Multiplex polymerase chain reaction for the detection of high-risk-human papillomavirus types in formalin-fixed paraffin-embedded cervical tissues.

Abstract:

:Detecting high-risk-human papillomavirus (HPV) types has become an integral part of the cervical cancer screening programmes. This study aimed to develop a multiplex polymerase chain reaction (PCR) for identification of HPV types 16 and 18 along with the beta globin gene in formalin-fixed and paraffin-embedded cervical biopsy specimens. A total of 59 samples from patients with cervical abnormalities were tested. HPV 16 positivity was 50% in cervical cancers and 52.9% in cervical intraepithelial neoplasia. Our multiplex PCR protocol can be used as a simple and cost-effective tool for high-risk-HPV detection in cervical cancer screening programmes.

journal_name

Indian J Med Microbiol

authors

Singh MP,Gupta N,Deepak T,Kumar A,Ratho RK

doi

10.4103/ijmm.IJMM_15_277

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

113-115

issue

1

eissn

0255-0857

issn

1998-3646

pii

IndianJMedMicrobiol_2017_35_1_113_202322

journal_volume

35

pub_type

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