Abstract:
:The aim of this study was to evaluate a nitrate reductase assay (NRA) for the direct detection of multidrug resistance (MDR) in Mycobacterium tuberculosis from 100 smear-positive sputum samples. The NRA results were compared with the reference proportion method for 100 sputum specimens for which comparable results were available. NRA results were obtained at day 7 for 61 specimens, results for 26 specimens were obtained at day 10, and the results for 13 specimens were obtained at day 14. Thus, 87% of NRA results were obtained in 10 days. NRA is a rapid, accurate, and cost-effective method for the detection of MDR in M. tuberculosis isolates as compared to the proportion method, which is time consuming. Therefore, NRA constitutes a useful tool for detection of tuberculosis drug resistance in low-resource countries with limited laboratory facilities due to its low-cost, ease of performance and lack of requirement of sophisticated equipment.
journal_name
Indian J Med Microbioljournal_title
Indian journal of medical microbiologyauthors
Gupta M,Shamma.,Singh NP,Kaur IRdoi
10.4103/0255-0857.71816subject
Has Abstractpub_date
2010-10-01 00:00:00pages
363-5issue
4eissn
0255-0857issn
1998-3646pii
IndianJMedMicrobiol_2010_28_4_363_71816journal_volume
28pub_type
杂志文章abstract::The aim of this study was to explore baseline data, laboratory and molecular analyses to determine if any could serve as potential prognostic marker(s) for treatment response to second line tuberculosis regimens. Of a total number of 50 multi-drug resistant tuberculosis (MDR-TB) patients starting second-line drug MDR-...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.118899
更新日期:2013-10-01 00:00:00
abstract:PURPOSE:Opportunistic viral infections cause increased morbidity and mortality among human immunodeficiency virus (HIV) infected individuals, especially those who are not on antiretroviral treatment. Early diagnosis of these opportunistic viruses will be able to reduce the risk of disease progression with appropriate i...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.53202
更新日期:2009-07-01 00:00:00
abstract:PURPOSE:The aim of this study was to isolate a novel mycobacteriophage and then explore its anti-tuberculosis (TB) potential. MATERIALS AND METHODS:Phage was isolated from enriched soil sample. A total of 36 mycobacterial strains obtained from clinical specimens were subjected to investigate the host range of phage by...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.180282
更新日期:2016-04-01 00:00:00
abstract::An attempt was made to speciate 102 clinically significant isolates of coagulase negative staphylococci (CoNS) by a practical scheme adapted from various references. This scheme utilizes slide and tube coagulase test, urease test ornithine decarboxylase, novobiocin susceptibility and aerobic acid from mannose for assi...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract:PURPOSE:To evaluate the utility of the polymerase chain reaction (PCR) test for diagnosing osteoarticular tuberculosis (TB). METHODS:Clinical samples (synovial tissue and synovial fluid) obtained from 23 cases of suspected osteoarticular tuberculosis were subjected to Ziehl Neelsen (ZN) smear examination, radiometric ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2005-10-01 00:00:00
abstract::[This corrects the article DOI: 10.4103/0255-0857.188314.]. ...
journal_title:Indian journal of medical microbiology
pub_type: 已发布勘误
doi:10.4103/0255-0857.195384
更新日期:2016-10-01 00:00:00
abstract::Invasive fungal infections (IFIs) are an important cause of morbidity and mortality in paediatric leukaemias. Antifungal combinations to treat these patients are being explored. Fourteen children with leukaemias and IFIs were treated with a combination of antifungal agents at our centre. The first antifungal was ampho...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_18_157
更新日期:2019-01-01 00:00:00
abstract::Emergence of extended spectrum beta lactamases (ESBLs) producing strains of gram negative bacteria, as one of the leading cause of septicaemia often complicates the clinical and therapeutic outcome. The present study was undertaken to investigate the prevalence of ESBLs in bacteria isolated from neonatal septicaemic c...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.43578
更新日期:2008-10-01 00:00:00
abstract::Histopathological examination of tissue biopsies for the identification of infectious organisms is a very important diagnostic tool. Conventional culture confirmation of tissue biopsies often fail to identify any pathogen as, first of all, invariably most of the tissue samples that are collected and sent for culture i...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.4103/0255-0857.49423
更新日期:2009-04-01 00:00:00
abstract::Mycobacterial diseases continue to cause high morbidity and mortality. Isolation, identification and sensitivity testing form the backbone of laboratory investigations. M. tuberculosis isolation needs 6-8 weeks on conventional egg containing media. For rapid isolation various methods have been evaluated. We evaluated ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.16050
更新日期:2005-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Hepatitis C is global health problem affecting a significant portion of the world's population. Available data in Western Maharashtra on seroprevalence, risk factors and genotype distribution are very limited. OBJECTIVES:The present study was carried out to estimate the seroprevalence, factor...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_16_96
更新日期:2017-10-01 00:00:00
abstract:PURPOSE:The main purpose of this study was to establish 'Antimicrobial Resistance Surveillance Network' in India and to monitor the antimicrobial susceptibility profile of clinical isolates to establish a national network across the country for monitoring antimicrobial resistance in Salmonella. MATERIALS AND METHODS:T...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,多中心研究
doi:10.4103/ijmm.IJMM_16_382
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:The purpose of this study was to ascertain if the simple practice of Gram stain, acridine orange stain and direct sensitivity determination of positive blood culture bottles could be used to guide early and appropriate treatment in trauma patients with clinical suspicion of sepsis. The study also aimed to evalu...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.62491
更新日期:2010-04-01 00:00:00
abstract:Background:Raoultella is a Gram-negative bacteria, which commonly occur in the natural environment such as water, soil and on plants. In recent years, Raoultella spp. gained more interest. There is also an increasing number of publications describing mainly clinical cases involving these bacteria. Identification of Rao...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_17_99
更新日期:2018-04-01 00:00:00
abstract::Aspergilloma is a fungal ball that usually forms in a preformed stationary cavity, mostly in lung and paranasal sinuses. We report a rare case of primary orbital Aspergilloma following exenteration for an invasive ocular surface squamous neoplasia, clinically mimicking a recurrence of the tumor. The fungal ball showed...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::Hemophagocytic lymphohistiocytosis (HLH) triggered by Parvovirus B19 and Epstein-Barr virus co-infection is rare and unknown in infants. A 2-month-old male infant with fever, rash, bicytopenia and hepato-splenomegaly died owing to diagnostic dilemmas. Hence simply testing for hyperferritinaemia and hypertriglyceridemi...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.129819
更新日期:2014-04-01 00:00:00
abstract:PURPOSE:Ofloxacin (OFX) is one of the potent fluoroquinolone (FQ) recommended to treat MDR-TB. Over a decade, the preexposure of this drug for the treatment of other bacterial infections has resulted in acquisition of FQ resistance among Mycobacterium tuberculosis strains. Considering this possibility, a study was unde...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.83913
更新日期:2011-07-01 00:00:00
abstract::We report here the emergence of a new focus of cutaneous leishmaniasis (CL) due to Leishmania tropica (L. tropica) in the Ajmer city of Rajasthan, India, a previously non-endemic area. Between January-February 2006, 13 new indigenously acquired cases of CL were diagnosed among the patients attending the Skin and STD d...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.34774
更新日期:2007-07-01 00:00:00
abstract::Rhodococcus equi , previously known as Corynebacterium equi, is one of the most important causes of zoonotic infection in grazing animals. Increased cases of human infection with R. equi have been reported especially in immunocompromised patients. Infection in immunocompetent patients is extremely rare. We report a ca...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.76529
更新日期:2011-01-01 00:00:00
abstract::Outbreaks of varicella are reported when susceptible population accumulates. This study reports a chickenpox outbreak in Burail in August 2014, wherein 20 laboratory-confirmed cases were identified by the detection of varicella zoster virus (VZV) DNA and VZV IgM antibodies. The viral load between vesicular swabs and s...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_16_420
更新日期:2017-07-01 00:00:00
abstract::Beta-hemolytic Enterococcus faecalis was isolated from the pericardial fluid obtained from a patient with pyopericardium. The patient was immunocompetent and had mild pleural effusion. He was treated with parenteral co-amoxiclav and amikacin, had underwent pericardiectomy with repeated pericardial aspiration, and reco...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.38870
更新日期:2008-01-01 00:00:00
abstract:PURPOSE:The objective of this study is to determine the role of quantitative cultures of non-bronchoscopic samples such as blinded bronchial sampling (BBS) and endotracheal aspirates (ETA) in the management of ventilator associated pneumonia (VAP). The study also evaluates the clinical diagnosis of VAP based on the inc...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.25226
更新日期:2006-04-01 00:00:00
abstract::A male infant admitted with pyogenic meningitis with protein energy malnutrition developed fatal infection due to Salmonella enteritidis. The same organism was isolated from CSF and blood cultures. ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2001-07-01 00:00:00
abstract::Over a five year period, stool samples were screened for Vibrionaceae from cases of acute diarrhoea, to study their isolation rate and their antimicrobial sensitivity pattern. All the isolates were identified by standard laboratory techniques. A total of 323 species belonging to Vibrionaceae were isolated from 4492 st...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00
abstract::Ocular infection with microsporidia has been documented in both immunocompetent and immunocompromised patients. Sources and mode of human infection with microsporidia have been difficult to ascertain although exposure to water may be an important risk factor. Of four genera that have been reported in human disease, on...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.90189
更新日期:2011-10-01 00:00:00
abstract::Extended spectrum -lactamases (ESBLs) are plasmid mediated, TEM and SHV derived enzymes, first isolated in Western Europe in mid 1980s, most commonly in Klebsiella spp., followed by Escherichia coli. These enzymes are capable of hydrolyzing broad spectrum cephalosporins and monobactams but inactive against cephamycins...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2004-04-01 00:00:00
abstract:PURPOSE:To describe the changes in antibiotic susceptibility patterns of common intensive care unit pathogens with time from the medical intensive care unit of a tertiary care hospital. METHODS:A prospective observational study was conducted in the medical intensive care unit (MICU) of a 2100 bed tertiary care hospita...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.34764
更新日期:2007-07-01 00:00:00
abstract:Purpose:The Revised National Tuberculosis Control Programme (RNTCP) is now introducing daily fixed-dose regimen instead of Directly Observed Treatment, Short Course (DOTS) regimen for treatment of drug-sensitive tuberculosis (TB) in India. It would be beneficial to understand the drawbacks, barriers and advantages of t...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_18_65
更新日期:2018-04-01 00:00:00
abstract::Brevibacteria are a part of the normal skin flora and may be dismissed in blood cultures as contaminants. They have been reported as opportunistic pathogens in immunocompromised patients. We report a catheter-related bloodstream infection with Brevibacterium casei in a 6-year-old child with aplastic anaemia. Treatment...
journal_title:Indian journal of medical microbiology
pub_type:
doi:10.4103/ijmm.IJMM_20_292
更新日期:2020-04-01 00:00:00
abstract:BACKGROUND:Pneumonia is a preventable cause of mortality in children. Streptococcus pneumoniae colonising the nasopharynx of healthy children can cause invasive diseases and the serotype distribution of colonisation isolates should be an indicator of invasive disease, antibiotic resistance profiles, and potential vacci...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_15_347
更新日期:2017-10-01 00:00:00