Histopathology for the diagnosis of infectious diseases.

Abstract:

:Histopathological examination of tissue biopsies for the identification of infectious organisms is a very important diagnostic tool. Conventional culture confirmation of tissue biopsies often fail to identify any pathogen as, first of all, invariably most of the tissue samples that are collected and sent for culture isolation are inappropriately collected in formalin, which prevents pathogen growth in culture media. Inadequate processing like grinding, etc. further hinders isolation. Presence of inhibitors like dead tissue debris, fibers, etc. also delays isolation. Microbiologists often lack expertise in identifying infectious pathogens directly from tissue biopsies by microscopic visualization. This review therefore acquaints microbiologists with the various methods available for detecting infectious agents by using histological stains. On histopathological examination of the tissue biopsy once, it is determined that a disease is likely to be due to an infection and has characterized the inflammatory response and hence associated microorganisms should be thoroughly looked for. Although some microorganisms or their cytopathic effects may be clearly visible on routine haematoxylin- and eosin-stained sections, additional histochemical stains are often needed for their complete characterization. Highly specific molecular techniques, such as immunohistochemistry, in situ hybridization and nucleic acid amplification, may be needed in certain instances to establish the diagnosis of infection. Through appropriate morphologic diagnoses and interlaboratory communication and collaboration, direct microscopic visualization of tissue samples can thus be very helpful in reaching a correct and rapid diagnosis.

journal_name

Indian J Med Microbiol

authors

Gupta E,Bhalla P,Khurana N,Singh T

doi

10.4103/0255-0857.49423

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

100-6

issue

2

eissn

0255-0857

issn

1998-3646

pii

IndianJMedMicrobiol_2009_27_2_100_49423

journal_volume

27

pub_type

杂志文章,评审
  • Injection site abscess due to Mycobacterium fortuitum: a case report.

    abstract::Injection abscess is an iatrogenic infection occurring as an isolated case or as cluster outbreak. These infections occur due to contaminated injectables or lapse in sterilisation protocol. While pathogens such as Pseudomonas, Klebsiella, E. coli, and S. aureus are the usual causative agents, unusual organisms such as...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Devi DR,Indumathi VA,Indira S,Babu PR,Sridharan D,Belwadi MR

    更新日期:2003-04-01 00:00:00

  • ESBL - from petri dish to the patient.

    abstract::Treatment of extended spectrum beta-lactamase (ESBL) producing strains of Enterobacteriaceae has emerged as a major challenge in hospitalised as well as community based patients. Infections due to ESBL producers range from uncomplicated urinary tract infection to life threatening sepsis. Although several reviews have ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.4103/0255-0857.19889

    authors: Bhattacharya S

    更新日期:2006-01-01 00:00:00

  • Investigation of an outbreak of varicella in Chandigarh, North India, using a real-time polymerase chain reaction approach.

    abstract::Outbreaks of varicella are reported when susceptible population accumulates. This study reports a chickenpox outbreak in Burail in August 2014, wherein 20 laboratory-confirmed cases were identified by the detection of varicella zoster virus (VZV) DNA and VZV IgM antibodies. The viral load between vesicular swabs and s...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_16_420

    authors: Singh MP,Kaur R,Kumar A,Gupta M,Garg S,Ratho RK

    更新日期:2017-07-01 00:00:00

  • Identification of opportunistic enteric parasites among immunocompetent patients with diarrhoea from Northern India and genetic characterisation of Cryptosporidium and Microsporidia.

    abstract:PURPOSE:Enteric parasitic infestation is a major public health problem in developing countries. Parasites such as Cryptosporidium spp., Cyclospora spp., Cystoisospora spp. and Microsporidia may cause severe diarrhoea among immunocompromised patients. There is scanty data on their frequency among immunocompetent patient...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.174114

    authors: Ghoshal U,Dey A,Ranjan P,Khanduja S,Agarwal V,Ghoshal UC

    更新日期:2016-01-01 00:00:00

  • Elizabethkingia meningoseptica: an emerging pathogen causing meningitis in a hospitalized adult trauma patient.

    abstract::A 23-year-old male patient who was a follow-up case of neurosurgery presented to our emergency department with a history of high-grade fever and clinical features of meningitis for 1 week. The cerebrospinal fluid (CSF) was sent to our laboratory for culture. The culture demonstrated growth of 1-2 mm in diameter light ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.115653

    authors: Tak V,Mathur P,Varghese P,Misra MC

    更新日期:2013-07-01 00:00:00

  • Staphylococcus aureus colonisation in HIV-infected patients: Incidence, risk factors and subsequent skin- and soft-tissue infections.

    abstract::We evaluated the incidence and risk factors of Staphylococcus aureus colonisation in 300 treatment-naïve HIV patients. Swabs from anterior nares and pharynx were cultured. Eighty-eight patients (29.3%) were colonised with S. aureus (47.7% nasal, 23.8% pharyngeal and 28.5% at both sites), which yielded 112 isolates. Me...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_20_5

    authors: Panigrahy A,Sinha S,Das BK,Kapil A,Vishnubhatla S,Dhawan B

    更新日期:2020-07-01 00:00:00

  • Emergence and dissemination of antibiotic resistance: a global problem.

    abstract::Antibiotic resistance is a major problem in clinical health settings. Interestingly the origin of many of antibiotic resistance mechanisms can be traced back to non-pathogenic environmental organisms. Important factors leading to the emergence and spread of antibiotic resistance include absence of regulation in the us...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.4103/0255-0857.103756

    authors: Choudhury R,Panda S,Singh DV

    更新日期:2012-10-01 00:00:00

  • Combination antifungals as an effective means of salvage in paediatric leukaemia patients with invasive fungal infections.

    abstract::Invasive fungal infections (IFIs) are an important cause of morbidity and mortality in paediatric leukaemias. Antifungal combinations to treat these patients are being explored. Fourteen children with leukaemias and IFIs were treated with a combination of antifungal agents at our centre. The first antifungal was ampho...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_18_157

    authors: Meena JP,Gupta AK,Jana M,Seth R

    更新日期:2019-01-01 00:00:00

  • Neonatal listeriosis followed by nosocomial infection.

    abstract::Neonatal listeriosis is widely reported, but this is the first case reported in Serbia. A newborn developed respiratory distress syndrome 2 hours after delivery and was admitted to the neonatal intensive care unit. Initial empirical therapy was inappropriate. Consequently, on the second day, the patient developed meni...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.115229

    authors: Dinic M,Stankovic S

    更新日期:2013-04-01 00:00:00

  • Spoligotype patterns of Mycobacterium tuberculosis isolated from extra pulmonary tuberculosis patients in Puducherry, India.

    abstract:PURPOSE:Genotyping studies like spoligotyping are valuable tools in understanding the genetic diversity and epidemiology of Mycobacterium tuberculosis. Though there are reports of spoligotyping of M. tuberculosis isolates from pulmonary specimens from different parts of India, spoligotyping of extra pulmonary tuberculo...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.154871

    authors: Kandhakumari G,Stephen S,Sivakumar S,Narayanan S

    更新日期:2015-04-01 00:00:00

  • Discriminatory power of three typing techniques in determining relatedness of nosocomial Klebsiella pneumoniae isolates from a tertiary hospital in India.

    abstract:PURPOSE:The purpose of this study was to evaluate the discriminatory power of two DNA-based technique and a protein-based technique for the typing of nosocomial isolates of Klebsiella pneumoniae. A second objective was to determine the antimicrobial susceptibility pattern and characterise the presence of genes encoding...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_16_308

    authors: Purighalla S,Esakimuthu S,Reddy M,Varghese GK,Richard VS,Sambandamurthy VK

    更新日期:2017-07-01 00:00:00

  • One year trends in the gram-negative bacterial antibiotic susceptibility patterns in a medical intensive care unit in South India.

    abstract:PURPOSE:To describe the changes in antibiotic susceptibility patterns of common intensive care unit pathogens with time from the medical intensive care unit of a tertiary care hospital. METHODS:A prospective observational study was conducted in the medical intensive care unit (MICU) of a 2100 bed tertiary care hospita...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.34764

    authors: Kaul S,Brahmadathan KN,Jagannati M,Sudarsanam TD,Pitchamuthu K,Abraham OC,John G

    更新日期:2007-07-01 00:00:00

  • The use of E-test for the drug susceptibility testing of Mycobacterium tuberculosis - a solution or an illusion?

    abstract:AIM:To evaluate E-test as a tool for rapid determination of drug susceptibility against the conventional LJ method focusing on reliability, expense, ease of standardization and performance of the technique in low resource settings. MATERIALS AND METHODS:A total of 74 clinical isolates (2004-2005) of Mycobacterium tube...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.58725

    authors: Verma JS,Rawat D,Hasan A,Capoor MR,Gupta K,Deb M,Aggarwal P,Nair D

    更新日期:2010-01-01 00:00:00

  • Effectiveness of hand hygiene promotional program based on the WHO multimodal hand hygiene improvement strategy, in terms of compliance and decontamination efficacy in an indian tertiary level neonatal surgical intensive care unit.

    abstract:Background:The WHO Multimodal Hand Hygiene Improvement Strategy (MHHIS) has been proposed to improve the Hand Hygiene (HH) compliance of the WHO recommendations on HH.Therefore, the current study was planned in our neonatal unit with the objective of evaluating the effectiveness of a Hand Hygiene Promotional Program (H...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_20_47

    authors: Thomas AM,Kaur S,Biswal M,N Rao KL,Vig S

    更新日期:2019-10-01 00:00:00

  • Prosthetic joint infection due to Lysobacter thermophilus diagnosed by 16S rRNA gene sequencing.

    abstract::We report the first case of prosthetic joint infection caused by Lysobacter thermophilus which was identified by 16S rRNA gene sequencing. Removal of prosthesis followed by antibiotic treatment resulted in good clinical outcome. This case illustrates the use of molecular diagnostics to detect uncommon organisms in sus...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.174124

    authors: Dhawan B,Sebastian S,Malhotra R,Kapil A,Gautam D

    更新日期:2016-01-01 00:00:00

  • Isolation of Salmonella typhi from high vaginal swab in a case of septic abortion.

    abstract::Salmonella Typhi can be a significant cause of morbidity and mortality in pregnant females with adverse outcomes. Risk of infections increases manifold during pregnancy due to hormonal changes and immunological phenomena. S. Typhi has the ability to cross placenta (vertical transmission) resulting in miscarriage, stil...

    journal_title:Indian journal of medical microbiology

    pub_type:

    doi:10.4103/ijmm.IJMM_16_180

    authors: Jena PP,Duggal SD,Kumar A,Bharara T,Sharma A,Gur R

    更新日期:2017-04-01 00:00:00

  • The antibiotics of choice for the treatment of melioidosis in Indian set up.

    abstract::Therapeutic options for the treatment of melioidosis caused by Burkholderia pseudomallei are limited due to the inherent resistance conferred by this pathogen to various groups of antibiotics. Witnessing an increase in the number of microbiological culture-confirmed cases of melioidosis at our settings in the past few...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.188340

    authors: Shaw T,Tellapragada C,Eshwara VK,Bhat HV,Mukhopadhyay C

    更新日期:2016-07-01 00:00:00

  • Assessment of microbiological status after successful completion of intermittent revised national tuberculosis control programme directly observed treatment, short course regimen for microbiologically confirmed pulmonary tuberculosis cases: While new dail

    abstract:Purpose:The Revised National Tuberculosis Control Programme (RNTCP) is now introducing daily fixed-dose regimen instead of Directly Observed Treatment, Short Course (DOTS) regimen for treatment of drug-sensitive tuberculosis (TB) in India. It would be beneficial to understand the drawbacks, barriers and advantages of t...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_18_65

    authors: Ghosh R,Roy S,Rashid MK

    更新日期:2018-04-01 00:00:00

  • Evaluation of biphasic culture system for mycobacterial isolation from the sputum of patients with pulmonary tuberculosis.

    abstract::Mycobacterial diseases continue to cause high morbidity and mortality. Isolation, identification and sensitivity testing form the backbone of laboratory investigations. M. tuberculosis isolation needs 6-8 weeks on conventional egg containing media. For rapid isolation various methods have been evaluated. We evaluated ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.16050

    authors: Ghatole M,Sable C,Kamale P,Kandle S,Jahagirdar V,Yemul V

    更新日期:2005-04-01 00:00:00

  • Pyopericardium due to group D streptococcus.

    abstract::Beta-hemolytic Enterococcus faecalis was isolated from the pericardial fluid obtained from a patient with pyopericardium. The patient was immunocompetent and had mild pleural effusion. He was treated with parenteral co-amoxiclav and amikacin, had underwent pericardiectomy with repeated pericardial aspiration, and reco...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.38870

    authors: Karthikeyan K,Rajesh KR,Poornima H,Bharathidasan R,Brahmadathan KN,Priyadharsini RI

    更新日期:2008-01-01 00:00:00

  • A case of tuberculous pericardial effusion.

    abstract::Tuberculosis accounts for up to 4% of acute pericarditis and 7% cases of cardiac tamponade. Prompt treatment can be life saving but requires accurate diagnosis. We report a case of 30-year-old male who presented with fever, chills, and dry nonproductive cough since one month. The case was diagnosed by radiological fin...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Wanjari K,Baradkar V,Mathur M,Kumar S

    更新日期:2009-01-01 00:00:00

  • Superantigens - concepts, clinical disease and therapy.

    abstract::The term superantigen was introduced in microbiology and immunology only a decade ago. The unique feature of superantigen is that it bypasses the antigen processing mechanism and specifically binds to TCR v segment and forms a trimolecular complex along with major histocompatibility complex class II. Since its discove...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Hemalatha V,Srikanth P,Mallika M

    更新日期:2004-10-01 00:00:00

  • Blood culture gram stain, acridine orange stain and direct sensitivity-based antimicrobial therapy of bloodstream infection in patients with trauma.

    abstract:PURPOSE:The purpose of this study was to ascertain if the simple practice of Gram stain, acridine orange stain and direct sensitivity determination of positive blood culture bottles could be used to guide early and appropriate treatment in trauma patients with clinical suspicion of sepsis. The study also aimed to evalu...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.62491

    authors: Behera B,Mathur P,Gupta B

    更新日期:2010-04-01 00:00:00

  • Fatal haemophagocytic syndrome and hepatitis associated with visceral leishmaniasis.

    abstract::Haemophagocytic syndrome (HPS) secondary to infections occurs due to excessive, non-malignant proliferation of histiocytes, with resultant haemophagocytosis. The syndrome is essentially treatable, provided timely etiological diagnosis is achieved. In this report, we present a rare case of a child who hailed from Uttar...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.37354

    authors: Mathur P,Samantaray JC,Samanta P

    更新日期:2007-10-01 00:00:00

  • Fusarium: The versatile pathogen.

    abstract::Fusarium is an emerging human opportunistic pathogen of growing importance, especially among immunosuppressed haematology patients due to an increased incidence of disseminated infections over the past two decades. This trend is expected only to continue due to the advances in medical and surgical technologies that wi...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.4103/ijmm.IJMM_16_24

    authors: Tupaki-Sreepurna A,Kindo AJ

    更新日期:2018-01-01 00:00:00

  • Necrotizing (malignant) otitis externa: an unusual localization of mucormycosis.

    abstract::Malignant otitis externa (MOE) is a severe infection of external auditory canal and skull base. A 17-year-old diabetic girl was admitted with diabetic ketoacidosis. Cellulitis of her right ear occurred on the second day of hospitalization and a black necrotic scar in the same region appeared on the next day. The lesio...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.29390

    authors: Tuzcu A,Bahceci M,Celen MK,Kilinc N,Ozmen S

    更新日期:2006-10-01 00:00:00

  • Hepatitis C virus and its genotypes in chronic liver disease patients from Meghalaya, Northeast India.

    abstract:Background and Objectives:Hepatitis C virus (HCV) is an important cause of chronic liver disease (CLD). Although Northeast India is believed to be a HCV hotspot, the proportion of HCV infection and the distribution of HCV genotypes in CLD cases from the region are not known. The objectives of the study were to determin...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_17_371

    authors: Barman B,Bora K,Lynrah KG,Lyngdoh WV,Jamil M

    更新日期:2018-07-01 00:00:00

  • Detection of plasmid-mediated AmpC β-lactamase in Escherichia coli and Klebsiella pneumoniae.

    abstract:BACKGROUND:Detecting plasmid-mediated AmpC (pAmpC) β-lactamase-producing organism is important for optimal infection control and providing accurate and effective treatment option for physicians. OBJECTIVES:The aim of this study was to investigate the prevalence of pAmpC β-lactamase and compare the results of boronic a...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.108723

    authors: Yilmaz NO,Agus N,Bozcal E,Oner O,Uzel A

    更新日期:2013-01-01 00:00:00

  • Occurrence of II and V staphylococcal cassette chromosome mec types among coagulase-negative staphylococci from Northeastern part of India.

    abstract:Introduction:Coagulase Negative Staphylococci, the most commonly isolated pathogen are becoming emerging threats to the community as well as to the nosocomial environment. The present study underscores the distribution of Staphylococcal cassette chromosome mec (SCCmec) types among Methicillin resistant Coagulase Negati...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_18_285

    authors: Bhowmik D,Chakraborty P,Bhowmick R,Sarkar R,Chetri S,Bhattacharjee A

    更新日期:2018-10-01 00:00:00

  • An evaluation of four different phenotypic techniques for detection of metallo-beta-lactamase producing Pseudomonas aeruginosa.

    abstract:PURPOSE:The present study was undertaken to detect metallo-beta-lactamase (MBL) in nosocomial isolates of Pseudomonas aeruginosa by four different phenotypic methods. METHODS:Ninety-one consecutive P. aeruginosa isolates were subjected to susceptibility testing by disc-diffusion assay and Vitek 2. Imipenem resistance ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.39587

    authors: Behera B,Mathur P,Das A,Kapil A,Sharma V

    更新日期:2008-07-01 00:00:00