Leukocyte involvement in renal reperfusion-induced liver damage.

Abstract:

BACKGROUNDS/AIMS:Renal ischemia-reperfusion (IR) induces organ damage in remote organs. The aim of this study was to assess the role of leukocytes in the induction of liver damage after renal IR injury. METHODS:Inbred mice were subjected to either sham operation or bilateral renal IR injury (60 min ischemia followed by 3 h reperfusion). Mice were then anesthetized for collection of leukocytes by heart puncture. Isolated leukocytes were transferred to two other groups: intact recipient mice that received leukocytes from IR mice and intact recipient mice that received leukocytes from sham-operated control mice. After 24 h, recipient mice were anesthetized and samples were collected. RESULTS:Alanine aminotransferase, aspartate aminotransferase, and hepatic malondialdehyde increased significantly, and hepatic glutathione decreased significantly in intact recipient mice that received leukocytes from IR mice in comparison with intact recipient mice that received leukocytes from sham-operated control mice. Loss of normal liver architecture, cytoplasmic vacuolization, and focal infiltration of leukocytes were seen. CONCLUSION:These results suggest that leukocytes are one of the possible factors that contribute to liver damage after renal IR injury and this damage is partly due to the induction of oxidative stress.

journal_name

Ren Fail

journal_title

Renal failure

authors

Khastar H,Kadkhodaee M,Sadeghipour HR,Seifi B,Hadjati J,Delavari F,Soleimani M

doi

10.3109/0886022X.2010.541585

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

79-83

issue

1

eissn

0886-022X

issn

1525-6049

journal_volume

33

pub_type

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