Abstract:
OBJECTIVE:To design a resistance gene detection chip that could, in parallel, detect common clinical drug resistance genes of Gram-negative bacteria. MATERIALS AND METHODS:Seventy clinically significant Gram-negative bacilli (Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Pseudomonas aeruginosa, Acinetobacter baumannii) were collected. According to the known resistance gene sequences, we designed and synthesized primers and probes, which were used to prepare resistance gene detection chips, and finally we hybridized and scanned the gene detection chips. RESULTS:The results between the gene chip and polymerase chain reaction (PCR) were compared. The rate was consistently 100% in the eight kinds of resistance genes tested (TEM, SHV, CTX-M, DHA, CIT, VIM, KPC, OXA-23). One strain of Pseudomonas aeruginosa had the IMP, but it was not found by gene chip. CONCLUSION:The design of Gram-negative bacteria-resistant gene detection chip had better application value.
journal_name
Indian J Med Microbioljournal_title
Indian journal of medical microbiologyauthors
Ting C,Jun A,Shun Zdoi
10.4103/0255-0857.115230subject
Has Abstractpub_date
2013-04-01 00:00:00pages
142-7issue
2eissn
0255-0857issn
1998-3646pii
IndianJMedMicrobiol_2013_31_2_142_115230journal_volume
31pub_type
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journal_title:Indian journal of medical microbiology
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journal_title:Indian journal of medical microbiology
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journal_title:Indian journal of medical microbiology
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doi:10.4103/0255-0857.37344
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journal_title:Indian journal of medical microbiology
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journal_title:Indian journal of medical microbiology
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pub_type: 杂志文章
doi:10.4103/0255-0857.150895
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.148374
更新日期:2015-01-01 00:00:00
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,评审
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_16_282
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journal_title:Indian journal of medical microbiology
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doi:10.4103/ijmm.IJMM_17_99
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
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doi:10.4103/ijmm.IJMM_17_412
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.118899
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.4103/ijmm.IJMM_19_373
更新日期:2019-04-01 00:00:00
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_16_96
更新日期:2017-10-01 00:00:00
abstract::Great advances in technology produce unique challenges. Every technology also has a dual use, which needs to be understood and managed to extract maximum benefits for mankind and the development of civilization. The achievements of physicists in the mid-20th century resulted in the nuclear technology, which gave us th...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.4103/0255-0857.90155
更新日期:2011-10-01 00:00:00
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.58725
更新日期:2010-01-01 00:00:00
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.29390
更新日期:2006-10-01 00:00:00
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.39587
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,多中心研究
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更新日期:2016-10-01 00:00:00
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.180353
更新日期:2016-04-01 00:00:00
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_19_226
更新日期:2019-07-01 00:00:00