Abstract:
:Detecting high-risk-human papillomavirus (HPV) types has become an integral part of the cervical cancer screening programmes. This study aimed to develop a multiplex polymerase chain reaction (PCR) for identification of HPV types 16 and 18 along with the beta globin gene in formalin-fixed and paraffin-embedded cervical biopsy specimens. A total of 59 samples from patients with cervical abnormalities were tested. HPV 16 positivity was 50% in cervical cancers and 52.9% in cervical intraepithelial neoplasia. Our multiplex PCR protocol can be used as a simple and cost-effective tool for high-risk-HPV detection in cervical cancer screening programmes.
journal_name
Indian J Med Microbioljournal_title
Indian journal of medical microbiologyauthors
Singh MP,Gupta N,Deepak T,Kumar A,Ratho RKdoi
10.4103/ijmm.IJMM_15_277subject
Has Abstractpub_date
2017-01-01 00:00:00pages
113-115issue
1eissn
0255-0857issn
1998-3646pii
IndianJMedMicrobiol_2017_35_1_113_202322journal_volume
35pub_type
杂志文章abstract::A 23-year-old male patient who was a follow-up case of neurosurgery presented to our emergency department with a history of high-grade fever and clinical features of meningitis for 1 week. The cerebrospinal fluid (CSF) was sent to our laboratory for culture. The culture demonstrated growth of 1-2 mm in diameter light ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.115653
更新日期:2013-07-01 00:00:00
abstract:PURPOSE:The present study was undertaken to determine the prevalence of asymptomatic bacteriuria in school going children of different age groups and sex and to isolate the organisms responsible for asymptomatic bacteriuria and to know their antimicrobial susceptibility pattern. METHODS:A total of 1817 school children...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract:PURPOSE:To determine the risk factors, microbiological features, clinical features and other epidemiological characteristics of Nocardia keratitis seen at a tertiary eye care centre in south India. METHODS:We evaluated 31 patients with Nocardia keratitis seen over two years, from September 1999 to September 2001. Corn...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Acute pancreatitis occasionally presents as pancreatic abscess with complications like pleural effusion and ascites. There are several pre-disposing factors, the most common being cholelithiaisis, alcohol abuse, infective causes, trauma, and metabolic causes such as diabetic ketoacidosis, while some cases are idiopath...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.58735
更新日期:2010-01-01 00:00:00
abstract::Serum samples from 465 subjects aged between 1 and 25 years were tested for antibody against hepatitis A virus (HAV) [anti-HAV IgG and IgM] to determine the seroprevalence of HAV antibody and do a cost-benefit analysis for decision making about vaccination against HAV among the general population of Bangladesh. A high...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract:PURPOSE:Biochemical or nucleic acid based diagnostic techniques for MAC infection are unsatisfactory. This study aims to identify and evaluate M. avium secretory protein(s) of diagnostic potential, so as to develop a rapid and simple method for diagnosis of MAC infection. MATERIAL AND METHODS:Initially, a specific pro...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.66478
更新日期:2010-07-01 00:00:00
abstract:PURPOSE:The present study was undertaken to detect metallo-beta-lactamase (MBL) in nosocomial isolates of Pseudomonas aeruginosa by four different phenotypic methods. METHODS:Ninety-one consecutive P. aeruginosa isolates were subjected to susceptibility testing by disc-diffusion assay and Vitek 2. Imipenem resistance ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.39587
更新日期:2008-07-01 00:00:00
abstract:PURPOSE:Escherichia coli (E. coli) O157:H7 is gram-negative enteric pathogen producing different types of Shiga toxin. This bacterium is the most corporate cause of haemorrhagic colitis in human. Administration of antibiotics (particularly sulfa drugs) against this pathogen is a debatable topic as this may increase the...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.150895
更新日期:2015-02-01 00:00:00
abstract:PURPOSE:Vancomycin-resistant enterococci (VRE) pose an emerging problem in hospitals worldwide. The present study was undertaken to determine the occurrence, species prevalence, antibacterial resistance, and phenotypic and genetic characteristics of VRE isolated in Riyadh hospitals, KSA. MATERIALS AND METHODS:Two hund...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.93032
更新日期:2012-01-01 00:00:00
abstract::Severe acute respiratory syndrome (SARS) is a form of atypical pneumonia that apparently originated in Guangdong Province of the People's Republic of China in late 2002. This first came to the world's attention in late February 2003, and has since spread worldwide. As of June 23rd 2003, the disease had been reported f...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00
abstract:PURPOSE:Humans are the only known natural hosts of hepatitis C virus (HCV). This study was undertaken to examine the frequencies of human leucocyte antigens (HLAs) Class I and Class II genotype profiles in anti-HCV-infected patients of Northern India. MATERIALS AND METHODS:From a period of January 2013 to August 2014,...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.188317
更新日期:2016-07-01 00:00:00
abstract::Emergence of extended spectrum beta lactamases (ESBLs) producing strains of gram negative bacteria, as one of the leading cause of septicaemia often complicates the clinical and therapeutic outcome. The present study was undertaken to investigate the prevalence of ESBLs in bacteria isolated from neonatal septicaemic c...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.43578
更新日期:2008-10-01 00:00:00
abstract:PURPOSE:The objective of this study is to determine the role of quantitative cultures of non-bronchoscopic samples such as blinded bronchial sampling (BBS) and endotracheal aspirates (ETA) in the management of ventilator associated pneumonia (VAP). The study also evaluates the clinical diagnosis of VAP based on the inc...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.25226
更新日期:2006-04-01 00:00:00
abstract::Aspergilloma is a fungal ball that usually forms in a preformed stationary cavity, mostly in lung and paranasal sinuses. We report a rare case of primary orbital Aspergilloma following exenteration for an invasive ocular surface squamous neoplasia, clinically mimicking a recurrence of the tumor. The fungal ball showed...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::Ocular infection with microsporidia has been documented in both immunocompetent and immunocompromised patients. Sources and mode of human infection with microsporidia have been difficult to ascertain although exposure to water may be an important risk factor. Of four genera that have been reported in human disease, on...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.90189
更新日期:2011-10-01 00:00:00
abstract::Hemophagocytic lymphohistiocytosis (HLH) triggered by Parvovirus B19 and Epstein-Barr virus co-infection is rare and unknown in infants. A 2-month-old male infant with fever, rash, bicytopenia and hepato-splenomegaly died owing to diagnostic dilemmas. Hence simply testing for hyperferritinaemia and hypertriglyceridemi...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.129819
更新日期:2014-04-01 00:00:00
abstract:PURPOSE:Enteric parasitic infestation is a major public health problem in developing countries. Parasites such as Cryptosporidium spp., Cyclospora spp., Cystoisospora spp. and Microsporidia may cause severe diarrhoea among immunocompromised patients. There is scanty data on their frequency among immunocompetent patient...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.174114
更新日期:2016-01-01 00:00:00
abstract:AIM:To evaluate E-test as a tool for rapid determination of drug susceptibility against the conventional LJ method focusing on reliability, expense, ease of standardization and performance of the technique in low resource settings. MATERIALS AND METHODS:A total of 74 clinical isolates (2004-2005) of Mycobacterium tube...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.58725
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVE:The purpose of our study was to compare various laboratory diagnostic methods, namely histopathological examination, Ziehl-Neelsen (ZN) stain, AFB culture by conventional Lowenstein-Jensen (LJ) method and fluorescence-based mycobacterial growth indicator tube (MGIT) technique and polymerase chain reaction (PC...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.90173
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND:Surveillance of antimicrobial resistance (AMR) is of great importance. Pseudomonas aeruginosa and Acinetobacter baumannii are important pathogens and emergence of resistance in these have increased the morbidity and mortality rates. This surveillance study was initiated by the Government of India - Indian Co...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,多中心研究
doi:10.4103/0255-0857.195376
更新日期:2016-10-01 00:00:00
abstract:INTRODUCTION:Recent years have seen a rise of coagulase-negative staphylococci (CoNS) from common contaminants to agents of nosocomial blood stream infections (BSI's). Molecular typing and establishing a correlation with antibiotic resistance is essential particularly in countries like India where genotyping studies fo...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.195374
更新日期:2016-10-01 00:00:00
abstract::Tubercular splenic abscess in an immunocompetent person, especially when the patient is without any symptoms, is a rare clinical entity. We report a case of isolated tubercular splenic abscess in a 38-year-old asymptomatic healthy female and emphasize the importance of careful smear examination. The patient had come t...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.62501
更新日期:2010-04-01 00:00:00
abstract::We report here the emergence of a new focus of cutaneous leishmaniasis (CL) due to Leishmania tropica (L. tropica) in the Ajmer city of Rajasthan, India, a previously non-endemic area. Between January-February 2006, 13 new indigenously acquired cases of CL were diagnosed among the patients attending the Skin and STD d...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.34774
更新日期:2007-07-01 00:00:00
abstract::Varied clinical presentations of Penicillium marneffei, an opportunistic pathogen in HIV disease has been rarely described in literature. We report a patient with advanced AIDS who presented to us with prolonged fever and had features of an acute abdomen. On radiologic imaging he had features of intestinal obstruction...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.40538
更新日期:2008-04-01 00:00:00
abstract::Disseminated cases of histoplasmosis in acquired immune deficiency syndrome (AIDS) are rarely reported from India. Most of these cases report isolation of this fungus from the bone marrow, lymph node aspirate, spleenic aspirate, and biopsies. We report isolation of Histoplasma capsulatum from the blood of an AIDS pati...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.81792
更新日期:2011-04-01 00:00:00
abstract::A male infant admitted with pyogenic meningitis with protein energy malnutrition developed fatal infection due to Salmonella enteritidis. The same organism was isolated from CSF and blood cultures. ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2001-07-01 00:00:00
abstract:BACKGROUND:Detecting plasmid-mediated AmpC (pAmpC) β-lactamase-producing organism is important for optimal infection control and providing accurate and effective treatment option for physicians. OBJECTIVES:The aim of this study was to investigate the prevalence of pAmpC β-lactamase and compare the results of boronic a...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.108723
更新日期:2013-01-01 00:00:00
abstract::Antibiotic resistance is a major problem in clinical health settings. Interestingly the origin of many of antibiotic resistance mechanisms can be traced back to non-pathogenic environmental organisms. Important factors leading to the emergence and spread of antibiotic resistance include absence of regulation in the us...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.4103/0255-0857.103756
更新日期:2012-10-01 00:00:00
abstract:BACKGROUND:A disease outbreak of A (H1N1) PDM09 was reported in Andaman and Nicobar islands in 2009 with an attack rate of 33.5% among settler population and 26.3% among the aboriginal Nicobarese tribe. During the ongoing outbreak of A (H1N1) PDM09 disease in different parts of the world, a subject working in Dubai cit...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.148417
更新日期:2015-01-01 00:00:00
abstract::Humanity has been at the receiving end of many viral diseases since ages. Sudden emergence and re-emergence of new viral diseases in human beings has surprised the medical scientists from time to time. "Avian influenza" or "Bird flu" by H5N1 epidemics is one such surprise. Although many aspects about this disease are ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2004-07-01 00:00:00