Abstract：
UNLABELLED:The pharmacology of progestins includes actions initiated by various cellular targets, including classic receptors characterized as nuclear transcription factors (nPR), G-protein-coupled membrane receptors (mPR), enzymes, membrane channels and transporters. The effects initiated by targets other than nPR are termed non-genomic and there is an increasing recognition that these effects also play an important role in the regulation of cell growth. MATERIALS AND METHODS:The nPR-positive breast cancer (MCF-7) and the nPR-negative uterine cervix cancer (C4-I) cell lines were exposed to progesterone (PG) and mifepristone (MF) during a culture period of 96 h. Daily cell count, cell cycle analysis and apoptosis assay were performed. RESULTS:It was possible to separate the nPR initiated effects (growth stimulation) from the non-genomic effects (growth inhibition) in the MCF-7 cells. Below 1 μM PG treatment gave a small, but distinct increase in cell density which was effectively blocked by MF. Such an effect was absent from the nPR-negative C4-I cells. For a range of concentrations between 1 μM and 100 μM, the effect of both PG and MF developed over time and showed concentration dependency. The PG concentrations needed to reduce cell density by 50% (IC(50)) were 12.8 ± 1.1 μM and 6.5 ± 0.2 μM for the MCF-7 and C4-I cells, respectively. MF appeared to be equally or slightly more potent, with respective IC(50) values of 6.9 ± 0.5 μM and 5.3 ± 0.3 μM. The cell density reduction was both a result of cell cycle arrest and apoptosis. The combination of PG and MF had a potentiated effect on cell density reduction, cell cycle arrest and apoptosis. CONCLUSION:The antiproliferative/cytotoxic effect of PG and MF in concentrations between 1 and 100 μM is of a non-genomic nature.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Fjelldal R,Moe BT,Ørbo A,Sager Gsubject
Has Abstractpub_date
2010-12-01 00:00:00pages
4835-40issue
12eissn
0250-7005issn
1791-7530pii
30/12/4835journal_volume
30pub_type
杂志文章abstract：AIM:To clarify how patients with epidermal growth factor receptor (EGFR)-mutant lung adenocarcinoma with acquired resistance to EGFR-tyrosine kinase inhibitors (TKIs) respond to radiotherapy (RT) for brain metastases. PATIENTS AND METHODS:Forty-seven patients were divided into the following three groups: a TKI-naïve g...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：:The anti-tumor properties of arsenic trioxide have attracted extensive attention after successfully inducing apoptosis of acute promyelocytic leukemia cells. However, the therapeutic spectrum should not only be restricted to acute promyelocytic leukemia, but should also extend into other types of tumor cells. In this ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：:The present study was undertaken to investigate cell death (particularly apoptosis) induced by etoposide, radiation, and both, and to examine p53 protein expression in relation to cell death. Nude mice transplanted with a human tumor (ependymoblastoma) were treated with etoposide (5-40 mg/kg) or 1-2 Gy X-ray irradiati...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:The role of extracellular amines such as noradrenaline and serotonin and their interaction with cyclic nucleotides and intracellular polyamines in the regulation of intestinal epithelial cell proliferation is reviewed with particular reference to the differences between normal and neoplastic cells. In respect to the n...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1987-01-01 00:00:00
abstract：:Chemosensivity testing in 11 retinoblastoma (RB) cell lines identified five tumors showing cross-resistance to plant alkaloids, alkylating agents, anthracycline antibiotics, and antimetabolites, suggestive of the multidrug-resistant (MDR) phenotype described in other tumors. Only two tumors had been clinically exposed...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：AIM:The suitability of Papanicolaou staining and of hematoxylin staining for DNA single-cell cytometry was investigated in comparison to Feulgen staining. MATERIALS AND METHODS:Ten normal cervical smears and ten cervical smears containing cells of a squamous cell carcinoma in situ were analyzed. The integrated optical...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：:Analysis of the overall patterns of gene expression at the polypeptide synthesis level has revealed two S-phase specific, proliferation sensitive nuclear proteins [cyclin (PCNA) and dividin (phosphoprotein)] whose rate of synthesis increases at the G1/S transition border of the cell cycle. All available evidence sugge...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1987-07-01 00:00:00
abstract：SPECIFIC OBJECTIVE:The Epidermal Growth Factor Receptor (EGFR) is a specific cell membrane receptor that shows homology to the product of the oncogene c-erbB2 in human breast cancer. Growth factors bound to the EGFR are able to stimulate the growth of tumor cells in an autocrine or paracrine manner. Our objective was t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：BACKGROUND:The p16 tumour suppressor gene is known to be involved in regulation of the cell cycle. p16 expression in sequential histological stages of oral squamous cell carcinoma (OSCC) formation was investigated using an experimental model of induced oral carcinogenesis in Syrian golden hamsters. MATERIALS AND METHO...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-03-01 00:00:00
abstract：BACKGROUND:Nogo-B was recently shown to be involved in proliferation, apoptosis and invasiveness of cancer cells, whereas its specific receptor (NgBR) was found to be up-regulated in estrogen receptor-α positive breast cancer. No data are currently available concerning their expression in non-small cell lung carcinomas...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-08-01 00:00:00
abstract：BACKGROUND/AIM:Curcumin is a promising nutraceutical for chemoprevention of head and neck squamous cell carcinoma (HNSCC). Capsular formulations of curcumin demonstrate low systemic bioavailability. We aimed to determine if curcumin levels were higher in healthy volunteers and cancer patients with microgranular curcumi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-12-01 00:00:00
abstract：BACKGROUND:Our aim was to analyze the impact of intraperitoneal chemotherapy (IPC), administered with direct peritoneal puncture, on the survival of patients with pretreated ovarian cancer in a real-life setting. PATIENTS AND METHODS:This was a retrospective study comparing patients with advanced ovarian cancer treate...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11256
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND:Genetic alterations occurring in lung cancer are the basis for defining molecular subtypes and essential for targeted therapies. Exhaled breath condensate (EBC) is a form of non-invasive sample that, amongst components, contains DNA from pulmonary tissue. Next-generation sequencing (NGS) was herein used to a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12897
更新日期：2018-10-01 00:00:00
abstract：UNLABELLED:Concurrent chemoradiotherapy has become a standard therapy for locoregionally advanced inoperable nonsmall cell lung cancer (NSCLC). The purpose of this phase II trial was to evaluate the efficacy and toxicity of concurrent chemoradiotherapy following induction with non-platinum chemotherapy in patients with...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-11-01 00:00:00
abstract：:50 patients with advanced breast cancer were treated with the combination of Mitoxantrone 10mg/m2 IV day 2, 5-Fluorouracil 400mg/m2 IV and Cyclophosphamide 300mg/m2 IV day 3, 4, 5, 6 of each monthly cycle. 49 patients are evaluable for toxicity and 47 for efficacy after three months of treatment. Hematologic toxicity ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1987-07-01 00:00:00
abstract：:Serotoninergic system activity and cytokine production have been both implicated in the pathogenesis of cancer anorexia. To verify the existence of relationships between tryptophan, cytokines and anorexia, twenty cancer and six non-neoplastic patients were studied. Plasma amino acid concentration, including tryptophan...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:In order to identify genes associated with metastasis of mammary carcinoma, we compared the transcriptional profile (Affymetrix chip technology) of two cell lines derived from primary mammary carcinoma, three cell lines derived from bone marrow micrometastasis, a cell line derived from a lymph node metastasis as well ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：AIM:To investigate whether there is a difference in selenium levels before and after radiotherapy (RT) and to study the effects of serum selenium levels on RT-related toxicity in patients undergoing RT for head and neck cancer. PATIENTS AND METHODS:A population of 47 consecutive patients was enrolled in the study. RT ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-08-01 00:00:00
abstract：:Therapy of patients with metastatic renal cell carcinoma (mRCC) requires sequential use of several agents with different mechanisms and minimal cross-resistance between the different agents. Tyrosine kinase inhibitors (TKIs) and mammalian target of rapamycin (mTOR) inhibitors prolong progression-free survival (PFS) in...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-10-01 00:00:00
abstract：:This article describes previous studies on a unique protein antitumor antibiotic, neocarzinostatin. Namely, its chemical nature, mode of action at molecular and cellular levels, toxicity and pharmacology, and recent results in clinical trials obtained primarily in Japan have been reviewed briefly. ...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章,评审
doi：
更新日期：1981-01-01 00:00:00
abstract：BACKGROUND:Low 5-fluorouracil (5-FU) concentrations cause a significant increase in DNA synthesis in mitogen-activated human lymphocytes. MATERIALS AND METHODS:We explored 2.5 microM 5-FU-induced DNA synthesis by testing 5-FU activity in hypoxanthine-aminopterin-thymidine (HAT)-containing medium, and its effect on thy...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-10-01 00:00:00
abstract：:Measurement of CA125 is well established in the diagnosis and follow-up of ovarian cancer. CA125 determination is usually performed with the monoclonal antibodies OC125 and M11. For the IMMULITE OM-MA chemoluminsence assay (DPC Biermann, Bad Nauheim, Germany) the monoclonal antibody OV185 is used. We analysed CA125 se...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:The relationship between antioxidant-enzyme defence responses and cellular growth suppression in human MCF-7 (breast) and SW480 (colon) cancer cells, exposed to CLA in culture was studied. MCF-7 and SW480 cells (1 x 10(6)/flask) were cultured in appropriate medium for 4, 8 and 12 days with varying levels of CLA (0-30 ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:In this study, a protein kinase C inhibitor, H-7, was found to potentiate the antiproliferative effects of difluoromethyl ornithine (DFMO), inhibitor of the polyamine biosynthesis, on NIH 3T3 and 3T3/SV40 cells in culture. Incubation of the cells with DFMO inhibited the cell growth, whereas the addition of polyamine s...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-07-01 00:00:00
abstract：BACKGROUND/AIM:A prospective study was designed to investigate the effects of anesthesia, particularly that of the one-lung ventilation procedure (OLV), on the expression of hypoxia-inducible factor 1alpha (HIF1alpha) in patients with lung carcinomas and pneumothorax. MATERIALS AND METHODS:The immunohistochemical expr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-04-01 00:00:00
abstract：BACKGROUND:Gastric cancer is the third most frequent type of neoplasia. In northern Brazil, the State of Pará has a high incidence of this type of neoplasia. Limited data are available so far on the genetic events involved in this disease. MATERIALS AND METHODS:Dual-color fluorescence in situ hybridization (FISH) for ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：:The design of treatment for patients with squamous cell carcinoma of the head and neck (SCCHN) is based on prognostic factors, today essentially performance status and TNM status. It becomes increasingly important to optimise treatment and find the appropriate level of treatment and resource consumption for each patie...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1996-07-01 00:00:00
abstract：:Proteinases are known to be involved in carcinogenesis, and various substrates are now available to measure the activity of these enzymes. No suitable serum tumour marker for head and neck squamous cell carcinoma (HNSCC) exists at this moment. Therefore, we compared proteinase-activity in serum of 20 untreated HNSCC p...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：BACKGROUND:Since specific epidermal growth factor receptor (EGFR) somatic mutations have been demonstrated to influence the response to anti-EGFR therapy in non-small cell lung cancer, EGFR gene mutational analysis of different types of neoplasm is under investigation. PATIENTS AND METHODS:EGFR protein expression and ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
abstract：:MicroRNAs (miRNAs) constitute a dominating class of small RNAs that regulate diverse cellular functions. Due the pivotal role of miRNAs in biological processes, a deregulated miRNA expression is likely involved in human cancers. MicroRNAs possess tumor suppressor capability, as well as display oncogenic characteristic...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.11142
更新日期：2016-11-01 00:00:00
