Abstract:
:The murine cytomegalovirus (MCMV) glycoprotein B (gB) gene was expressed in an adenovirus replication-deficient vector. This virus, designated Ad-gB, was used to immunize BALB/c and B6 mice by the intranasal (i.n.) route to induce an immune response. Following primary immunization, antibody was detected in serum of 100% of vaccinees, as well as the bronchoalveolar lavage, fecal suspensions and vaginal washings. The viral titer of lung and salivary gland of vaccinees 10 days after intranasal challenge with MCMV at 10(5) or 10(3)plaque forming units (PFU) were significantly reduced compared to controls. Re-exposure of vaccinees to Ad-gB 30 days after primary immunization induced a remarkable boost of serum and mucosal antibody responses and further reduction of MCMV titers in the lung and salivary glands. The ability to induce both a systemic and mucosal immune response to a specific gene product may be important in reducing horizontal transmission of CMV infections across mucosal surfaces and in altering host immunity to CMV.
journal_name
Vaccinejournal_title
Vaccineauthors
Shanley JD,Wu CAdoi
10.1016/s0264-410x(03)00037-9subject
Has Abstractpub_date
2003-06-02 00:00:00pages
2632-42issue
19-20eissn
0264-410Xissn
1873-2518pii
S0264410X03000379journal_volume
21pub_type
杂志文章相关文献
VACCINE文献大全abstract::The feasibility of developing a prophylactic vaccine against SARS was assessed by comparing the immune responses elicited by immunizing mice with a recombinant SARS spike glycoprotein (S-protein) formulated with different adjuvants, given by different routes. In both young and aged mice, an intranasal Protollin-formul...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.06.017
更新日期:2007-08-21 00:00:00
abstract::TA-CIN is a vaccine that comprises the human papillomavirus (HPV) type 16 L2, E6 and E7 as a single fusion protein. In a mouse model, TA-CIN effectively prevented outgrowth of HPV16-positive tumour cells. To assess the safety and immunogenicity of TA-CIN, a dose escalating (26, 128, 533 micro g), double blind and plac...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0264-410x(02)00350-x
更新日期:2002-10-04 00:00:00
abstract::ArtinM and Jacalin (JAC) are lectins from the jackfruit (Artocarpus integrifolia) that have important role in modulation of immune responses to pathogens. Neospora caninum is an Apicomplexa parasite that causes neuromuscular disease in dogs and reproductive disorders in cattle, with economic impact on the livestock in...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.09.136
更新日期:2011-11-15 00:00:00
abstract::The costs of developing and producing new-generation vaccines have increased compared to many of the older, "traditional" vaccines because of new technologies and regulatory requirements. While the public sector often supports basic research costs, private manufacturers are usually responsible for the investments in p...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2006.05.042
更新日期:2006-08-31 00:00:00
abstract::Three doses of hepatitis B vaccine were given at 2, 4 and 9 months of age to 220 Senegalese infants living in the Dakar area of Senegal. Half of the infants received 5 micrograms plasma-derived hepatitis B vaccine (Hevac B) and the remainder 20 micrograms mammalian cell-derived recombinant hepatitis B vaccine (GenHeva...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(92)90067-t
更新日期:1992-01-01 00:00:00
abstract::The distribution of alpha-casein, bovine serum albumin (BSA), myoglobin and recombinant protective antigen (rPA) in mono-valent and combination vaccines containing aluminum hydroxide adjuvant was studied by fluorescence microscopy and flow cytometry. Green and red fluorescent probes were conjugated to the antigens. Ad...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2003.10.040
更新日期:2004-05-07 00:00:00
abstract::The use of pneumococcal polysaccharide vaccine (PPV) is low in some countries, maybe because of doubts regarding its efficacy. This meta-analysis aims at combining evidence from randomized trials of PPV assessing its efficacy in preventing Streptococcus pneumoniae related diseases in immunocompetent adults. In the fou...
journal_title:Vaccine
pub_type: 杂志文章,meta分析
doi:10.1016/s0264-410x(01)00217-1
更新日期:2001-09-14 00:00:00
abstract::M2 is the third integral membrane protein of influenza A. M2e, the extracellular, 23 amino acid residues of M2, has been remarkably conserved in all human influenza A strains. This prompted us to evaluate the use of M2e as a potential broad-spectrum immunogen in a mouse model for influenza infection. Genetic fusion of...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.05.082
更新日期:2006-11-10 00:00:00
abstract:INTRODUCTION:Two types of Japanese encephalitis (JE) vaccines, inactivated JE vaccine (JE-I) and live-attenuated JE vaccine (JE-L), are available and used in China. In particular, one JE-L, produced by a domestic manufacturer in China, was prequalified by WHO in 2013. We assessed the safety of JE vaccines in China duri...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.05.021
更新日期:2017-06-22 00:00:00
abstract::We evaluated invasive pneumococcal disease (IPD), antimicrobial resistance and nasopharyngeal colonization before and after introduction of pneumococcal conjugate vaccine (PCV7) in Alaska Natives (AN), a population with high IPD rates. We obtained IPD rates from population-based surveillance. Colonization was determin...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.08.100
更新日期:2005-12-01 00:00:00
abstract::Active surveillance was conducted by the 12 centers of the Canadian Immunization Monitoring Program, Active from 2000-2007 in children 16 years of age and younger to determine the influence of 7-valent pneumococcal conjugate immunization programs on the prevalence, serotype and antibiotic resistance patterns of invasi...
journal_title:Vaccine
pub_type: 杂志文章,多中心研究
doi:10.1016/j.vaccine.2009.12.026
更新日期:2010-02-25 00:00:00
abstract::Because of demonstrable cross-reactivity of cellular antigens contained in B subunit-killed whole-cell (BS-WC) and killed whole-cell-only (WC) oral cholera vaccines with antigens of various non-cholera species of the family Vibrionaceae (NCV), the protection conferred by the vaccines against diarrhoea associated with ...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0264-410x(89)90048-0
更新日期:1989-04-01 00:00:00
abstract::Multiple-antigen peptides (MAPs), containing B- and T-cell epitopes of the Plasmodium falciparum circumsporozoite (CS) protein, have been designed to overcome the limitations of first-generation peptide vaccines caused by low epitope density, carrier toxicity and the lack of parasite-derived T-cell epitopes. The immun...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(94)90337-9
更新日期:1994-08-01 00:00:00
abstract::Since many years Afssaps applies the 3R's strategy (replacement, reduction and refinement) for the use of laboratory animal testing in the framework of vaccine batch release. In this context, for Hepatitis A vaccines, a study was carried out to assess the feasibility of replacing the in vivo "Gold Standard" potency as...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.12.006
更新日期:2010-02-17 00:00:00
abstract::Recombinant canarypox (CP) and fowlpox (FP) viruses that contained two forms of the HIV-1 (SF2 strain) env gene were engineered and their expression analysed in chick, simian and human cells. These vectors can efficiently replicate in avian but not in mammalian cells, in which infection is abortive. The two forms, con...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(94)90180-5
更新日期:1994-09-01 00:00:00
abstract::Thirty-two rhesus monkeys were used to evaluate the dose response of a recombinant HEV vaccine, and the efficacy of the vaccine based on the ORF2 protein of the Pakistani strain for pre- and post-exposure vaccination against intravenous challenge with homologous or heterologous virus was examined. Post-exposure vaccin...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(97)00145-x
更新日期:1997-12-01 00:00:00
abstract:BACKGROUND:Immunization with pneumococcal vaccines is an important prophylactic strategy for children with asplenia or splenic dysfunction, who are at high risk of bacterial infections (including S. pneumoniae). This study aimed to assess immunogenicity and safety of pneumococcal non-typeable Haemophilus influenzae pro...
journal_title:Vaccine
pub_type: 杂志文章,多中心研究
doi:10.1016/j.vaccine.2017.08.039
更新日期:2017-09-25 00:00:00
abstract::A new rabies vaccine was developed from Vero cells adhered to microcarriers, cultivated in a bioreactor in serum-free medium and infected with the PV/VERO-Paris rabies virus strain. The viral suspensions were concentrated by tangential filtration, purified by chromatography and inactivated with beta-propiolactone. In ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.06.014
更新日期:2004-12-09 00:00:00
abstract::Plague, which is initiated by Yersinia pestis infection, is a fatal disease that progresses rapidly and leads to high mortality rates if not treated. Antibiotics are an effective plague therapy, but antibiotic-resistant Y. pestis strains have been reported and therefore alternative countermeasures are needed. In the p...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.07.059
更新日期:2011-09-16 00:00:00
abstract:BACKGROUND:In the Netherlands, different hepatitis B vaccination schedules have been used for children born to HBV-infected mothers. All schedules included a birth dose of hepatitis B immunoglobuline (HBIg). We assessed determinants of perinatal HBV transmission and determinants of anti-HBs titers in infants born to HB...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.12.071
更新日期:2012-02-21 00:00:00
abstract::Serological surveys in many communities show a high prevalence of antibodies to hepatitis A virus (HAV) in people over the age of 50 years. However, few of that age can recall a previous episode of hepatitis, indicating that subclinical infections are common. The outcome of infection with HAV depends on the age at whi...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/0264-410x(92)90544-t
更新日期:1992-01-01 00:00:00
abstract::Few suspension cells can be used for vaccine manufacturing today as they either do not meet requirements from health regulatory authorities or do not produce high virus titres. Two new avian designer cell lines (AGE1.CR and AGE1.CR.pIX) that have been adapted to grow in suspension in serum-free medium were evaluated f...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.05.083
更新日期:2009-08-06 00:00:00
abstract::A post-marketing study was conducted in 26 paediatric practices in Germany to collect safety information on the virosomal adjuvanted influenza vaccine Inflexal V (in Germany marketed as Infectovac Flu). Children aged 6 months to 6 years received one or two doses. Adverse events were documented by parents/legal guardia...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.12.008
更新日期:2009-02-18 00:00:00
abstract:BACKGROUND:Persons with primary immunodeficiency disorders (PIDD) who receive oral poliovirus vaccine (OPV) or are household contacts of OPV recipients are at risk of excreting immunodeficiency-associated vaccine-derived polioviruses (iVDPVs). iVDPVs can be transmitted and cause paralytic polio. The objective of this s...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.06.017
更新日期:2012-08-03 00:00:00
abstract::Herpes simplex virus (HSV) infections are common but there is no vaccine available. We evaluated cationic liposome-DNA complexes (CLDC) as an adjuvant for an HSV gD2 vaccine and compared it to an MPL/Alum adjuvant in a guinea pig model of genital herpes. The addition of CLDC to the gD2 vaccine significantly decreased ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.10.025
更新日期:2010-05-07 00:00:00
abstract::Pneumococcal type 1 pilus proteins have been proposed as potential vaccine candidates. Following conjugate pneumococcal vaccination, the prevalence of the pneumococcal type 1 pilus declined dramatically, a decline associated with the elimination of vaccine-type (VT) strains. Here we show that between 2004 and 2007, th...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.04.042
更新日期:2010-07-05 00:00:00
abstract:BACKGROUND:World Health Organization has recommended the introduction of pneumococcal conjugate vaccine (PCV) in the childhood immunisation programme of all the countries in the world. In lieu of its introduction in India, there is a need to generate evidence on cost-effectiveness of this vaccine. The current study loo...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.12.004
更新日期:2019-01-21 00:00:00
abstract:INTRODUCTION:Replication-competent vaccinia viruses (VACVs) show prolonged antigen expression time and greater stimulation of immune responses than their replication-incompetent counterparts. However, there is the potential risk of serious post-vaccination complications, especially for children and immunocompromised in...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.05.012
更新日期:2016-06-24 00:00:00
abstract:BACKGROUND:Immunization programs currently measure coverage by assessing the proportion of children 12-24 months who have been immunized but this does not address the important question of when the scheduled vaccines were administered. Data capturing the timing of vaccination in first 6 months, when severe disease is m...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2015.12.061
更新日期:2016-02-10 00:00:00
abstract::Hantaviruses cause hemorrhagic fever with renal syndrome (HFRS) in about 150,000 individuals in Eurasia, and several hundred cases of hantavirus pulmonary syndrome (HPS) on the American continent annually. There is consequently a need for rapid diagnostics and effective prevention of hantaviral infections. In this stu...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.09.082
更新日期:2007-02-19 00:00:00