Detection of porcine rotavirus from tissue and faecal specimens.

Abstract:

:Porcine small intestinal sub-mucosa is a cell-free collagen matrix that has demonstrated its ability as a scaffold material. Transplantation poses special hazards because grafted tissues and organs transmit pathogens efficiently, especially viruses. Rotavirus is thought to be confined to the intestine, causing acute diarrhoea. The purpose of this study was to evaluate the porcine intestinal tissue scaffold for Rotavirus and to study the incidence of this virus among pig herds. Only one isolate was successfully adapted to grow in cell line MA 104 from faecal samples. This isolate was further confirmed by reverse transcriptase polymerase chain reaction and sequence analysis.

journal_name

Indian J Med Microbiol

authors

Prabha S,Verghese S

doi

10.4103/0255-0857.49430

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

149-52

issue

2

eissn

0255-0857

issn

1998-3646

pii

IndianJMedMicrobiol_2009_27_2_149_49430

journal_volume

27

pub_type

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