Effect of thiomersal on dissociation of intact (146S) foot-and-mouth disease virions into 12S particles as assessed by novel ELISAs specific for either 146S or 12S particles.


:Intact (146S) foot-and-mouth disease virions (FMDVs) can dissociate into specific (12S) viral capsid degradation products. Using two single-domain antibody fragments that bind specifically to either 146S or 12S particles we developed two ELISAs for the quantification of these particles in FMDV antigen preparations used for vaccine manufacturing. Only O serotype strains are detected in the 146S specific ELISA whereas strains of most serotypes are detected in the 12S specific ELISA. However, the 146S concentration of A and Asia 1 serotype strains could be measured indirectly using the 12S specific ELISA by prior conversion of 146S into 12S particles by heat treatment. This allowed us to demonstrate that addition of the preservative thiomersal to FMDV antigens stimulates the dissociation into 12S particles of O, A and Asia 1 serotype strains upon prolonged storage at 4°C. FMDV dissociation is known to result in a strongly reduced immunogenicity, which was experimentally confirmed here. Therefore, we recommend to omit thiomersal from FMD vaccines to increase its shelf life.






Harmsen MM,Fijten HP,Westra DF,Coco-Martin JM




Has Abstract


2011-03-24 00:00:00
















  • More vaccines for children? Parents' views.

    abstract:AIM:To investigate parents' views regarding potential new vaccines. We examined attitudes towards severity of specific infections, acceptability of potential vaccines and preferences for the number of injections they would want their child to receive on any one occasion. DESIGN:Cross sectional survey. METHODS:Parents...


    pub_type: 杂志文章


    authors: Bedford H,Lansley M

    更新日期:2007-11-07 00:00:00

  • Efficacy of a human anthrax vaccine in guinea pigs, rabbits, and rhesus macaques against challenge by Bacillus anthracis isolates of diverse geographical origin.

    abstract::The efficacy of a licensed human anthrax vaccine (Anthrax Vaccine Adsorbed (AVA)) was tested in guinea pigs, rabbits, and rhesus macaques against spore challenge by Bacillus anthracis isolates of diverse geographical origin. Initially, groups of Hartley guinea pigs were vaccinated at 0 and 4 weeks with AVA, then chall...


    pub_type: 杂志文章


    authors: Fellows PF,Linscott MK,Ivins BE,Pitt ML,Rossi CA,Gibbs PH,Friedlander AM

    更新日期:2001-04-30 00:00:00

  • Protection of swine from foot-and-mouth disease with one dose of an all-D retro peptide.

    abstract::Nine pigs were given a single inoculum of 100 microg of the all-D retro peptide corresponding to the immunodominant GH loop encompassing residues 141-159 of capsid protein VP1 of foot-and-mouth disease virus serotype A, sub-type 12. The peptide was conjugated to activated keyhole limpet haemocyanin and oil-adjuvanted ...


    pub_type: 杂志文章


    authors: Nargi F,Kramer E,Mezencio J,Zamparo J,Whetstone C,Van Regenmortel MH,Briand JP,Muller S,Brown F

    更新日期:1999-07-16 00:00:00

  • No major outbreaks of pseudorabies virus in well-immunized sow herds.

    abstract::In this study we quantified the transmission of pseudorabies virus (PRV) in well-immunized sow herds in The Netherlands. In three herds, sows were tested for antibodies to gE of PRV every time after they had been transported to another barn (survey A). In 99 other herds, sows were tested simultaneously once or twice y...


    pub_type: 杂志文章


    authors: Van Nes A,Stegeman JA,De Jong MC,Loeffen WL,Kimman TG,Verheijden JH

    更新日期:1996-08-01 00:00:00

  • Monitoring of rotavirus vaccination in Morocco: establishing the baseline burden of rotavirus disease.

    abstract:BACKGROUND:Rotavirus is a leading cause of childhood morbidity and mortality worldwide. Clinical trials for two rotavirus vaccines recommended by the WHO for global use since 2009 have successfully demonstrated the safety and efficacy of these vaccines in a wide range of countries. To control the burden of severe and f...


    pub_type: 杂志文章


    authors: Benhafid M,Rguig A,Trivedi T,Elqazoui M,Teleb N,Mouane N,Maltouf AF,Parashar U,Patel M,Aouad RE

    更新日期:2012-10-12 00:00:00

  • Selection bias in evaluating of influenza vaccine effectiveness: a lesson from an observational study of elderly nursing home residents.

    abstract::Selection bias is of critical concern in the study of influenza vaccine effectiveness when using an observational study design. This bias is attributable to the inherently different characteristics between vaccinees and non-vaccinees. The differences, which are related both to vaccination and signs of clinical disease...


    pub_type: 杂志文章


    authors: Fukushima W,Hayashi Y,Mizuno Y,Suzuki K,Kase T,Ohfuji S,Fujieda M,Maeda A,Hirota Y

    更新日期:2008-11-25 00:00:00

  • Simple differentiation method of mumps Hoshino vaccine strain from wild strains by reverse transcription loop-mediated isothermal amplification (RT-LAMP).

    abstract::Mumps virus is still circulating and annual mumps outbreaks occur with fluctuating magnitudes in Japan. Aseptic meningitis has been reported after vaccination and it would be of importance to determine whether this was related to the vaccination. The objective of this study was to develop a sensitive, specific and rap...


    pub_type: 杂志文章


    authors: Yoshida N,Fujino M,Ota Y,Notomi T,Nakayama T

    更新日期:2007-01-26 00:00:00

  • Recombinant Ag85B vaccine by taking advantage of characteristics of human parainfluenza type 2 virus vector showed Mycobacteria-specific immune responses by intranasal immunization.

    abstract::Viral vectors are promising vaccine candidates for eliciting suitable Ag-specific immune response. Since Mycobacterium tuberculosis (Mtb) normally enters hosts via the mucosal surface of the lung, the best defense against Mtb is mucosal vaccines that are capable of inducing both systemic and mucosal immunity. Although...


    pub_type: 杂志文章


    authors: Watanabe K,Matsubara A,Kawano M,Mizuno S,Okamura T,Tsujimura Y,Inada H,Nosaka T,Matsuo K,Yasutomi Y

    更新日期:2014-03-26 00:00:00

  • The recombinant Lactococcus lactis oral vaccine induces protection against C. difficile spore challenge in a mouse model.

    abstract::Clostridium difficile infection (CDI) causes nosocomial antibiotic-associated diarrhea and colitis in the developed world. Two potent cytotoxins, toxin A (TcdA) and toxin B (TcdB) are the virulence factors of this disease and can be a good vaccine candidate against CDI. In the present study, we genetically engineered ...


    pub_type: 杂志文章


    authors: Guo S,Yan W,McDonough SP,Lin N,Wu KJ,He H,Xiang H,Yang M,Moreira MA,Chang YF

    更新日期:2015-03-24 00:00:00

  • HBV-vaccination in recipients of kidney allografts.

    abstract::Recipients of renal transplants were vaccinated with 20 ng hepatitis B surface antigen aluminium-adsorbed vaccine in order to gauge their resistance to hepatitis B infection, to which these patients are at high risk. The patients were given three 40 micrograms doses of the vaccine over a period of six months and their...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Feuerhake A,Muller R,Lauchart W,Pichlmayr R,Schmidt FW

    更新日期:1984-12-01 00:00:00

  • Classification of measles cases from 2014 to 2018: Implications for progress towards measles elimination in China.

    abstract::Measles incidence has decreased dramatically in China due to the implement of measles-containing vaccine (MCV). However, a measles epidemic caused resurgence recently, even among vaccinated individuals. To evaluate the effectiveness of current immunization programs and discuss initiatives for the next step in measles ...


    pub_type: 杂志文章


    authors: Cui A,Zhang Y,Zhu Z,Wang H,Mao N,Song J,Xu W

    更新日期:2020-05-08 00:00:00

  • Impact of the 10-valent pneumococcal non-typeable Haemophilus influenzae Protein D conjugate vaccine (PHiD-CV) on bacterial nasopharyngeal carriage.

    abstract::Pneumococcal conjugate vaccines (PCV) may reduce nasopharyngeal carriage (NPC) of Streptococcus pneumoniae vaccine strains (VT), but serotype replacement with non-vaccine strains (NVT) has been reported. Bacterial NPC after PHiD-CV vaccination was assessed in the second year of life. Open descriptive study of NPC repo...


    pub_type: 杂志文章


    authors: Prymula R,Hanovcova I,Splino M,Kriz P,Motlova J,Lebedova V,Lommel P,Kaliskova E,Pascal T,Borys D,Schuerman L

    更新日期:2011-02-24 00:00:00

  • Purification and characterization of Epstein-Barr virus gp340/220 produced by a bovine papillomavirus virus expression vector system.

    abstract::Our initial results with a bovine papilloma virus (BPV) vector expression system indicated that we could produce significant amounts of Epstein-Barr virus (EBV) gp340/220 in the supernatant of a mouse fibroblast cell line. We have now extended these findings to show that the truncated version of gp340/220, where the m...


    pub_type: 杂志文章


    authors: Madej M,Conway MJ,Morgan AJ,Sweet J,Wallace L,Qualtiere LF,Arrand JR,Mackett M

    更新日期:1992-01-01 00:00:00

  • Studies on recombinant glucokinase (r-glk) protein of Brucella abortus as a candidate vaccine molecule for brucellosis.

    abstract::Brucellosis is one of the most prevalent zoonotic diseases of worldwide distribution caused by the infection of genus Brucella. Live attenuated vaccines such as B. abortus S19, B. abortus RB51 and B. melitensis Rev1 are found most effective against brucellosis infection in animals, contriving a number of serious side ...


    pub_type: 杂志文章


    authors: Vrushabhendrappa,Singh AK,Balakrishna K,Sripathy MH,Batra HV

    更新日期:2014-09-29 00:00:00

  • Oral tolerance elicited in mice by beta-lactoglobulin entrapped in biodegradable microspheres.

    abstract::Oral administration of antigen is known to be appropriate for some vaccine purposes as well as oral tolerance induction. In the present study, oral administration of beta-lactoglobulin (BLG) loaded poly(D,L-lactide-co-glycolide) (D,L-PLG) microspheres induced tolerance was evaluated. A single feeding of 5 micrograms o...


    pub_type: 杂志文章


    authors: Pecquet S,Leo E,Fritsché R,Pfeifer A,Couvreur P,Fattal E

    更新日期:2000-01-18 00:00:00

  • Cost-effectiveness analysis of pneumococcal conjugate vaccination in England and Wales.

    abstract:AIM:To establish whether universal vaccination of infants with the pneumococcal conjugate vaccine is likely to be cost-effective from the perspective of the health care provider (NHS). METHOD:Two hypothetical cohorts--one vaccinated and one unvaccinated--were followed over their lifetime, and the expected net costs an...


    pub_type: 杂志文章


    authors: Melegaro A,Edmunds WJ

    更新日期:2004-10-22 00:00:00

  • Nef modulates the immunogenicity of Gag encoded in a non-infectious HIV DNA vaccine.

    abstract::Gag-CD8+ T cell responses are associated with immune control of HIV infection. Since during HIV infection Nef impairs T cell responses, we evaluated whether deletion of nef from a non-infectious HIV DNA vaccine (Delta4 Nef+), creating Delta5 Nef(-), would affect its immunogenicity. When compared with Delta4, mice inje...


    pub_type: 杂志文章


    authors: Arrode G,Hegde R,Jin Y,Singh DK,Narayan O,Chebloune Y

    更新日期:2008-07-23 00:00:00

  • Parents' preferences for seasonal influenza vaccine for their children in Japan.

    abstract::In Japan, trivalent inactivated influenza vaccine is the only approved influenza vaccine. It is typically administrated by hypodermic injection, and children under 13 years of age are recommended to be vaccinated two times during each winter season. Live-attenuated influenza vaccine (LAIV) is administered by a thimero...


    pub_type: 杂志文章


    authors: Shono A,Kondo M

    更新日期:2014-09-03 00:00:00

  • Safety and immunogenicity in volunteers of a recombinant Plasmodium falciparum circumsporozoite protein malaria vaccine produced in Lepidopteran cells.

    abstract::A recombinant Plasmodium falciparum circumsporozoite (CS) antigen (rPfCSA) was produced in insect cells using a baculovirus expression vector containing the entire CS gene. This near full-length CS antigen was adsorbed onto aluminium phosphate for use as a malaria vaccine. In a study of safety and immunogenicity, 20 v...


    pub_type: 临床试验,杂志文章


    authors: Herrington DA,Losonsky GA,Smith G,Volvovitz F,Cochran M,Jackson K,Hoffman SL,Gordon DM,Levine MM,Edelman R

    更新日期:1992-01-01 00:00:00

  • Vaccination of sheep against M. paratuberculosis: immune parameters and protective efficacy.

    abstract::Johne's disease in ruminants is caused by the pathogenic bacterium Mycobacterium avium subspecies paratuberculosis (Map). Currently available Map commercial vaccines protect against clinical disease but not infection. In this study, the proprietary Johne's vaccine Neoparasec and an aqueous formulation of Map 316F (Aqu...


    pub_type: 临床试验,杂志文章


    authors: Begg DJ,Griffin JF

    更新日期:2005-10-10 00:00:00

  • Primary in vitro sensitization of human T-helper lymphocytes by peptides derived from the V3 loop of human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein.

    abstract::To generate CD4+ T-helper cell lines, lymphocytes from HIV-seronegative subjects were primed in vitro with peptides derived from the V3 loop of HIV-1 gp120. Antigen-specific reactivity was inhibited by an anti-DR monoclonal antibody, indicating HLA-class II dependency, but peptides could be recognized in different HLA...


    pub_type: 杂志文章


    authors: Billaud JN,Yagello M,Gluckman JC

    更新日期:1994-01-01 00:00:00

  • Are immunosuppressive medications associated with decreased responses to routine immunizations? A systematic review.

    abstract:BACKGROUND:Long-term immunosuppressive medications are being used more commonly for a variety of medical conditions, including immune-mediated diseases and organ transplantation. While these medications are often necessary, they are associated with an increased risk of serious infections. Vaccination may be a way to pr...


    pub_type: 杂志文章,评审


    authors: Agarwal N,Ollington K,Kaneshiro M,Frenck R,Melmed GY

    更新日期:2012-02-14 00:00:00

  • Vaccines and the regulatory arm of the immune system. An overview from the Trypanosoma cruzi infection model.

    abstract::The knowledge that the immune system is composed of a regulatory/suppressor arm added a new point of view to better understand the nature of several pathologies including cancer, transplants, infections and autoimmune diseases. The striking discoveries concerning molecules and cells involved in this kind of regulation...


    pub_type: 杂志文章,评审


    authors: Cabrera G,Marcipar I

    更新日期:2019-06-19 00:00:00

  • Co-administration of attenuated Mycoplasma hyopneumoniae 168 strain with bacterial DNA enhances the local and systemic immune response after intranasal vaccination in pigs.

    abstract::Mycoplasma hyopneumoniae, the primary pathogen of enzootic pneumonia, occurs worldwide and causes major economic losses to the pig industry. M. hyopneumoniae infects pigs at mucosal surfaces of respiratory tract. The aim of the present study was to investigate if the protection rate against M. hyopneumoniae infection ...


    pub_type: 杂志文章


    authors: Li Y,Li P,Wang X,Yu Q,Yang Q

    更新日期:2012-03-09 00:00:00

  • Antibody induction after combined application of an adjuvanted recombinant FeLV vaccine and a multivalent modified live virus vaccine with a chlamydial component.

    abstract::The compatibility, safety and interaction on antibody induction of a combined vaccine application were assessed. Specific pathogen-free cats were vaccinated with either a modified live virus vaccine containing feline calici- (FCV), herpes- (FHV-1), parvovirus (FPV) and Chlamydophila felis (C. felis), an adjuvanted rec...


    pub_type: 杂志文章


    authors: Brunner C,Kanellos T,Meli ML,Sutton DJ,Gisler R,Gomes-Keller MA,Hofmann-Lehmann R,Lutz H

    更新日期:2006-03-10 00:00:00

  • Seasonal influenza vaccine efficacy and its determinants in children and non-elderly adults: a systematic review with meta-analyses of controlled trials.

    abstract:CONTEXT:The true level of influenza vaccine efficacy is controversial and many factors may influence its estimation. OBJECTIVES:To estimate the efficacy of vaccination of children and non-elderly adults for the prevention of influenza and to explore the impact of type of vaccine, age, degree of strain matching, influe...


    pub_type: 杂志文章,meta分析,评审


    authors: DiazGranados CA,Denis M,Plotkin S

    更新日期:2012-12-17 00:00:00

  • Long-term effectiveness against cholera of oral killed whole-cell vaccine produced in Vietnam.

    abstract::We assessed the long-term protection afforded by a killed whole-cell oral cholera vaccine produced in Vietnam. A mass immunization of children and adults with the killed whole-cell oral cholera vaccine was undertaken in half of the communes of Hue, Vietnam, in 1998; the remaining communes were immunized in 2000. No ch...


    pub_type: 杂志文章


    authors: Thiem VD,Deen JL,von Seidlein L,Canh DG,Anh DD,Park JK,Ali M,Danovaro-Holliday MC,Son ND,Hoa NT,Holmgren J,Clemens JD

    更新日期:2006-05-15 00:00:00

  • Pediatric immunization-related safety incidents in primary care: A mixed methods analysis of a national database.

    abstract:BACKGROUND:Children are scheduled to receive 18-20 immunizations before their 18th birthday in England and Wales; this approximates to 13 million vaccines administered per annum. Each immunization represents a potential opportunity for immunization-related error and effective immunization is imperative to maintain the ...


    pub_type: 杂志文章


    authors: Rees P,Edwards A,Powell C,Evans HP,Carter B,Hibbert P,Makeham M,Sheikh A,Donaldson L,Carson-Stevens A

    更新日期:2015-07-31 00:00:00

  • Seroprevalence of pertussis among healthcare workers: A cross-sectional study from Tunisia.

    abstract::This cross-sectional study aimed to assess pertussis seroprevalence among healthy healthcare workers (HCW) of the Children's Hospital of Tunis, Tunisia. During the study period, 236 blood samples were obtained to determine HCW exposure to pertussis. Concentrations of immunoglobulin G (IgG) to pertussis toxin (PT) were...


    pub_type: 杂志文章


    authors: Ben Fraj I,Smaoui H,Zghal M,Sassi O,Guiso N,Kechrid A

    更新日期:2019-01-03 00:00:00

  • The adjuvant CLDC increases protection of a herpes simplex type 2 glycoprotein D vaccine in guinea pigs.

    abstract::Herpes simplex virus (HSV) infections are common but there is no vaccine available. We evaluated cationic liposome-DNA complexes (CLDC) as an adjuvant for an HSV gD2 vaccine and compared it to an MPL/Alum adjuvant in a guinea pig model of genital herpes. The addition of CLDC to the gD2 vaccine significantly decreased ...


    pub_type: 杂志文章


    authors: Bernstein DI,Farley N,Bravo FJ,Earwood J,McNeal M,Fairman J,Cardin R

    更新日期:2010-05-07 00:00:00