Abstract:
BACKGROUND:Culture filtrate proteins (CFPs) of Mycobacterium tuberculosis are potential vaccine candidates. OBJECTIVE:The aim was to study the influence of iron levels on CFPs and assess the immuno-protective potential of defined antigenic fractions from high (8 μg Fe/mL) and low iron (0.02 μg Fe / mL) cultures of M. tuberculosis. MATERIALS AND METHODS:The CFPs of M. tuberculosis from high (CFP-high) and low (CFP-low) iron conditions were first compared to identify iron-regulated proteins and then fractionated to obtain ten antigen pools (CF-Ags H1- H5 and L1-L5) that were used to assess the immune response of TB patients and normal healthy controls. RESULTS:Iron limitation resulted in the up-regulation of two novel iron-regulated low-molecular-weight proteins Irp-1 (in CF-Ag L4) and Irp-2 (in CF-Ag L5) and repression of two ESAT proteins (identified with monoclonal antibody HYB 76.8). The median stimulation indices (SIs) against most of the CF-Ags were high in pulmonary TB patients. The CF-Ags L1 and L2 showed statistically significant SI (P values of 0.0027 and 0.0029 respectively); the % case recognition was high with these antigens as well as with L4 ( P = 0.0275). IFN-γ in response to these CF-Ags was significantly high in the endemic normals; maximal expression was seen with CF-Ag L5 (median value of 233 pg mL -1 ) that was higher than the corresponding H5 (140 pg mL -1 ) and H3 and L3 (205 and 206 pg mL -1 respectively). CONCLUSIONS:CF-Ags L5, H3 and L3 showed immuno-protective potential in this geographical location.
journal_name
Indian J Med Microbioljournal_title
Indian journal of medical microbiologyauthors
Duggirala S,Venu K,Subhakar K,Sritharan Mdoi
10.4103/0255-0857.99495subject
Has Abstractpub_date
2012-07-01 00:00:00pages
323-31issue
3eissn
0255-0857issn
1998-3646pii
IndianJMedMicrobiol_2012_30_3_323_99495journal_volume
30pub_type
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abstract::[This corrects the article DOI: 10.4103/0255-0857.188314.]. ...
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