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Zyxin is up-regulated during megakaryocytic differentiation of human UT-7/c-mpl cells.

Abstract：

:To characterize genes involved in megakaryocytic commitment, we compared expression profiles of bipotent cells (UT-7/c-mpl) with those of the same cells induced to differentiate towards megakaryopoiesis in the presence of TPO. Using cDNA arrays, we showed that 12 out of 2260 genes changed their expression level after 6h of TPO stimulation. One of these genes encodes for zyxin, a cytoskeleton protein component. Zyxin is up-regulated at the mRNA and protein levels in UT-7/c-mpl cells in response to TPO confirming the reliability of the cDNA array technology. Similarly, when CD34 positive cells were induced to differentiate into megakaryocytes, zyxin mRNA was accumulated. Furthermore, when megakaryocytes were allowed to spread on fibrinogen, formation of stress fibers and lamellipodia was induced and zyxin was localized at the picks of actin stress fibers. These results suggest an important role for zyxin during megakaryocytic differentiation and more precisely in the regulation of the integrin mediated adhesion process in megakaryocytes.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Decraene C,Garçon L,Lacout C,Sabri S,Auffray C,Vainchenker W,Duménil D,Piétu G,Svinartchuk F

doi

10.1016/j.bbrc.2004.04.047

subject

Has Abstract

pub_date

2004-05-28 00:00:00

pages

439-43

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006291X04007545

journal_volume

318

pub_type

杂志文章

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