当前位置： SCI文献检索 > VACCINE期刊下所有文献 > Bacterially expressed recombinant envelope protein domain III of Japanese encephalitis virus (rJEV-DIII) elicits Th1 type of immune response in BALB/c mice.

Bacterially expressed recombinant envelope protein domain III of Japanese encephalitis virus (rJEV-DIII) elicits Th1 type of immune response in BALB/c mice.

Abstract：

:Japanese encephalitis is a major cause of encephalitis in Asia. Cases occur largely in rural areas of the South and East Asian region resulting in significant morbidity and mortality. Multiple vaccines exist to control Japanese encephalitis, but all suffer from problems. Envelope protein domain III of Japanese encephalitis virus is involved in binding to host receptors and it contains specific epitopes that elicit virus-neutralizing antibodies. Earlier, the protective efficacy of domain III has been evaluated in mice by some researchers, but these studies are lacking in explanation of humoral and cellular immune responses. We have earlier reported cloning, expression, purification and in vitro refolding of Japanese encephalitis virus envelope protein domain III (rJEV-DIII). Ninety percent JEV is neutralized when the serum against refolded rJEV-DIII is used at a dilution of 1:80. In the present study, we have evaluated the immunomodulatory potential of refolded rJEV-DIII protein in BALB/c mice with Freunds complete/incomplete adjuvants. Mice were tested for humoral immune response by ELISA. Cell-mediated immune response was tested by lymphocyte proliferation assay and cytokine profiling. The rJEV-DIII generated high IgG antibody and its isotypes (IgG2a and IgG3) and induced significant expression of INF-gamma and IL-2 cytokines. The rJEV-DIII induced significant lymphoproliferation of splenocytes. In conclusion rJEV-DIII induced Th1 type of immune response which plays an important role in protection for intracellular pathogens.

journal_name

Vaccine

journal_title

Vaccine

authors

Verma SK,Kumar S,Gupta N,Vedi S,Bhattacharya SM,Lakshmana Rao PV

doi

10.1016/j.vaccine.2009.09.006

subject

Has Abstract

pub_date

2009-11-16 00:00:00

pages

6905-9

issue

49

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(09)01330-9

journal_volume

27

pub_type

杂志文章

相关文献

VACCINE文献大全
  • Yellow fever vaccine: an updated assessment of advanced age as a risk factor for serious adverse events.

    abstract：:Since 1996, the scientific community has become aware of 14 reports of yellow fever vaccine (YEL)-associated viscerotropic disease (YEL-AVD) cases and four reports of YEL-associated neurotropic disease (YEL-AND) worldwide, changing our understanding of the risks of the vaccine. Based on 722 adverse event reports after...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2005.01.089

    authors： Khromava AY,Eidex RB,Weld LH,Kohl KS,Bradshaw RD,Chen RT,Cetron MS,Yellow Fever Vaccine Safety Working Group.

    更新日期：2005-05-09 00:00:00

  • Manifestations of systemic autoimmunity in vaccinated salmon.

    abstract：:The development of systemic autoimmunity may result as an undesired side-effect following vaccination, and this condition was recently shown to occur in farmed salmon (Salmo salar). Several of previously reported side-effects following vaccination of fish should therefore be reviewed in the light of this condition. He...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2010.05.032

    authors： Haugarvoll E,Bjerkås I,Szabo NJ,Satoh M,Koppang EO

    更新日期：2010-07-12 00:00:00

  • Critical overview and outlook: pathogenesis, prevention, and treatment of hepatitis and hepatocarcinoma caused by hepatitis B virus.

    abstract：:Viral hepatitis B is an enigmatic disease in which the host's own immune response to persistent viral infection may bring about host destruction through antiviral inflammatory responses which might otherwise present as a benign or inapparent disease. The simple solution to the hepatitis B problem is by immunoprophylax...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/s0264-410x(03)00529-2

    authors： Hilleman MR

    更新日期：2003-12-01 00:00:00

  • Using QPCR to assign infectious potencies to adenovirus based vaccines and vectors for gene therapy: toward a universal method for the facile quantitation of virus and vector potency.

    abstract：:The assignment of infectious potency to test articles of adenovirus has been conducted mainly using classical end-point dilution methods, which rely on virus induced cytopathology to reveal the presence of infectious virus. These assays suffer the disadvantages of labor intensity, duration, throughput restriction and ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2005.04.023

    authors： Wang F,Puddy AC,Mathis BC,Montalvo AG,Louis AA,McMackin JL,Xu J,Zhang Y,Tan CY,Schofield TL,Wolf JJ,Lewis JA

    更新日期：2005-08-22 00:00:00

  • Enhancement of intranasal vaccination with recombinant chain A ricin vaccine (rRV) in mice by the mucosal adjuvants LTK63 and LTR72.

    abstract：:Intranasal (i.n.) vaccination of mice with three doses of 40 microg of rRV stimulated low anti-ricin ELISA and neutralizing antibody responses, which were only marginally protective against aerosol-delivered 5-10 LD(50) of ricin toxin. To enhance the protection, and to reduce the lung injury of vaccinated mice that su...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2007.01.036

    authors： Kende M,Tan X,Wlazlowski C,Williams R,Lindsey C,Del Giudice G

    更新日期：2007-04-20 00:00:00

  • Identification of secreted proteins as novel antigenic vaccine candidates of Haemophilus parasuis serovar 5.

    abstract：:Haemophilus parasuis (H. parasuis) is a swine pathogen responsible for the Glässer's disease, which has received more attention in the past decade due to the increasing economic losses in the pig industry worldwide. As traditional inactive vaccine of H. parasuis has obvious disadvantage, to identify efficient immunopr...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2015.02.023

    authors： Li M,Song S,Yang D,Li C,Li G

    更新日期：2015-03-30 00:00:00

  • Rhesus macaques with high levels of vaccine induced IFN-gamma producing cells better control viral set-point following challenge with SIV239.

    abstract：:HIV-1 specific cellular immune responses play a significant part in controlling HIV-1 viral replication and are an important component of an HIV-1 vaccine induced immune response. We reported earlier that recombinant DNA vaccine delivered intramuscularly, and recombinant Listeria monocytogenes, delivered orally induce...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2005.08.016

    authors： Boyer JD,Maciag PC,Parkinson R,Wu L,Lewis MG,Weiner DB,Paterson Y

    更新日期：2006-05-22 00:00:00

  • Capacity for a global vaccine safety system: the perspective of national regulatory authorities.

    abstract：:Confidence in vaccine safety is critical to national immunization strategies and to global public health. To meet the Millenium Development Goals, and buoyed by the success of new vaccines produced in developing countries, the World Health Organization has been developing a strategy to establish a global system for ef...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.05.045

    authors： Graham JE,Borda-Rodriguez A,Huzair F,Zinck E

    更新日期：2012-07-13 00:00:00

  • Clinical experience with the meningococcal B vaccine, Bexsero(®): Prospects for reducing the burden of meningococcal serogroup B disease.

    abstract：:Although rare, invasive meningococcal disease remains an important cause of mortality and morbidity in children and young adults. Vaccines have been successfully introduced to help protect against meningococcal disease caused by serogroups A, C, W and Y, but until recently, a vaccine for serogroup B (MenB) was not ava...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/j.vaccine.2015.11.057

    authors： Watson PS,Turner DP

    更新日期：2016-02-10 00:00:00

  • Envelope proteins of two HIV-1 clades induced different epitope-specific antibody response.

    abstract：:Using HIV-1 envelope protein (Env)-based immunogens that closely mimic the conformation of functional HIV-1 Envs and represent the isolates prevalent in relevant geographical region is considered a rational approach towards developing HIV vaccine. We recently reported that like clade B Env, JRFL, membrane bound Indian...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.01.081

    authors： Shrivastava T,Samal S,Tyagi AK,Goswami S,Kumar N,Ozorowski G,Ward AB,Chakrabarti BK

    更新日期：2018-03-14 00:00:00

  • Enhancing immune responses to inactivated porcine parvovirus oil emulsion vaccine by co-inoculating porcine transfer factor in mice.

    abstract：:Inactivated porcine parvovirus (PPV) vaccines are available commercially and widely used in the breeding herds. However, inactivated PPV vaccines have deficiencies in induction of specific cellular immune response. Transfer factor (TF) is a material that obtained from the leukocytes, and is a novel immune-stimulatory ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.05.077

    authors： Wang RN,Wang YB,Geng JW,Guo DH,Liu F,Chen HY,Zhang HY,Cui BA,Wei ZY

    更新日期：2012-07-27 00:00:00

  • Interpretation of primary care physicians' attitude regarding rotavirus immunisation using diffusion of innovation theories.

    abstract：:To evaluate primary care physicians' attitude towards implementation of rotavirus (RV) immunisation into the Swiss immunisation schedule, an eight-question internet-based questionnaire was sent to the 3799 subscribers of InfoVac, a nationwide web-based expert network on immunisation issues, which reaches >95% of paedi...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.05.097

    authors： Agyeman P,Desgrandchamps D,Vaudaux B,Berger C,Diana A,Heininger U,Siegrist CA,Aebi C

    更新日期：2009-07-30 00:00:00

  • Shedding of live vaccine virus, comparative safety, and influenza-specific antibody responses after administration of live attenuated and inactivated trivalent influenza vaccines to HIV-infected children.

    abstract：:HIV-infected children (N=243), >or=5 to <18 years old, receiving stable antiretroviral therapy, were stratified by immunologic status and randomly assigned to receive intranasal live attenuated influenza vaccine (LAIV) or intramuscular trivalent inactivated influenza vaccine (TIV). The safety profile after LAIV or TIV...

    journal_title：Vaccine

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.vaccine.2008.05.054

    authors： Levin MJ,Song LY,Fenton T,Nachman S,Patterson J,Walker R,Kemble G,Allende M,Hultquist M,Yi T,Nowak B,Weinberg A

    更新日期：2008-08-05 00:00:00

  • Racial disparities in diarrhea-associated hospitalizations among children in five US States, before and after introduction of rotavirus vaccine.

    abstract：:Racial differences in diarrheal disease have not been systematically examined, and the impact of rotavirus vaccine on these differences has not been assessed. We compared diarrhea-associated hospitalizations by race/ethnicity among children <5 years pre- (2000-2006) and post- (2007 and 2008) rotavirus vaccine introduc...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2010.08.094

    authors： Yen C,Steiner CA,Barrett M,Curns AT,Hunter K,Wilson E,Parashar UD

    更新日期：2010-10-28 00:00:00

  • Co-administration of attenuated Mycoplasma hyopneumoniae 168 strain with bacterial DNA enhances the local and systemic immune response after intranasal vaccination in pigs.

    abstract：:Mycoplasma hyopneumoniae, the primary pathogen of enzootic pneumonia, occurs worldwide and causes major economic losses to the pig industry. M. hyopneumoniae infects pigs at mucosal surfaces of respiratory tract. The aim of the present study was to investigate if the protection rate against M. hyopneumoniae infection ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.01.028

    authors： Li Y,Li P,Wang X,Yu Q,Yang Q

    更新日期：2012-03-09 00:00:00

  • No major outbreaks of pseudorabies virus in well-immunized sow herds.

    abstract：:In this study we quantified the transmission of pseudorabies virus (PRV) in well-immunized sow herds in The Netherlands. In three herds, sows were tested for antibodies to gE of PRV every time after they had been transported to another barn (survey A). In 99 other herds, sows were tested simultaneously once or twice y...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/0264-410x(96)00022-9

    authors： Van Nes A,Stegeman JA,De Jong MC,Loeffen WL,Kimman TG,Verheijden JH

    更新日期：1996-08-01 00:00:00

  • Poly(propyleneimine) dendrimer and dendrosome mediated genetic immunization against hepatitis B.

    abstract：:The purpose of the present research work is to explore the potential of dendrosomes in genetic immunization against hepatitis B. Plasmid DNA encoding pRc/CMV-HBs[S] (5.6 kb), encoding the small region of the hepatitis B surface antigen, was complexed with 5th generation poly(propyleneimine) dendrimer (PPI) in differen...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2008.04.058

    authors： Dutta T,Garg M,Jain NK

    更新日期：2008-06-25 00:00:00

  • Pertussis immunization in paediatric healthcare workers: knowledge, attitudes, beliefs, and behaviour.

    abstract：:Healthcare workers' (HCWs) knowledge, attitudes, and beliefs regarding pertussis immunization were assessed and compared to the rate of vaccine uptake. A questionnaire was distributed to employees at a paediatric and maternity tertiary care centre. Respondents were then offered a dose of the tetanus, diphtheria, and a...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.12.060

    authors： Top KA,Halperin BA,Baxendale D,MacKinnon-Cameron D,Halperin SA

    更新日期：2010-03-02 00:00:00

  • Chapter 15: HPV vaccine use in the developing world.

    abstract：:Human papillomavirus (HPV)-related morbidity and mortality from cervical cancer primarily occurs in the developing world, where, unfortunately, access to vaccines in general, and expensive newer vaccines in particular, is often more limited than in the industrialized world. In addition, secondary prevention methods su...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/j.vaccine.2006.05.128

    authors： Kane MA,Sherris J,Coursaget P,Aguado T,Cutts F

    更新日期：2006-08-31 00:00:00

  • Evaluation of immunization injection safety in China, 2010: achievements, future sustainability.

    abstract：OBJECTIVE:The study objectives were to evaluate injection practices in China in the post GAVI project era and provide guidance for policy makers to update national standards for injection practices and further improve vaccination services. METHODS:We conducted a national stratified, cross-sectional survey in October 2...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.11.057

    authors： Wu Z,Cui F,Chen Y,Miao N,Gong X,Luo H,Wang F,Zheng H,Kane M,Hadler SC,Hutin YJ,Liang X,Yang W

    更新日期：2013-12-27 00:00:00

  • Pandemic influenza H1N1 2009 infection in Victoria, Australia: no evidence for harm or benefit following receipt of seasonal influenza vaccine in 2009.

    abstract：:Conflicting findings regarding the level of protection offered by seasonal influenza vaccination against pandemic influenza H1N1 have been reported. We performed a test-negative case control study using sentinel patients from general practices in Victoria to estimate seasonal influenza vaccine effectiveness against la...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2011.03.055

    authors： Kelly HA,Grant KA,Fielding JE,Carville KS,Looker CO,Tran T,Jacoby P

    更新日期：2011-08-26 00:00:00

  • Intramuscular delivery of a cholera DNA vaccine primes both systemic and mucosal protective antibody responses against cholera.

    abstract：:Cholera is a potentially lethal diarrhea disease caused by the gram-negative bacterium Vibrio cholerae. The need for an effective cholera vaccine is clearly indicated but the challenges of eliciting both systemic and mucosal immune responses remains a significant challenge. In the current report, we discovered that a ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.04.008

    authors： Xu G,Wang S,Zhuang L,Hackett A,Gu L,Zhang L,Zhang C,Wang H,Huang Z,Lu S

    更新日期：2009-06-12 00:00:00

  • Pediatric immunization-related safety incidents in primary care: A mixed methods analysis of a national database.

    abstract：BACKGROUND:Children are scheduled to receive 18-20 immunizations before their 18th birthday in England and Wales; this approximates to 13 million vaccines administered per annum. Each immunization represents a potential opportunity for immunization-related error and effective immunization is imperative to maintain the ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2015.06.068

    authors： Rees P,Edwards A,Powell C,Evans HP,Carter B,Hibbert P,Makeham M,Sheikh A,Donaldson L,Carson-Stevens A

    更新日期：2015-07-31 00:00:00

  • Envelope protein and p18(IIIB) peptide recognized by cytotoxic T lymphocytes from humans immunized with human immunodeficiency virus envelope.

    abstract：:Cytotoxic T cells are the main antigen-specific effector cells of the cellular immune system and MHC class I restricted cytotoxic T-lymphocyte (CTL) responses in mice, acting against the HIV-1 envelope protein, are known to be predominantly directed against an amino acid sequence in the third hypervariable domain. We ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/0264-410x(93)90251-r

    authors： Achour A,Picard O,Mbika JP,Willer A,Snart R,Bizzini B,Carelli C,Burny A,Zagury D

    更新日期：1993-01-01 00:00:00

  • Influence of adjuvant-active peptidoglycan monomer on specific T cell responses in mice.

    abstract：:Peptidoglycan monomer (PGM) originating from Brevibacterium divaricatum is a non-toxic, non-pyrogenic, water-soluble immunostimulator. It potentiates humoral immune response to ovalbumin (OVA) in mice upregulating both immunoglobulin (IgG) 1 and IgG2a antibody subclasses. This study concerns the influence of PGM on T ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(02)00336-5

    authors： Halassy Spoljar B,Cimbora T,Hanzl-Dujmović I,Dojnović B,Sabioncello A,Krstanović M,Tomasić J

    更新日期：2002-10-04 00:00:00

  • Global programme for control of hepatitis B infection.

    abstract：:The hepatitis B virus (HBV) has infected more than 2000 million persons alive today and 350 million persons are chronically infected carriers of the virus, at high risk of death from active hepatitis, cirrhosis and primary hepatocellular cancer. Each year approximately 1 million people die from the acute and chronic s...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/0264-410x(95)80050-n

    authors： Kane M

    更新日期：1995-01-01 00:00:00

  • Comparative analysis of immunization schedules using a novel adenovirus-based immunotherapeutic targeting hepatitis B in naïve and tolerant mouse models.

    abstract：:Development of active targeted immunotherapeutics is a rapid developing field in the arena of chronic infectious diseases. The question of repeated, closely spaced administration of immunotherapeutics to achieve a rapid impact on the replicating agent is an important one. We analyzed here, using a prototype adenovirus...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2014.03.089

    authors： Boukhebza H,Dubois C,Koerper V,Evlachev A,Schlesinger Y,Menguy T,Silvestre N,Riedl P,Inchauspé G,Martin P

    更新日期：2014-05-30 00:00:00

  • Role of the virology diagnosis laboratory in the surveillance of rubella virus Cuba 1988/2000.

    abstract：:In Cuba, on the basis of Measles Elimination Program, the incidence of this disease decline, and was necessary to test rubella virus as a possible etiology agent that produce fever and rash illness. To reach this goal, Cuba developed rubella elimination strategies with integrated epidemiologic and laboratory surveilla...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2004.04.023

    authors： de Los Angeles Ribas M,Galindo M,Torres G,Valcarsel M,Cancio R,Guzmán MG,Rosario D,Resik S,Rodriguez C,Garcia D,Tejero Y

    更新日期：2004-10-22 00:00:00

  • The feasibility of identifying children with primary immunodeficiency disorders: preparation for the polio post-eradication era in Bangladesh.

    abstract：BACKGROUND:Persons with primary immunodeficiency disorders (PIDD) who receive oral poliovirus vaccine (OPV) or are household contacts of OPV recipients are at risk of excreting immunodeficiency-associated vaccine-derived polioviruses (iVDPVs). iVDPVs can be transmitted and cause paralytic polio. The objective of this s...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.06.017

    authors： Sazzad HM,Rainey JJ,Mach O,Sutter R,Diordista S,Kawser CA,Mobarak R,Alam D,Chowdhury MA,Hossain MJ,Hasan AS,Luby SP

    更新日期：2012-08-03 00:00:00

  • Impact of universal vaccination programmes on the epidemiology of hepatitis B: 10 years of experience in Italy.

    abstract：:Ten years have elapsed since routine vaccination of infants and of 12-year-old adolescent was implemented in Italy. In this period, evidence has accumulated on the epidemiological impact of universal immunisation. Coverage is on average >90% and is >or=95% in many areas of the country. Incidence of acute hepatitis B, ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(02)00580-7

    authors： Bonanni P,Pesavento G,Bechini A,Tiscione E,Mannelli F,Benucci C,Nostro AL

    更新日期：2003-01-30 00:00:00