Biochemical evidence for the long-tail form (A beta 1-42/43) of amyloid beta protein as a seed molecule in cerebral deposits of Alzheimer's disease.


:We measured the amounts of total A beta, A beta 1-40 and A beta 1-42/43 in brain tissues using a newly developed ELISA assay and found that the amounts of insoluble A beta 1-42/43 and insoluble A beta 1-40 were linearly related to the amount of A beta deposits or total insoluble A beta at their lower and higher concentrations, respectively. In an experiment to characterize the A beta species in brain homogenates with buffered saline, we unexpectedly detected soluble A beta which was derived from the insoluble amyloid deposits in brain tissue, indicating reversible depolymerization of A beta from insoluble amyloid deposits. To confirm this finding, we performed 5 consecutive washes of insoluble precipitates of AD brains with buffered saline. Both species of A beta were found in all 5 supernatant fractions and their amounts were gradually decreased. The ratio of A beta 1-42/43 to A beta 1-40 was increased with the numbers of washes, indicating that A beta 1-40 existed in an exposed manner as compared to A beta 1-42/43. Thus the present finding is the first biochemical evidence that A beta 1-40 was the predominant species involved in the reversible exchanging reaction on seeding A beta 1-42/43 between the soluble and the insoluble forms (amyloid fibrils).


Tamaoka A,Kondo T,Odaka A,Sahara N,Sawamura N,Ozawa K,Suzuki N,Shoji S,Mori H




Has Abstract


1994-11-30 00:00:00














  • Annexin II is a novel receptor for Pseudomonas aeruginosa.

    abstract::Infections with Pseudomonas aeruginosa (P. aeruginosa) are critical in ventilated and poly-traumatized patients. Most important, these bacteria cause frequent and chronic pulmonary infections in patients with cystic fibrosis. Therefore, identification of molecular mechanisms that mediate the infection of mammalian cel...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kirschnek S,Adams C,Gulbins E

    更新日期:2005-02-18 00:00:00

  • Phosphorylation and DNA binding of the octamer binding transcription factor Oct-3.

    abstract::Phosphorylation influences DNA binding and transactivator capabilities of multiple transcription factors. In this study, we demonstrate that the POU-domain transcription factor, Oct-3, can be phosphorylated in vivo. In addition, we show that in COS-1 cells Oct-3 is phosphorylated exclusively on serine residues. Lastly...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rosfjord E,Scholtz B,Lewis R,Rizzino A

    更新日期:1995-07-26 00:00:00

  • Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells.

    abstract::Human embryonic stem cells (hESCs) are thought to be a promising cell source for cell transplantation therapy. For such a clinical application, the hESCs should be manipulated using appropriate and qualified materials. In this study, we examined the efficacy of recombinant human laminin (rhLM) isoforms on the undiffer...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Miyazaki T,Futaki S,Hasegawa K,Kawasaki M,Sanzen N,Hayashi M,Kawase E,Sekiguchi K,Nakatsuji N,Suemori H

    更新日期:2008-10-10 00:00:00

  • TT virus is distributed in various leukocyte subpopulations at distinct levels, with the highest viral load in granulocytes.

    abstract::When TT virus (TTV) DNA was quantitated in whole blood and plasma aliquots from 27 viremic individuals by real-time detection PCR that can detect essentially all TTV genotypes, the TTV load was 6.9 +/- 3.5 (mean +/- standard deviation)-fold higher in the whole blood than in the plasma samples [P < 0.002 (paired t test...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Takahashi M,Asabe S,Gotanda Y,Kishimoto J,Tsuda F,Okamoto H

    更新日期:2002-01-11 00:00:00

  • Role of cardiolipin in the functioning of mitochondrial L-glycerol-3-phosphate dehydrogenase.

    abstract::Adriamycin was used in situ, in isolated liver mitochondria of hyperthyroid rats to study the role of cardiolipin in the functioning of FAD-linked L-glycerol-3-phosphate dehydrogenase. The apparent kinetic parameters of the reaction catalyzed by the enzyme were affected by adriamycin. The effect of adriamycin was depe...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Beleznai Z,Jancsik V

    更新日期:1989-02-28 00:00:00

  • Transcriptome analyses suggest a molecular mechanism for the SIPC response of Amphibalanus amphitrite.

    abstract::Barnacles are notorious marine fouling organisms. Their successful attachment to a substrate requires that they search for an appropriate habitat during their cyprid stage. A chemical cue called SIPC (Settlement-Inducing Protein Complex) has been shown to play a key role in the induction of cyprid gregarious settlemen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhang X,Liang C,Song J,Ye Z,Wu W,Hu B

    更新日期:2020-05-14 00:00:00

  • A novel gene encoding a smooth muscle protein is overexpressed in senescent human fibroblasts.

    abstract::In order to identify genes that may be causally involved in replicative senescence, we have isolated several gene sequences that are overexpressed in senescent human fibroblasts by differential screening of a cDNA library derived from mRNA of a subject with Werner syndrome of premature aging (Murano, S., et al., Molec...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Thweatt R,Lumpkin CK Jr,Goldstein S

    更新日期:1992-08-31 00:00:00

  • The transcription factor MEF2C mediates cardiomyocyte hypertrophy induced by IGF-1 signaling.

    abstract::Myocyte enhancer factor 2C (MEF2C) plays an important role in cardiovascular development and is a key transcription factor for cardiac hypertrophy. Here, we describe MEF2C regulation by insulin-like growth factor-1 (IGF-1) and its role in IGF-1-induced cardiac hypertrophy. We found that IGF-1 addition to cultured rat ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Muñoz JP,Collao A,Chiong M,Maldonado C,Adasme T,Carrasco L,Ocaranza P,Bravo R,Gonzalez L,Díaz-Araya G,Hidalgo C,Lavandero S

    更新日期:2009-10-09 00:00:00

  • Receptor for 1,25-dihydroxyvitamin D in a vascular smooth muscle cell line derived from rat aorta.

    abstract::The presence of a specific receptor for 1,25-dihydroxyvitamin D (1,25(OH)2D) was investigated in a cell line A7r5 derived from fetal aorta. 1,25(OH)2[3H]D3 binding to cytosol was saturated at 0.6-1 nM, and Scatchard analysis yielded dissociation constant and binding sites, (3.02 +/- 0.4) X 10(-11) M and 33.9 +/- 5.8 f...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kawashima H

    更新日期:1987-07-15 00:00:00

  • Overexpression of prothymosin alpha, concomitant with c-myc, during rat hepatic carcinogenesis.

    abstract::By using a subtraction-enhanced display technique, we identified a cDNA clone representing alpha-prothymosin, from rat hepatocellular carcinoma (HCC). alpha-Prothymosin has been reported to be involved in cell proliferation and regulated by c-myc gene in vitro. In the present study, we investigated the gene expression...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wu CG,Boers W,Reitsma PR,van Deventer SJ,Chamuleau RA

    更新日期:1997-03-27 00:00:00

  • Apoptosis induced by BIK was decreased with RNA interference of caspase-12.

    abstract::BIK, a member of the Bcl-2 family, has been reported to induce cell apoptosis but the underlying mechanisms are not well delineated. We used siRNA targeting caspase-12 to examine the effect of caspase-12 on apoptosis induced by BIK. In this study, we show that caspase-12 was activated by BIK. With caspase-12 knocked d...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhao X,Sun Y,Yu H,Ye L,Zhang L,Lu J,Yuan Y,Qian G,Ge S

    更新日期:2007-08-10 00:00:00

  • Differential expression of the transcription factor NF-kappaB during human mononuclear phagocyte differentiation to macrophages and dendritic cells.

    abstract::An important role for the Rel/NF-kappaB family of transcription factors in the differentiation process of dendritic cells (DC) and macrophages (MAC) was recently suggested by a number of mouse knockout studies but only little information is available for defined populations of human cells. To investigate the role of i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ammon C,Mondal K,Andreesen R,Krause SW

    更新日期:2000-02-05 00:00:00

  • Central effects of a novel acylated peptide, ghrelin, on growth hormone release in rats.

    abstract::Ghrelin, a novel growth-hormone-releasing acylated peptide, was recently isolated from rat stomach by the search of an endogenous ligand to an "orphan" G-protein-coupled-receptor. Ghrelin neuron is present in the arcuate nucleus of rat hypothalamus, but its central effect on growth hormone (GH) release has yet to be c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Date Y,Murakami N,Kojima M,Kuroiwa T,Matsukura S,Kangawa K,Nakazato M

    更新日期:2000-08-28 00:00:00

  • The incipient stage in thrombin-induced fibrin polymerization detected by FCS at the single molecule level.

    abstract::We used fluorescence correlation spectroscopy (FCS) to study the activation of fibrinogen by thrombin and the subsequent aggregation of fibrin monomers into fibrin polymers at a very low and at physiological fibrinogen concentrations. In the labeling procedure used the fibrinogen was randomly labeled and the label was...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Bark N,Földes-Papp Z,Rigler R

    更新日期:1999-06-24 00:00:00

  • Caspase-3 cleaves the expanded androgen receptor protein of spinal and bulbar muscular atrophy in a polyglutamine repeat length-dependent manner.

    abstract::Spinal and bulbar muscular atrophy (SBMA) is one of a group of human inherited neurodegenerative diseases caused by polyglutamine expansion. There is increasing evidence that generation of truncated proteins containing an expanded polyglutamine tract may be an important step in the pathogenesis of these disorders. We ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kobayashi Y,Miwa S,Merry DE,Kume A,Mei L,Doyu M,Sobue G

    更新日期:1998-11-09 00:00:00

  • Exposure of protein kinase motifs that trigger binding of Hsp90 and Cdc37.

    abstract::Hsp90 and its co-chaperone Cdc37 are required for the activity of numerous eukaryotic protein kinases. c-Jun N-terminal kinases (JNKs) appear to be Hsp90-independent kinases, as their activity is unaffected by Hsp90 inhibition. It is currently unknown why some protein kinases are Hsp90- and Cdc37-dependent for their f...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Prince T,Matts RL

    更新日期:2005-12-23 00:00:00

  • STAM-AMSH interaction facilitates the deubiquitination activity in the C-terminal AMSH.

    abstract::Signal transducing adaptor molecule (STAM) complexed with hepatocyte growth factor regulated tyrosine kinase substrate (Hrs) works on sorting of cargo proteins in multivesicular body (MVB) pathway. Associated molecule with SH3 domain of STAM (AMSH), a zinc-containing ubiquitin isopeptidase, is thought to play a role i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kim MS,Kim JA,Song HK,Jeon H

    更新日期:2006-12-22 00:00:00

  • Reduction in number of sarcolemmal KATP channels slows cardiac action potential duration shortening under hypoxia.

    abstract::The cardiovascular system operates under demands ranging from conditions of rest to extreme stress. One mechanism of cardiac stress tolerance is action potential duration shortening driven by ATP-sensitive potassium (K(ATP)) channels. K(ATP) channel expression has a significant physiologic impact on action potential d...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhu Z,Burnett CM,Maksymov G,Stepniak E,Sierra A,Subbotina E,Anderson ME,Coetzee WA,Hodgson-Zingman DM,Zingman LV

    更新日期:2011-12-02 00:00:00

  • Human LAT1, a subunit of system L amino acid transporter: molecular cloning and transport function.

    abstract::We report here on the cloning and functional characterization of human LAT1, a subunit of the amino acid transport system L. The hLAT1 cDNA, obtained from a human placental cDNA library, codes for a protein of 507 amino acids. When functionally expressed in mammalian cells together with the heavy chain of the rat 4F2 ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Prasad PD,Wang H,Huang W,Kekuda R,Rajan DP,Leibach FH,Ganapathy V

    更新日期:1999-02-16 00:00:00

  • Cysteine-106 of DJ-1 is the most sensitive cysteine residue to hydrogen peroxide-mediated oxidation in vivo in human umbilical vein endothelial cells.

    abstract::Mutation in DJ-1 gene is the cause of autosomal recessive Parkinson's disease, however, its physiological function remains unclear. The isoelectric point of DJ-1 shows an acidic shift after cells are treated with hydrogen peroxide. This suggests that DJ-1 is modified in response to oxidative stress. Here we report the...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kinumi T,Kimata J,Taira T,Ariga H,Niki E

    更新日期:2004-05-07 00:00:00

  • Human neutrophils store type II 14-kDa phospholipase A2 in granules and secrete active enzyme in response to soluble stimuli.

    abstract::Although "secretory" type II 14-kDa phospholipase A2 (sPLA2) activity has been described in neutrophils, direct evidence of enzyme secretion has been elusive. We have used immunogold electron microscopy with polyclonal and monoclonal antibodies to sPLA2 to demonstrate localization of the enzyme to granules of resting ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rosenthal MD,Gordon MN,Buescher ES,Slusser JH,Harris LK,Franson RC

    更新日期:1995-03-17 00:00:00

  • Occurrence of 4-hydroxyalkenals in rat tissues determined as pentafluorobenzyl oxime derivatives by gas chromatography-mass spectrometry.

    abstract::Malondialdehyde measurements have been the major tool for studying relationships between lipid peroxidation and tissue pathology. Recently, we presented a novel gas chromatography-mass spectrometry method for direct detection of phospholipid peroxides with picogram sensitivity based on transesterification of phospholi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: van Kuijk FJ,Thomas DW,Stephens RJ,Dratz EA

    更新日期:1986-08-29 00:00:00

  • Human cellular src gene product: identification of the myristoylated pp60c-src and blockage of its myristoyl acylation with N-fatty acyl compounds resulted in the suppression of colony formation.

    abstract::A p60K protein in human colon adenocarcinoma tumor cell lines was identified as a myristoylated pp60c-src by fluorography and radioimmunoprecipitation analysis. Prevention of the myristoylation of pp60c-src was determined with N-fatty acyl glycinal compounds. Of the compounds tested, N-myristoyl glycinal diethylacetal...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Shoji S,Kurosawa T,Inoue H,Funakoshi T,Kubota Y

    更新日期:1990-12-31 00:00:00

  • Toll-like receptor 4 protects against irradiation-induced hematopoietic injury by promoting granulopoiesis and alleviating marrow adipogenesis.

    abstract::Irradiation induces severe damage in the hematopoietic system, which leads to bone marrow hyperplasia, pancytopenia, and aggravated tissue formation in bone marrow. Studies have shown that Toll-like receptor 4 (TLR4) has a protective effect against irradiation, but the underlying mechanism remains unclear. In this stu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sun Z,Wu K,Lin Q,Fei H,Jiang H,Chen T,Yuan Y

    更新日期:2019-12-03 00:00:00

  • Mitochondria and microsomal membranes have a free radical reductase activity that prevents chromanoxyl radical accumulation.

    abstract::Enzyme-dependent mechanisms which prevent accumulation of chromanoxyl radicals derived from the vitamin E analogue, 2,2,5,7,8-pentamethyl-6-hydroxycromane (PMC), were characterized in rat liver microsomal and mitochondrial membranes. The free radical oxidation product of PMC (chromanoxyl radical) was generated in memb...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Packer L,Maguire JJ,Mehlhorn RJ,Serbinova E,Kagan VE

    更新日期:1989-02-28 00:00:00

  • Pak regulates calpain-dependent degradation of E3b1.

    abstract::E3b1, a binding partner of Eps8, plays a critical role in receptor tyrosine kinase (RTK)-mediated Rac activation by facilitating the interaction of Eps8 with Sos-1 and the consequent activation of the Rac-specific guanine nucleotide exchange factor activity of Sos-1. Here we present evidence that E3b1 levels are regul...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chi S,Chang S,Park D

    更新日期:2004-06-25 00:00:00

  • Iron at the cell surface controls DNA synthesis in CCl 39 cells.

    abstract::Treatment of CCl 39 cells with the impermeable iron II chelator bathophenanthroline disulfonate (BPS) inhibits both DNA synthesis and transplasma membrane electron transport. The inhibition persists when the BPS is removed, and the extract from 10(6) cells contains up to 1.28 nmoles iron II chelated to BPS. The BPS ir...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Alcain FJ,Löw H,Crane FL

    更新日期:1994-08-30 00:00:00

  • Crystal structure and biochemical characterization of malate dehydrogenase from Metallosphaera sedula.

    abstract::Metallosphaera sedula is a thermoacidophilic autotrophic archaeon and known to utilize the 3-hydroxypropionate/4-hydroxybutyrate cycle (3-HP/4-HB cycle) as a carbon fixation pathway. The 3-HP/4-HB cycle in M. sedula is associated with central metabolism, and malate dehydrogenase (MDH) is an enzyme involved in the cent...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lee D,Hong J,Kim KJ

    更新日期:2019-02-12 00:00:00

  • Mechanism of cytotoxicity of aflatoxin B1: role of cytochrome P1-450.

    abstract::A mouse hepatoma cell line, Hepa-1, is highly sensitive to the toxic effects of Aflatoxin B1 (AFB1). Half maximal survival (LD50) of cells occurs at 0.068 ug AFB1/ml. Benzo(a)anthracene, which induces aryl hydrocarbon hydroxylase and cytochrome P1-450 in Hepa-1, causes a slight increase in the toxicity of AFB1 (LD50 =...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kärenlampi SO

    更新日期:1987-06-15 00:00:00

  • Oxidative stress involved in the antibacterial action of different antibiotics.

    abstract::Staphylococcus aureus and Escherichia coli sensitive to chloramphenicol incubated with this antibiotic suffered oxidative stress with increase of anion superoxide (O2-). This reactive species of oxygen was detected by chemiluminescence with lucigenin. S. aureus, E. coli, and Enterococcus faecalis sensitive to ciproflo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Albesa I,Becerra MC,Battán PC,Páez PL

    更新日期:2004-04-30 00:00:00