Gangliosides expressed on breast cancer cells are E-selectin ligands.


:Cancer cell adhesion to vascular endothelium is a critical process in hematogenous metastasis. We hypothesized that breast cancer cells express ligands that bind under blood flow conditions to E-selectin expressed by endothelial cells. At a hemodynamic wall shear rate, BT-20 and MDA-MB-468 breast cancer cells adhered to cytokine-activated human umbilical cord vein endothelial cells (HUVECs) but not to anti-E-selectin monoclonal antibody treated HUVECs, demonstrating that adhesion was specifically mediated by E-selectin. Characterization of glycans expressed on breast cancer cells by a panel of antibodies revealed that BT-20 cells expressed sialyl Lewis X (sLe(x)) and sialyl Lewis A (sLe(a)) but MDA-MB-468 cells did not, suggesting that the former possess classical glycans involved in E-selectin mediated adhesion while the latter have novel binding epitopes. Protease treatment of the breast cancer cells failed to significantly alter the carbohydrate expression profiles, binding to soluble E-selectin-Ig chimera, or the ability of the cells to tether and roll on E-selectin expressed by HUVECs, indicating that glycosphingolipids are functional E-selectin ligands on these cells. Furthermore, extracted breast cancer cell gangliosides supported binding of E-selectin-Ig chimera and adhesion of E-selectin transfected cells under physiological flow conditions. In summary, our results demonstrate that breast cancer cells express sialylated glycosphingolipids (gangliosides) as E-selectin ligands that may be targeted for prevention of metastasis.


Shirure VS,Henson KA,Schnaar RL,Nimrichter L,Burdick MM




Has Abstract


2011-03-18 00:00:00














  • Ligand binding to the human MT2 melatonin receptor: the role of residues in transmembrane domains 3, 6, and 7.

    abstract::To better understand the mechanism of interactions between G-protein-coupled melatonin receptors and their ligands, our previously reported homology model of human MT2 receptor with docked 2-iodomelatonin was further refined and used to select residues within TM3, TM6, and TM7 potentially important for receptor-ligand...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mazna P,Berka K,Jelinkova I,Balik A,Svoboda P,Obsilova V,Obsil T,Teisinger J

    更新日期:2005-07-08 00:00:00

  • Computational studies of the binding mechanism of calmodulin with chrysin.

    abstract::Calmodulin (CaM) plays a crucial role in metabolism and physiology of eukaryotes by regulating biological activities. Multiple lines of evidences indicate that the phosphorylated flavonoids possess relatively stronger affinities for proteins by forming non-covalent complexes with them, and that the cellular functions ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Li L,Wei DQ,Wang JF,Chou KC

    更新日期:2007-07-13 00:00:00

  • Autocatalytic processing of procathepsin E to cathepsin E and their structural differences.

    abstract::The processing of human gastric procathepsin E to its mature form, cathepsin E, was studied at pH 3.5. The results revealed the autocatalytic and apparently one-step conversion of procathepsin E to cathepsin E within 10 min of incubation at 14 degrees C under the conditions used. Analyses of the amino acid sequences o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Athauda SB,Takahashi T,Kageyama T,Takahashi K

    更新日期:1991-02-28 00:00:00

  • Differential subcellular localisation of two isoforms of p70 S6 protein kinase.

    abstract::Ribosomal protein S6 phosphorylation is stimulated by a variety of hormones and growth factors. In mammalian cells the predominant S6 kinase is a 70 kDa enzyme which exists in two forms differing only in their amino terminal region. To determine whether this difference is functionally significant we have examined the ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Coffer PJ,Woodgett JR

    更新日期:1994-01-28 00:00:00

  • Dimerization of human recombinant resistin involves covalent and noncovalent interactions.

    abstract::Resistin, an adipocyte secreted cysteine rich hormone has been implicated as molecular link between obesity and type 2 diabetes in a murine model. Although, at the protein level mouse and human resistin show remarkable similarities with respect to conserved cysteine residues, the physiological role of human resistin i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Raghu P,Ghosh S,Soundarya K,Haseeb A,Aruna B,Ehtesham NZ

    更新日期:2004-01-16 00:00:00

  • Regulation of tight junction permeability by sodium caprate in human keratinocytes and reconstructed epidermis.

    abstract::Tight junctions (TJs) restrict paracellular flux of water and solutes in epithelia and endothelia. In epidermis, the physiological role of TJs is not fully understood. In this study, sodium caprate (C10), which dilates intestinal TJs, was applied to cultured human epidermal keratinocytes and reconstructed human epider...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kurasawa M,Kuroda S,Kida N,Murata M,Oba A,Yamamoto T,Sasaki H

    更新日期:2009-04-03 00:00:00

  • Molecular cloning of anti-SS-A/Ro 60-kDa peptide Fab fragments from infiltrating salivary gland lymphocytes of a patient with Sjögren's syndrome.

    abstract::Anti-SS-A/Ro antibodies are commonly found in systemic autoimmune diseases such as Sjögren's syndrome and systemic lupus erythematosus, and some of these antibodies appear to be responsible for certain pathological lesions including congenital heart block in neonatal lupus. In this study, we generated three human anti...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Suzuki H,Takemura H,Suzuki M,Sekine Y,Kashiwagi H

    更新日期:1997-03-06 00:00:00

  • ATP enhances at low concentrations but dissolves at high concentrations liquid-liquid phase separation (LLPS) of ALS/FTD-causing FUS.

    abstract::ATP is the universal energy currency but mysteriously its cellular concentration is much higher than that needed for providing energy. Recently ATP was decoded to act as a hydrotrope to dissolve liquid-liquid phase separation (LLPS) of FUS whose aggregation leads to ALS/FTD. By DIC microscopy and NMR, here we characte...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kang J,Lim L,Song J

    更新日期:2018-10-02 00:00:00

  • Transforming growth factor-beta1 stimulated protein kinase B serine-473 and focal adhesion kinase tyrosine phosphorylation dependent on cell adhesion in human hepatocellular carcinoma SMMC-7721 cells.

    abstract::Transforming growth factor-beta1 (TGF-beta1) is a potent growth inhibitor and apoptosis inducer for most normal cells. However, tumor cells are commonly nevertheless sensitive to the tumor-suppressing effects of TGF-beta1. In this paper, we focus on the effects of TGF-beta1 on two important anti-apoptotic protein kina...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Xu Z,Ma DZ,Wang LY,Su JM,Zha XL

    更新日期:2003-12-12 00:00:00

  • Evolutionary conservation of transferrin genomic organization and expression characterization in seven freshwater turtles.

    abstract::Serum transferrin (tf), encoding an iron-binding glycoprotein, has been revealed to play important roles in iron transportation and immune response, and it also has been demonstrated to be valuable for phylogenetic analysis in vertebrates. However, the evolutionary conservation, expression profiles and positive select...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Liu XL,Wang YK,Ouyang S,Zhu YY,Li W,Hong XY,Xu HY,Zhu XP

    更新日期:2018-12-02 00:00:00

  • Nitric oxide contributes to tissue injury in mercuric chloride-induced autoimmunity.

    abstract::Recent data has suggested a role for nitric oxide (NO) both in the induction of immunity and as an effector of tissue injury in experimental models of inflammation. In this study, we have tested the efficacy of two inhibitors of NO synthase, NG-monomethyl-L-arginine (L-NMMA) and aminoguanidine (AG), to modify the auto...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Woolfson RG,Qasim FJ,Thiru S,Oliveira DB,Neild GH,Mathieson PW

    更新日期:1995-12-14 00:00:00

  • BAP1 regulates AMPK-mTOR signalling pathway through deubiquitinating and stabilizing tumour-suppressor LKB1.

    abstract::Liver kinase B1 (LKB1), a tumour suppressor, participates in many cellular processes, including cell survival, growth, apoptosis, transformation, and metabolism. Upon performing yeast two-hybrid screening, co-immunoprecipitation, and GST pull-down, we identified that BRCA1-associated protein 1 (BAP1), a deubiquitinase...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yang C,Ding H,Yang Y,Yang L,Yang Y,Fang M,Ren J,Hu R,Wang C,Geng W

    更新日期:2020-09-03 00:00:00

  • Effects of SH-group reagents on Ca2+ and K+ channel currents of pancreatic B-cells.

    abstract::The whole-cell patch-clamp technique has been applied to elucidate the effects of H2O2 and the SH-group reagent 2,2'-dithio-bis(5-nitropyridine) (DTBNP) on Ca2+ currents of mouse pancreatic B-cells. We also tested the effect of DTBNP on the whole-cell K+ ATP current. DTBNP (20 and 100 mumol/l) blocked both the current...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Krippeit-Drews P,Britsch S,Lang F,Drews G

    更新日期:1994-04-29 00:00:00

  • Negatively charged residues (Asp378 and Asp379) in the last ten amino acids of the C-terminal tail of Cx43 hemichannels are essential for loop/tail interactions.

    abstract::Connexin 43 (Cx43)-hemichannel activity is controlled by intramolecular interactions between cytoplasmic loop and C-terminal tail. We previously identified the last 10 amino acids of the C-terminal tail of Cx43 as essential for Cx43-hemichannel activity. We developed a cell-permeable peptide covering this sequence (TA...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: D'hondt C,Iyyathurai J,Wang N,Gourdie RG,Himpens B,Leybaert L,Bultynck G

    更新日期:2013-03-22 00:00:00

  • Morquio disease: isolation, characterization and expression of full-length cDNA for human N-acetylgalactosamine-6-sulfate sulfatase.

    abstract::We cloned and sequenced a full-length cDNA of human placental N-acetylgalactosamine-6-sulfate sulfatase, the enzyme deficient in Morquio disease. The 2339-nucleotide sequence contained 1566 nucleotides which encoded a polypeptide of 522 amino acid residues. The deduced amino acid sequence was composed of a 26-amino ac...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tomatsu S,Fukuda S,Masue M,Sukegawa K,Fukao T,Yamagishi A,Hori T,Iwata H,Ogawa T,Nakashima Y

    更新日期:1991-12-16 00:00:00

  • A Prdm8 target gene Ebf3 regulates multipolar-to-bipolar transition in migrating neocortical cells.

    abstract::Precise control of neuronal migration is essential for the development of the neocortex. However, the molecular mechanisms underlying neuronal migration remain largely unknown. Here we identified helix-loop-helix transcription factor Ebf3 as a Prdm8 target gene, and found that Ebf3 is a key regulator of neuronal migra...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Iwai R,Tabata H,Inoue M,Nomura KI,Okamoto T,Ichihashi M,Nagata KI,Mizutani KI

    更新日期:2018-01-01 00:00:00

  • Biochemical studies on proacrosin and acrosin from epididymal boar spermatozoa: in vitro translation of boar testicular proacrosin mRNA.

    abstract::An inactive form of acrosin was extracted from epididymal boar spermatozoa utilizing acid pH conditions. When subjected to activation in alkaline environment, this form turns into an enzymatically active species, which exhibits close-related electrophoretic characteristics. Both the precursor and the activated species...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Berruti G,Merigioli G,Martegani E

    更新日期:1986-07-16 00:00:00

  • Corticotropin-releasing factor differentially regulates proopiomelanocortin messenger ribonucleic acid levels in anterior as compared to intermediate pituitary lobes of rats.

    abstract::Chronic subcutaneous infusion of small doses (0.1 microgram/h) of ovine corticotropin-releasing factor (oCRF) into rats for 8 days resulted in differential alteration of proopiomelanocortin (POMC) mRNA levels in the individual pituitary lobes: In the anterior lobe POMC mRNA levels, quantitated by hybridisation using a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Höllt V,Haarmann I

    更新日期:1984-10-30 00:00:00

  • Selective enhancement of the induction of alpha-lactalbumin activity in rat mammary explants by epidermal growth factor.

    abstract::Epidermal growth factor (EGF) enhances the induction of alpha-lactalbumin in mammary explants from pregnant and virgin rats in the presence of insulin (I), hydrocortisone (F) and prolactin (P). EGF also enhances the prolactin-independent induction of alpha-lactalbumin in tissue from pregnant rats and evokes prolactin-...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sankaran L,Topper YJ

    更新日期:1983-12-16 00:00:00

  • Specificity of thyroglobulin interactions with thyroid cells and membranes.

    abstract::Homologous species specificity is demonstrated with bovine and human thyroglobulin in which the two terminal sugars of the B carbohydrate chain, sialic acid and galactose have been removed by enzymatic hydrolysis. The species specificity is demonstrated by measuring the ability of the deglycosylated thyroglobulin deri...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rotella CM,Tanini A,Consiglio E,Shifrin S,De Luca M,Toccafondi R,Kohn LD

    更新日期:1983-08-12 00:00:00

  • Pro-remodeling peptides modulate collagen α1(I) promoter activity in rat cardiac myofibroblasts.

    abstract::Previous studies have extensively demonstrated the effect of endothelin-1 (ET-1), angiotensin II (Ang II), and TGF-β1 on the stimulation of collagen type I expression in cardiac myofibroblasts. However, the role of pro-remodeling peptides in the transcriptional regulation of the collagen promoter remains unclear. Thus...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kisling A,Katwa LC

    更新日期:2019-08-06 00:00:00

  • Inhibin and beta type transforming growth factor (TGF beta) have opposite modulating effects on the follicle stimulating hormone (FSH)-induced aromatase activity of cultured rat granulosa cells.

    abstract::We have recently observed that attomolar concentration of exogenously added TGF beta, a molecule structurally related to inhibin, can stimulate the basal secretion of FSH in a pituitary cell culture. Inhibin purified from porcine follicular fluid antagonizes this activity of TGF beta. To understand further the homeost...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ying SY,Becker A,Ling N,Ueno N,Guillemin R

    更新日期:1986-05-14 00:00:00

  • Nrf2 regulates cellular behaviors and Notch signaling in oral squamous cell carcinoma cells.

    abstract::Oxidative stress is known to play a pivotal role in the development of oral squamous cell carcinoma (OSCC). We have demonstrated that activation of the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway has chemopreventive effects against oxidative stress-associated OSCC. However, Nrf2 have dual role...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Fan H,Paiboonrungruan C,Zhang X,Prigge JR,Schmidt EE,Sun Z,Chen X

    更新日期:2017-11-04 00:00:00

  • [8]-Gingerol inhibits melanogenesis in murine melanoma cells through down-regulation of the MAPK and PKA signal pathways.

    abstract::[8]-Gingerol is an active component of Zinger and shows several pharmacological activities, such as antipyretic and anti-inflammation characteristics. To identify a potential skin-whitening agent, the inhibitory effects of [8]-gingerol on melanogenesis and its mechanism of action were investigated. In the present stud...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Huang HC,Chou YC,Wu CY,Chang TM

    更新日期:2013-08-23 00:00:00

  • Human receptor for activated protein kinase C1 associates with polyglutamine aggregates and modulates polyglutamine toxicity.

    abstract::Formation of SDS-insoluble protein aggregates in affected neurons is a cellular pathological feature of polyglutamine (polyQ) disease. We identified a multi-WD-domain protein, receptor for activated protein kinase C1 (RACK1), as a novel polyQ aggregate component from a Drosophila transgenic polyQ disease model. We sho...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lam W,Chan WM,Lo TW,Wong AKY,Wu CC,Chan HYE

    更新日期:2008-12-12 00:00:00

  • Identification of an interleukin-6 responsive element and characterization of the proximal promoter region of the rat hemopexin gene.

    abstract::The rat hemopexin (Hx) gene was isolated and studies of its transcriptional regulation initiated. For analysis by a transient expression assay, the sequence between -2400 and +21 and sequential 5' truncates were linked to the chloramphenicol acetyltransferase (CAT) gene. HepG2 cells transfected with these CAT construc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nagae Y,Muller-Eberhard U

    更新日期:1992-05-29 00:00:00

  • A single replacement of histidine to arginine in EGFR-lytic hybrid peptide demonstrates the improved anticancer activity.

    abstract::We previously reported that novel targeted "hybrid peptide" in which epidermal growth factor receptor (EGFR) binding peptide was conjugated with lytic-type peptide had selective cytotoxic activity to EGFR expressing cancer cell lines, and in vivo analysis revealed that this EGFR-lytic peptide displayed significant ant...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tada N,Horibe T,Haramoto M,Ohara K,Kohno M,Kawakami K

    更新日期:2011-04-08 00:00:00

  • Maternal circulating syncytiotrophoblast-derived extracellular vesicles contain biologically active 5'-tRNA halves.

    abstract::The placenta releases syncytiotrophoblast-derived extracellular vesicles (STB-EV) into the maternal circulation throughout gestation. STB-EV dependent signalling is believed to contribute to the widespread maternal adaptive physiological changes seen in pregnancy. Transfer RNA (tRNA) halves have been identified in ves...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Cooke WR,Cribbs A,Zhang W,Kandzija N,Motta-Mejia C,Dombi E,Ri R,Cerdeira AS,Redman C,Vatish M

    更新日期:2019-10-08 00:00:00

  • Different proliferative responses of periportal and perivenous hepatocytes to EGF.

    abstract::Stimulation of DNA synthesis by EGF was compared in cultured periportal and perivenous hepatocyte populations. Periportal hepatocytes responded to EGF more sensitive (IC50-values 20 vs 75 ng/ml) and with a higher maximal stimulation (420 vs 290%) than perivenous hepatocytes with respect to both [3H]thymidine incorpora...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Gebhardt R,Jonitza D

    更新日期:1991-12-31 00:00:00

  • Endothelin stimulates accumulations of cellular atrial natriuretic peptide and its messenger RNA in rat cardiocytes.

    abstract::The effect of endothelin-1 (ET-1) on secretion and synthesis of rat atrial natriuretic peptide (rANP) as well as its mRNA levels was studied in primary cultures of neonatal rat atrial cardiocytes. ET-1 dose-dependently (10(-10)-10(-7) M) increased media and cellular rANP-like immunoreactivity as well as its cytoplasmi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Fukuda Y,Hirata Y,Taketani S,Kojima T,Oikawa S,Nakazato H,Kobayashi Y

    更新日期:1989-11-15 00:00:00