Abstract:
:Development of active targeted immunotherapeutics is a rapid developing field in the arena of chronic infectious diseases. The question of repeated, closely spaced administration of immunotherapeutics to achieve a rapid impact on the replicating agent is an important one. We analyzed here, using a prototype adenovirus-based immunotherapeutic encoding Core and Polymerase from the hepatitis B virus (Ad-HBV), the influence of closely spaced repeated immunizations on the level and quality of induced HBV-specific and vector-specific immune responses in various mouse models. Ad-HBV, whether injected once or multiple times, was able to induce HBV- and adeno-specific T cells both in HBV-free mice and in a HBV tolerant mouse model. Adenovirus-specific T cell responses and titers of neutralizing anti-Ad5 antibodies increased from time of the 3rd injection. Interestingly, single or multiple Ad-HBV injections resulted in detection of Polymerase-specific functional T cells in HBV tolerant mice. Overall no modulation of the levels of HBV-specific cytokine-producing (IFNγ/TNFα) and cytolytic T cells was observed following repeated administrations (3 or 6 weekly injections) when compared with levels detected after a single injection with the exception of two markers: 1. the proportion of HBV-specific IFNγ-producing cells bearing the CD27+/CD43+ phenotype appeared to be sustained in C57BL/6J mice following 6 weekly injections; 2. the percentage of IFNγ/TNFα Core-specific producing cells observed in spleens of HLA-A2 mice as well as of that specific of Polymerase observed in livers of HBV tolerant mice was maintained. In addition, percentage of HBV-specific T cells expressing PD-1 was not increased by multiple injections. Overall these data show that, under experimental conditions used, rapid, closely spaced administrations of an adenovirus-based HBV immunotherapeutics does not inhibit induced T-cell responses including in a HBV-tolerant environment.
journal_name
Vaccinejournal_title
Vaccineauthors
Boukhebza H,Dubois C,Koerper V,Evlachev A,Schlesinger Y,Menguy T,Silvestre N,Riedl P,Inchauspé G,Martin Pdoi
10.1016/j.vaccine.2014.03.089subject
Has Abstractpub_date
2014-05-30 00:00:00pages
3256-63issue
26eissn
0264-410Xissn
1873-2518pii
S0264-410X(14)00480-0journal_volume
32pub_type
杂志文章相关文献
VACCINE文献大全abstract::Helicobacter pylori is a Gram-negative bacterial pathogen associated with gastritis, peptic ulceration, and gastric carcinoma. The bacteria express a strong urease activity which is known to be essential for colonization of gnotobiotic pigs and nude mice. UreA and UreB, two structural subunits of the active enzyme, we...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(97)00247-8
更新日期:1998-03-01 00:00:00
abstract::Human adenoviruses (AdVs) have been extensively studied as vectors for gene therapy and vaccination. However, little attention has been paid to AdV vaccine development and treatment. Currently, there is a lack of information concerning the immunogenicity of AdV major capsid proteins. Here, using AdV7 as a model, we co...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.05.118
更新日期:2018-07-05 00:00:00
abstract::The Middle East respiratory syndrome coronavirus (MERS-CoV) is a highly pathogenic and zoonotic virus with a fatality rate in humans of over 35%. Although several vaccine candidates have been developed, there is still no clinically available vaccine for MERS-CoV. In this study, we developed two types of MERS-CoV vacci...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.04.082
更新日期:2018-06-07 00:00:00
abstract::Pneumococcal conjugate vaccines (PCV) may reduce nasopharyngeal carriage (NPC) of Streptococcus pneumoniae vaccine strains (VT), but serotype replacement with non-vaccine strains (NVT) has been reported. Bacterial NPC after PHiD-CV vaccination was assessed in the second year of life. Open descriptive study of NPC repo...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.12.086
更新日期:2011-02-24 00:00:00
abstract::We previously reported the development of an oral vaccine for diabetes based on live attenuated Salmonella-expressing preproinsulin (PPI) as the autoantigen. When combined with host cell-expressed TGFβ, the vaccine prevented the onset of diabetes in non-obese diabetic (NOD) mice. Herein, we investigated factors that c...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.10.101
更新日期:2018-12-18 00:00:00
abstract::Mutations arising in neutralizing epitopes of hepatitis C virus may play a role in the ability of the virus to escape control by neutralizing antibodies and in the establishment of chronic infections. An amino-acid substitution, Q412H, within a major conserved neutralization epitope EP I (aa 412-426) in the E2 glycopr...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.04.024
更新日期:2010-06-07 00:00:00
abstract::Cattle produce specific serum antibody mainly of the IgG1 isotype in response to infection with the liver fluke, Fasciola hepatica. In these animals a positive correlation between fluke-specific serum IgG1 levels and fluke-burden in non-immunized infected animals was observed. In contrast, immunization of cattle with ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(97)00289-2
更新日期:1998-05-01 00:00:00
abstract::While the large majority of parents in the U.S. vaccinate their children according to the recommended immunization schedule, some parents have refused or delayed vaccinating, often citing safety concerns. In response to public concern, the U.S. Institute of Medicine (IOM) evaluated existing research regarding the safe...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.10.082
更新日期:2016-02-15 00:00:00
abstract:INTRODUCTION:Maternal and childhood vaccine decision-making begins prenatally. Amongst pregnant Australian women we aimed to ascertain vaccine information received, maternal immunisation uptake and attitudes and concerns regarding childhood vaccination. We also aimed to determine any correlation between a) intentions a...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.08.003
更新日期:2018-10-22 00:00:00
abstract:INTRODUCTION:Institutionalized mentally retarded subjects are well-known to be at-risk for HBV infection. We studied the persistence of vaccine-induced anti-HBs antibodies and the robustness of the HBsAg-specific immune memory in this population, 18-20 years after the first vaccine dose. MATERIALS AND METHODS:Non-immu...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.05.044
更新日期:2012-07-06 00:00:00
abstract::Bacterial infectious diseases remain the single most important threat to health worldwide. Although conventional vaccinology approaches were successful in conferring protection against several diseases, they failed to provide efficacious solutions against many others. The advent of whole-genome sequencing changed the ...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2009.01.072
更新日期:2009-05-26 00:00:00
abstract::The target animals and vaccination regimes for vaccines against the bovine rhinotracheitis (IBR) and the bovine viral diarrhoea virus (BVDV) are very similar. Therefore, we have compared different schedules for the combined use of a live IBR marker vaccine and an inactivated BVD vaccine. The neutralizing antibody resp...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.06.050
更新日期:2007-09-04 00:00:00
abstract::Due to viral strains, influenza season, and consultations and admission rates varying from country to country, the continued economic evaluation of influenza vaccination for the elderly people aged 65 years and above is paramount, particularly in areas with dense population. Efficacy and cost-effective analysis of inf...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.10.011
更新日期:2005-03-14 00:00:00
abstract:BACKGROUND:To better understand how to prevent and respond to pneumococcal meningitis outbreaks in the meningitis belt, we retrospectively examined Burkina Faso's case-based meningitis surveillance data for pneumococcal meningitis clusters and assessed potential usefulness of response strategies. METHODS:Demographic a...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.06.002
更新日期:2020-07-31 00:00:00
abstract:BACKGROUND AND AIMS:Trans-placentally acquired antibodies can protect infants from infection in the first months of life. However, high concentrations of antibody at birth may impact the infant's own immune response to primary immunization. We examine the relationship between concentration of specific antibody to Borde...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.11.104
更新日期:2014-02-12 00:00:00
abstract:BACKGROUND:Household-based caregivers serve an important role in protecting the health of cancer patients, who may be vulnerable to infectious diseases due to their cancer treatments. Caregiver preventive care should be prioritized to maintain continuity of care and to reduce potential for transmission of infectious di...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.07.052
更新日期:2020-09-11 00:00:00
abstract::The greatest risk from live-attenuated vaccines is reversion to virulence. Particular concerns arise for RNA viruses, which exhibit high mutation frequencies. We examined the stability of 3 attenuation strategies for the alphavirus, Venezuelan equine encephalitis virus (VEEV): a traditional, point mutation-dependent a...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.01.055
更新日期:2011-03-09 00:00:00
abstract::Randomized controlled clinical trials represent the best way to establish the therapeutic or preventive value of medicines. This decade has seen a strong shift in the location of clinical trials from industrialized countries to developing countries, including many in Africa. However, without independent strong regulat...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.08.113
更新日期:2009-12-09 00:00:00
abstract:BACKGROUND:One of the most concerning public health issues, related to vaccination and disease prevention, is the inability to induce durable immune responses following a single-dose immunization. In this regard, the nature of the inflammatory environment induced by vaccine adjuvants can negatively impact the resulting...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.04.004
更新日期:2019-05-06 00:00:00
abstract::Human papillomavirus (HPV) infection causes cervical cancer and genital warts. Young women (1106) were randomized to receive one of three formulations of a quadrivalent HPV (Types 6/11/16/18) L1 virus-like particle (VLP) vaccine or one of two placebo formulations. The goal was to assess vaccine safety and immunogenici...
journal_title:Vaccine
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.vaccine.2006.04.068
更新日期:2006-07-07 00:00:00
abstract::The effect of liposome encapsulation on the antibody response to bovine serum albumin (BSA), human carcinoembryonic antigen (CEA) and sheep IgG (sIgG) has been determined in the mouse. Dipalmitoylphosphatidylcholine/dimyristolylphosphatidylglycerol liposomes (10:1 molar ratio; 1 mumol) containing BSA, CEA or sIgG indu...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(92)90004-4
更新日期:1992-01-01 00:00:00
abstract::A randomized, double-blind, controlled clinical trial was conducted to evaluate the efficacy and safety of CJ-50300, a newly developed cell culture-derived smallpox vaccine, and to determine its minimum effective dose. The overall rates of cutaneous "take" reaction and humoral and cellular immunogenicity in CJ-50300 v...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2010.06.063
更新日期:2010-08-16 00:00:00
abstract::The success of immunization programs in lowering the incidence of vaccine preventable diseases (VPDs) has led to increased public attention on potential health risks associated with vaccines. As a result, a scientifically rigorous response to investigating reported adverse events following immunization (AEFI) and effe...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2019.07.041
更新日期:2020-11-17 00:00:00
abstract::The membrane proximal region (MPR) of HIV-1 gp41 is a desirable target for development of a vaccine that elicits neutralizing antibodies since the patient-derived monoclonal antibodies, 2F5 and 4E10, bind to the MPR and neutralize primary HIV isolates. The 2F5 and 4E10 antibodies cross-react with lipids and structural...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.05.059
更新日期:2009-07-23 00:00:00
abstract::We estimated the sensitivity, i.e., the proportion of all cases of adverse events following immunization (AEFIs) reported to the Brazilian passive surveillance for adverse events following immunization (PSAEFI) with the diphtheria-tetanus-whole-cell pertussis-Haemophilus influenzae type b (DTwP/Hib) vaccine, as well a...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.02.059
更新日期:2010-04-19 00:00:00
abstract::The aim of this study was to determine the rubella seroprevalence in women of reproductive age. This cross-sectional study was conducted on women aged 15-49 years. Serologic studies were performed by using the ELISA method. The number of women enrolled in the study was 607, the mean age was 30.7+/-9.3 years and 13.2% ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.09.033
更新日期:2007-01-15 00:00:00
abstract::The carboxyl-terminal region of the merozoite surface protein-1 (MSP1) is a leading candidate for a vaccine against malaria in the erythrocytic stage. In this study, we investigated the utility of interleukin-12 (IL-12) cDNA as an adjuvant for malaria DNA vaccine in a mouse challenge model. We found that co-immunizati...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(02)00665-5
更新日期:2003-03-28 00:00:00
abstract::Influenza B virus infections are mainly restricted to humans, which is partially caused by the inability of influenza B virus NS1 protein to counteract the innate immune response of other species. However, for cell culture-based influenza vaccine production non-human cells, such as Madin-Darby canine kidney (MDCK) cel...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.05.069
更新日期:2011-09-22 00:00:00
abstract::This study was designed to evaluate the immunogenicity of autoclaved and nonautoclaved preparations of a vaccine composed of whole antigens from killed promastigotes of Leishmania amazonensis. Leishmanin skin-test (LST)-negative volunteers were immunized with either autoclaved or nonautoclaved vaccine preparations (32...
journal_title:Vaccine
pub_type: 临床试验,杂志文章
doi:10.1016/s0264-410x(98)00338-7
更新日期:1999-03-05 00:00:00
abstract::Swine influenza virus (SIV) is not only an important respiratory pathogen in pigs but also a potent threat to human health. Although immunization with recombinant poxviruses expressing protective antigens as vaccines has been widely used for against many infectious diseases, development of recombinant swinepox virus (...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.02.028
更新日期:2012-04-26 00:00:00