Gangliosides expressed on breast cancer cells are E-selectin ligands.


:Cancer cell adhesion to vascular endothelium is a critical process in hematogenous metastasis. We hypothesized that breast cancer cells express ligands that bind under blood flow conditions to E-selectin expressed by endothelial cells. At a hemodynamic wall shear rate, BT-20 and MDA-MB-468 breast cancer cells adhered to cytokine-activated human umbilical cord vein endothelial cells (HUVECs) but not to anti-E-selectin monoclonal antibody treated HUVECs, demonstrating that adhesion was specifically mediated by E-selectin. Characterization of glycans expressed on breast cancer cells by a panel of antibodies revealed that BT-20 cells expressed sialyl Lewis X (sLe(x)) and sialyl Lewis A (sLe(a)) but MDA-MB-468 cells did not, suggesting that the former possess classical glycans involved in E-selectin mediated adhesion while the latter have novel binding epitopes. Protease treatment of the breast cancer cells failed to significantly alter the carbohydrate expression profiles, binding to soluble E-selectin-Ig chimera, or the ability of the cells to tether and roll on E-selectin expressed by HUVECs, indicating that glycosphingolipids are functional E-selectin ligands on these cells. Furthermore, extracted breast cancer cell gangliosides supported binding of E-selectin-Ig chimera and adhesion of E-selectin transfected cells under physiological flow conditions. In summary, our results demonstrate that breast cancer cells express sialylated glycosphingolipids (gangliosides) as E-selectin ligands that may be targeted for prevention of metastasis.


Shirure VS,Henson KA,Schnaar RL,Nimrichter L,Burdick MM




Has Abstract


2011-03-18 00:00:00














  • Human cartilage from late stage familial osteoarthritis transcribes type II collagen mRNA encoding a cysteine in position 519.

    abstract::A single base change resulting in the substitution of Cys for Arg at position 519 of the type II collagen triple helix is a predisposing factor in the pathogenesis of a precocious-onset form of familial osteoarthritis associated with a mild chondrodysplasia. Cartilage obtained at the time of total knee replacement in ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Holderbaum D,Malemud CJ,Moskowitz RW,Haqqi TM

    更新日期:1993-05-14 00:00:00

  • Characterization of novel inhibitors of cyclin-dependent kinases.

    abstract::In epithelial cells progression through the G1 phase of the cell cycle and preparing the cell for the S phase is regulated by cyclin D1-cdk4. Cells that express the retinoblastoma protein (pRb) are dependent on cyclin D1-cdk4 activity for their proliferation while cells that do not express pRb are not. Overexpression ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kent LL,Hull-Campbell NE,Lau T,Wu JC,Thompson SA,Nori M

    更新日期:1999-07-14 00:00:00

  • Mitochondrial tRNASer(UCN) gene is the hot spot for mutations associated with aminoglycoside-induced and non-syndromic hearing loss.

    abstract::Mutations in mitochondrial DNA is one of the important causes of hearing loss. Here, we performed a mutational screening of tRNA(Ser(UCN)) gene in 1542 Chinese subjects with hearing loss. One subject and five subjects carried tRNA(Ser(UCN)) A7445C and G7444A mutations, respectively, while two subjects harbored both G7...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Jin L,Yang A,Zhu Y,Zhao J,Wang X,Yang L,Sun D,Tao Z,Tsushima A,Wu G,Xu L,Chen C,Yi B,Cai J,Tang X,Wang J,Li D,Yuan Q,Liao Z,Chen J,Li Z,Lu J,Guan MX

    更新日期:2007-09-14 00:00:00

  • Multiple processing of procathepsin L to cathepsin L in vivo.

    abstract::Three amino-terminal-specific peptidic antibodies against cathepsin L were generated. These antibodies recognize in vitro processing products of procathepsin L in time-course-dependent fashion. Immunoblot analyses with these antibodies followed by immunoprecipitation with anti-cathepsin L antibody showed that the amin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ishidoh K,Saido TC,Kawashima S,Hirose M,Watanabe S,Sato N,Kominami E

    更新日期:1998-11-09 00:00:00

  • Homeodomain protein CDX2 regulates COX-2 expression in colorectal cancer.

    abstract::CDX2 is an intestine-specific tumor suppressor gene encoding homeodomain-containing transcription factor, which is involved in a variety of developmental, proliferating, and differentiating processes. Moreover, the expression of CDX2 is reduced in a subset of primary colorectal cancers. In contrast, cyclooxygenase-2 (...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kim SP,Park JW,Lee SH,Lim JH,Jang BC,Lee SH,Jang IH,Freund JN,Suh SI,Mun KC,Song DK,Ha EM,Lee WJ,Kwon TK

    更新日期:2004-02-27 00:00:00

  • Hormonal effects on the development changes of mouse small intestinal glycolipids.

    abstract::The composition of intestinal glycosphingolipids during normal and hormone-perturbed development was investigated. The concentrations of glycosphingolipids of mouse small intestine were affected by the injection of thyroxine or cortisone during suckling and weaning periods. GDla was reduced by the hormonal treatment a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sato E,Fujie M,Uezato T,Fujita M,Nishimura K

    更新日期:1984-03-30 00:00:00

  • Transient activation of c-myc protooncogene expression in Leydig cells by human chorionic gonadotropin.

    abstract::This study evaluated the effects of in vivo administration of human chorionic gonadotropin (hCG) on c-myc oncogene expression in Leydig cells. Sprague-Dawley rats (46-50 days old) were treated with hCG (10 units, i.p.), and purified Leydig cells were isolated 1-24 h later. HCG caused a transient elevation of c-myc mRN...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lin T,Blaisdell J,Barbour KW,Thompson EA

    更新日期:1988-11-30 00:00:00

  • Reversal of P-glycoprotein-mediated multidrug resistance by 5,6,7,3',4'-pentamethoxyflavone (Sinensetin).

    abstract::Multidrug resistance (MDR) cells can be sensitized to anticancer drugs when treated concomitantly with chemosensitizers. In this study, chemosensitizing effects of 5,6,7,3',4'-pentamethoxyflavone (sinensetin) and its analogs were investigated with respect to in vitro efficacy and structure-activity relationship. Sinen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Choi CH,Sun KH,An CS,Yoo JC,Hahm KS,Lee IH,Sohng JK,Kim YC

    更新日期:2002-07-26 00:00:00

  • Function of the PLZF gene in early development and self-renewal of T cells in mice.

    abstract:BACKGROUND/AIMS:The expression of transcription factor Zbtb1 is essential for the maintenance and development of various blood cells in the hematopoietic system. In the current study, we found that the total number of thymocytes in PLZF deficient mice was reduced compared with thymocytes in wild-type mice, and the numb...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Du JL,Cao X,Liu HY,Zeng Y,Yang XC,Wan XM,Chang FF,Zhao TY,Jia XY,Wang HZ,Liu J,Cai KZ,Ma ZR

    更新日期:2019-04-16 00:00:00

  • Preparation and partial characterization of polyclonal antibodies raised against a preselected sequence of calcium dependent lectins.

    abstract::The synthetic-peptide strategy was used to generate antibodies raised against calcium-dependent lectins of vertebrates. We demonstrate that a synthetic peptide predicted from the amino acid sequence of the carbohydrate recognition domain can induce blocking antibodies which would react with, or in close vicinity of, t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Blanck O,Thibault V,Granier C,Van Rietschoten J,Courageot J,Miquelis R

    更新日期:1990-06-29 00:00:00

  • MicroRNA-140 suppresses osteosarcoma tumor growth by enhancing anti-tumor immune response and blocking mTOR signaling.

    abstract::Immune checkpoint blockade is proved to be a promising therapeutic strategy against several human malignancies. MicroRNAs (miRNAs) are ∼22 nucleotide RNAs that play important roles in regulating gene expression, either suppressing its translation or speeding up its degradation. In this study, we demonstrated that miR-...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ji X,Wang E,Tian F

    更新日期:2018-01-01 00:00:00

  • Identification and gene structure of a novel human PLZF-related transcription factor gene, TZFP.

    abstract::A novel cDNA clone was identified through yeast two-hybrid experiments. Following cross-examination between the cDNA clones, EST clones, and the cosmid clone, we could digitally assemble a new zinc finger transcription factor gene. This predicted gene has a cDNA size of about 1960 bp and is translated into a 487-amino...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lin W,Lai CH,Tang CJ,Huang CJ,Tang TK

    更新日期:1999-11-02 00:00:00

  • Hyperoxia-induced ciliary loss and oxidative damage in an in vitro bovine model: the protective role of antioxidant vitamins E and C.

    abstract::Although elevated oxygen fraction is used in intensive care units around the world, pathological changes in pulmonary tissue have been shown to occur with prolonged exposure to hyperoxia. In this work a bovine bronchus culture model has been successfully used to evaluate the effects of hyperoxia on ciliated epithelium...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Al-Shmgani HS,Moate RM,Sneyd JR,Macnaughton PD,Moody AJ

    更新日期:2012-12-14 00:00:00

  • Cytochrome P450 induction by phenobarbital (PB) is inhibited by 12-O-tetradecanoylphorbol-13-acetate (TPA): evidence that protein kinase C regulates induction.

    abstract::The hepatic microsomal monooxygenase system was studied in hypophysectomized male rats exposed for 24 or 48 h to PB and/or TPA, an activator of kinase C. TPA attenuated basal and PB-induced levels of P450, aniline hydroxylase (ANH), ethylmorphine demethylase (EDM) and cytochrome c reductase. Hence, PB may effect induc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Steele DF,Virgo BB

    更新日期:1988-06-16 00:00:00

  • VEGFR-2-specific ligand VEGF-E induces non-edematous hyper-vascularization in mice.

    abstract::VEGF family members play important roles in angiogenesis and vascular permeability. VEGF-A-transgenic mice showed an increased vascularization with edema due to hyper-vascular permeability and subcutaneous hemorrhage as side effects. VEGF-A binds and activates two receptors, VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). To...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kiba A,Sagara H,Hara T,Shibuya M

    更新日期:2003-02-07 00:00:00

  • Promotion of lipogenesis by PPARγ-activated FXR expression in adipocytes.

    abstract::Farnesoid X receptor (FXR), a bile acid receptor, is known to be involved in the promotion of adipogenesis. However, the regulation mechanism of FXR-promoted adipogenesis remains unclear. In this study, we investigated the regulation mechanism of FXR-mediated activation of adipogenesis in murine adipocyte 3T3-L1 cells...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Shinohara S,Fujimori K

    更新日期:2020-06-18 00:00:00

  • Characterization of calcineurin-dependent response element binding protein and its involvement in copper-metallothionein gene expression in Neurospora.

    abstract::In continuation of our recent observations indicating the presence of a lone calcineurin-dependent response element (CDRE) in the -3730bp upstream region of copper-induced metallothionein (CuMT) gene of Neurospora [K.S. Kumar, S. Dayananda, C. Subramanyam, Copper alone, but not oxidative stress, induces copper-metallo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kumar KS,Ravi Kumar B,Siddavattam D,Subramanyam C

    更新日期:2006-07-07 00:00:00

  • Isozyme analysis of human normal polymorphonuclear leukocyte phosphofructokinase.

    abstract::Phosphofructokinase (PFK) from human polymorphonuclear leukocytes (PMN) was characterized by immunological titration with subunit specific antibodies, column chromatography on QAE-Sephadex and SDS-polyacrylamide gel electrophoresis. Two different isozymes, M-type and L-type, were found. The M(r) values of the M and L ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Durante P,Raleigh X,Gómez ME,Campos G,Ryder E

    更新日期:1995-11-22 00:00:00

  • The tea flavonoid epigallocatechin-3-gallate reduces cytokine-induced VCAM-1 expression and monocyte adhesion to endothelial cells.

    abstract::Attachment of leukocytes to the vascular endothelium and the subsequent migration of cells into the vessel wall are early events in atherogenesis. This process requires the expression of endothelial adhesion molecules. Since tea catechins are reputed to promote antiatherogenic activities, we investigated the effects o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ludwig A,Lorenz M,Grimbo N,Steinle F,Meiners S,Bartsch C,Stangl K,Baumann G,Stangl V

    更新日期:2004-04-09 00:00:00

  • Atorvastatin augments temozolomide's efficacy in glioblastoma via prenylation-dependent inhibition of Ras signaling.

    abstract::Ras signaling is often dysregulated and plays essential roles for the maintenance of glioblastoma. The proper function of Ras depends largely on the appropriate post-translational modification termed prenylation. Targeting protein prenylation therefore represents an alternative therapeutic strategy in glioblastoma. In...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Peng P,Wei W,Long C,Li J

    更新日期:2017-07-29 00:00:00

  • Nickel(II) acetate-treated Chinese hamster ovary cells differentially express Vimentin, hSNF2H homologue, and H ferritin.

    abstract::In probing the possible non-genotoxic molecular mechanism(s) of nickel(II)-induced carcinogenesis, we performed a non-radioactive mRNA differential display analysis for nickel(II) acetate-treated Chinese hamster ovary cells (CHO-K1-BH4). Three out of thirty differentially expressed cDNAs had sequences highly similar t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lee SH,Shiao YH,Plisov SY,Kasprzak KS

    更新日期:1999-05-19 00:00:00

  • Stimulatory effect of endogenous tissue inhibitor of metalloproteinases-1 (TIMP-1) overexpression on type IV collagen and laminin gene expression in rat mammary carcinoma cells.

    abstract::We recently reported enhanced tumor growth and stimulation of vascular endothelial growth factor (VEGF) expression in rat mammary carcinoma cells transfected with a human tissue inhibitor of metalloproteinases-1 (hTIMP-1) cDNA (1). In the present study, we examined if the composition of the stroma was altered in the t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yoshiji H,Buck TB,Harris SR,Ritter LM,Lindsay CK,Thorgeirsson UP

    更新日期:1998-06-29 00:00:00

  • UBE2S enhances the ubiquitination of p53 and exerts oncogenic activities in hepatocellular carcinoma.

    abstract::Ubiquitin-conjugating enzyme E2S (UBE2S) plays pivotal roles in the progression of human cancers. However, its clinical significance and role in hepatocellular carcinoma (HCC) remain unknown. Here, we show that UBE2S is upregulated in HCC and exhibits oncogenic activities via enhancing the ubiquitination of p53. Incre...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pan YH,Yang M,Liu LP,Wu DC,Li MY,Su SG

    更新日期:2018-09-05 00:00:00

  • Wheat germ phosphoglycerate mutase: evidence for a metalloenzyme.

    abstract::Wheat germ phosphoglycerate mutase, exposed to 3.4 M guanidinium chloride at 22 degrees C and pH 7.8, slowly undergoes time-dependent inactivation which can be fully reversed by adding excess Co2+ or Mn2+ to a 50-fold dilution of the denaturing medium. Titration of the denatured enzyme with either Co2+ or Mn2+ shows t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Smith GC,McWilliams AD,Hass LF

    更新日期:1986-04-14 00:00:00

  • Mitotic phosphorylation of Aki1 at Ser208 by cyclin B1-Cdk1 complex.

    abstract::Akt kinase-interacting protein 1 (Aki1)/Freud-1/CC2D1A is localized in the cytosol, nucleus, and centrosome. Aki1 plays distinct roles depending on its localization. In the cytosol, it acts as a scaffold protein in the phosphoinositide 3-kinase (PI3K)/3-phosphoinositide-dependent protein kinase 1 (PDK1)/Akt pathway. I...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nakamura A,Naito M,Arai H,Fujita N

    更新日期:2010-03-19 00:00:00

  • Involvement of calcium in the transient depolarization of E.coli cytoplasmic membrane induced by phage adsorption: a study with the fluorescent calcium indicator QUIN2.

    abstract::Adsorption of phage T4 to its outer membrane receptor (the lipopolysaccharide) triggers a transient depolarization of the cytoplasmic membrane which is prevented upon addition of EGTA, suggesting that calcium is necessary for the transmission of the signal between the two membranes. Using the fluorescent indicator QUI...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Boulanger P,Labedan B,Letellier L

    更新日期:1985-09-16 00:00:00

  • New partner proteins containing novel internal recognition motif for human glutaminase interacting protein (hGIP).

    abstract::Regulation of gene expression in cells is mediated by protein-protein, DNA-protein and receptor-ligand interactions. PDZ (PSD-95/Discs-large/ZO-1) domains are protein-protein interaction modules. PDZ-containing proteins function in the organization of multi-protein complexes controlling spatial and temporal fidelity o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zencir S,Banerjee M,Dobson MJ,Ayaydin F,Fodor EA,Topcu Z,Mohanty S

    更新日期:2013-03-01 00:00:00

  • Morphological characterization of adult mouse Leydig cells in culture.

    abstract::The morphology and function of Leydig cells are changed during the development, mature and senility of Leydig cells along the life span of males. This study was to observe the growth morphology of adult mouse Leydig cells in culture, aiming to provide a reference for furthermore understanding of the biological functio...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang JQ,Cao WG

    更新日期:2016-01-22 00:00:00

  • Androgen receptor regulates cardiac fibrosis in mice with experimental autoimmune myocarditis by increasing microRNA-125b expression.

    abstract::Cardiac fibrosis is an important cardiac remodeling event in the development of inflammation dilated cardiomyopathy (iDCM). We have previously observed that degradation enhancer of androgen receptor (ASC-J9®) could improve cardiac inflammation and fibrosis. Using Primary CFs, we demonstrated that ASC-J9® attenuates th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang Y,Ma W,Lu S,Yan L,Hu F,Wang Z,Cheng B

    更新日期:2018-11-17 00:00:00

  • Up-regulation of long noncoding RNA MINCR promotes non-small cell of lung cancer growth by negatively regulating miR-126/SLC7A5 axis.

    abstract::A growing body of evidence suggests that MYC induced long noncoding RNA (MINCR) is involved in the initiation and progression of various tumors. However, little is known about the biological function and clinical value of MINCR in non-small cell lung cancer (NSCLC). In the present study, results found that MINCR over ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang J,Ding M,Zhu H,Cao Y,Zhao W

    更新日期:2019-01-15 00:00:00