Abstract:
PURPOSE:The Western Blot test is considered a gold standard test for the confirmation of an ELISA and/or rapid assay screened reactive sample in the diagnosis of HIV infection, especially in the low risk population. In this study, an indigenously developed HIV W. Blot kit (J.Mitra & Co., New Delhi, India) was compared for its performance characteristics with a widely used Western Blot kit, HIV Blot 2.2 (Genelabs, Singapore). Antigens of both HIV-1 and the indicator antigen gp36 of HIV-2 are included in the strips. METHODS:A panel of 150 clinical serum samples was used in the evaluation. All the sera were tested simultaneously by both the kits. RESULTS:The HIV W. Blot kit had high performance characteristics (100% sensitivity and 100% specificity), like the HIV Blot 2.2. The test procedure was easy to perform. There was clear delineation of the bands. CONCLUSIONS:The interpretation of the results on the HIV W. Blot was less prone to subjective errors. The test gave positive bands at even very high serum dilutions in the test kit. This fact indicates that HIV W. Blot probably has a potential application in early phases of infection, when the antibody concentrations are still very low.
journal_name
Indian J Med Microbioljournal_title
Indian journal of medical microbiologyauthors
Lakshmi V,Ponamgi SPsubject
Has Abstractpub_date
2002-10-01 00:00:00pages
200-5issue
4eissn
0255-0857issn
1998-3646journal_volume
20pub_type
杂志文章abstract::Extended spectrum -lactamases (ESBLs) are plasmid mediated, TEM and SHV derived enzymes, first isolated in Western Europe in mid 1980s, most commonly in Klebsiella spp., followed by Escherichia coli. These enzymes are capable of hydrolyzing broad spectrum cephalosporins and monobactams but inactive against cephamycins...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2004-04-01 00:00:00
abstract:PURPOSE:To find out the most suitable serological investigative procedures to diagnose dengue cases effectively in the laboratory practice identifying primary and secondary cases as well as period of suffering. MATERIALS AND METHODS:Dengue suspected cases sent to the laboratory in 2012 in central Kolkata by the local ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.136563
更新日期:2014-07-01 00:00:00
abstract:AIM:To evaluate E-test as a tool for rapid determination of drug susceptibility against the conventional LJ method focusing on reliability, expense, ease of standardization and performance of the technique in low resource settings. MATERIALS AND METHODS:A total of 74 clinical isolates (2004-2005) of Mycobacterium tube...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.58725
更新日期:2010-01-01 00:00:00
abstract::Gut microbiota are microorganisms that inhabit the gut; they coexist peacefully with the host, thereby contributing to the health and well-being of individuals. Bacteroidetes and Firmicutes largely dominate the gut microbial flora. The intestinal flora promotes intestinal mucosal integrity, provides essential nutrient...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.4103/ijmm.IJMM_19_373
更新日期:2019-04-01 00:00:00
abstract::Paragonimiasis is a foodborne parasitic zoonosis caused by lung fluke species of the genus Paragonimus. The Paragonimus westermani is the most common human pathogen in Asian countries. In northeast India, Paragonimus heterotremus has been documented as the only human pathogen in the earlier literature. In India, P. we...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.150950
更新日期:2015-02-01 00:00:00
abstract:INTRODUCTION:Recent years have seen a rise of coagulase-negative staphylococci (CoNS) from common contaminants to agents of nosocomial blood stream infections (BSI's). Molecular typing and establishing a correlation with antibiotic resistance is essential particularly in countries like India where genotyping studies fo...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.195374
更新日期:2016-10-01 00:00:00
abstract::[This corrects the article DOI: 10.4103/0255-0857.188314.]. ...
journal_title:Indian journal of medical microbiology
pub_type: 已发布勘误
doi:10.4103/0255-0857.195384
更新日期:2016-10-01 00:00:00
abstract::Corynebacterium striatum is an emerging nosocomial pathogen associated with wound infections, pneumonia and meningitis. It is also a multidrug-resistant pathogen causing high morbidity. This is a report of an unusual case of wound infection in a patient with laryngeal carcinoma. Accurate diagnosis of the infection and...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.136589
更新日期:2014-07-01 00:00:00
abstract::Antibiotic resistance is a major problem in clinical health settings. Interestingly the origin of many of antibiotic resistance mechanisms can be traced back to non-pathogenic environmental organisms. Important factors leading to the emergence and spread of antibiotic resistance include absence of regulation in the us...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.4103/0255-0857.103756
更新日期:2012-10-01 00:00:00
abstract::Ocular nocardiosis is an opportunistic infection and is believed to be a rare entity. We describe a rare case report of a patient with culture-positive Nocardia asteroides canaliculitis who presented with complaints of watering, purulent discharge and painful swelling of left lower eye lid. A purulent tenacious materi...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2004-04-01 00:00:00
abstract:PURPOSE:To determine the risk factors, microbiological features, clinical features and other epidemiological characteristics of Nocardia keratitis seen at a tertiary eye care centre in south India. METHODS:We evaluated 31 patients with Nocardia keratitis seen over two years, from September 1999 to September 2001. Corn...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:PURPOSE:The aim of this study was to isolate a novel mycobacteriophage and then explore its anti-tuberculosis (TB) potential. MATERIALS AND METHODS:Phage was isolated from enriched soil sample. A total of 36 mycobacterial strains obtained from clinical specimens were subjected to investigate the host range of phage by...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.180282
更新日期:2016-04-01 00:00:00
abstract::Therapeutic options for the treatment of melioidosis caused by Burkholderia pseudomallei are limited due to the inherent resistance conferred by this pathogen to various groups of antibiotics. Witnessing an increase in the number of microbiological culture-confirmed cases of melioidosis at our settings in the past few...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.188340
更新日期:2016-07-01 00:00:00
abstract::Probiotics are friendly live microorganisms (in most cases, bacteria) that are similar to beneficial micro-organisms found in the human gut, whenever consumed, have potential to confer benefit to the health of consumers by maintaining, or improving their intestinal microbial flora and are available to consumers mainly...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.4103/0255-0857.150868
更新日期:2015-02-01 00:00:00
abstract::The aim of this study was to explore baseline data, laboratory and molecular analyses to determine if any could serve as potential prognostic marker(s) for treatment response to second line tuberculosis regimens. Of a total number of 50 multi-drug resistant tuberculosis (MDR-TB) patients starting second-line drug MDR-...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.118899
更新日期:2013-10-01 00:00:00
abstract::In the present report, we have analysed C.trachomatis infection and HIV positivity among patients (n-143) who attended the STD clinic at the Institute of STDs, Government General Hospital, Chennai. HIV positivity rate was significantly high among those with chlamydial infection than in those without chlamydial infecti...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.13871
更新日期:2005-01-01 00:00:00
abstract:Introduction:Unavailability of optimal susceptibility testing (ST) challenges the clinical use of colistin. Broth microdilution (BMD), which is the reference for colistin ST, is inconvenient for diagnostics. Vitek2 and E-test although technically easier, are no longer recommended. Materials and Methods:For the evaluat...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_19_480
更新日期:2020-01-01 00:00:00
abstract:PURPOSE:The present study was undertaken to detect metallo-beta-lactamase (MBL) in nosocomial isolates of Pseudomonas aeruginosa by four different phenotypic methods. METHODS:Ninety-one consecutive P. aeruginosa isolates were subjected to susceptibility testing by disc-diffusion assay and Vitek 2. Imipenem resistance ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.39587
更新日期:2008-07-01 00:00:00
abstract::The nature of reporting of a microbiology laboratory depends upon the quality of the culture media used. Quality of media directly affects the observations and inferences drawn from the cultural characteristics of microorganisms. Checking of different parameters of media such as growth supporting characteristics, phys...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.4103/0255-0857.16586
更新日期:2005-07-01 00:00:00
abstract:PURPOSE:The objective of this study is to determine the role of quantitative cultures of non-bronchoscopic samples such as blinded bronchial sampling (BBS) and endotracheal aspirates (ETA) in the management of ventilator associated pneumonia (VAP). The study also evaluates the clinical diagnosis of VAP based on the inc...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.25226
更新日期:2006-04-01 00:00:00
abstract:PURPOSE:Increasing reports on New Delhi metallo-β-lactamase-1 (NDM-1) producing Escherichia coli constitute a serious threat to global health since it is found to be highly resistant to most of the currently available antibiotics including carbapenems. This study has been performed to find out the incidence blaNDM-1 in...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.115628
更新日期:2013-07-01 00:00:00
abstract::Rhinosporidiosis and its causative pathogen Rhinosporidium seeberi have been known for over a hundred years. Yet unresolved enigmas in rhinosporidiosis include the mode of infection, mechanisms of spread, mechanisms of immunity, some aspects of histopathology e.g. the significance of transepidermal elimination of spor...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract::Ocular infection with microsporidia has been documented in both immunocompetent and immunocompromised patients. Sources and mode of human infection with microsporidia have been difficult to ascertain although exposure to water may be an important risk factor. Of four genera that have been reported in human disease, on...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.90189
更新日期:2011-10-01 00:00:00
abstract:Background:The implementation of hospital infection prevention and control (IPC) in south Asia is not well described. We aimed to assess IPC programmes in hospitals in this region and explore opportunities for improvement. Methods:Attendees from hospitals in the South Asian Association for Regional Cooperation (SAARC)...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_18_82
更新日期:2018-10-01 00:00:00
abstract::Brevibacteria are a part of the normal skin flora and may be dismissed in blood cultures as contaminants. They have been reported as opportunistic pathogens in immunocompromised patients. We report a catheter-related bloodstream infection with Brevibacterium casei in a 6-year-old child with aplastic anaemia. Treatment...
journal_title:Indian journal of medical microbiology
pub_type:
doi:10.4103/ijmm.IJMM_20_292
更新日期:2020-04-01 00:00:00
abstract:PURPOSE:A statistical assessment of a disease is often necessary before resources can be allocated to any control programme. No literature on seasonal trends of gonorrhoea is available from India. OBJECTIVES:The objectives were (1) to determine, if any, seasonal trends were present in India (2) to describe factors con...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.154853
更新日期:2015-04-01 00:00:00
abstract:Background:Influenza viruses have emerged as virulent pathogens causing considerable burden across the world. A thorough understanding of the pattern in occurrence of influenza globally is the need of hour. The present study deals with analysis of the dynamics of Influenza virus, especially the influence of seasonal ch...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_19_226
更新日期:2019-07-01 00:00:00
abstract::Histoid leprosy is a variant of lepromatous leprosy, which develops as a result of resistance to dapsone monotherapy. Here we report two cases of lepromatous leprosy of histoid type, one with typical and another with atypical presentations. ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.31069
更新日期:2007-01-01 00:00:00
abstract::Porcine small intestinal sub-mucosa is a cell-free collagen matrix that has demonstrated its ability as a scaffold material. Transplantation poses special hazards because grafted tissues and organs transmit pathogens efficiently, especially viruses. Rotavirus is thought to be confined to the intestine, causing acute d...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.49430
更新日期:2009-04-01 00:00:00
abstract:Purpose:The Revised National Tuberculosis Control Programme (RNTCP) is now introducing daily fixed-dose regimen instead of Directly Observed Treatment, Short Course (DOTS) regimen for treatment of drug-sensitive tuberculosis (TB) in India. It would be beneficial to understand the drawbacks, barriers and advantages of t...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_18_65
更新日期:2018-04-01 00:00:00