当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > High-active truncated luciferase of copepod Metridia longa.

High-active truncated luciferase of copepod Metridia longa.

Abstract：

:The technology of real-time imaging in living cells is crucial for understanding of intracellular events. For this purpose, bioluminescent reporters have been introduced as sensitive and convenient tools. Metridia luciferase (MLuc) from the copepod Metridia longa is a coelenterazine-dependent luciferase containing a natural signal peptide for secretion. We report the high-active MLuc mutants with deletion of the N-terminal variable part of amino acid sequence. The MLuc variants were produced in Escherichia coli cells, converted to an active protein, and characterized. We demonstrate that the truncated MLucs have significantly increased bioluminescent activity as against the wild type enzyme but substantially retain other properties. One of the truncated variants of MLuc was transiently expressed in HEK 293 cells. The results clearly suggest that the truncated Metridia luciferase is well suited as a secreted reporter ensuring higher detection sensitivity in comparison with a wild type enzyme.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Markova SV,Burakova LP,Vysotski ES

doi

10.1016/j.bbrc.2011.11.063

subject

Has Abstract

pub_date

2012-01-06 00:00:00

pages

98-103

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(11)02077-8

journal_volume

417

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Identification of porin as a binding site for MAP2.

    abstract：:Porin or VDAC, the pore forming protein in the outer mitochondrial membrane, was identified as a binding site for microtubule-associated protein 2 (MAP2). By affinity chromatography using covalently bound MAP2, two proteins were selectively purified with molecular weights 63 and 70 KD. The 63 KD protein was identified...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0148

    authors： Lindén M,Karlsson G

    更新日期：1996-01-26 00:00:00

  • Effect of aging and acetyl-L-carnitine on the lipid composition of rat plasma and erythrocytes.

    abstract：:The effect of aging and treatment with acetyl-L-carnitine on the lipid composition of rat plasma and erythrocytes was studied. It was found that aging increases the levels of free and esterified cholesterol. Fatty acid patterns in the plasma of aged rats show remarkable alterations when compared with control rats. The...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)92137-o

    authors： Ruggiero FM,Cafagna F,Gadaleta MN,Quagliariello E

    更新日期：1990-07-31 00:00:00

  • Genistein, a tyrosine kinase inhibitor, blocks the cell cycle progression but not Ca2+ influx induced by BAY K8644 in FRTL-5 cells.

    abstract：:IGF-I, when added to TSH-primed FRTL-5 cells, induces a long lasting Ca2+ influx and then DNA synthesis. Ca2+ channel agonist, BAYK 8644, is capable to mimic these effects on cell proliferation. We studied the effect of genistein, a specific tyrosine kinase inhibitor, on BAY K8644- or IGF-I- induced cell cycle progres...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1120

    authors： Takano T,Takada K,Tada H,Nishiyama S,Amino N

    更新日期：1993-02-15 00:00:00

  • TTX resistivity of Na+ channel in newt retinal neuron.

    abstract：:We examined voltage-dependent, TTX-resistant Na+ channels of newt retina (nRNaCh) electrophysiologically. IC50-TTX value of nRNaCh is more than 20 microM. We determined partial cDNA sequences of nRNaCh restricted to TTX binding sites (SS2 regions of all four repeats). While the amino acid sequences of SS2 regions of r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7696

    authors： Kaneko Y,Matsumoto G,Hanyu Y

    更新日期：1997-11-26 00:00:00

  • Let7a involves in neural stem cell differentiation relating with TLX level.

    abstract：:Neural stem cells (NSCs) have the potential for differentiation into neurons known as a groundbreaking therapeutic solution for central nervous system (CNS) diseases. To resolve the therapeutic efficiency of NSCs, recent researchers have focused on the study on microRNA's role in CNS. Some micro RNAs have been reporte...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.05.004

    authors： Song J,Cho KJ,Oh Y,Lee JE

    更新日期：2015-07-10 00:00:00

  • Spin-trapping of superoxide ion by a water-soluble, nitroso-aromatic spin-trap.

    abstract：:Spin-trapping of superoxide ion, O2-, which is produced from two different sources (OH(-)-DMSO and xanthine-xanthine oxidase systems), was investigated by use of a water-soluble, notroso-aromatic spin trap, sodium 3,5-dibromo-4-nitrosobenzene-sulfonate (DBNBS). It was found that O2- from all sources was easily trapped...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90491-2

    authors： Ozawa T,Hanaki A

    更新日期：1986-04-29 00:00:00

  • Genomic analyses and expression evaluation of thaumatin-like gene family in the cacao fungal pathogen Moniliophthora perniciosa.

    abstract：:Thaumatin-like proteins (TLPs) are found in diverse eukaryotes. Plant TLPs, known as Pathogenicity Related Protein (PR-5), are considered fungal inhibitors. However, genes encoding TLPs are frequently found in fungal genomes. In this work, we have identified that Moniliophthora perniciosa, a basidiomycete pathogen tha...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.09.054

    authors： Franco Sde F,Baroni RM,Carazzolle MF,Teixeira PJ,Reis O,Pereira GA,Mondego JM

    更新日期：2015-10-30 00:00:00

  • 2'-Amino-2'-deoxyguanosine is a cofactor for self-splicing in group I catalytic RNA.

    abstract：:In investigations on self-splicing in the group I intron of the pre-mRNA from the nrdB gene of bacteriophage T4 it was found that 2'-amino-2'-deoxyguanosine can replace guanosine as cofactor. This is the first guanosine-analogue with a modification in the 2'-position and substantial activity in a group I self-splicing...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)90560-8

    authors： Strömberg R,Hahne S,Sjögren AS,Sjöberg BM

    更新日期：1992-03-16 00:00:00

  • Identification and characterization of a novel gene EOLA1 stimulating ECV304 cell proliferation.

    abstract：:To study the changes in gene expression in endothelial cells stimulated by lipopolysaccharide (LPS) we performed subtraction hybridization on control human umbilical vein endothelial cells (HUVEC) versus HUVEC stimulated by LPS. A novel cDNA, named endothelial-overexpressed lipopolysaccharide-associated factor 1 (EOLA...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.10.124

    authors： Liang Z,Yang Z

    更新日期：2004-12-17 00:00:00

  • Cell polarization as a possible mechanism of response termination.

    abstract：:Neutrophils stimulated with a low concentration of chemotactic peptide N-formyl-met-leu-phe respond with a brief pulse of actin polymerization and a persistent change in morphology from round to polarized. The recovery of the actin polymerization response is unlikely to be due to usual receptor desensitization mechani...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1058

    authors： Model MA,Omann GM

    更新日期：1996-07-16 00:00:00

  • E-series prostaglandins activate cAMP-mediated potassium currents in follicle-enclosed Xenopus oocyte.

    abstract：:Receptor-mediated responses to prostaglandins E1 and E2 are shown by electrophysiological methods in follicle-enclosed oocytes of Xenopus laevis. In voltage-clamped oocytes, prostaglandins E1 and E2 elicited an outward hyperpolarizing current. This outward membrane current was caused by an increase in K+ conductance. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)90849-8

    authors： Mori K,Oka S,Tani A,Ito S,Watanabe Y

    更新日期：1989-08-15 00:00:00

  • Reversible beta-pleated sheet formation of a phosphorylated synthetic tau peptide.

    abstract：:Serine416 of human tau protein is believed to be phosphorylated in Alzheimer neurofibrillary tangles. We synthesized a fragment of tau, consisting of amino acids 408-421 in both non-phosphorylated and serine416-phosphorylated forms. Circular dichroism in a trifluoroethanol-water mixture indicated a beta-turn----beta-p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80112-3

    authors： Lang E,Szendrei GI,Elekes I,Lee VM,Otvos L Jr

    更新日期：1992-01-15 00:00:00

  • Evidence for the presence of specific high affinity cytosolic binding sites for platelet-activating factor in human neutrophils.

    abstract：:In this study evidence for the presence of specific cytosolic platelet-activating factor (PAF) binding sites has been presented. The equilibrium dissociation constant (Kd) as determined by Scatchard analysis was 4.29 x 10(-9) M for the cytosolic and 3.71 x 10(-9) M for the membrane fraction. The maximal number of bind...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1025

    authors： Svetlov S,Nigam S

    更新日期：1993-01-15 00:00:00

  • Loss of MURC/Cavin-4 induces JNK and MMP-9 activity enhancement in vascular smooth muscle cells and exacerbates abdominal aortic aneurysm.

    abstract：:Abdominal aortic aneurysm (AAA) is relatively common in elderly patients with atherosclerosis. MURC (muscle-restricted coiled-coil protein)/Cavin-4 modulating the caveolae function of muscle cells is expressed in cardiomyocytes, skeletal muscle cells and smooth muscle cells. Here, we show a novel functional role of MU...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.04.096

    authors： Miyagawa K,Ogata T,Ueyama T,Kasahara T,Nakanishi N,Naito D,Taniguchi T,Hamaoka T,Maruyama N,Nishi M,Kimura T,Yamada H,Aoki H,Matoba S

    更新日期：2017-06-03 00:00:00

  • Melittin-GM1 interaction: a model for a side-by-side complex.

    abstract：:We report here the interaction of melittin with ganglioside GM1 by steady-state fluorescence, one-dimensional (1)H NMR spectroscopy and molecular modeling. In the presence of GM1 the emission maximum of melittin is blue shifted and fluorescence quenching efficiencies of iodide and acrylamide are substantially reduced,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6684

    authors： Chatterjee C,Mukhopadhyay C

    更新日期：2002-03-29 00:00:00

  • Perillaldehyde attenuates cerebral ischemia-reperfusion injury-triggered overexpression of inflammatory cytokines via modulating Akt/JNK pathway in the rat brain cortex.

    abstract：:Perillaldehyde (PAH), one of the major oil components in Perilla frutescens, has anti-inflammatory effects. Few studies have examined the neuroprotective effect of PAH on stroke. So the aim of our study is to investigate the effect of PAH on ischemia-reperfusion-induced injury in the rat brain cortex. Middle cerebral ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.10.025

    authors： Xu L,Li Y,Fu Q,Ma S

    更新日期：2014-11-07 00:00:00

  • Binding of two zinc finger nuclease monomers to two specific sites is required for effective double-strand DNA cleavage.

    abstract：:Custom-designed zinc finger nucleases (ZFNs) are becoming powerful tools in gene targeting-the process of replacing a gene within a genome by homologous recombination. Here, we have studied the DNA cleavage by one such ZFN, DeltaQNK-FN, in order to gain insight into how ZFNs cleave DNA and how two inverted sites promo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.07.021

    authors： Mani M,Smith J,Kandavelou K,Berg JM,Chandrasegaran S

    更新日期：2005-09-09 00:00:00

  • Niosomal virosome derived by vesicular stomatitis virus glycoprotein as a new gene carrier.

    abstract：:Virosomes as membranous vesicles with viral fusion protein in their membrane are versatile vehicles for cargo delivery. The vesicular stomatitis virus glycoprotein (VSV-G) is a common fusogenic protein used in virosome preparation. This glycoprotein has been used in liposomal systems so far, but in this study, we have...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.10.054

    authors： Asadikaram G,Poustforoosh A,Pardakhty A,Torkzadeh-Mahani M,Nematollahi MH

    更新日期：2021-01-01 00:00:00

  • Nitrosative stress on yeast: inhibition of glyoxalase-I and glyceraldehyde-3-phosphate dehydrogenase in the presence of GSNO.

    abstract：:Under nitrosative stressed condition intracellular GSNO accumulation is common to all cell types. Conserved NADH-dependent GSNO reductase was reported previously as an important cellular protective measure against this. In spite of the constitutive nature of the enzyme, we observed in vivo inactivation of two importan...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00251-1

    authors： Sahoo R,Sengupta R,Ghosh S

    更新日期：2003-03-21 00:00:00

  • Mutational and inhibitive analysis of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer-based assays.

    abstract：:The 3C-like protease (3CL(pro)) of severe acute respiratory syndrome coronavirus (SARS-CoV) plays key roles in viral replication and is an attractive target for anti-SARS drug discovery. In this report, a fluorescence resonance energy transfer (FRET)-based method was developed to assess the proteolytic activity of SAR...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.04.072

    authors： Kuang WF,Chow LP,Wu MH,Hwang LH

    更新日期：2005-06-17 00:00:00

  • Heat shock induces phosphorylation of histone H2AX in mammalian cells.

    abstract：:Heat shock induces a variety of biological events including gene activation, cell cycle arrest, and apoptosis. Heat shock has recently been shown to be potentially useful when combined with radiation in cancer therapy, probably because, in mammalian cells, heat inhibits the repair of double-strand breaks (DSBs) induce...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.01.073

    authors： Kaneko H,Igarashi K,Kataoka K,Miura M

    更新日期：2005-03-25 00:00:00

  • HASH-1 and E2-2 are expressed in human neuroblastoma cells and form a functional complex.

    abstract：:The basic helix-loop-helix (bHLH) transcription factor mammalian achaete-scute homolog-1 (MASH-1 in mouse and HASH-1 in human) is essential for proper development of olfactory and most peripheral autonomic neurons, and for the formation of distinct neuronal circuits within the central nervous system. We have previousl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3090

    authors： Persson P,Jögi A,Grynfeld A,Påhlman S,Axelson H

    更新日期：2000-07-21 00:00:00

  • SECReT of the eukaryotic large ribosomal subunit.

    abstract：:The large ribosomal subunit of the thermophilic fungus Thermomyces lanuginosus was reconstructed in three dimensions from electron micrographs. This is the first reported reconstruction of the eukaryotic complex and demonstrates specific structural features such as an intersubunit canyon and a potential channel for th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1263

    authors： Harauz G,Zuzan H,Kim PT,Holbrook JA

    更新日期：1995-02-15 00:00:00

  • Apoaequorin monitors degradation of endoplasmic reticulum (ER) proteins initiated by loss of ER Ca(2+).

    abstract：:Apoaequorin was targeted to the cytosol, nucleus, and endoplasmic reticulum of HeLa cells in order to determine the effect of Ca(2+) release from the ER on protein degradation. In resting cells apoaequorin had a rapid half-life (ca. 20-30 min) in the cytosol or nucleus, but was relatively stable for up to 24 h in the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2194

    authors： Jeffery J,Kendall JM,Campbell AK

    更新日期：2000-02-24 00:00:00

  • Stable interdomain interaction within the cytoplasmic domain of CD45 increases enzyme stability.

    abstract：:CD45 is a leukocyte-specific, two domain transmembrane tyrosine phosphatase. Co-purification of a recombinant protein containing the first phosphatase domain of CD45 (6His-D1) with a recombinant protein containing the second phosphatase domain (GST-D2) from E. coli indicated a stable interaction which resulted in incr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2623

    authors： Felberg J,Johnson P

    更新日期：2000-05-10 00:00:00

  • Growth differentiation factor-15 induces expression of ATP-binding cassette transporter A1 through PI3-K/PKCζ/SP1 pathway in THP-1 macrophages.

    abstract：OBJECTIVE:The aim of this study was to determine whether ATP-binding cassette transporter A1 (ABCA1) was up-regulated by growth differentiation factor-15 (GDF-15) via the phosphoinositide 3-kinase (PI3K)/protein kinase Cζ (PKCζ)/specificity protein 1 (SP1) pathway in THP-1 macrophages. METHODS AND RESULTS:We investiga...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.01.048

    authors： Wu JF,Wang Y,Zhang M,Tang YY,Wang B,He PP,Lv YC,Ouyang XP,Yao F,Tan YL,Tang SL,Tang DP,Cayabyab FS,Zheng XL,Zhang DW,Zeng GF,Tang CK

    更新日期：2014-02-14 00:00:00

  • The polarity protein Par6 is coupled to the microtubule network during molluscan early embryogenesis.

    abstract：:Cell polarity, which directs the orientation of asymmetric cell division and segregation of fate determinants, is a fundamental feature of development and differentiation. Regulators of polarity have been extensively studied, and the critical importance of the Par (partitioning-defective) complex as the polarity machi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.11.087

    authors： Homma T,Shimizu M,Kuroda R

    更新日期：2011-01-07 00:00:00

  • Requirement of SIRPα for protective immunity against Leishmania major.

    abstract：:Signal regulatory protein α (SIRPα) is a transmembrane protein that binds the protein tyrosine phosphatases SHP-1 and SHP-2 through its cytoplasmic region and is abundantly expressed on dendritic cells and macrophages. Wild-type (WT) C57BL/6 mice are known to be resistant to Leishmania major infection. We here found t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.09.062

    authors： Morimoto N,Murata Y,Motegi S,Suzue K,Saito Y,Okazawa H,Ohnishi H,Kotani T,Kusakari S,Ishikawa O,Matozaki T

    更新日期：2010-10-22 00:00:00

  • Exogastrula formation in Xenopus laevis embryos depleted with maternal XmN-cadherin mRNA by antisense S-oligo DNA.

    abstract：:Xenopus XmN-cadherin gene appears to have dual functions, since its mRNA is maternally provided in unfertilized eggs, once disappears almost completely during gastrula stage, then accumulates again specifically in neural tissues in later stage embryos. In the present experiment, we first followed the change in XmN-cad...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7490

    authors： Hojyo T,Tooi O,Tashiro K,Shiokawa K

    更新日期：1998-01-06 00:00:00

  • Supercomplex formation between Mlh1-Mlh3 and Sgs1-Top3 heterocomplexes in meiotic yeast cells.

    abstract：:The genome of Saccharomyces cerevisia encodes four mismatch repair MutL proteins and these proteins form three heterocomplexes: Mlh1-Mlh2, Mlh1-Mlh3, and Mlh1-Pms1. Only, the Mlh1-Mlh3 heterocomplex has been implicated specifically in promotion of meiotic crossing-over. In this report, we show that yeast Mlh3 co-immun...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02034-x

    authors： Wang TF,Kung WM

    更新日期：2002-08-30 00:00:00