experienced macaques infected with SIVmac251. The CD8+ T-cell response to the dominant Gag(181-189) CM9 was quantitated in seven Mamu-A*01-positive macaques...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2003.08.028
更新日期：2004-01-26 00:00:00
abstract：OBJECTIVES:To assess the effectiveness and cost-effectiveness of routine childhood vaccination by new vaccines against rotavirus in France. METHODS:We constructed a Markov decision tree to compare two alternatives: "no vaccination" and "vaccination". A hypothetical birth cohort of 750,000 children was followed until 3...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.11.064
更新日期：2008-01-30 00:00:00
abstract：:Gamma inulin and Algammulin, two new adjuvants, were examined and compared with alum and Freund's Complete/Incomplete Adjuvant (FCA/FIA), for potentiation of cell-mediated immunity (CMI) and humoral immunity in sheep to a recombinant Taenia ovis antigen. The ability to protect sheep when challenged with live T. ovis e...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(94)00023-g
更新日期：1995-04-01 00:00:00
abstract：:Three vaccines, BCG alone, BCG + 10(7) killed Mycobacterium vaccae and 10(8) killed M. vaccae alone, were studied in children living in close contact with leprosy. In the year before vaccination, 14/446 (3.1%) children had developed leprosy. Among those who were not vaccinated, 9/74 (12.2%) developed the disease in th...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(01)00052-4
更新日期：2001-05-14 00:00:00
abstract：:Japanese encephalitis is a major cause of encephalitis in Asia. Cases occur largely in rural areas of the South and East Asian region resulting in significant morbidity and mortality. Multiple vaccines exist to control Japanese encephalitis, but all suffer from problems. Envelope protein domain III of Japanese encepha...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2009.09.006
更新日期：2009-11-16 00:00:00
abstract：INTRODUCTION:The Global Vaccine Action Plan (GVAP), unanimously endorsed by the World Health Assembly in 2012, defined an ambitious strategy to improve immunization. At the end of the decade, significant progress has been made but four of the five GVAP goals are likely to be missed. This report describes a set of surve...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2020.05.048
更新日期：2020-07-14 00:00:00
abstract：:Parainfluenza virus type 1 (PIV1) is a major cause of croup in infants and young children, and a vaccine is needed to prevent the serious disease caused by this virus. In the present study, a live attenuated PIV1 vaccine candidate was generated by modification of the extensively-studied PIV3 cold-passaged (cp) cp45 va...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(99)00227-3
更新日期：1999-10-14 00:00:00
abstract：:Intranasal inactivated influenza vaccines have the advantage over parenteral vaccines in that they are not associated with the pain of an injection. However, they would be most useful if they were available for all age groups, including high-risk groups, and also would provide cross-protection against variant virus st...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2010.05.019
更新日期：2010-08-31 00:00:00
