Monoclonal antibody-directed immunopurification and identification of cytochromes P-450.

abstract：:Accumulating evidence indicates that microRNAs function as oncogenes or tumor suppressor genes in human cancer. MiR-503 is deregulated in various human cancers and has been associated with hepatocellular carcinoma (HCC) progression. However, the underlying mechanisms of miR-503 involvement in the development and progr...

journal_title：Biochemical and biophysical research communications

authors： Li B,Liu L,Li X,Wu L

更新日期：2015-09-04 00:00:00

abstract：:DC-SIGN, a C-type lectin receptor expressed in dendritic cells (DCs), has been identified as a receptor for human immunodeficiency virus type 1, hepatitis C virus, Ebola virus, cytomegalovirus, dengue virus, and the SARS coronavirus. We used H5N1 pseudotyped and reverse-genetics (RG) virus particles to study their abi...

journal_title：Biochemical and biophysical research communications

authors： Wang SF,Huang JC,Lee YM,Liu SJ,Chan YJ,Chau YP,Chong P,Chen YM

更新日期：2008-09-05 00:00:00

abstract：:The delta subunit of the epithelial sodium channel (δENaC) is a member of the ENaC/degenerin family of ion channels. δENaC is distinct from the related α-, β- and γENaC subunits, known for their role in sodium homeostasis and blood pressure control, as δENaC is expressed in brain neurons and activated by external prot...

journal_title：Biochemical and biophysical research communications

authors： Chang T,Ke Y,Ly K,McDonald FJ

更新日期：2011-08-05 00:00:00

abstract：:The human MEP1A gene encodes the meprin alpha subunit that consists of a protease domain conserved in the astacin family of metalloendopeptidases and several C-terminal interaction domains present in other proteins. Using the alpha subunit cDNA, we identified two clones from a human P1-derived artificial chromosome (P...

journal_title：Biochemical and biophysical research communications

authors： Jiang W,Beatty BG

更新日期：1999-07-22 00:00:00

abstract：:Parathymosin has been purified from calf liver and its primary sequence established, except for a segment containing approximately 11 amino acid residues in the central part of the polypeptide chain. Bovine parathymosin contains approximately 101 amino acid residues and shows 90% identity with rat parathymosin, with s...

journal_title：Biochemical and biophysical research communications

authors： Panneerselvam C,Clinton M,Wellner D,Horecker BL

更新日期：1988-09-15 00:00:00

abstract：:Atriopeptin (AP) is expressed in several tissues with each tissue capable of specific differences in processing of the prohormone (pro-AP) to mature low molecular forms of the peptide. Since pro-AP has low biological activity, processing into mature AP is a critical activation event. This observation prompted us to st...

journal_title：Biochemical and biophysical research communications

authors： Shiono S,Suganuma N,Bo M,Boime I,Seibert K,Nakao K,Mukoyama M,Imura H,Needleman P

更新日期：1991-05-15 00:00:00

abstract：:Fecal supernatant or serum containing TT virus (TTV) of genotype 1a (10(5) copies/ml) from patients with acute TTV infection was inoculated intravenously into two naive chimpanzees. Serum samples were obtained weekly and tested for TTV DNA by genotype 1-specific polymerase chain reaction. TTV DNA was detected in chimp...

journal_title：Biochemical and biophysical research communications

authors： Tawara A,Akahane Y,Takahashi M,Nishizawa T,Ishikawa T,Okamoto H

更新日期：2000-11-19 00:00:00

abstract：:Sensitization to Cupressaceae pollen has become one of the most important causes of pollinosis in Western countries during winter and early spring. However, the characterization of the extracts, the allergens involved and the cross-reactivity with other pollen sources still remain poorly studied; in the case of Cupres...

journal_title：Biochemical and biophysical research communications

authors： Pico de Coaña Y,Parody N,Fuertes MÁ,Carnés J,Roncarolo D,Ariano R,Sastre J,Mistrello G,Alonso C

更新日期：2010-10-22 00:00:00

abstract：:The iron molybdenum cofactor of Azotobacter vinelandii nitrogenase has been solubilized for the first time in dimethylformamide and acetonitrile. These solutions have the ability to reconstitute the inactive nitrogenase of the UW 45 mutant of A. vinelandii and exhibit an S = 3/2 EPR signal similar to that for the cofa...

journal_title：Biochemical and biophysical research communications

authors： Lough SM,Jacobs DL,Lyons DM,Watt GD,McDonald JW

更新日期：1986-09-14 00:00:00

abstract：:MDMX is a homolog of MDM2 and is critical for regulating p53 function during mouse development. MDMX level is regulated by MDM2-mediated poly-ubiquitination, which results in its accelerated degradation after DNA damage or expression of ARF. In this report, we demonstrate that MDMX can be modified by conjugation to SU...

journal_title：Biochemical and biophysical research communications

authors： Pan Y,Chen J

更新日期：2005-07-08 00:00:00

abstract：:Isolated rat hepatocytes took up [3H]-aflatoxin B1 during incubation with fifty percent of the aflatoxin B1 covalantly bound to cellular macromolecules. The amount of bound-aflatoxin B1 was proportional to the medium concentration of aflatoxin B1. The specific radioactivity (pmole/mg) of aflatoxin B1 found in the DNA ...

journal_title：Biochemical and biophysical research communications

authors： Ch'ih JJ,Lin T,Devlin TM

更新日期：1983-01-27 00:00:00

abstract：:Thrombin induced tyrosine phosphorylation of 41-kDa and 43-kDa mitogen-activated protein (MAP) kinases in human platelets which was detectable at 1 min and peaked at 2 min after thrombin stimulation as assessed by immunoprecipitation and immunoblotting. The kinase activity, measured by phosphorylation of myelin basic ...

journal_title：Biochemical and biophysical research communications

authors： Nakashima S,Chatani Y,Nakamura M,Miyoshi N,Kohno M,Nozawa Y

更新日期：1994-01-28 00:00:00

abstract：:Oxysterol-binding protein-related proteins are implicated in the sensing and transporting lipids at the membrane contact sites. One of the members of the mammalian ORP family, ORP8, is thought to transport lipids through directly tethering both ER and PM membranes. Targeting to PM is thought to be mediated by N-termin...

journal_title：Biochemical and biophysical research communications

authors： Lee M,Fairn GD

更新日期：2018-02-19 00:00:00

abstract：:Binding of ATP, but not of ADP, activates Escherichia coli DnaA protein for replicational initiation of the chromosome. To elucidate this switching mechanism, we used the affinity-labeling agent ATP-pyridoxal, which forms a covalent bond with the Lys residue located at or near the gamma-phosphate of ATP. ATP-pyridoxal...

journal_title：Biochemical and biophysical research communications

authors： Kubota T,Ito Y,Sekimizu K,Tagaya M,Katayama T

更新日期：2001-11-16 00:00:00

abstract：:The control of cellular adhesion to extracellular matrix proteins is poorly understood. In the present analyses, we set out to test the hypothesis that high galectin-3 concentration on the cell surface downregulates cellular adhesion to the extracellular matrix proteins. Various tumor cell lines were briefly incubated...

journal_title：Biochemical and biophysical research communications

authors： Ochieng J,Leite-Browning ML,Warfield P

更新日期：1998-05-29 00:00:00

abstract：:A number of bioactive peptides are involved in regulating a wide range of animal behaviors, including food consumption. Vertebrate neuropeptide Y (NPY) is a potent stimulator of appetitive behavior. Recently, Drosophila neuropeptide F (dNPF) and short NPF (sNPF), the Drosophila homologs of the vertebrate NPY, were ide...

journal_title：Biochemical and biophysical research communications

authors： Ida T,Takahashi T,Tominaga H,Sato T,Kume K,Ozaki M,Hiraguchi T,Maeda T,Shiotani H,Terajima S,Sano H,Mori K,Yoshida M,Miyazato M,Kato J,Murakami N,Kangawa K,Kojima M

更新日期：2011-07-15 00:00:00

abstract：:Receptor-mediated regulation of guanylate cyclase is well-studied in intact Dictyostelium discoideum cells, but study of the enzyme in cell-free preparations has hampered. A major obstacle has been that in vitro guanylate cyclase activity could be detected only in the presence of unphysiological concentrations of Mn2+...

journal_title：Biochemical and biophysical research communications

authors： Janssens PM,de Jong CC

更新日期：1988-01-15 00:00:00

abstract：:Insulin receptor substrates (IRSs) are phosphorylated by activated insulin/insulin-like growth factor (IGF)-I receptor tyrosine kinases. Phosphotyrosyl IRSs are recognized by signaling molecules possessing src homology region 2 (SH2) domains, which mediate various insulin/IGF bioactivities. However, we have shown that...

journal_title：Biochemical and biophysical research communications

authors： Fukushima T,Arai T,Ariga-Nedachi M,Okajima H,Ooi Y,Iijima Y,Sone M,Cho Y,Ando Y,Kasahara K,Ozoe A,Yoshihara H,Chida K,Okada S,Kopchick JJ,Asano T,Hakuno F,Takahashi S

更新日期：2011-01-21 00:00:00

abstract：:Schisanhenol (Sal) did not diminish the antitumor activity of adriamycin in mice bearing P388 ascites tumor. Sal did not antagonize the suppressive effect of adriamycin on DNA synthesis and cell proliferation in an L1210 ascitic tumor cell culture. Furthermore, Sal at the concentration of 0.1, 0.25, or 1 mM accelerate...

journal_title：Biochemical and biophysical research communications

authors： Lin TJ,Liu GT

更新日期：1991-07-15 00:00:00

abstract：:The induction of human BiP/GRP78 and GAPDH protein genes by the calcium ionophore A23187 was determined. Steady-state levels of mRNA for both the glucose starvation-responsive BiP/GRP78 gene and the glucose-responsive GAPDH gene were dramatically induced in a variety of human cells. There is a homologous palindromatic...

journal_title：Biochemical and biophysical research communications

authors： Chao CC,Yam WC,Lin-Chao S

更新日期：1990-08-31 00:00:00

abstract：:MicroRNAs (miRNAs) have been shown to be involved in the pathophysiological processes of pain. At present, the roles and mechanisms of miRNAs in neonatal repetitive pain are largely unknown. In our research, the expression of miR-140-3p was increased in premature infants who received repetitive painful stimuli since a...

journal_title：Biochemical and biophysical research communications

authors： Zhang J,Yin J,Chen X,Mao X,Xu J,Cheng R,Wu J

更新日期：2019-08-06 00:00:00

abstract：:Interleukin-1 alpha (IL-1alpha) regulates a wide range of important cellular processes. In this study for the first time, we report the cloning, expression, biophysical, and biological characterization of the human interleukin-1alpha. Human IL-1alpha has been expressed in Escherichia coli in high yields ( approximatel...

journal_title：Biochemical and biophysical research communications

authors： Rajalingam D,Kacer D,Prudovsky I,Kumar TK

更新日期：2007-08-31 00:00:00

abstract：:Mouse hepatocytes were found to adhere much stronger to intact native type I collagen than to native, telopeptide-devoid collagen. Removal of telopeptides (by pepsin treatment) caused disintegration of supramolecular collagen aggregates. Similar differences in hepatocyte adhesion were observed between oligomeric and m...

journal_title：Biochemical and biophysical research communications

authors： Berman A,Morozevich G,Karmansky I,Gleiberman A,Bychkova V

更新日期：1993-07-15 00:00:00

abstract：:We measured the amounts of total A beta, A beta 1-40 and A beta 1-42/43 in brain tissues using a newly developed ELISA assay and found that the amounts of insoluble A beta 1-42/43 and insoluble A beta 1-40 were linearly related to the amount of A beta deposits or total insoluble A beta at their lower and higher concen...

journal_title：Biochemical and biophysical research communications

authors： Tamaoka A,Kondo T,Odaka A,Sahara N,Sawamura N,Ozawa K,Suzuki N,Shoji S,Mori H

更新日期：1994-11-30 00:00:00

abstract：:Previous studies suggested that the hydrophobic protein chargerin II, which is encoded in the A6L of mitochondrial DNA, may have a key role in the energy transduction by mitochondrial H(+)-ATP synthase because an antibody against chargerin II inhibited ATP synthesis and ATP-Pi exchange, in an energy-dependent fashion....

journal_title：Biochemical and biophysical research communications

authors： Muraguchi M,Yoshihara Y,Tunemitu T,Tani I,Higuti T

更新日期：1990-04-16 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARγ) participates in the process of insulin resistance (IR), a crucial pathophysiology in non-alcoholic fatty liver disease (NAFLD). Meanwhile, suppressor of cytokine signaling3 (SOCS3) also regulates IR in NAFLD. Both PPARγ and SOCS3 play a role in NAFLD through reg...

journal_title：Biochemical and biophysical research communications

authors： Bi J,Sun K,Wu H,Chen X,Tang H,Mao J

更新日期：2018-04-15 00:00:00

abstract：:The effects of different lysophospholipids on endothelium-dependent hyperpolarization by acetylcholine were examined in rat mesenteric arteries. Lysophosphatidylcholine with 14 or longer carbon acyl chain significantly inhibited the hyperpolarization, while that with 12 or lesser carbon acyl chain was without effect. ...

journal_title：Biochemical and biophysical research communications

authors： Fukao M,Hattori Y,Kanno M,Sakuma I,Kitabatake A

更新日期：1996-10-14 00:00:00

abstract：:Cell cultures of neonatal rat cerebral cortex in a serum-free medium were used to investigate a lack of neuronotrophic factors in Alzheimer's disease (AD) brain. A few neurons survived in the absence of brain extract. The addition of normal brain extract resulted in a 2.5-fold increase in neuronal survival. AD brain e...

journal_title：Biochemical and biophysical research communications

authors： Uchida Y,Ihara Y,Tomonaga M

更新日期：1988-02-15 00:00:00

abstract：:Deficiency in the DNA mismatch repair (MMR) is frequently involved in various cancers. The hMSH3 gene is one of the human MMR genes whose role in bladder cancer is not known. We hypothesized that down-regulation of the hMSH3 gene might be involved in bladder cancer. In this study we analyzed this gene with regard to f...

journal_title：Biochemical and biophysical research communications

authors： Kawakami T,Shiina H,Igawa M,Deguchi M,Nakajima K,Ogishima T,Tokizane T,Urakami S,Enokida H,Miura K,Ishii N,Kane CJ,Carroll PR,Dahiya R

更新日期：2004-12-17 00:00:00

abstract：:The nucleotide sequence of our cloned HBV DNA (subtype adw) has been determined. When the 165-nucleotide sequence of the pre-S2 region was compared with 7 other published sequences (subtypes adw, adr, ayw, and adyw), we found 38 nucleotide substitutions among different subtypes and 4 (adr) or 6 (adw and ayw) substitut...

journal_title：Biochemical and biophysical research communications

authors： Lo SJ,Chen ML,Chien ML,Lee YH

更新日期：1986-03-13 00:00:00