Effect of thiomersal on dissociation of intact (146S) foot-and-mouth disease virions into 12S particles as assessed by novel ELISAs specific for either 146S or 12S particles.


:Intact (146S) foot-and-mouth disease virions (FMDVs) can dissociate into specific (12S) viral capsid degradation products. Using two single-domain antibody fragments that bind specifically to either 146S or 12S particles we developed two ELISAs for the quantification of these particles in FMDV antigen preparations used for vaccine manufacturing. Only O serotype strains are detected in the 146S specific ELISA whereas strains of most serotypes are detected in the 12S specific ELISA. However, the 146S concentration of A and Asia 1 serotype strains could be measured indirectly using the 12S specific ELISA by prior conversion of 146S into 12S particles by heat treatment. This allowed us to demonstrate that addition of the preservative thiomersal to FMDV antigens stimulates the dissociation into 12S particles of O, A and Asia 1 serotype strains upon prolonged storage at 4°C. FMDV dissociation is known to result in a strongly reduced immunogenicity, which was experimentally confirmed here. Therefore, we recommend to omit thiomersal from FMD vaccines to increase its shelf life.






Harmsen MM,Fijten HP,Westra DF,Coco-Martin JM




Has Abstract


2011-03-24 00:00:00
















  • An improved Pythium insidiosum-vaccine formulation with enhanced immunotherapeutic properties in horses and dogs with pythiosis.

    abstract::The immunotherapeutic properties of a new Pythium insidiosum-vaccine formulation (PIV), was evaluated in 18 horses and 6 dogs with proven pythiosis from different enzootic areas in the United States. All injected horses but one responded with a weak (=29 mm, n=3), a mild (30-90 mm, n=7) or a strong (=100 mm, n=7) infl...


    pub_type: 杂志文章


    authors: Mendoza L,Mandy W,Glass R

    更新日期:2003-06-20 00:00:00

  • Protection of pigs against Taenia solium cysticercosis using recombinant antigen or in combination with DNA vaccine.

    abstract::In the present study, we investigated the duration of protection afforded to pigs immunized in two different prime-boost regimens: one is homologus priming and boosting with a protein vaccine, and the other is priming with a DNA vaccine and boosting with the protein vaccine. Groups of pigs that received the same vacci...


    pub_type: 杂志文章


    authors: Guo YJ,Sun SH,Zhang Y,Chen ZH,Wang KY,Huang L,Zhang S,Zhang HY,Wang QM,Wu D,Zhu WJ

    更新日期:2004-09-28 00:00:00

  • Influence of live respiratory syncytial virus priming on the immune response generated by a recombinant vaccine candidate, BBG2Na.

    abstract::Respiratory syncytial virus is one of the major respiratory pathogens for infants and immunocompromized children. With the exception of young children, all the population has encountered RSV and is seropositive. Recent reports have demonstrated however that the virus also affects the elderly and represents a major cau...


    pub_type: 杂志文章


    authors: Goestch L,Plotnicky-Gilquin H,Champion T,Beck A,Haeuw JF,Nguyen T,Bonnefoy JY,Corvaïa N

    更新日期:2000-06-01 00:00:00

  • Critical issues in cancer vaccine trial design.

    abstract::As the clinical experience with cancer vaccines and cancer immunotherapy increases, there are important lessons that can be learned from the successes and failures of past trials. Many lessons affect the design and conduct of clinical trials themselves. Appropriate patient selection, clinical trial design, immunologic...


    pub_type: 杂志文章,评审


    authors: Clifton GT,Kohrt HE,Peoples GE

    更新日期:2015-12-16 00:00:00

  • Tracking parental attitudes on vaccination across European countries: The Vaccine Safety, Attitudes, Training and Communication Project (VACSATC).

    abstract::The paper presents the first results from the European project VACSATC which aimed to track parental attitudes on vaccinations across several European countries. We compared five cross-sectional surveys of parents with children less than 3 years of age in England, Norway, Poland, Spain and Sweden carried out during 20...


    pub_type: 杂志文章


    authors: Stefanoff P,Mamelund SE,Robinson M,Netterlid E,Tuells J,Bergsaker MA,Heijbel H,Yarwood J,VACSATC working group on standardization of attitudinal studies in Europe.

    更新日期:2010-08-09 00:00:00

  • No effect of an additional early dose of measles vaccine on hospitalization or mortality in children: A randomized controlled trial.

    abstract:BACKGROUND:Non-specific effects (NSEs) of vaccines have increasingly gained attention in recent years. Recent studies suggest that live vaccines, such as measles vaccine (MV), have beneficial effects on health, while inactivated vaccines, such as the diphtheria-tetanus-pertussis (DTP) vaccine, may have harmful effects....


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Schoeps A,Nebié E,Fisker AB,Sié A,Zakane A,Müller O,Aaby P,Becher H

    更新日期:2018-04-05 00:00:00

  • Placebo use in vaccine trials: recommendations of a WHO expert panel.

    abstract::Vaccines are among the most cost-effective interventions against infectious diseases. Many candidate vaccines targeting neglected diseases in low- and middle-income countries are now progressing to large-scale clinical testing. However, controversy surrounds the appropriate design of vaccine trials and, in particular,...


    pub_type: 杂志文章


    authors: Rid A,Saxena A,Baqui AH,Bhan A,Bines J,Bouesseau MC,Caplan A,Colgrove J,Dhai A,Gomez-Diaz R,Green SK,Kang G,Lagos R,Loh P,London AJ,Mulholland K,Neels P,Pitisuttithum P,Sarr SC,Selgelid M,Sheehan M,Smith PG

    更新日期:2014-08-20 00:00:00

  • Changes in the seroepidemiology of hepatitis B infection in Catalonia 1989-1996.

    abstract::The objective of this study was to investigate the prevalence of hepatitis B markers in a representative sample of 2142 subjects in Catalonia, Spain, and to compare it with previous studies. Multiple logistical regression analysis was carried out to determine variables associated with the markers studied. The prevalen...


    pub_type: 杂志文章


    authors: Domínguez A,Bruguera M,Vidal J,Plans P,Salleras L

    更新日期:2000-05-08 00:00:00

  • Recombinant protein vaccines produced in insect cells.

    abstract::The baculovirus-insect cell expression system is a well known tool for the production of complex proteins. The technology is also used for commercial manufacture of various veterinary and human vaccines. This review paper provides an overview of how this technology can be applied to produce a multitude of vaccine cand...


    pub_type: 杂志文章,评审


    authors: Cox MM

    更新日期:2012-02-27 00:00:00

  • National surveillance of invasive pneumococcal diseases in Taiwan, 2008-2012: differential temporal emergence of serotype 19A.

    abstract:INTRODUCTION:Serotype replacement after the introduction of seven-valent pneumococcal conjugate vaccine (PCV7) and the future availability of multivalent PCVs prompted the listing of invasive pneumococcal disease (IPD) as a notifiable disease in Taiwan in October 2007. Here, we report the national surveillance results....


    pub_type: 杂志文章


    authors: Chiang CS,Chen YY,Jiang SF,Liu DP,Kao PH,Teng HJ,Kuo TL,Yao SM,Tseng LR,Wang YL,Wu HS,Chang FY,Lin TY

    更新日期:2014-06-05 00:00:00

  • TLR-based immune adjuvants.

    abstract::This work describes the nature and strength of the immune response induced by various Toll-like receptor ligands and their ability to act as vaccine adjuvants. It reviews the various ligands capable of triggering individual TLRs, and then focuses on the efficacy and safety of those agents for which clinical results ar...


    pub_type: 杂志文章,评审


    authors: Steinhagen F,Kinjo T,Bode C,Klinman DM

    更新日期:2011-04-12 00:00:00

  • Preclinical refinements of a broadly protective VLP-based HPV vaccine targeting the minor capsid protein, L2.

    abstract::An ideal prophylactic human papillomavirus (HPV) vaccine would provide broadly protective and long-lasting immune responses against all high-risk HPV types, would be effective after a single dose, and would be formulated in such a manner to allow for long-term storage without the necessity for refrigeration. We have d...


    pub_type: 杂志文章


    authors: Tumban E,Muttil P,Escobar CA,Peabody J,Wafula D,Peabody DS,Chackerian B

    更新日期:2015-06-26 00:00:00

  • Economic and clinical evaluation of a catch-up dose of 13-valent pneumococcal conjugate vaccine in children already immunized with three doses of the 7-valent vaccine in Italy.

    abstract::A new 13-valent conjugated polysaccharide vaccine (PCV13) against Streptococcus pneumoniae infections, which replaced the 7-valent vaccine (PCV7) in the regional immunization programmes for newborns and children who started but not completed the 3 doses schedule of PCV7, is available in Italy since 2010. The opportuni...


    pub_type: 杂志文章


    authors: Boccalini S,Azzari C,Resti M,Valleriani C,Cortimiglia M,Tiscione E,Bechini A,Bonanni P

    更新日期:2011-11-28 00:00:00

  • Recombinant CP40 from Corynebacterium pseudotuberculosis confers protection in mice after challenge with a virulent strain.

    abstract:BACKGROUND:Caseous Lymphadenitis (CLA) is a contagious, infectious, chronic disease caused by Corynebacterium pseudotuberculosis, which affects mainly sheep and goats. The clinical prevalence of CLA in Brazil is 30%, resulting in decreased milk production, weight loss, and unusable meat and leather. Prophylaxis is base...


    pub_type: 杂志文章


    authors: Droppa-Almeida D,Vivas WL,Silva KK,Rezende AF,Simionatto S,Meyer R,Lima-Verde IB,Delagostin O,Borsuk S,Padilha FF

    更新日期:2016-02-17 00:00:00

  • Gene gun DNA immunization of cattle induces humoral and CD4 T-cell-mediated immune responses against the Theileria parva polymorphic immunodominant molecule.

    abstract::Theileria parva kills over one million cattle annually in sub-Saharan Africa. Parasite genetic complexity, cellular response immunodominance, and bovine MHC diversity have precluded traditional vaccine development. One potential solution is gene gun (GG) immunization, which enables simultaneous administration of one o...


    pub_type: 杂志文章


    authors: Fry LM,Bastos RG,Stone BC,Williams LB,Knowles DP,Murphy SC

    更新日期:2019-03-14 00:00:00

  • Response, tolerance and ignorance following oral exposure to a single dietary protein antigen in Gallus domesticus.

    abstract::The objective of this investigation was to analyze conditions leading to antibody responses against innocuous dietary protein antigens in the chick. The physical form of antigen was found to be important for immunization of mature chicks: bovine serum albumin (BSA) in solution was a powerful immunogen, while BSA powde...


    pub_type: 杂志文章


    authors: Klipper E,Sklan D,Friedman A

    更新日期:2001-04-06 00:00:00

  • A randomized controlled trial of an online immunization curriculum.

    abstract:INTRODUCTION:Immunization education for physicians-in-training is crucial to address vaccine concerns in clinical practice. Vaccine education is not standardized across residency programs. The Collaboration for Vaccination Education and Research (CoVER) team developed an online curriculum for pediatric (Peds) and famil...


    pub_type: 杂志文章


    authors: Pahud B,Elizabeth Williams S,Lee BR,Lewis KO,Middleton DB,Clark S,Humiston SG

    更新日期:2020-10-27 00:00:00

  • Immunopotentiating heat shock proteins: negotiators between innate danger and control of autoimmunity.

    abstract::Heat shock proteins (hsps) are known to be immunodominant antigens of bacteria. Hsps are evolutionarily strongly conserved proteins present in all eukaryotic and prokaryotic cellular organisms and upregulated by several forms of stress. Despite (the paradigm of) self-tolerance, hsp-epitopes homologous to endogenous ho...


    pub_type: 杂志文章,评审


    authors: van Eden W,Koets A,van Kooten P,Prakken B,van der Zee R

    更新日期:2003-02-14 00:00:00

  • Smallpox and its eradication in the Democratic Republic of Congo: lessons learned.

    abstract::Smallpox eradication is considered to be one of the most remarkable accomplishments of the 20th century. Lessons learned from the campaign during the 1960s and 1970s in the Democratic Republic of Congo (DRC) can provide important information for the development of other eradication programs including polio. The DRC is...


    pub_type: 历史文章,杂志文章,评审


    authors: Muyembe-Tamfum JJ,Mulembakani P,Lekie RB,Szczeniowski M,Ježek Z,Doshi R,Hoff N,Rimoin AW

    更新日期:2011-12-30 00:00:00

  • Immunogenicity and protective efficacy of a novel foot-and-mouth disease virus empty-capsid-like particle with improved acid stability.

    abstract::Foot-and-mouth disease virus (FMDV) is the etiological agent of a highly contagious disease that affects cloven-hoofed animal species. The FMDV capsid is highly acid labile and viral particles lose their immunogenicity when they disassemble at mildly acidic pHs. The viral capsid of FMDV serotype O is more sensitive th...


    pub_type: 杂志文章


    authors: Xie Y,Li H,Qi X,Ma Y,Yang B,Zhang S,Chang H,Yin X,Li Z

    更新日期:2019-03-28 00:00:00

  • Safety, immunogenicity and shedding of LAIV4 in HIV-infected and uninfected children.

    abstract:OBJECTIVES:HIV-infected children have poor responses to inactivated influenza vaccines. Live vaccines (LAIVs) are highly efficacious in children, but they are not used in HIV-infected children du e to limited information. We investigated the safety, immunogenicity and viral shedding of LAIV4 in HIV-infected compared wi...


    pub_type: 杂志文章


    authors: Curtis D,Ning MF,Armon C,Li S,Weinberg A

    更新日期:2015-09-11 00:00:00

  • Vaccination coverage against hepatitis A and B viruses, Streptococcus pneumoniae, seasonal flu, and A(H1N1)2009 pandemic influenza in HIV-infected patients.

    abstract:BACKGROUND:Several vaccines are recommended in HIV-infected patients due to an increased risk of vaccine-preventable infections, severe forms of the disease, or shared transmission routes. Few data are available regarding vaccination coverage and its determinants in this population. METHODS:A cross-sectional study was...


    pub_type: 杂志文章


    authors: Valour F,Cotte L,Voirin N,Godinot M,Ader F,Ferry T,Vanhems P,Chidiac C

    更新日期:2014-07-31 00:00:00

  • Phylogenetic and antigenic analyses of coxsackievirus A6 isolates in Yamagata, Japan between 2001 and 2017.

    abstract::Although coxsackievirus A6 (CV-A6) is generally recognized as a causative agent of herpangina in children, CV-A6 infections globally emerged as a new and major cause of epidemic hand-foot-and-mouth-diseases (HFMDs) around 2008. To clarify the longitudinal epidemiology of CV-A6, we carried out sequence and phylogenetic...


    pub_type: 杂志文章


    authors: Mizuta K,Tanaka S,Komabayashi K,Aoki Y,Itagaki T,Katsushima F,Katsushima Y,Yoshida H,Ito S,Matsuzaki Y,Ikeda T

    更新日期:2019-02-14 00:00:00

  • Safety of 9-valent human papillomavirus vaccine administration among pregnant women: Adverse event reports in the Vaccine Adverse Event Reporting System (VAERS), 2014-2017.

    abstract:INTRODUCTION:9-valent human papillomavirus vaccine (9vHPV) was approved by the Food and Drug Administration (FDA) in December 2014. 9vHPV is not recommended during pregnancy, but some women of childbearing age may be inadvertently exposed. This study aims to evaluate reports submitted to the Vaccine Adverse Event Repor...


    pub_type: 杂志文章


    authors: Landazabal CS,Moro PL,Lewis P,Omer SB

    更新日期:2019-02-21 00:00:00

  • Dengue tetravalent DNA vaccine increases its immunogenicity in mice when mixed with a dengue type 2 subunit vaccine or an inactivated Japanese encephalitis vaccine.

    abstract::We previously developed a dengue tetravalent DNA vaccine that can induce neutralizing antibodies against four dengue viruses in mice. Here, we demonstrated that immunogenicity of our tetravalent vaccine is synergistically increased in mice by co-immunization with dengue type 2 virus (DENV2) subviral extracellular part...


    pub_type: 杂志文章


    authors: Imoto J,Konishi E

    更新日期:2007-01-22 00:00:00

  • Ag85B-ESAT-6 adjuvanted with IC31 promotes strong and long-lived Mycobacterium tuberculosis specific T cell responses in naïve human volunteers.

    abstract::Though widely used, the BCG vaccine has had little apparent effect on rates of adult pulmonary tuberculosis. Moreover, the risk of disseminated BCG disease in immunocompromised individuals means that improved TB vaccines ideally need to be able to efficiently prime mycobacterially-naïve individuals as well as boost in...


    pub_type: 杂志文章,随机对照试验


    authors: van Dissel JT,Arend SM,Prins C,Bang P,Tingskov PN,Lingnau K,Nouta J,Klein MR,Rosenkrands I,Ottenhoff TH,Kromann I,Doherty TM,Andersen P

    更新日期:2010-04-30 00:00:00

  • Non PC liposome entrapped promastigote antigens elicit parasite specific CD8+ and CD4+ T-cell immune response and protect hamsters against visceral leishmaniasis.

    abstract::Leishmania donovani promastigote soluble antigens (sLAg) were encapsulated in non-phosphatidylcholine (non-PC) liposomes (escheriosomes) prepared from E. coli lipids. The escheriosome-based vaccine was investigated for its potential to elicit a protective immune response against experimental visceral leishmaniasis. Th...


    pub_type: 杂志文章


    authors: Sharma SK,Dube A,Nadeem A,Khan S,Saleem I,Garg R,Mohammad O

    更新日期:2006-03-10 00:00:00

  • Effectiveness of Lanzhou lamb rotavirus vaccine against rotavirus gastroenteritis requiring hospitalization: a matched case-control study.

    abstract::We sought to determine the vaccine effectiveness (VE) of Lanzhou lamb rotavirus (LLR) vaccine against rotavirus gastroenteritis in children <5 requiring hospitalization. Children hospitalized 2002-2004 with rotavirus gastroenteritis were matched by gender, age and community to 838 controls. VE was calculated for one o...


    pub_type: 杂志文章


    authors: Fu C,Wang M,Liang J,He T,Wang D,Xu J

    更新日期:2007-12-17 00:00:00

  • Meningitis epidemics in Africa: a brief overview.

    abstract::Every year, meningococcal meningitis causes thousands of deaths within the meningitis belt in sub-Saharan African countries. Large epidemic waves occur with a periodicity of 5-12 years. The waves do correspond to molecular changes in the expression of capsular or subcapsular antigens, which allow the bug to spread in ...


    pub_type: 杂志文章,评审


    authors: Teyssou R,Muros-Le Rouzic E

    更新日期:2007-09-03 00:00:00

  • Systemic immunization with CCL27/CTACK modulates immune responses at mucosal sites in mice and macaques.

    abstract::Plasmid DNA is a promising vaccine platform that has been shown to be safe and able to be administered repeatedly without vector interference. Enhancing the potency of DNA vaccination through co-delivery of molecular adjuvants is one strategy currently under investigation. Here we describe the use of the novel chemoki...


    pub_type: 杂志文章


    authors: Kraynyak KA,Kutzler MA,Cisper NJ,Khan AS,Draghia-Akli R,Sardesal NY,Lewis MG,Yan J,Weiner DB

    更新日期:2010-02-23 00:00:00