Abstract:
:Legionella pneumophila is one of the important pathogen responsible for community -acquired pneumonia attributing for 1-5% of cases. Since early and accurate therapy reduces mortality, rapid and reliable diagnostic methods are needed. A total of 134 samples of blood, urine and respiratory tract fluids were collected. Blood was tested for IgG, IgM and IgA antibodies using commercially available kits. A total of 8 (6%) samples were found to be positive for L. pneumophila by quantitative reverse transcription polymerase chain reaction (qRT-PCR), compared to conventional PCR where 6 (4.4%) samples were positive. Serology was positive in a total of 32 (23%) cases though only 3 (2.2%) of the PCR-positive cases were positive by serology as well. These results suggest that real-time PCR can detect Legionella infection early in the course of the disease before serological response develops.
journal_name
Indian J Med Microbioljournal_title
Indian journal of medical microbiologyauthors
Angrup A,Chaudhry R,Sharma S,Valavane A,Passi K,Padmaja K,Javed S,Dey AB,Dhawan B,Kabra SKdoi
10.4103/0255-0857.195353subject
Has Abstractpub_date
2016-10-01 00:00:00pages
539-543issue
4eissn
0255-0857issn
1998-3646pii
IndianJMedMicrobiol_2016_34_4_539_195353journal_volume
34pub_type
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