Abstract:
Background:Raoultella is a Gram-negative bacteria, which commonly occur in the natural environment such as water, soil and on plants. In recent years, Raoultella spp. gained more interest. There is also an increasing number of publications describing mainly clinical cases involving these bacteria. Identification of Raoultella spp. is difficult due to a phylogenetic relationship with Klebsiella spp. Purpose:Available biochemical tests do not always allow for their identification to species. Thus, the aim of this study was to evaluate selected methods of identification of Raoultella spp. and their differentiation from genus Klebsiella. Materials and Methods:In this evaluation three methods were used such as manual test ID32E (bioMérieux), automatic test VITEK2 Compact (bioMérieux) and matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) method (Bruker). Results:Good identification of the species was obtained for 81.4% of the strains in the ID32E test, 93.3% in VITEK2 Compact test, and 97.4% in MALDI-TOF MS method, respectively. Conclusion:It was established that MALDI-TOF MS method is reliable in identifying genus Raoultella.
journal_name
Indian J Med Microbioljournal_title
Indian journal of medical microbiologyauthors
Sekowska A,Mikucka A,Gospodarek-Komkowska Edoi
10.4103/ijmm.IJMM_17_99subject
Has Abstractpub_date
2018-04-01 00:00:00pages
197-200issue
2eissn
0255-0857issn
1998-3646pii
IndianJMedMicrobiol_2018_36_2_197_238682journal_volume
36pub_type
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