Comparison of nested polymerase chain reaction and real-time polymerase chain reaction targeting 47kda gene for the diagnosis of scrub typhus.

Abstract:

Introduction:Scrub typhus is a zoonotic infection caused by Orientia tsutsugamushi which is transmitted by Leptotrombidium mites. The disease manifests as a mild-to-severe illness with non-specific clinical symptoms. Rapid diagnosis and prompt treatment are essential for patient management. Both serological and molecular methods are used for the diagnosis of scrub typhus. The present study assessed the usefulness of detection of the gene encoding the 47kDa outer-membrane protein (OMP) for the laboratory diagnosis of scrub typhus. Materials and Methods:Nested polymerase chain reaction (nPCR) and real-time PCR targeting 47 kDa OMP antigen gene of O. tsutsugamushi were performed on ethylenediaminetetraacetic acid blood samples. Results:Six of the 103 (5.8%) patients showed the presence of 47kDa gene by nPCR. Seventy of 103 (67.9%) cases showed the presence of 47kDa gene by qPCR. Among the 70 positive cases, the majority of them were females (40/70, 57.1%). The highest number of positive cases was observed during October-February. Conclusion:Real-time PCR targeting O. tsutsugamushi-specific 47-kDa gene is more sensitive than nPCR and may be the assay of choice for the detection of the organism in patients with suspected scrub typhus.

journal_name

Indian J Med Microbiol

authors

Srinivasan S,Kalaimani S,Jude Prakash JA,Menon T

doi

10.4103/ijmm.IJMM_19_170

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

50-53

issue

1

eissn

0255-0857

issn

1998-3646

pii

IndianJMedMicrobiol_2019_37_1_50_264488

journal_volume

37

pub_type

杂志文章
  • Corynebacterium striatum: an emerging nosocomial pathogen in a case of laryngeal carcinoma.

    abstract::Corynebacterium striatum is an emerging nosocomial pathogen associated with wound infections, pneumonia and meningitis. It is also a multidrug-resistant pathogen causing high morbidity. This is a report of an unusual case of wound infection in a patient with laryngeal carcinoma. Accurate diagnosis of the infection and...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.136589

    authors: Biswal I,Mohapatra S,Deb M,Dawar R,Gaind R

    更新日期:2014-07-01 00:00:00

  • Comparison of various microbiological tests including polymerase chain reaction for the diagnosis of osteoarticular tuberculosis.

    abstract:PURPOSE:To evaluate the utility of the polymerase chain reaction (PCR) test for diagnosing osteoarticular tuberculosis (TB). METHODS:Clinical samples (synovial tissue and synovial fluid) obtained from 23 cases of suspected osteoarticular tuberculosis were subjected to Ziehl Neelsen (ZN) smear examination, radiometric ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Negi SS,Gupta S,Khare S,Lal S

    更新日期:2005-10-01 00:00:00

  • KatG protein: a novel marker for differential diagnosis of Mycobacterium avium complex infection.

    abstract:PURPOSE:Biochemical or nucleic acid based diagnostic techniques for MAC infection are unsatisfactory. This study aims to identify and evaluate M. avium secretory protein(s) of diagnostic potential, so as to develop a rapid and simple method for diagnosis of MAC infection. MATERIAL AND METHODS:Initially, a specific pro...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.66478

    authors: Gupta K,Verma I,Khuller G,Mahajan R

    更新日期:2010-07-01 00:00:00

  • Identification of Raoultella spp.: Comparison of three methods.

    abstract:Background:Raoultella is a Gram-negative bacteria, which commonly occur in the natural environment such as water, soil and on plants. In recent years, Raoultella spp. gained more interest. There is also an increasing number of publications describing mainly clinical cases involving these bacteria. Identification of Rao...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_17_99

    authors: Sekowska A,Mikucka A,Gospodarek-Komkowska E

    更新日期:2018-04-01 00:00:00

  • Trends in antimicrobial susceptibility of Salmonella Typhi from North India (2001-2012).

    abstract:PURPOSE:Enteric fever is endemic in India with Salmonella Typhi being the major causative agent. Antibiotic therapy constitutes the mainstay of management. The present study was undertaken to find the susceptibility profile of Salmonella enterica var Typhi (S. Typhi) blood isolates in a tertiary care hospital between J...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.129799

    authors: Singhal L,Gupta PK,Kale P,Gautam V,Ray P

    更新日期:2014-04-01 00:00:00

  • Hepatitis C virus and its genotypes in chronic liver disease patients from Meghalaya, Northeast India.

    abstract:Background and Objectives:Hepatitis C virus (HCV) is an important cause of chronic liver disease (CLD). Although Northeast India is believed to be a HCV hotspot, the proportion of HCV infection and the distribution of HCV genotypes in CLD cases from the region are not known. The objectives of the study were to determin...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_17_371

    authors: Barman B,Bora K,Lynrah KG,Lyngdoh WV,Jamil M

    更新日期:2018-07-01 00:00:00

  • Regulatory categories of probiotics across the globe: a review representing existing and recommended categorization.

    abstract::Probiotics are friendly live microorganisms (in most cases, bacteria) that are similar to beneficial micro-organisms found in the human gut, whenever consumed, have potential to confer benefit to the health of consumers by maintaining, or improving their intestinal microbial flora and are available to consumers mainly...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.4103/0255-0857.150868

    authors: Arora M,Baldi A

    更新日期:2015-02-01 00:00:00

  • National survey of infection control programmes in South Asian association for Regional Cooperation countries in the era of patient safety.

    abstract:Background:The implementation of hospital infection prevention and control (IPC) in south Asia is not well described. We aimed to assess IPC programmes in hospitals in this region and explore opportunities for improvement. Methods:Attendees from hospitals in the South Asian Association for Regional Cooperation (SAARC)...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_18_82

    authors: Gupta SK,Siddharth V,Belagere MR,Stewardson AJ,Kant S,Singh S,Singh N

    更新日期:2018-10-01 00:00:00

  • Gut microbiota and its mysteries.

    abstract::Gut microbiota are microorganisms that inhabit the gut; they coexist peacefully with the host, thereby contributing to the health and well-being of individuals. Bacteroidetes and Firmicutes largely dominate the gut microbial flora. The intestinal flora promotes intestinal mucosal integrity, provides essential nutrient...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.4103/ijmm.IJMM_19_373

    authors: Pushpanathan P,Mathew GS,Selvarajan S,Seshadri KG,Srikanth P

    更新日期:2019-04-01 00:00:00

  • An ocular cysticercosis case: Caused by Asian genotype of Taenia solium.

    abstract::An ocular cysticercosis case of a 42-year-old male, who presented with anterior uveitis is being reported. Microscopical examination of the cyst revealed presence of only one hooklet suggestive of T. solium cysticercus. Mitochondrial DNA analysis confirmed it to be T. solium cysticercus of Asian genotype. This is the ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.167319

    authors: Sharma M,Beke N,Khurana S,Bhatti HS,Sehgal R,Malla N

    更新日期:2015-10-01 00:00:00

  • The seroepidemiology of Rubella in Amritsar (Punjab).

    abstract::Seroprevalence of Rubella was determined in 580 women including 80 women of medical community of district Amritsar, by ELISA test. The overall Rubella IgG seropositivity was 68.8% while in women of medical community it was 80%. Maximum number of women were seropositive (77.2%) in age group 26-35 years. Significantly h...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Singla N,Jindal N,Aggarwal A

    更新日期:2004-01-01 00:00:00

  • Neonatal listeriosis followed by nosocomial infection.

    abstract::Neonatal listeriosis is widely reported, but this is the first case reported in Serbia. A newborn developed respiratory distress syndrome 2 hours after delivery and was admitted to the neonatal intensive care unit. Initial empirical therapy was inappropriate. Consequently, on the second day, the patient developed meni...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.115229

    authors: Dinic M,Stankovic S

    更新日期:2013-04-01 00:00:00

  • Molecular characterisation of antimicrobial resistance in Pseudomonas aeruginosa and Acinetobacter baumannii during 2014 and 2015 collected across India.

    abstract:BACKGROUND:Surveillance of antimicrobial resistance (AMR) is of great importance. Pseudomonas aeruginosa and Acinetobacter baumannii are important pathogens and emergence of resistance in these have increased the morbidity and mortality rates. This surveillance study was initiated by the Government of India - Indian Co...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.4103/0255-0857.195376

    authors: Pragasam AK,Vijayakumar S,Bakthavatchalam YD,Kapil A,Das BK,Ray P,Gautam V,Sistla S,Parija SC,Walia K,Ohri VC,Anandan S,Veeraraghavan B

    更新日期:2016-10-01 00:00:00

  • Detection of plasmid-mediated AmpC β-lactamase in Escherichia coli and Klebsiella pneumoniae.

    abstract:BACKGROUND:Detecting plasmid-mediated AmpC (pAmpC) β-lactamase-producing organism is important for optimal infection control and providing accurate and effective treatment option for physicians. OBJECTIVES:The aim of this study was to investigate the prevalence of pAmpC β-lactamase and compare the results of boronic a...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.108723

    authors: Yilmaz NO,Agus N,Bozcal E,Oner O,Uzel A

    更新日期:2013-01-01 00:00:00

  • Characterisation of antimicrobial resistance in Salmonellae during 2014-2015 from four centres across India: An ICMR antimicrobial resistance surveillance network report.

    abstract:PURPOSE:The main purpose of this study was to establish 'Antimicrobial Resistance Surveillance Network' in India and to monitor the antimicrobial susceptibility profile of clinical isolates to establish a national network across the country for monitoring antimicrobial resistance in Salmonella. MATERIALS AND METHODS:T...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.4103/ijmm.IJMM_16_382

    authors: Dahiya S,Sharma P,Kumari B,Pandey S,Malik R,Manral N,Veeraraghavan B,Pragasam AK,Ray P,Gautam V,Sistla S,Parija SC,Walia K,Ohri V,Das BK,Sood S,Kapil A

    更新日期:2017-01-01 00:00:00

  • Human leucocyte antigen Class I and II alleles associated with anti-hepatitis C virus-positive patients of North India.

    abstract:PURPOSE:Humans are the only known natural hosts of hepatitis C virus (HCV). This study was undertaken to examine the frequencies of human leucocyte antigens (HLAs) Class I and Class II genotype profiles in anti-HCV-infected patients of Northern India. MATERIALS AND METHODS:From a period of January 2013 to August 2014,...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.188317

    authors: Chowdhry M,Makroo RN,Singh M,Agrawal S,Kumar M,Thakur Y

    更新日期:2016-07-01 00:00:00

  • Geographically distinct North-East Indian Helicobacter pylori strains are highly sensitive to clarithromycin but are levofloxacin resistant.

    abstract:Purpose:Helicobacter pylori causes various gastro-intestinal diseases. Antibiotic resistance to commonly used antibiotics for the treatment of H. pylori infection is the major cause for treatment failure. The aim of this study is to determine the antimicrobial susceptibility pattern for clarithromycin and levofloxacin ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_19_158

    authors: Mahant S,Sharma AK,Gehlot V,Mukhopadhyay AK,Chhawchharia A,Dutta S,Agarwal A,Som A,Das K,Das R

    更新日期:2019-07-01 00:00:00

  • Extended spectrum -lactamases (ESBL) - an emerging threat to clinical therapeutics.

    abstract::Extended spectrum -lactamases (ESBLs) are plasmid mediated, TEM and SHV derived enzymes, first isolated in Western Europe in mid 1980s, most commonly in Klebsiella spp., followed by Escherichia coli. These enzymes are capable of hydrolyzing broad spectrum cephalosporins and monobactams but inactive against cephamycins...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Chaudhary U,Aggarwal R

    更新日期:2004-04-01 00:00:00

  • Superantigens - concepts, clinical disease and therapy.

    abstract::The term superantigen was introduced in microbiology and immunology only a decade ago. The unique feature of superantigen is that it bypasses the antigen processing mechanism and specifically binds to TCR v segment and forms a trimolecular complex along with major histocompatibility complex class II. Since its discove...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Hemalatha V,Srikanth P,Mallika M

    更新日期:2004-10-01 00:00:00

  • A study of tubercular lymphadenitis: a comparison of various laboratory diagnostic modalities with a special reference to tubercular polymerase chain reaction.

    abstract:OBJECTIVE:The purpose of our study was to compare various laboratory diagnostic methods, namely histopathological examination, Ziehl-Neelsen (ZN) stain, AFB culture by conventional Lowenstein-Jensen (LJ) method and fluorescence-based mycobacterial growth indicator tube (MGIT) technique and polymerase chain reaction (PC...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.90173

    authors: Patwardhan SA,Bhargava P,Bhide VM,Kelkar DS

    更新日期:2011-10-01 00:00:00

  • Drug resistance profile of human Mycobacterium avium complex strains from India.

    abstract:PURPOSE:To determine minimum inhibitory concentration (MIC) of various anti-tuberculosis drugs for Mycobacterium avium complex (MAC) strains isolated from clinical samples. METHODS:Forty-nine human isolates of MAC were tested for susceptibility to nine chemotherapeutic agents. All isolates were from Indian patients su...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.32716

    authors: Venugopal D,Kumar S,Isa M,Bose M

    更新日期:2007-04-01 00:00:00

  • Molecular characterisation and clinical correlates of rotavirus in children and adults in a tertiary care centre, Chennai, South India.

    abstract:AIMS:This study was undertaken to determine the rate of detection of rotavirus causing diarrhoea among children and adults, identify the common genotypes circulating and determine clinical correlates. SETTINGS AND DESIGN:This is a cross-sectional study in a tertiary care centre. MATERIALS AND METHODS:Stool samples we...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_16_51

    authors: Selvarajan S,Reju S,Pushpanathan P,Arumugam R,Padmanabhan R,Kothandaramanujam SM,Srikanth P,Kang G

    更新日期:2017-04-01 00:00:00

  • Emergence of influenza A (H1N1) PDM09 in the remote Islands of India--a molecular approach.

    abstract:BACKGROUND:A disease outbreak of A (H1N1) PDM09 was reported in Andaman and Nicobar islands in 2009 with an attack rate of 33.5% among settler population and 26.3% among the aboriginal Nicobarese tribe. During the ongoing outbreak of A (H1N1) PDM09 disease in different parts of the world, a subject working in Dubai cit...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.148417

    authors: Muruganandam N,Bhattacharya D,Chaaithanya IK,Bhattacharya H,Reesu R,Maile A,Bharathi GS,Sugunan AP,Vijayachari P

    更新日期:2015-01-01 00:00:00

  • Injection site abscess due to Mycobacterium fortuitum: a case report.

    abstract::Injection abscess is an iatrogenic infection occurring as an isolated case or as cluster outbreak. These infections occur due to contaminated injectables or lapse in sterilisation protocol. While pathogens such as Pseudomonas, Klebsiella, E. coli, and S. aureus are the usual causative agents, unusual organisms such as...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Devi DR,Indumathi VA,Indira S,Babu PR,Sridharan D,Belwadi MR

    更新日期:2003-04-01 00:00:00

  • Severe acute respiratory syndrome.

    abstract::Severe acute respiratory syndrome (SARS) is a form of atypical pneumonia that apparently originated in Guangdong Province of the People's Republic of China in late 2002. This first came to the world's attention in late February 2003, and has since spread worldwide. As of June 23rd 2003, the disease had been reported f...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Thomas PA

    更新日期:2003-07-01 00:00:00

  • Treatment of discarded blood units: disinfection with hypochlorite / formalin versus steam sterilization.

    abstract::Blood bank regulations and bio medical waste rules of India advocate disinfection of contaminated blood units. Incineration is not recommended due to poly-vinyl chloride (PVC) content of blood bags. This study was designed to evaluate the efficacy of chemical disinfection of blood units deliberately contaminated with ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Chitnis V,Chitnis S,Patil S,Chitnis D

    更新日期:2003-10-01 00:00:00

  • The non-association of Panton-Valentine leukocidin and mecA genes in the genome of Staphylococcus aureus from hospitals in South Western Nigeria.

    abstract:PURPOSE:Virulence genes play important roles in pathogenesis of infections caused by S. aureus. The aim of this study was to determine the prevalence of PVL, eta and mecA genes in S. aureus isolated from patients in South-Western Nigeria. MATERIALS AND METHODS:In this study, a total of 116 S. aureus isolates from the ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.96675

    authors: Terry Alli OA,Ogbolu DO,Mustapha JO,Akinbami R,Ajayi AO

    更新日期:2012-04-01 00:00:00

  • High prevalence of hepatitis A virus antibody among Bangladeshi children and young adults warrants pre-immunization screening of antibody in HAV vaccination strategy.

    abstract::Serum samples from 465 subjects aged between 1 and 25 years were tested for antibody against hepatitis A virus (HAV) [anti-HAV IgG and IgM] to determine the seroprevalence of HAV antibody and do a cost-benefit analysis for decision making about vaccination against HAV among the general population of Bangladesh. A high...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Ahmed M,Munshi SU,Nessa A,Ullah MS,Tabassum S,Islam MN

    更新日期:2009-01-01 00:00:00

  • Combination antifungals as an effective means of salvage in paediatric leukaemia patients with invasive fungal infections.

    abstract::Invasive fungal infections (IFIs) are an important cause of morbidity and mortality in paediatric leukaemias. Antifungal combinations to treat these patients are being explored. Fourteen children with leukaemias and IFIs were treated with a combination of antifungal agents at our centre. The first antifungal was ampho...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_18_157

    authors: Meena JP,Gupta AK,Jana M,Seth R

    更新日期:2019-01-01 00:00:00

  • Chikungunya fever: a re-emerging viral infection.

    abstract::Chikungunya (CHIK) fever is a re-emerging viral disease characterized by abrupt onset of fever with severe arthralgia followed by constitutional symptoms and rash lasting for 1-7 days. The disease is almost self-limiting and rarely fatal. Chikungunya virus (CHIKV) is a RNA virus belonging to family Togaviridae, genus ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.4103/0255-0857.38850

    authors: Chhabra M,Mittal V,Bhattacharya D,Rana U,Lal S

    更新日期:2008-01-01 00:00:00