Abstract:
PURPOSE:Biochemical or nucleic acid based diagnostic techniques for MAC infection are unsatisfactory. This study aims to identify and evaluate M. avium secretory protein(s) of diagnostic potential, so as to develop a rapid and simple method for diagnosis of MAC infection. MATERIAL AND METHODS:Initially, a specific protein band of approximately 80-85 kDa was recognised by differential immunoblotting; which was subjected to anion exchange column chromatography for purification of proteins. After fractionisation using SDS-PAGE and electroelution, blast search was carried out. Further immunoreactivity studies were done with M. avium and Mtb infected mice sera. Clinical utilisation of separated protein was evaluated by conducting indirect ELISA with serum samples from mycobacterial infected patients. RESULTS:A specific 81.6 kDa protein, shown to be catalase-peroxidase protein (KatG) by blast search was separated. Immunoreactivity studies of purified KatG proteins with mice sera confirmed it to be specific for M. avium infection. Indirect ELISA with patient samples further confirmed it to be M. avium infection specific. CONCLUSION:KatG protein is specifically recognised by MAC patients and can be used as a marker for simple and rapid ELISA based tests for differential diagnosis of M. avium infection.
journal_name
Indian J Med Microbioljournal_title
Indian journal of medical microbiologyauthors
Gupta K,Verma I,Khuller G,Mahajan Rdoi
10.4103/0255-0857.66478subject
Has Abstractpub_date
2010-07-01 00:00:00pages
221-6issue
3eissn
0255-0857issn
1998-3646pii
IndianJMedMicrobiol_2010_28_3_221_66478journal_volume
28pub_type
杂志文章abstract:PURPOSE:To describe the changes in antibiotic susceptibility patterns of common intensive care unit pathogens with time from the medical intensive care unit of a tertiary care hospital. METHODS:A prospective observational study was conducted in the medical intensive care unit (MICU) of a 2100 bed tertiary care hospita...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.34764
更新日期:2007-07-01 00:00:00
abstract:PURPOSE:The intestinal flagellate Giardia lamblia includes many genetically distinct assemblages, of which assemblage A and B, predominantly infect humans. Nitroimidazoles derivatives (metronidazole and tinidazole) and nitazoxanide are some of the therapeutic agents for treatment of giardiasis. Nevertheless, some indiv...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.142236
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:Pneumonia is a preventable cause of mortality in children. Streptococcus pneumoniae colonising the nasopharynx of healthy children can cause invasive diseases and the serotype distribution of colonisation isolates should be an indicator of invasive disease, antibiotic resistance profiles, and potential vacci...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_15_347
更新日期:2017-10-01 00:00:00
abstract::Humanity has been at the receiving end of many viral diseases since ages. Sudden emergence and re-emergence of new viral diseases in human beings has surprised the medical scientists from time to time. "Avian influenza" or "Bird flu" by H5N1 epidemics is one such surprise. Although many aspects about this disease are ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2004-07-01 00:00:00
abstract::Three cases of external ophthalmomyiasis are reported here. The larvae were identified to be Oestrus ovis in two cases and Cochliomyia hominivorax in one. Two of the patients were immunocompetent while one was undergoing treatment for squamous cell carcinoma of eyelid. In the latter myiasis led to complete destruction...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.66490
更新日期:2010-07-01 00:00:00
abstract:Background:The WHO Multimodal Hand Hygiene Improvement Strategy (MHHIS) has been proposed to improve the Hand Hygiene (HH) compliance of the WHO recommendations on HH.Therefore, the current study was planned in our neonatal unit with the objective of evaluating the effectiveness of a Hand Hygiene Promotional Program (H...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_20_47
更新日期:2019-10-01 00:00:00
abstract::An attempt was made to speciate 102 clinically significant isolates of coagulase negative staphylococci (CoNS) by a practical scheme adapted from various references. This scheme utilizes slide and tube coagulase test, urease test ornithine decarboxylase, novobiocin susceptibility and aerobic acid from mannose for assi...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::Chikungunya (CHIK) fever is a re-emerging viral disease characterized by abrupt onset of fever with severe arthralgia followed by constitutional symptoms and rash lasting for 1-7 days. The disease is almost self-limiting and rarely fatal. Chikungunya virus (CHIKV) is a RNA virus belonging to family Togaviridae, genus ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.4103/0255-0857.38850
更新日期:2008-01-01 00:00:00
abstract:PURPOSE:Humans are the only known natural hosts of hepatitis C virus (HCV). This study was undertaken to examine the frequencies of human leucocyte antigens (HLAs) Class I and Class II genotype profiles in anti-HCV-infected patients of Northern India. MATERIALS AND METHODS:From a period of January 2013 to August 2014,...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.188317
更新日期:2016-07-01 00:00:00
abstract:PURPOSE:To find out the most suitable serological investigative procedures to diagnose dengue cases effectively in the laboratory practice identifying primary and secondary cases as well as period of suffering. MATERIALS AND METHODS:Dengue suspected cases sent to the laboratory in 2012 in central Kolkata by the local ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.136563
更新日期:2014-07-01 00:00:00
abstract::Bacterial infections of the eye are common and ophthalmologists are spoilt for choice with a variety of antibiotics available in the market. Antibiotics can be administered in the eye by a number of routes; topical, subconjunctival, subtenon and intraocular. Apart from a gamut of eye drops available, ophthalmologists ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.4103/0255-0857.83903
更新日期:2011-07-01 00:00:00
abstract:Objectives:Levonadifloxacin is a novel benzoquinolizine subclass of quinolone with broad-spectrum activities against problematic pathogens such as methicillin-resistant Staphylococcus aureus, quinolone-resistant S. aureus, vancomycin intermediate S. aureus, and vancomycin-resistant S. aureus. Levonadifloxacin and its o...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_20_307
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Hepatitis C is global health problem affecting a significant portion of the world's population. Available data in Western Maharashtra on seroprevalence, risk factors and genotype distribution are very limited. OBJECTIVES:The present study was carried out to estimate the seroprevalence, factor...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_16_96
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Surveillance of antimicrobial resistance (AMR) is of great importance. Pseudomonas aeruginosa and Acinetobacter baumannii are important pathogens and emergence of resistance in these have increased the morbidity and mortality rates. This surveillance study was initiated by the Government of India - Indian Co...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,多中心研究
doi:10.4103/0255-0857.195376
更新日期:2016-10-01 00:00:00
abstract::Invasive candidiasis is a life-threatening complication of critically ill immunocompromised patients with high attributable mortality. Due to non-specific clinical presentation, early detection of candidemia and accurate identification of Candida species are essential pre-requisites for improved therapeutic outcome. S...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.4103/0255-0857.99482
更新日期:2012-07-01 00:00:00
abstract::Rhinosporidiosis and its causative pathogen Rhinosporidium seeberi have been known for over a hundred years. Yet unresolved enigmas in rhinosporidiosis include the mode of infection, mechanisms of spread, mechanisms of immunity, some aspects of histopathology e.g. the significance of transepidermal elimination of spor...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract::Varied clinical presentations of Penicillium marneffei, an opportunistic pathogen in HIV disease has been rarely described in literature. We report a patient with advanced AIDS who presented to us with prolonged fever and had features of an acute abdomen. On radiologic imaging he had features of intestinal obstruction...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.40538
更新日期:2008-04-01 00:00:00
abstract::Salmonella Typhi has rarely been associated with focal abscesses; and in literature, there is no evidence of its association with abscesses in the neck spaces. Ability of Salmonella Typhi to invade and localise in the neck spaces not only poses a diagnostic challenge but also underscores the necessity to understand th...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.154889
更新日期:2015-04-01 00:00:00
abstract::Ocular nocardiosis is an opportunistic infection and is believed to be a rare entity. We describe a rare case report of a patient with culture-positive Nocardia asteroides canaliculitis who presented with complaints of watering, purulent discharge and painful swelling of left lower eye lid. A purulent tenacious materi...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2004-04-01 00:00:00
abstract:PURPOSE:Enteric fever is endemic in India with Salmonella Typhi being the major causative agent. Antibiotic therapy constitutes the mainstay of management. The present study was undertaken to find the susceptibility profile of Salmonella enterica var Typhi (S. Typhi) blood isolates in a tertiary care hospital between J...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.129799
更新日期:2014-04-01 00:00:00
abstract:PURPOSE:To compare BACTEC MGIT 960 (M960) with conventional culture on Lowenstein Jensen (LJ) media and direct acid fast bacilli (AFB) smear examination for the detection of Mycobacteria in clinical samples obtained from suspected cases of pulmonary and extra pulmonary tuberculosis (TB). METHODS:A total of 500 samples...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.37344
更新日期:2007-10-01 00:00:00
abstract:PURPOSE:To determine the risk factors, microbiological features, clinical features and other epidemiological characteristics of Nocardia keratitis seen at a tertiary eye care centre in south India. METHODS:We evaluated 31 patients with Nocardia keratitis seen over two years, from September 1999 to September 2001. Corn...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:PURPOSE:Virulence genes play important roles in pathogenesis of infections caused by S. aureus. The aim of this study was to determine the prevalence of PVL, eta and mecA genes in S. aureus isolated from patients in South-Western Nigeria. MATERIALS AND METHODS:In this study, a total of 116 S. aureus isolates from the ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.96675
更新日期:2012-04-01 00:00:00
abstract:PURPOSE:To evaluate the utility of the polymerase chain reaction (PCR) test for diagnosing osteoarticular tuberculosis (TB). METHODS:Clinical samples (synovial tissue and synovial fluid) obtained from 23 cases of suspected osteoarticular tuberculosis were subjected to Ziehl Neelsen (ZN) smear examination, radiometric ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2005-10-01 00:00:00
abstract::Extended spectrum -lactamases (ESBLs) are plasmid mediated, TEM and SHV derived enzymes, first isolated in Western Europe in mid 1980s, most commonly in Klebsiella spp., followed by Escherichia coli. These enzymes are capable of hydrolyzing broad spectrum cephalosporins and monobactams but inactive against cephamycins...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2004-04-01 00:00:00
abstract:PURPOSE:To determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) for the extracts of the leaves and seeds of the plant Azadirachta indica against various dermatophytes. METHODS:Clinical isolates of dermatophytes(Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2003-04-01 00:00:00
abstract:PURPOSE:A statistical assessment of a disease is often necessary before resources can be allocated to any control programme. No literature on seasonal trends of gonorrhoea is available from India. OBJECTIVES:The objectives were (1) to determine, if any, seasonal trends were present in India (2) to describe factors con...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.154853
更新日期:2015-04-01 00:00:00
abstract::Out of 210 faecal samples collected from children below 5 years attending different hospitals in Jammu and exhibiting clinical signs of diarrhoea, 41.9% samples were found positive for group A rotavirus by RNA-PAGE. Escherichia coli isolated in the study belonged to nine serogroups, out of which O69 was most frequent,...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.124308
更新日期:2014-01-01 00:00:00
abstract::The detection of extended-spectrum beta-lactamases (ESBLs) in gram-negative bacteria that produce AmpC beta-lactamases is problematic. In the present study, the performance of modified double-disc synergy test (MDDST) that employs a combination of cefepime and piperacillin-tazobactam for the detection of Proteus mirab...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.38860
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Mycobacterial antigen MPB64 is a secretory protein specific for Mycobacterium tuberculosis complex. A lateral flow immunochromatographic assay (ICA) is a method used for the rapid differentiation of M. tuberculosis complex. AIM:We aimed to evaluate the performance of ICA in rapid differentiation of M. tuber...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.71817
更新日期:2010-10-01 00:00:00